Standard name
Human Ortholog
Description Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4)
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.05 0.06 0 0 0 0.06 0 0 0 0 0.1 0.16 0 0 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleus 0.94 0.9 0.94 0.93 0.91 0.91 0.88 0.89 0.83 0.87 0.83 0.84 0.94 0.86 0.74 0.95 0.95 – 0.93 0.91 0.9 0.92 0.77 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.11 0.06 0.12 0.16 0.18 0.17 0 0 0.07 0 0.05 – 0 0 0 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.14 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 1 1 0 0 0
Bud 0 1 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0 – 0 2 0 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 1 0 0 0 2 2 0 3 3 1 4 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 5 16 17 24 18 21 28 23 11 14 12 10 30 48 0 0 – 0 1 1 2 3 2
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0
Endosome 0 1 1 1 0 0 1 0 0 2 0 1 1 0 4 0 0 – 0 1 4 0 2 3
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 1 1 0 0 – 0 1 3 0 0 0
Mitochondria 1 5 0 2 0 7 4 4 29 11 24 7 0 2 0 0 0 – 1 3 2 0 2 5
Nucleus 67 183 298 299 364 534 679 578 327 390 352 350 213 256 220 20 37 – 82 189 296 139 213 220
Nuclear Periphery 2 1 0 0 0 4 9 2 2 8 4 9 1 3 4 0 0 – 0 0 1 0 0 5
Nucleolus 3 3 0 4 4 36 86 39 48 72 75 69 2 11 20 0 2 – 0 0 1 2 10 30
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 1 0 – 0 0 0 0 0 1
SpindlePole 0 2 0 0 0 0 1 3 0 1 0 1 1 2 8 0 0 – 0 0 0 0 0 2
Vac/Vac Membrane 0 7 3 4 10 9 8 14 5 2 7 7 1 2 4 0 0 – 1 6 10 3 38 70
Unique Cell Count 71 203 316 323 398 587 770 648 395 448 426 415 227 297 297 21 39 89 209 330 152 276 350
Labelled Cell Count 73 210 318 328 404 610 811 669 438 502 479 461 229 307 313 21 39 89 209 330 152 276 350


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 8.5 10.2 10.0 11.0 9.3 8.9 9.0 8.0 8.0 8.0 8.4 10.9 10.7 10.1 9.4 10.5 – 10.1 10.5 10.5
Std Deviation (1e-4) 1.8 1.9 1.9 2.3 2.5 2.0 2.2 3.8 2.1 1.8 2.0 2.1 2.0 3.2 2.9 2.1 2.2 – 2.0 1.9 2.0
Intensity Change (Log2) – – – -0.04 0.1 -0.14 -0.21 -0.18 -0.35 -0.35 -0.36 -0.29 0.09 0.06 -0.03 -0.12 0.03 – -0.02 0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Cytoplasm 0.1 0.6 -1.5 -1.9 -0.5 0.4 -1.9 -1.2 -1.5 -0.4 2.4 4.5 0 0 –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Mitochondria 0 0 0 0 0 4.9 0 4.3 0 0 0 0 0 0 –
Nucleus -0.9 -1.5 -1.8 -3.0 -2.6 -4.7 -3.3 -4.8 -4.2 -0.2 -3.4 -6.9 0.2 0.1 –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Nucleolus 0 0 4.5 6.2 4.5 6.4 7.5 7.9 7.6 0 3.5 4.7 0 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 –
Vacuole 0 1.6 0 0 1.3 0 0 0 0 0 0 0 0 0 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.756 5.4433 5.1263 4.8517 6.1353 5.7744 6.0494 8.2138 6.4352 6.152 5.2402 6.9504 5.3496 8.1774 6.8341 6.2645 6.1864 6.8419
