Standard name
Human Ortholog
Description Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within N-terminus is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress; human homolog CCS can complement yeast ccs1 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.98 0.92 0.91 0.82 0.76 0.83 0.81 0.79 0.82 1.0 0.98 0.98 0.97 0.97 1.0 0.97 0.94 0.95 0.93 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.14 0.19 0.08 0.11 0.08 0.1 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 4 2 1 7 1 7 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 0 2 1 2 14 3 7 11 10 15 12 2 1 1 0 1 3 0 0 0 0 0 0
Cytoplasm 363 191 209 209 241 449 244 389 305 272 314 331 158 275 286 109 100 94 355 176 205 132 260 235
Endoplasmic Reticulum 4 1 1 1 6 17 3 19 15 18 18 9 0 2 2 2 4 3 0 0 2 1 1 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1 0 0
Golgi 1 0 0 0 0 0 0 8 1 1 2 1 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2 0 7 0 10 4 11 8 0 2 1 0 1 0 0 0 1 0 0 1
Nucleus 2 0 2 1 0 17 9 5 5 10 18 12 0 0 1 7 0 2 4 4 1 2 1 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 2 3 1 19 11 42 95 31 38 31 39 0 5 2 0 0 2 1 5 0 1 3 2
Unique Cell Count 366 192 211 213 262 496 297 512 368 336 397 403 158 281 292 112 103 94 365 188 217 143 272 252
Labelled Cell Count 374 194 217 213 272 512 311 524 385 354 416 418 160 286 294 118 109 104 365 188 217 143 272 252


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.0 21.2 22.1 22.0 16.6 18.9 11.6 17.5 15.1 15.5 15.1 14.8 25.8 22.8 23.1 24.5 28.2 30.7 21.7 22.4 23.7
Std Deviation (1e-4) 4.3 4.3 4.5 4.6 3.7 3.6 3.0 3.2 2.8 2.7 2.7 2.8 5.4 4.6 4.8 6.0 9.3 9.4 5.4 4.8 5.4
Intensity Change (Log2) – – – -0.01 -0.41 -0.23 -0.93 -0.34 -0.55 -0.51 -0.55 -0.58 0.22 0.04 0.06 0.15 0.35 0.47 -0.03 0.02 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.0 0 0 0 0 0 0 0
Cytoplasm -0.8 -3.6 -4.1 -6.0 -7.5 -5.9 -6.3 -6.8 -6.1 1.2 -1.0 -1.0 -1.2 -1.3 0.9
Endoplasmic Reticulum 0 0 2.3 0 2.4 2.5 3.0 2.7 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 1.9 0 0 0 0 2.4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.0 0 5.0 6.1 3.4 4.3 3.3 3.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.3669 26.5495 23.3326 21.2142 19.0515 22.6407 22.6064 26.7667 24.0758 26.8878 23.8241 25.6976 22.0228 26.9678 23.9973 21.5342 22.48 24.5766
Actin 0.0094 0.0001 0.0001 0.0001 0.0363 0.0004 0.013 0.0006 0.0011 0 0.0034 0.0001 0.0183 0.0001 0.0024 0.0013 0.0016 0.0001
Bud 0.0004 0 0.0001 0 0.0005 0.0002 0.0003 0.0004 0.0009 0 0.0001 0 0.0002 0 0.0002 0.0002 0.0003 0.0003
Bud Neck 0.0012 0.0001 0.0002 0.0003 0.001 0.0006 0.0005 0.0007 0.0019 0.0001 0.0003 0.0005 0.0006 0.0001 0.0003 0.0003 0.0007 0.0005
