Standard name
Human Ortholog
Description Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.1 0.17 0.15 – 0.11 0.07 0.1 0.09 0.08 0.11 0.16 0.06 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.07 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.1 0 0 0 0.53 0.26 – 0.58 0.5 0.56 0.56 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nucleus 0.91 0.92 0.95 0.9 0.78 0.5 0.55 – 0.54 0.55 0.57 0.59 0.91 0.87 0.78 0.86 0.89 0.85 0.86 0.91 0.85 0.81 0.72 0.71
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 0.06 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.05 – 0 0 0 0 0 0 0 0.08 0.05 0.07 0 0 0.07 0.1 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 5 1 – 11 9 14 8 0 0 0 0 0 0 2 0 0 0 4 4
Bud Neck 0 0 0 0 1 2 1 – 5 8 6 0 0 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 0 7 3 – 10 6 22 28 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 10 19 35 69 72 – 61 42 50 50 23 51 76 7 3 3 0 1 1 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 1 0 0 0 1 2 1 1 0 3 0 0 0 0 0 1
Endosome 0 0 3 2 12 1 32 – 7 15 4 8 1 0 9 0 1 0 0 0 1 2 6 5
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 1 0 0 0 0 0 4 2
Mitochondria 5 15 2 13 13 219 120 – 310 280 285 325 2 16 1 6 5 1 2 0 3 1 3 11
Nucleus 63 131 224 260 287 209 254 – 290 311 288 346 275 402 365 102 98 39 76 134 208 194 320 313
Nuclear Periphery 1 1 0 0 0 1 0 – 2 0 0 3 2 0 0 1 1 1 0 0 1 0 3 2
Nucleolus 2 3 0 0 0 1 1 – 4 4 2 8 0 3 2 0 1 0 2 2 4 10 32 26
Peroxisomes 0 0 0 0 1 0 2 – 0 0 0 0 0 1 3 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 5 20 17 26 – 9 29 6 13 1 1 1 0 1 1 0 1 2 2 9 6
Vac/Vac Membrane 0 6 2 2 23 9 23 – 18 16 13 17 3 3 20 9 5 3 2 5 16 23 54 61
Unique Cell Count 69 143 237 289 368 415 466 534 561 507 582 303 463 468 118 110 46 89 148 246 239 448 442
Labelled Cell Count 73 158 245 301 392 540 535 728 720 690 806 309 480 478 127 116 51 89 148 246 239 448 442


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.2 6.0 5.6 5.8 3.9 4.7 – 3.8 4.1 4.0 4.2 6.2 5.5 6.1 7.8 7.7 7.8 5.6 5.7 5.8
Std Deviation (1e-4) 0.8 0.8 1.2 1.8 1.6 1.5 1.7 – 0.9 1.0 0.9 0.9 1.2 1.2 1.2 1.2 1.3 1.4 1.2 1.4 1.2
Intensity Change (Log2) – – – -0.1 -0.06 -0.62 -0.36 – -0.65 -0.57 -0.6 -0.53 0.04 -0.14 0.03 0.37 0.36 0.38 -0.11 -0.07 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 2.4 4.7 4.4 – 3.2 1.7 2.6 2.2 1.6 3.0 4.6 0.7 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.5 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 2.1 0 0 – 0 0 0 0 0 2.1 0 0 0 0
Nucleus -1.9 -5.5 -11.5 -10.7 – -10.9 -10.7 -10.3 -9.9 -1.6 -3.1 -5.6 -2.6 -1.8 -2.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.3 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.3 0 2.8 – 2.0 1.7 0 1.8 0 0 2.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6876 0.7937 0.4827 0.3746 0.1682 0.5923 1.2279 1.4979 1.2234 1.0282 1.3252 1.4138 4.1156 3.5081 3.6137 4.182 3.2159 3.8985