Actin 0.0188 0 0.0089 0.0003 0.0001 0.0097 0.0636 0 0.0024 0 0.0055 0.0005 0.0641 0 0 0.0377 0 0
Bud 0.001 0 0.0007 0.0008 0.0001 0.0001 0.0004 0 0.0001 0 0.0003 0.0001 0.0006 0 0 0.0005 0 0.0001
Bud Neck 0.0028 0.0001 0.0012 0.0001 0.0006 0.0011 0.0006 0 0.0002 0 0.0005 0.0005 0.0017 0 0 0.0005 0 0.0005
Bud Periphery 0.0018 0 0.0013 0.0035 0.0004 0.0004 0.001 0 0.0001 0 0.0005 0.0002 0.001 0 0 0.0004 0 0.0001
Bud Site 0.0053 0.0001 0.0164 0.0002 0.0001 0.0002 0.0011 0 0.0005 0 0.0011 0.0001 0.0013 0 0 0.0046 0 0
Cell Periphery 0.0013 0 0.0006 0.0003 0.0002 0.0002 0.0004 0 0.0001 0 0.0002 0.0001 0.0003 0 0 0 0 0.0001
Cytoplasm 0.0289 0.0061 0.0014 0.0154 0.0001 0.0001 0.0045 0.0001 0.0029 0 0.0008 0.0018 0.0107 0 0 0.0005 0 0
Cytoplasmic Foci 0.0053 0.0001 0.0079 0.0033 0 0.0008 0.0082 0 0.0156 0 0.0171 0.013 0.027 0 0 0.0148 0 0
Eisosomes 0.0002 0 0.0001 0.0001 0 0.0001 0.0004 0 0 0 0.0002 0 0.0004 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0017 0.0002 0.0007 0.0123 0.0001 0.0003 0.0138 0 0.0004 0 0.0015 0.0006 0.0044 0 0 0.0002 0 0
Endosome 0.0105 0.0005 0.0205 0.0584 0.0001 0.0027 0.0108 0 0.0069 0 0.0278 0.007 0.0182 0 0 0.002 0 0
Golgi 0.0033 0 0.006 0.003 0 0.0018 0.0111 0 0.0042 0 0.0623 0.0002 0.0085 0 0 0.0036 0 0
Lipid Particles 0.012 0 0.0239 0.0009 0 0.0079 0.0015 0 0.009 0 0.0246 0.0009 0.0146 0 0 0.004 0 0
Mitochondria 0.0056 0.0003 0.0164 0.0288 0.0012 0.0081 0.0066 0.0004 0.0037 0.0003 0.027 0.0016 0.0204 0.0003 0.0003 0.0005 0.0004 0.0002
None 0.0081 0.0045 0.0005 0.0096 0 0.0002 0.0039 0 0.0013 0 0.0016 0.0004 0.0054 0 0 0.0005 0 0
Nuclear Periphery 0.0109 0.0184 0.0222 0.007 0.0082 0.0059 0.0369 0.0028 0.0029 0.0006 0.0085 0.0093 0.0088 0.0005 0.0011 0.0007 0.0006 0.0004
Nucleolus 0.0304 0.0126 0.0212 0.0193 0.1359 0.0949 0.0122 0.0123 0.0234 0.0085 0.1538 0.038 0.0186 0.008 0.0091 0.01 0.018 0.0394
Nucleus 0.8352 0.953 0.8227 0.8142 0.8495 0.8615 0.8102 0.9836 0.9126 0.9903 0.6508 0.9151 0.7725 0.9907 0.9889 0.8991 0.9808 0.9587
Peroxisomes 0.0036 0 0.0106 0.0001 0 0.0015 0.0013 0 0.0028 0 0.004 0.005 0.0078 0 0 0.0089 0 0
Punctate Nuclear 0.0056 0.0012 0.0082 0.0003 0.0002 0.0008 0.0004 0.0002 0.01 0.0001 0.0073 0.0012 0.0098 0 0 0.0111 0 0
Vacuole 0.0054 0.002 0.0056 0.0129 0.0013 0.0007 0.0053 0.0002 0.0004 0 0.0015 0.0024 0.0015 0.0002 0.0001 0.0001 0 0.0003
Vacuole Periphery 0.0024 0.0009 0.0028 0.009 0.0017 0.001 0.0057 0.0004 0.0005 0 0.003 0.0019 0.0024 0.0002 0.0003 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.553 19.0767 29.1441 32.9873 29.2958 31.365 24.3942 33.6946 36.176 27.229