Bud Periphery 0.0003 0 0.0001 0 0.0011 0.0001 0.0002 0.0002 0.0005 0 0.0001 0 0.0001 0 0.0002 0.0003 0.0004 0.0003
Bud Site 0.0038 0 0 0 0.0009 0.0001 0.0029 0.0045 0.0001 0 0.0002 0 0.0005 0 0.0005 0.0007 0.0004 0
Cell Periphery 0.0001 0 0 0 0.0002 0 0.0001 0.0001 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.8612 0.9801 0.9719 0.9716 0.7822 0.9306 0.8645 0.9799 0.9598 0.992 0.9435 0.9692 0.9088 0.9918 0.9461 0.9009 0.8753 0.9671
Cytoplasmic Foci 0.0182 0.0001 0.0002 0.0003 0.0063 0.0007 0.0206 0.0028 0.0076 0.0002 0.0052 0.0018 0.0052 0.0002 0.0062 0.0025 0.0153 0.0004
Eisosomes 0.0001 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0025 0.0002 0.0004 0.0009 0.0036 0.0015 0.0038 0.0006 0.0009 0.0001 0.0026 0.0009 0.0025 0.0002 0.0014 0.0009 0.0037 0.0008
Endosome 0.0061 0 0.0006 0.0003 0.0161 0.0008 0.0199 0.0003 0.0009 0 0.0095 0.0024 0.0038 0 0.0039 0.0086 0.02 0.0004
Golgi 0.0022 0 0 0 0.008 0 0.0051 0.0001 0.0002 0 0.0017 0.0001 0.0018 0 0.001 0.0022 0.005 0
Lipid Particles 0.006 0 0 0 0.0344 0 0.0063 0.0002 0.0023 0 0.0036 0.0007 0.0017 0 0.0027 0.004 0.0381 0
Mitochondria 0.0102 0 0.0009 0.0001 0.0125 0.0005 0.0021 0.0002 0.0011 0 0.0006 0.0001 0.0006 0 0.0045 0.0316 0.0115 0.0044
None 0.0037 0.0001 0.0002 0.0003 0.0042 0.0005 0.0054 0.001 0.0018 0.0002 0.0036 0.0007 0.0025 0.0003 0.0018 0.0005 0.0028 0.0003
Nuclear Periphery 0.0115 0.0024 0.004 0.0033 0.0278 0.0085 0.0086 0.0006 0.0014 0.0005 0.0104 0.0029 0.0055 0.0007 0.0026 0.0023 0.0022 0.0019
Nucleolus 0.0006 0 0.0001 0 0.0047 0 0.001 0.0002 0.0003 0 0.0001 0.0001 0.0006 0 0.0008 0.0002 0.0008 0
Nucleus 0.049 0.0167 0.0183 0.0219 0.0244 0.0537 0.0305 0.0061 0.0159 0.0066 0.0106 0.0186 0.0376 0.0065 0.0182 0.0381 0.0144 0.0224
Peroxisomes 0.0067 0 0 0 0.0108 0 0.0049 0.0001 0.001 0 0.0007 0 0.0012 0 0.0008 0.001 0.0028 0
Punctate Nuclear 0.0032 0.0001 0.0001 0.0002 0.0205 0.0004 0.008 0.0012 0.0006 0.0001 0.0025 0.0011 0.0073 0 0.0054 0.0002 0.0011 0.0002
Vacuole 0.002 0.0001 0.0016 0.0006 0.0016 0.0011 0.0016 0.0004 0.0013 0 0.0005 0.0004 0.0006 0.0001 0.0005 0.0026 0.0024 0.0005
Vacuole Periphery 0.0017 0 0.001 0.0001 0.0024 0.0004 0.0006 0 0.0004 0 0.0007 0.0002 0.0002 0 0.0003 0.0013 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 90.4792 144.6685 139.5554 133.5627 117.3102 114.6905 139.9578 134.6253 152.4931 112.9448
Translational Efficiency 2.366 1.9417 2.2063 2.807 2.6265 1.9021 1.8224 2.6972 1.8964 2.1265

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1396 1014 1876 1771 1389 2348 136 1368 2785 3362 2012 3139

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1624.79 2099.00 2197.41 1998.67 1837.83 2158.09 2444.07 2117.00 1731.04 2140.27 2214.08 2050.24
