Standard name
Human Ortholog
Description Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.07 0 0.06 0.07 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0 0 0 0 0 0 0 0 0.14 0.16 0.2 0.05 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.23 0 0.16 0.2 0.14 0.38 0.33 0.35 0.38 0.4 0.36 0.44 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.88 0.9 0.9 0.78 0.86 0.77 0.73 0.77 0.72 0.72 0.64 0.64 0.81 0.81 0.77 0.9 0.89 0.88 0.77 0.79 0.71 0.68 0.69 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0 0.17 0.21 0.14 0.18 0.23 0.21 0.24 0.36 0.4 0.4 0.1 0.07 0.07 0.05 0.06 0.08 0.09 0.06 0.16 0.18 0.18 0.18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 3
Bud 0 0 0 0 0 1 2 2 1 1 0 2 0 0 1 1 0 2 2 5 0 2 5 7
Bud Neck 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 1 0 0 0 0 3 2
Bud Site 4 0 0 1 2 5 19 8 11 16 16 10 0 0 2 4 0 1 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 9 8 4 6 6 2 8 10 8 6 9 6 61 101 131 39 25 25 3 0 1 1 2 1
Endoplasmic Reticulum 1 2 0 0 0 0 1 1 0 0 0 0 1 0 1 7 5 5 1 0 0 0 1 4
Endosome 2 2 1 0 2 0 1 1 0 1 0 0 1 4 7 4 4 4 3 5 0 5 5 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1 1 3 0 0 2 1 1
Mitochondria 73 11 22 20 26 81 92 118 69 93 92 96 2 4 5 59 23 33 3 4 2 2 4 3
Nucleus 276 251 124 79 165 165 207 259 131 169 162 139 352 517 507 747 579 458 234 239 99 149 365 264
Nuclear Periphery 1 0 2 0 0 1 4 5 3 2 5 0 2 5 6 11 11 8 2 0 1 0 0 1
Nucleolus 56 12 24 21 27 39 66 69 43 85 101 86 43 47 47 39 41 40 26 18 22 39 93 73
Peroxisomes 0 1 0 0 0 0 0 0 1 0 0 0 0 2 2 1 0 1 0 0 0 1 0 1
SpindlePole 5 2 0 0 1 0 1 5 0 1 1 0 8 4 6 24 28 11 5 4 1 4 14 12
Vac/Vac Membrane 3 10 0 0 1 5 1 3 0 0 0 2 3 1 7 7 9 7 10 19 8 6 24 23
Unique Cell Count 313 280 138 101 192 213 282 335 182 234 255 216 433 636 658 828 649 522 305 303 141 221 532 418
Labelled Cell Count 431 301 177 127 230 299 402 481 267 375 388 341 473 686 723 948 726 597 305 303 141 221 532 418


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.0 5.1 5.5 5.4 5.0 4.7 4.7 5.1 4.2 4.7 4.8 6.6 5.9 5.9 7.0 8.3 7.9 6.0 5.9 6.8
Std Deviation (1e-4) 1.2 1.1 1.0 1.5 1.6 1.7 1.6 1.5 1.8 1.2 1.5 1.4 1.7 1.6 1.6 1.4 1.7 1.6 1.6 1.3 1.6
Intensity Change (Log2) – – – 0.09 0.07 -0.03 -0.12 -0.14 -0.01 -0.28 -0.12 -0.11 0.35 0.19 0.2 0.45 0.69 0.61 0.23 0.19 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.7 0 0 0 3.6 4.0 4.8 1.0 0.5 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.8 -0.6 4.4 0 0 4.3 0 0 0 -7.9 0 0 -3.5 -5.7 -3.6