Translational Efficiency 6.662 4.6323 2.5544 2.5735 2.7795 2.7409 3.0548 2.3975 2.2219 2.9763

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1531 451 2452 1320 257 1966 1844 1725 1788 2417 4296 3045

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 884.67 1080.04 1172.18 1460.75 917.37 1031.34 1028.76 1465.30 889.37 1040.43 1110.62 1463.33
Standard Deviation 142.09 167.09 180.83 288.62 121.23 154.34 219.67 317.40 139.75 157.94 210.75 305.27
Intensity Change Log 2 — 0.287873 0.405983 0.723498 — 0.168944 0.165331 0.675620 — 0.228555 0.288489 0.699323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000344 0.000533 0.000432 0.000504 0.000337 0.000388 0.000455 0.000449 0.000343 0.000415 0.000442 0.000473
Bud Neck 0.005153 0.009633 0.011765 0.008616 0.002130 0.008427 0.008331 0.008995 0.004718 0.008652 0.010291 0.008831
Bud Site 0.000565 0.001914 0.000655 0.000842 0.001388 0.001784 0.000988 0.000742 0.000683 0.001808 0.000798 0.000786
Cell Periphery 0.000160 0.000071 0.000085 0.000123 0.000021 0.000215 0.000348 0.000126 0.000140 0.000188 0.000198 0.000124
Cytoplasm 0.001055 0.000238 0.000106 0.000453 0.003073 0.002259 0.003027 0.000340 0.001345 0.001882 0.001360 0.000389
Cytoplasmic Foci 0.000079 0.000035 0.000035 0.000615 0.000548 0.000499 0.000306 0.000037 0.000146 0.000412 0.000151 0.000288
Eisosomes 0.000036 0.000043 0.000067 0.000041 0.000018 0.000042 0.000042 0.000051 0.000034 0.000042 0.000056 0.000047
Endoplasmic Reticulum 0.005536 0.004009 0.001778 0.000962 0.002804 0.006270 0.002163 0.000987 0.005144 0.005848 0.001943 0.000976
Endosome 0.000284 0.000340 0.000134 0.000600 0.000209 0.000804 0.000230 0.000071 0.000273 0.000718 0.000175 0.000300
Golgi 0.000039 0.000056 0.000170 0.001208 0.000037 0.000282 0.000057 0.000063 0.000039 0.000240 0.000121 0.000560
Lipid Particles 0.000041 0.000060 0.000024 0.000134 0.000076 0.000274 0.000075 0.000007 0.000046 0.000234 0.000046 0.000062
Mitochondria 0.000706 0.001506 0.001170 0.001627 0.000351 0.002416 0.001314 0.001238 0.000655 0.002246 0.001232 0.001407
Mitotic Spindle 0.000122 0.003609 0.000154 0.001039 0.002188 0.001788 0.001061 0.000655 0.000419 0.002128 0.000543 0.000821
None 0.002498 0.000869 0.000434 0.001153 0.000898 0.003984 0.011119 0.000725 0.002268 0.003403 0.005021 0.000911
Nuclear Periphery 0.000265 0.000243 0.000326 0.000336 0.000079 0.000754 0.000339 0.000211 0.000239 0.000659 0.000332 0.000265
Nuclear Periphery Foci 0.000618 0.000110 0.000061 0.000708 0.000091 0.000333 0.000218 0.000103 0.000542 0.000291 0.000128 0.000366
Nucleolus 0.017177 0.019381 0.006838 0.008820 0.018335 0.015405 0.006081 0.006029 0.017344 0.016147 0.006513 0.007239
Nucleus 0.964171* 0.955898* 0.974566* 0.967862* 0.965721* 0.949907* 0.963004* 0.977819* 0.964394* 0.951025* 0.969603* 0.973503*