Standard Deviation 248.33 378.84 354.76 418.59 338.57 343.44 374.44 384.65 315.32 355.52 361.47 408.39
Intensity Change Log 2 — 0.369449 0.435551 0.298787 — 0.231752 0.411282 0.204018 — 0.298004 0.422721 0.249263

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000234 0.000336 0.000112 0.002204 0.000246 0.000117 0.000866 0.000165 0.000240 0.000183 0.000163 0.001316
Bud Neck 0.000947 0.000988 0.000879 0.007852 0.000741 0.000451 0.004199 0.000720 0.000844 0.000613 0.001104 0.004744
Bud Site 0.003138 0.003349 0.000736 0.022776 0.003829 0.001972 0.003509 0.001437 0.003483 0.002388 0.000923 0.013476
Cell Periphery 0.000120 0.000201 0.000112 0.000229 0.000280 0.000055 0.000940 0.000151 0.000200 0.000099 0.000168 0.000195
Cytoplasm 0.823724* 0.724877* 0.527161* 0.562233* 0.751253* 0.780467* 0.332201* 0.662616* 0.787579* 0.763701* 0.513983* 0.605981*
Cytoplasmic Foci 0.017690 0.010528 0.002182 0.013927 0.005239 0.004855 0.016055 0.002739 0.011480 0.006566 0.003119 0.009051
Eisosomes 0.000002 0.000002 0.000001 0.000013 0.000001 0.000001 0.000008 0.000002 0.000002 0.000001 0.000001 0.000008
Endoplasmic Reticulum 0.000500 0.000369 0.000075 0.000733 0.000252 0.000256 0.000402 0.000130 0.000376 0.000290 0.000097 0.000470
Endosome 0.000546 0.000639 0.000068 0.014265 0.000245 0.000380 0.000377 0.000194 0.000396 0.000458 0.000089 0.008133
Golgi 0.001059 0.001020 0.000124 0.008161 0.001622 0.000595 0.000620 0.001118 0.001340 0.000723 0.000158 0.005092
Lipid Particles 0.000835 0.000435 0.000222 0.002248 0.000158 0.000150 0.001960 0.000242 0.000497 0.000236 0.000339 0.001374
Mitochondria 0.000149 0.000358 0.000284 0.004901 0.000618 0.000131 0.000593 0.000137 0.000383 0.000199 0.000305 0.002824
Mitotic Spindle 0.004247 0.004765 0.004842 0.075963 0.004009 0.002895 0.003315 0.007548 0.004129 0.003459 0.004738 0.046147
None 0.005026 0.008000 0.012031 0.014176 0.007535 0.012670 0.006034 0.016032 0.006278 0.011261 0.011626 0.014985
Nuclear Periphery 0.000123 0.000156 0.000275 0.000591 0.000077 0.000075 0.000920 0.000141 0.000100 0.000099 0.000319 0.000395
Nuclear Periphery Foci 0.002931 0.001507 0.000450 0.002569 0.000536 0.000774 0.003767 0.001092 0.001736 0.000995 0.000675 0.001926
Nucleolus 0.000101 0.000142 0.000098 0.001464 0.000095 0.000088 0.000186 0.001146 0.000098 0.000104 0.000104 0.001325
Nucleus 0.130115* 0.236109* 0.445099* 0.206584* 0.215306* 0.188415* 0.593818* 0.294338* 0.172603* 0.202800* 0.455151* 0.244828*
Peroxisomes 0.000048 0.000049 0.000010 0.001903 0.000022 0.000050 0.000057 0.000072 0.000035 0.000050 0.000013 0.001105