Nucleus -2.5 -1.1 -3.0 -3.9 -3.2 -3.9 -4.0 -5.6 -5.4 -2.4 -2.4 -3.4 0.1 -0.2 -0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 -0.8 0.2 0 0 1.4 0 0 0 -2.4 -3.7 -3.8 -5.6 -4.3 -3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.238 1.3666 1.1559 1.7459 0.5563 1.146 5.441 4.4561 4.7683 4.9044 4.3456 4.7437 6.2125 5.6713 5.5503 5.9389 5.8402 5.6493
Actin 0.001 0 0.0011 0 0.0002 0.0003 0.0002 0 0.0009 0.0003 0 0 0.0003 0 0 0 0 0
Bud 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0 0.0062 0 0.0001 0 0
Bud Neck 0.0004 0.0001 0.0005 0 0.0006 0.0007 0.0004 0.0001 0.0003 0.0015 0.0003 0.0004 0.0002 0.0001 0 0.0001 0.0002 0.0004
Bud Periphery 0.0001 0 0.0001 0 0.0001 0.0005 0.0003 0 0.0001 0.0001 0.0001 0 0.0001 0.0005 0 0 0 0
Bud Site 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0001 0 0.0002 0.0006 0.0001 0 0 0.0004 0 0 0 0
Cell Periphery 0 0 0 0 0 0.0002 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0009 0.0006 0.0003 0 0.0017 0.0007 0.0001 0.0001 0.0006 0.0004 0 0 0.0033 0.0018 0 0.0031 0 0
Cytoplasmic Foci 0.0066 0 0.0005 0 0.0001 0.0005 0 0 0.0004 0.0004 0 0 0.0138 0.0001 0 0.0011 0 0.0001
Eisosomes 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0005 0.0001 0.0001 0 0.0003 0.0003 0.0001 0 0.0004 0.0002 0 0 0.0003 0 0 0 0 0
Endosome 0.006 0.0001 0.0014 0 0.0004 0.0022 0.0001 0 0.0018 0.0008 0 0 0.0009 0 0 0 0 0
Golgi 0.0011 0 0.0007 0 0 0.0006 0 0 0.0001 0.0002 0 0 0 0 0 0 0 0
Lipid Particles 0.0046 0.0001 0.0047 0 0.0001 0.0019 0 0 0.0006 0.0007 0 0 0.0002 0 0 0 0 0
Mitochondria 0.0012 0.0001 0.0026 0 0.0004 0.0067 0.0011 0 0.0003 0.0002 0.0003 0 0.0004 0.0003 0 0.0001 0.0001 0
None 0.0039 0.0006 0.0028 0 0.0029 0.0028 0.0001 0 0.0011 0.0003 0.0001 0 0.0027 0.0004 0 0.0003 0 0
Nuclear Periphery 0.0062 0.0024 0.0031 0.0001 0.0056 0.0036 0.001 0.0007 0.0019 0.0042 0.0017 0.0001 0.0025 0.0012 0.0002 0.0009 0.001 0.0002
Nucleolus 0.0284 0.0251 0.0186 0.015 0.0897 0.073 0.0208 0.0212 0.0194 0.0364 0.0879 0.0565 0.0142 0.0187 0.0135 0.0091 0.0828 0.0403
Nucleus 0.9046 0.967 0.9511 0.9847 0.8458 0.8932 0.9711 0.9711 0.969 0.9241 0.899 0.9404 0.9448 0.9647 0.9783 0.9679 0.9121 0.9437
Peroxisomes 0.0055 0 0.0015 0 0 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0272 0.0037 0.0101 0.0001 0.0511 0.01 0.0038 0.0068 0.002 0.0288 0.0103 0.0025 0.0159 0.0052 0.0079 0.0173 0.0034 0.015
Vacuole 0.001 0.0001 0.0002 0 0.0005 0.0012 0.0003 0 0.0006 0.0004 0.0001 0 0.0001 0.0002 0 0 0.0001 0
Vacuole Periphery 0.0006 0 0.0002 0 0.0002 0.0012 0.0003 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.7269 18.65 15.8893 19.3334 19.5357 21.6234 17.8094 17.9123 21.6528 15.4872
Translational Efficiency 4.3783 1.8171 1.9316 2.0566 1.834 1.6272 1.4969 1.6667 1.4537 2.2638

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
