Standard name
Human Ortholog
Description Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0 0 0.07 0.1 0.07 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0.05 0.08 0.09 0.09 0.09 0.07 0.11 0.07 0.07 0.1 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.05 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.82 0.75 0.7 0.71 0.64 0.61 0.52 0.45 0.4 0.33 0.35 0.86 0.91 0.86 0.12 0.15 0.15 0.58 0.53 0.52 0.78 0.87 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.11 0.12 0.07 0.05 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0.06 0.32 0.17 0.28 0.22 0 0 0 0.05 0 0 0.07 0.09 0.08 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.06 0 0 0 0 0 0
SpindlePole 0.1 0.1 0.22 0.23 0.24 0.28 0.35 0.42 0.34 0.45 0.44 0.47 0.1 0.06 0.1 0.83 0.76 0.76 0.22 0.22 0.28 0.05 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 4 4 2 4 1 1 3 0 2 4 2 1 0 0 1 1 5 10 3 2 1 1
Bud 4 13 4 7 4 12 13 23 29 23 47 27 1 0 0 2 6 7 2 7 2 1 4 5
Bud Neck 11 26 22 15 7 26 20 39 22 24 37 31 9 6 5 6 4 2 2 4 1 1 0 1
Bud Site 0 1 0 1 1 6 3 6 11 11 14 24 0 1 0 9 0 9 – – – – – –
Cell Periphery 0 5 1 0 0 1 0 0 0 0 1 1 1 1 0 0 0 0 0 3 1 0 1 2
Cytoplasm 222 399 201 112 58 192 181 178 135 132 120 126 240 250 280 28 35 26 186 272 142 128 214 191
Endoplasmic Reticulum 3 3 2 0 0 0 0 0 1 0 0 0 0 1 1 2 3 1 0 0 0 0 1 2
Endosome 22 6 1 0 0 3 1 2 2 4 4 0 2 7 20 20 25 20 21 24 14 2 2 2
Golgi 1 0 0 0 0 0 1 0 0 0 0 0 1 1 0 0 1 0 3 3 1 0 2 0
Mitochondria 10 17 0 4 1 27 13 22 94 56 104 79 0 1 1 12 6 5 21 45 22 7 7 5
Nucleus 0 3 0 0 0 1 1 3 1 2 3 4 0 0 0 0 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 4 2 0 0 0 9 4 10 0 0 0 1 0 0
Peroxisomes 0 1 0 0 0 2 1 3 7 3 5 2 0 1 14 25 14 10 0 3 1 0 0 0
SpindlePole 28 50 59 37 20 86 105 143 101 148 161 167 28 16 33 189 171 128 71 112 74 8 8 8
Vac/Vac Membrane 1 2 0 0 0 2 1 1 0 3 1 1 0 1 1 1 4 6 2 3 1 1 0 2
Unique Cell Count 278 488 268 160 82 302 297 340 297 332 366 359 278 275 327 227 226 168 324 512 271 164 247 229
Labelled Cell Count 303 526 294 181 93 362 342 421 407 407 503 468 284 287 355 303 275 228 324 512 271 164 247 229


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.3 4.6 4.1 3.7 3.8 3.9 4.3 3.1 3.5 3.3 3.4 4.7 4.7 4.4 5.0 6.0 6.1 4.7 5.2 5.4
Std Deviation (1e-4) 0.8 0.8 1.0 1.0 0.7 1.1 1.2 1.7 0.8 0.8 1.1 1.0 0.9 1.4 1.1 1.0 1.3 1.3 1.6 1.8 1.8
Intensity Change (Log2) – – – -0.17 -0.32 -0.28 -0.23 -0.09 -0.59 -0.38 -0.48 -0.45 0.04 0.04 -0.07 0.12 0.38 0.41 0.03 0.19 0.24