Vacuole 0.008044 0.005612 0.005205 0.049722 0.002512 0.005191 0.030057 0.008386 0.005285 0.005318 0.006885 0.031707
Vacuole Periphery 0.000421 0.000559 0.000035 0.007486 0.005422 0.000413 0.000115 0.001593 0.002915 0.000457 0.000040 0.004918

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.99 0.89 -1.68 -0.95 -1.87 3.78 -8.43 -0.30 -1.77 7.01 1.55 -1.44 -1.67 -2.09 -1.32
Bud Neck -1.52 -3.10 -6.63 -5.68 -4.67 2.55 -8.35 -6.00 -8.21 7.44 1.41 -7.19 -8.28 -9.36 -1.50
Bud Site -0.15 5.79 -3.98 -2.78 -6.86 2.58 -3.45 1.52 -1.83 5.12 2.35 4.70 -2.38 -4.47 -6.79
Cell Periphery -5.30 -4.81 -2.11 3.65 2.84 3.92 -8.03 1.84 -6.15 9.40 2.94 -2.82 1.18 -4.07 6.51
Cytoplasm 13.97 49.77 36.16 17.15 -8.88 -2.77 26.77 16.33 22.57 -18.74 6.93 50.56 36.36 32.11 -16.68
Cytoplasmic Foci 3.61 11.38 8.81 5.21 -3.87 0.03 -7.33 -0.34 -0.44 7.31 5.61 7.81 7.56 2.48 -0.49
Eisosomes 0.52 -0.29 -8.65 -9.09 -9.19 -1.77 -8.82 -8.50 -7.05 7.53 0.41 -7.13 -11.41 -11.94 -5.77
Endoplasmic Reticulum 0.85 7.02 3.93 0.48 -5.45 0.13 -4.56 4.08 1.14 7.57 1.05 6.93 5.14 0.75 -2.05
Endosome -0.01 6.30 -3.29 -2.84 -4.25 -0.88 -3.88 1.22 1.87 5.25 0.03 4.97 -3.22 -3.05 -4.13
Golgi 0.20 5.86 -0.41 -0.48 -2.00 2.63 1.72 2.68 0.41 2.08 2.70 5.29 1.15 -0.67 -2.02
Lipid Particles 0.78 0.72 -1.68 -5.47 -5.95 0.02 -7.47 -8.22 -9.09 6.52 1.41 -2.40 -3.48 -10.41 -2.01
Mitochondria -1.33 -11.17 -2.69 -1.86 -1.87 7.49 0.38 7.11 -2.13 9.04 3.57 -0.60 -1.29 -2.42 -1.18
Mitotic Spindle 0.77 -0.69 -5.19 -5.48 -4.82 1.38 0.03 -0.86 -1.88 -0.64 1.87 -1.39 -5.21 -6.32 -3.81
None -5.18 -13.67 -5.28 -1.22 3.72 -6.14 4.50 -6.31 -0.53 -10.88 -9.11 -11.06 -7.65 0.71 1.01
Nuclear Periphery -1.42 -15.84 -17.09 -13.45 -3.99 -1.39 -11.38 -15.57 -16.51 9.00 -0.78 -21.15 -21.76 -21.48 2.34
Nuclear Periphery Foci 2.62 5.10 3.80 1.83 -3.77 -3.45 -5.72 -3.41 -1.98 4.68 2.71 2.42 1.62 -1.48 -1.07
Nucleolus 0.16 0.05 -3.45 -3.81 -3.84 -0.06 -10.80 -1.25 -1.25 -0.70 0.57 -1.41 -2.71 -2.86 -2.48
Nucleus -16.48 -53.72 -20.50 -0.13 31.81 3.18 -14.85 -11.06 -16.73 10.61 -8.61 -49.31 -20.19 -13.15 32.32
Peroxisomes 0.54 4.45 -2.58 -2.65 -2.95 -1.67 -6.76 -1.27 0.20 3.45 -0.63 3.12 -2.74 -2.60 -3.00
Vacuole 0.49 -6.07 -27.18 -26.89 -24.66 -7.21 -8.42 -16.85 -13.59 4.44 -3.43 -12.25 -30.85 -29.36 -21.89
Vacuole Periphery -0.33 2.80 -1.97 -1.59 -3.14 3.47 3.61 3.31 -0.23 -0.70 3.40 3.92 2.67 -1.55 -3.00
Standard name
Human Ortholog
Description Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within N-terminus is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress; human homolog CCS can complement yeast ccs1 null mutant
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ccs1

Ccs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available