Peroxisomes 0.000146 0.000465 0.000135 0.000283 0.000347 0.000271 0.000235 0.000167 0.000175 0.000307 0.000178 0.000217
Vacuole 0.000582 0.000656 0.000672 0.002384 0.001254 0.002507 0.000444 0.000756 0.000679 0.002161 0.000574 0.001462
Vacuole Periphery 0.000422 0.000330 0.000395 0.001688 0.000096 0.001390 0.000162 0.000429 0.000376 0.001192 0.000295 0.000975

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.25 -2.62 -4.59 0.37 -1.82 -0.36 -1.43 -1.41 -2.14 -0.04 -2.11 -3.46 -4.64 -2.38 -1.36
Bud Neck -2.46 -8.47 -3.32 0.30 2.62 -8.10 -8.23 -7.63 -1.05 -1.03 -4.89 -8.68 -5.51 -0.76 1.51
Bud Site -1.45 -0.71 -1.45 0.90 -1.12 -0.33 0.41 0.55 1.86 0.85 -2.33 -0.56 -0.87 1.94 -0.58
Cell Periphery 2.40 2.26 0.79 -1.41 -1.17 -5.48 -3.96 -4.09 1.99 2.50 -1.20 -1.18 0.41 1.73 1.63
Cytoplasm 2.20 2.68 -0.18 -1.40 -1.61 0.45 0.17 1.43 3.32 4.23 -0.84 0.16 1.27 2.13 1.47
Cytoplasmic Foci 0.97 0.98 -1.78 -1.99 -2.00 0.22 0.67 1.36 2.32 1.03 -1.48 0.14 -0.92 0.61 -0.94
Eisosomes -1.44 -4.36 -1.42 0.36 3.59 -8.58 -6.81 -8.12 -1.96 -1.94 -2.96 -4.95 -4.10 -1.37 1.97
Endoplasmic Reticulum 2.15 7.07 8.42 6.10 4.82 -6.24 2.40 6.72 10.90 11.40 -1.15 7.04 9.15 11.94 10.21
Endosome -0.29 1.04 -1.17 -0.97 -1.94 -2.71 -0.01 1.09 4.12 2.51 -2.42 0.86 -0.18 2.39 -1.20
Golgi -0.97 -1.10 -2.08 -2.05 -1.81 -1.46 -0.69 -0.64 1.28 -0.33 -1.52 -1.20 -2.14 -1.13 -1.74
Lipid Particles -0.58 0.81 -1.32 -1.01 -1.58 -1.37 0.35 1.86 2.10 2.21 -1.78 0.20 -0.43 1.60 -0.55
Mitochondria -1.48 -2.23 -4.73 -0.32 -1.52 -3.91 -2.92 -11.95 2.16 0.09 -3.50 -2.87 -5.52 1.81 -0.79
Mitotic Spindle -1.47 -0.76 -1.69 0.89 -1.59 0.32 0.65 0.77 1.75 0.59 -2.35 -0.09 -0.79 1.71 -0.93
None 4.26 5.80 2.59 -0.82 -2.03 -3.95 -6.79 0.87 4.39 7.00 -1.67 -3.79 3.90 3.97 6.16
Nuclear Periphery 0.22 -0.40 -0.77 -0.86 -0.07 -4.62 -3.15 -4.11 3.62 1.43 -3.25 -0.91 -0.56 3.08 0.60
Nuclear Periphery Foci 1.42 1.56 -0.13 -1.18 -1.28 -1.56 -0.77 0.07 1.59 0.83 0.79 1.38 0.46 -0.33 -1.13
Nucleolus -0.87 8.12 5.27 4.03 -1.55 1.16 4.07 4.10 7.05 0.12 0.82 9.60 8.48 7.39 -0.84
Nucleus 1.48 -3.77 -0.30 -1.52 2.18 1.95 0.06 -1.96 -7.78 -4.51 4.20 -1.97 -2.88 -6.69 -1.34
Peroxisomes -5.26 0.37 -3.77 2.67 -4.32 1.19 1.54 3.51 1.73 0.81 -2.54 0.03 -1.70 1.73 -1.20
Vacuole -0.37 -0.57 -2.71 -2.55 -2.46 -1.22 0.88 0.48 3.47 -1.41 -3.84 0.43 -2.26 1.48 -2.67
Vacuole Periphery 0.49 0.15 -1.81 -1.95 -1.84 -3.72 -1.17 -1.67 2.34 -1.37 -2.68 0.55 -1.79 0.53 -2.08
Standard name
Human Ortholog
Description Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4)
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sub1

Sub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available