291 1506 2564 1377 779 1338 797 386 1070 2844 3361 1763

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.12 751.31 949.95 929.26 688.90 777.75 963.32 994.40 694.94 763.75 953.12 943.52
Standard Deviation 83.52 115.71 130.19 146.83 105.21 115.74 128.76 149.90 100.27 116.48 129.97 149.95
Intensity Change Log 2 — 0.079315 0.417759 0.385989 — 0.175012 0.483721 0.529532 — 0.127198 0.450593 0.458406

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000151 0.001099 0.000689 0.001028 0.000452 0.000762 0.001289 0.002524 0.000371 0.000940 0.000831 0.001356
Bud Neck 0.002804 0.017212 0.042335 0.035674 0.002549 0.014498 0.022065 0.031668 0.002619 0.015935 0.037528 0.034797
Bud Site 0.000532 0.002989 0.001798 0.005000 0.000811 0.002454 0.002354 0.009612 0.000735 0.002737 0.001930 0.006010
Cell Periphery 0.000064 0.000184 0.000060 0.000154 0.000086 0.000137 0.000078 0.000311 0.000080 0.000162 0.000065 0.000188
Cytoplasm 0.003679 0.010472 0.000938 0.003513 0.011810 0.005960 0.001474 0.006940 0.009598 0.008349 0.001065 0.004263
Cytoplasmic Foci 0.002247 0.002077 0.000009 0.000507 0.002934 0.004083 0.000231 0.009726 0.002747 0.003021 0.000062 0.002525
Eisosomes 0.000022 0.000035 0.000041 0.000043 0.000033 0.000041 0.000049 0.000057 0.000030 0.000038 0.000042 0.000046
Endoplasmic Reticulum 0.000979 0.005161 0.002572 0.003209 0.002254 0.003892 0.004419 0.002693 0.001907 0.004564 0.003010 0.003096
Endosome 0.000495 0.001099 0.000151 0.000740 0.000411 0.003199 0.001464 0.014078 0.000433 0.002087 0.000462 0.003660
Golgi 0.002509 0.000676 0.000131 0.000579 0.000203 0.001738 0.000365 0.007464 0.000831 0.001176 0.000186 0.002086
Lipid Particles 0.000460 0.000812 0.000031 0.000062 0.001109 0.002211 0.000122 0.000313 0.000932 0.001471 0.000052 0.000117
Mitochondria 0.005393 0.003774 0.001530 0.002222 0.001565 0.007961 0.001901 0.005422 0.002606 0.005744 0.001618 0.002922
Mitotic Spindle 0.001833 0.003826 0.002845 0.014942 0.000777 0.010093 0.012326 0.019739 0.001064 0.006775 0.005094 0.015992
None 0.009626 0.012543 0.002247 0.004847 0.025189 0.008995 0.004751 0.011960 0.020956 0.010874 0.002841 0.006404
Nuclear Periphery 0.000410 0.000763 0.000405 0.000641 0.000469 0.001396 0.001059 0.000587 0.000453 0.001061 0.000560 0.000629
Nuclear Periphery Foci 0.000232 0.001268 0.000103 0.000348 0.000727 0.000867 0.000581 0.000523 0.000593 0.001079 0.000216 0.000386
Nucleolus 0.035256 0.058208 0.047333 0.050560 0.055742 0.075219 0.058422 0.052801 0.050171 0.066211 0.049963 0.051051
Nucleus 0.928576* 0.874968* 0.895852* 0.873481* 0.891517* 0.849304* 0.882264* 0.812476* 0.901596* 0.862894* 0.892630* 0.860124*