Actin 0.0238 0 0.0015 0 0 0.0003 0.0102 0.0003 0.007 0.0082 0.0008 0.004 0.0096 0 0.0335 0.0009 0 0.0038
Bud 0.0003 0 0 0 0 0.0001 0.0004 0 0 0.0001 0.0001 0 0.0008 0.0046 0.0002 0.0001 0 0.0002
Bud Neck 0.003 0.0001 0.0001 0 0.0003 0.0008 0.0012 0.0001 0.0006 0.0001 0.0004 0.0008 0.0131 0.0003 0.0004 0.0005 0.0003 0.0011
Bud Periphery 0.0006 0 0 0 0 0.0001 0.0016 0 0.0001 0.0001 0.0002 0.0001 0.0019 0.0042 0.0002 0.0002 0 0.0008
Bud Site 0.0013 0 0 0 0 0.0002 0.0011 0.0001 0.0002 0.0001 0.0011 0.0001 0.0052 0.0009 0.0005 0.0002 0 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0 0.0001 0 0.0001 0 0.0009 0.0002 0.0001 0 0 0.0001
Cytoplasm 0.0058 0.0013 0.0007 0.0001 0.0002 0.0004 0.0027 0.0008 0.0009 0.0007 0.0026 0.0002 0.0082 0.0092 0.0015 0.0006 0.0005 0.0003
Cytoplasmic Foci 0.0033 0 0.0002 0 0 0.0002 0.0037 0.0001 0.0017 0.0003 0.0034 0 0.0185 0.0007 0.0041 0 0 0.0015
Eisosomes 0.0003 0 0 0 0 0 0.0002 0 0.0006 0.0001 0.0001 0.0001 0.0002 0 0.0006 0 0 0
Endoplasmic Reticulum 0.0024 0 0.0002 0 0 0.0001 0.0019 0.0002 0.0008 0.001 0.0009 0.0003 0.0029 0.0002 0.0007 0.0002 0 0.0002
Endosome 0.0138 0 0.0008 0 0 0.0011 0.0076 0.0004 0.0011 0.0043 0.0301 0.0007 0.0245 0.0001 0.0047 0.0002 0 0.0034
Golgi 0.0041 0 0.0002 0 0 0.0001 0.0017 0.0001 0.0012 0.0011 0.0057 0.0007 0.0051 0 0.01 0 0 0.0016
Lipid Particles 0.006 0 0.0002 0 0 0.0002 0.0037 0.0005 0.0044 0.0005 0.0116 0.0002 0.0198 0 0.0039 0 0 0.0062
Mitochondria 0.0021 0.0001 0.0003 0 0.0001 0.0005 0.0066 0.0002 0.001 0.0031 0.0058 0.002 0.0043 0.0003 0.0072 0.0004 0.0001 0.0049
None 0.0032 0.0078 0.0049 0.0001 0.0003 0.0002 0.0045 0.0002 0.0031 0.0007 0.0079 0.0001 0.0108 0.0005 0.0086 0.0002 0.0001 0.0003
Nuclear Periphery 0.0128 0.0136 0.0054 0.0005 0.0018 0.004 0.0113 0.0083 0.0039 0.0127 0.0071 0.0037 0.0117 0.004 0.0022 0.0126 0.0032 0.0035
Nucleolus 0.0085 0.0107 0.0082 0.0046 0.0258 0.0188 0.0076 0.0064 0.01 0.0032 0.0288 0.0137 0.0177 0.006 0.0087 0.0098 0.0075 0.0169
Nucleus 0.8901 0.9643 0.9611 0.9944 0.966 0.9687 0.9135 0.9683 0.9495 0.9608 0.8739 0.963 0.8111 0.9576 0.8943 0.9661 0.9833 0.9424
Peroxisomes 0.0021 0 0.0001 0 0 0.0006 0.0015 0.0002 0.0092 0.0002 0.0011 0 0.0078 0 0.0101 0 0 0.0068
Punctate Nuclear 0.0141 0.002 0.0158 0.0003 0.0054 0.0035 0.0098 0.0133 0.0042 0.0021 0.0138 0.0096 0.0212 0.0092 0.0079 0.0076 0.0048 0.004
Vacuole 0.0015 0.0001 0.0001 0 0 0.0002 0.0065 0.0002 0.0003 0.0002 0.0023 0.0002 0.0037 0.0017 0.0002 0.0001 0 0.001
Vacuole Periphery 0.0008 0.0001 0.0001 0 0 0.0001 0.0022 0.0002 0.0002 0.0006 0.0024 0.0004 0.001 0.0003 0.0002 0.0002 0 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3315 12.93 20.5599 14.387 8.0371 26.847 23.8125 23.3199 16.1207 15.5994
Translational Efficiency 1.1435 0.9781 0.711 0.8791 1.3756 0.6882 0.4633 0.7803 0.9793 0.8488

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
938 320 2820 100 1607 2373 188 1831 2545 2693 3008 1931

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 705.59 898.11 901.21 1009.44 706.30 850.20 1011.86 942.19 706.04 855.89 908.13 945.67
Standard Deviation 86.76 126.81 113.14 117.65 90.86 111.22 109.52 126.32 89.37 114.24 116.05 126.77
Intensity Change Log 2 — 0.348062 0.353033 0.516653 — 0.267521 0.518657 0.415737 — 0.308333 0.438262 0.467052

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000364 0.000883 0.000272 0.000815 0.000150 0.000143 0.000775 0.000610 0.000229 0.000231 0.000303 0.000621
Bud Neck 0.001920 0.010160 0.014111 0.005430 0.002082 0.007113 0.003463 0.016216 0.002022 0.007475 0.013445 0.015658
Bud Site 0.000501 0.002216 0.001665 0.012710 0.000394 0.001555 0.001597 0.005629 0.000433 0.001634 0.001661 0.005995
Cell Periphery 0.000140 0.000121 0.000083 0.000116 0.000105 0.000107 0.000072 0.000125 0.000118 0.000108 0.000082 0.000125
Cytoplasm 0.021311 0.010426 0.004868 0.027751 0.022090 0.006907 0.006663 0.023440 0.021803 0.007325 0.004980 0.023663
Cytoplasmic Foci 0.007555 0.001238 0.000028 0.000097 0.002352 0.001467 0.000451 0.000993 0.004270 0.001440 0.000055 0.000947
Eisosomes 0.000034 0.000029 0.000015 0.000029 0.000016 0.000013 0.000050 0.000022 0.000023 0.000015 0.000017 0.000022
Endoplasmic Reticulum 0.002489 0.009560 0.003407 0.006894 0.001682 0.003142 0.007905 0.006675 0.001979 0.003905 0.003688 0.006686
Endosome 0.000434 0.000937 0.000211 0.000303 0.000352 0.000987 0.000627 0.001896 0.000383 0.000981 0.000237 0.001814
Golgi 0.000785 0.000265 0.000035 0.000057 0.000142 0.000524 0.000068 0.000403 0.000379 0.000493 0.000037 0.000385
Lipid Particles 0.002358 0.000694 0.000073 0.000266 0.001276 0.000335 0.000731 0.000294 0.001675 0.000377 0.000114 0.000293
Mitochondria 0.002861 0.002225 0.000999 0.001021 0.001644 0.003703 0.000648 0.003680 0.002093 0.003527 0.000977 0.003543
Mitotic Spindle 0.001056 0.006095 0.000747 0.023759 0.001987 0.003496 0.000530 0.012387 0.001644 0.003805 0.000734 0.012976
None 0.012428 0.006311 0.002699 0.004227 0.009994 0.005162 0.002697 0.006056 0.010891 0.005298 0.002699 0.005962
Nuclear Periphery 0.000930 0.001009 0.000805 0.000773 0.000801 0.001430 0.000607 0.002454 0.000849 0.001380 0.000792 0.002367
Nuclear Periphery Foci 0.000884 0.000555 0.000058 0.000144 0.000315 0.000307 0.000582 0.001318 0.000525 0.000337 0.000091 0.001257
Nucleolus 0.015996 0.009324 0.001852 0.001123 0.013038 0.007050 0.006372 0.002992 0.014128 0.007320 0.002135 0.002895