Peroxisomes 0.003815 0.001412 0.000292 0.000634 0.000738 0.002682 0.000805 0.001768 0.001575 0.002009 0.000413 0.000882
Vacuole 0.000452 0.000873 0.000460 0.001360 0.000419 0.003216 0.002485 0.006706 0.000428 0.001975 0.000940 0.002530
Vacuole Periphery 0.000464 0.000549 0.000178 0.000459 0.000205 0.001291 0.001497 0.002633 0.000275 0.000898 0.000491 0.000935

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.46 -19.19 -21.27 -1.39 -7.37 -4.08 -13.47 -4.91 -4.02 -1.62 -9.30 -16.01 -11.23 -4.93 -4.14
Bud Neck -11.61 -31.76 -21.25 -13.28 0.73 -12.12 -17.01 -14.24 -9.63 -4.39 -17.40 -38.25 -26.06 -16.72 -1.18
Bud Site -3.08 -2.21 -5.32 -3.37 -5.13 -3.08 -7.04 -5.04 -4.07 -3.43 -4.89 -5.89 -8.24 -5.38 -6.08
Cell Periphery -4.24 0.66 -2.34 1.97 -4.07 -2.56 0.15 -2.15 -1.06 -2.27 -4.96 1.65 -3.07 0.82 -4.62
Cytoplasm -3.48 2.01 0.15 4.79 -3.88 2.71 4.53 2.99 0.67 -2.61 0.84 5.45 3.55 4.68 -4.55
Cytoplasmic Foci 0.77 2.24 2.05 3.24 -1.59 -1.00 3.87 0.30 1.02 -1.93 0.06 4.54 3.13 3.98 -2.40
Eisosomes -3.90 -6.63 -7.60 -5.41 -2.42 -3.08 -7.98 -5.96 -4.06 -1.24 -3.84 -8.06 -8.76 -5.68 -2.46
Endoplasmic Reticulum -16.14 -10.40 -13.33 6.52 -2.95 -5.12 -8.84 -6.10 -1.73 1.27 -11.97 -8.65 -9.84 3.75 -1.41
Endosome -1.92 1.64 -0.31 1.53 -1.77 -3.68 -0.98 -2.07 -0.73 -1.79 -4.22 0.61 -2.08 0.93 -2.24
Golgi 0.81 1.05 0.90 0.73 -1.74 -3.29 -1.50 -1.71 -0.61 -1.61 -0.55 1.05 -0.18 0.53 -2.17
Lipid Particles -1.56 4.34 3.96 4.21 -3.78 -1.66 4.44 3.96 3.47 -1.74 -1.36 5.50 5.21 4.82 -3.27
Mitochondria 0.48 1.07 0.91 2.10 -2.90 -4.04 -1.87 -3.57 2.44 -2.85 -2.60 0.66 -0.16 3.67 -4.02
Mitotic Spindle -1.44 -2.26 -5.84 -5.44 -4.95 -5.23 -5.23 -4.62 -2.36 -0.60 -5.63 -6.48 -7.88 -5.12 -4.50
None -0.89 3.47 2.41 6.44 -4.50 5.00 6.96 5.04 0.55 -1.94 4.26 8.12 6.83 5.52 -4.50
Nuclear Periphery -2.25 -0.93 -2.77 -0.84 -3.22 -3.73 -2.52 -4.54 1.51 -0.06 -4.46 -2.20 -4.87 1.53 -2.46
Nuclear Periphery Foci -1.09 1.22 0.20 1.98 -1.88 -0.72 1.24 2.03 3.05 0.79 -1.50 3.16 0.95 2.44 -1.63
Nucleolus -4.22 -3.23 -3.85 0.28 -1.30 -4.97 -0.51 -0.55 2.67 -0.18 -5.16 -1.05 -1.92 3.05 -1.19
Nucleus 4.65 4.22 6.72 3.62 5.49 5.44 3.03 6.01 2.41 3.99 6.23 3.86 8.25 3.06 6.68
Peroxisomes 0.69 1.14 1.03 2.27 -5.50 -4.11 -0.67 -1.19 3.03 -0.91 -0.85 1.38 0.98 4.37 -3.66
Vacuole -1.65 -1.08 -3.35 -2.62 -3.17 -3.68 -5.60 -4.00 -2.54 -2.66 -4.14 -5.20 -5.35 -2.12 -3.74
Vacuole Periphery -0.31 0.93 0.26 1.34 -1.75 -4.16 -1.48 -1.90 0.33 -0.36 -4.15 -0.61 -1.71 1.94 -1.07
Standard name
Human Ortholog
Description Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ioc4

Ioc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ioc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available