Nucleus 0.924933* 0.932507* 0.965798* 0.908780* 0.939964* 0.953708* 0.960061* 0.903863* 0.934424* 0.951189* 0.965439* 0.904118*
Peroxisomes 0.001955 0.000587 0.000047 0.000118 0.000605 0.000684 0.000343 0.000244 0.001102 0.000673 0.000065 0.000237
Vacuole 0.000848 0.003379 0.001903 0.005430 0.000857 0.001687 0.005624 0.009081 0.000854 0.001888 0.002136 0.008892
Vacuole Periphery 0.000220 0.001480 0.000326 0.000155 0.000153 0.000480 0.000136 0.001623 0.000178 0.000598 0.000314 0.001547

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.45 0.37 -5.25 -2.21 -6.10 0.16 -7.82 -11.63 -15.17 3.50 -0.13 -3.62 -10.10 -13.02 -9.40
Bud Neck -4.68 -13.83 -5.04 0.21 3.48 -7.87 -3.89 -17.28 -10.99 -10.53 -8.84 -15.64 -17.51 -10.76 -3.61
Bud Site -3.09 -3.49 -1.80 -1.35 -1.45 -3.77 -1.80 -6.97 -4.89 -2.02 -4.35 -3.99 -7.15 -5.04 -4.33
Cell Periphery 0.76 3.42 1.41 0.61 -1.21 0.01 0.36 0.01 -0.01 -0.40 0.51 2.99 1.15 0.09 -2.42
Cytoplasm 3.07 6.62 0.80 -1.00 -2.08 7.04 4.67 0.29 -7.95 -4.73 8.45 10.41 0.46 -7.82 -9.73
Cytoplasmic Foci 4.77 5.69 5.60 2.53 -3.30 1.44 0.44 3.94 2.01 1.06 4.43 7.30 6.39 2.20 -2.67
Eisosomes 1.41 7.71 -2.55 -3.24 -5.79 2.98 -5.85 -8.49 -12.21 4.68 6.37 2.87 -3.67 -11.39 -7.37
Endoplasmic Reticulum -6.51 -4.80 -6.34 -0.68 -5.36 -6.02 -8.91 -19.58 -12.95 2.51 -7.51 -11.49 -19.50 -11.04 -11.24
Endosome -1.41 4.13 -2.02 0.16 -3.50 -2.78 -2.12 -3.70 -1.05 0.12 -2.89 1.89 -3.68 -1.06 -4.20
Golgi 1.78 2.60 2.28 1.39 -4.81 -1.67 -0.08 -1.15 1.17 -1.02 -0.40 3.07 1.14 1.20 -2.79
Lipid Particles 3.75 5.48 4.40 0.76 -4.52 2.12 0.25 2.20 0.47 3.28 4.14 4.92 4.38 0.97 -4.41
Mitochondria 0.58 1.80 1.51 1.42 -1.38 -2.54 1.48 -2.26 0.70 -4.80 -1.93 2.01 -1.51 0.66 -4.33
Mitotic Spindle -1.33 -0.26 -1.60 -1.18 -1.59 -1.31 1.27 -5.55 -4.78 -6.48 -2.13 0.79 -6.15 -4.81 -6.59
None 2.84 6.39 5.72 1.68 -0.35 3.99 6.61 4.31 -0.24 -3.40 5.31 9.86 6.00 0.03 -4.88
Nuclear Periphery -0.52 -0.24 -2.46 -2.39 -2.90 -2.79 -0.72 -7.63 -4.24 -7.48 -2.50 -1.14 -7.32 -4.59 -7.45
Nuclear Periphery Foci 1.04 2.30 0.85 -0.27 -2.84 0.03 -1.43 -2.06 -2.03 0.63 1.36 2.56 -0.93 -1.98 -2.77
Nucleolus 3.39 10.55 11.02 5.35 2.68 4.90 1.59 7.66 5.05 1.89 6.98 12.39 11.25 6.06 -1.86
Nucleus -1.16 -7.22 1.01 1.54 3.35 -3.15 -0.00 7.15 10.65 3.94 -4.61 -8.27 6.36 10.49 13.93
Peroxisomes 2.72 3.77 3.55 3.82 -2.90 -0.38 -0.54 1.92 2.94 1.83 1.54 4.29 3.97 3.22 -2.07
Vacuole -1.89 -2.97 -3.84 -3.21 -3.57 -1.85 -3.06 -9.60 -8.96 0.19 -2.71 -4.03 -10.38 -9.31 -8.15
Vacuole Periphery -1.20 -0.24 0.33 1.22 0.37 -3.16 -0.49 -2.68 -1.44 -2.55 -2.74 -0.49 -2.59 -0.90 -1.95
Standard name
Human Ortholog
Description Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Arg80

Arg80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arg80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available