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000246WT3HU80HU120HU1600246WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30246WT1AF100AF140AF1800246
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.365 3.4305 2.8073 2.5646 2.3889 2.7469 3.9915 4.0444 3.9821 3.6012 3.7444 4.1481 1.5943 1.4675 1.4139 1.6457 1.3799 1.5464
Actin 0.0167 0.0003 0.0203 0.0142 0.0047 0.0096 0.0296 0.0075 0.0028 0.0007 0.0015 0.0034 0.0437 0.0004 0.0012 0.0088 0.0007 0.0058
Bud 0.0002 0.0001 0.0001 0.0002 0.0001 0.0005 0.0025 0.0025 0.0006 0 0.0002 0.0005 0.0002 0.0003 0.0003 0.0001 0 0.0002
Bud Neck 0.0027 0.0008 0.0077 0.0948 0.0003 0.0031 0.0028 0.0015 0.0027 0.0108 0.0006 0.0027 0.0024 0.0003 0.005 0.0887 0.0004 0.001
Bud Periphery 0.0002 0.0001 0.0001 0.0004 0.0003 0.0028 0.004 0.0041 0.0007 0 0.0003 0.0008 0.0004 0.0008 0.0004 0.0001 0.0001 0.0013
Bud Site 0.0036 0.0013 0.0021 0.0076 0.0005 0.0003 0.0102 0.017 0.0043 0 0.0008 0.0005 0.0024 0.0025 0.0025 0.0007 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0.0006 0.0001 0.0002 0.0004 0.0003 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.0194 0.041 0.0232 0.0196 0.0245 0.0407 0.0333 0.0616 0.0172 0.0092 0.0031 0.0343 0.0288 0.0298 0.0213 0.0158 0.0075 0.032
Cytoplasmic Foci 0.3096 0.2612 0.3327 0.523 0.384 0.3478 0.2868 0.2029 0.3083 0.3158 0.6108 0.2918 0.2691 0.2002 0.2408 0.5103 0.4138 0.2228
Eisosomes 0.0004 0.0006 0.0005 0.0014 0.0003 0.0001 0.0007 0.0002 0.0003 0.0003 0 0.0001 0.0008 0.0002 0.0002 0.0008 0.0001 0.0001
Endoplasmic Reticulum 0.0007 0.0005 0.001 0.0016 0.0007 0.0014 0.0018 0.0019 0.0018 0.0002 0.001 0.0007 0.0022 0.0007 0.0006 0.0002 0.0003 0.0007
Endosome 0.0145 0.0145 0.0097 0.0065 0.0581 0.0168 0.0067 0.0131 0.0216 0.0012 0.0134 0.0102 0.0167 0.0109 0.0129 0.0072 0.0184 0.0073
Golgi 0.0052 0.0012 0.0026 0.0042 0.0066 0.0046 0.0031 0.0038 0.0022 0.0007 0.0041 0.0011 0.0043 0.0011 0.0013 0.0014 0.0042 0.0019
Lipid Particles 0.0438 0.0175 0.0236 0.0157 0.0916 0.0125 0.0163 0.0068 0.0077 0.0126 0.042 0.0138 0.0202 0.0078 0.0145 0.0088 0.0993 0.013
Mitochondria 0.0009 0.0002 0.0016 0.0024 0.003 0.0013 0.0015 0.0019 0.0006 0.0001 0.0003 0.0004 0.001 0.0004 0.0005 0.0003 0.0003 0.0018
None 0.3884 0.5486 0.4244 0.1101 0.3242 0.4066 0.4572 0.5614 0.4949 0.5281 0.1406 0.5093 0.5192 0.6745 0.5458 0.1637 0.1364 0.5418
Nuclear Periphery 0.0056 0.0005 0.0007 0.0011 0.0085 0.0015 0.0215 0.001 0.0016 0.0002 0.0033 0.0013 0.0053 0.0008 0.0009 0.0007 0.0014 0.0022
Nucleolus 0.0007 0.0138 0.0008 0.001 0.0008 0.0004 0.0011 0.0012 0.0011 0.0002 0.0006 0.0004 0.0009 0.001 0.0016 0.0014 0.0007 0.0005
Nucleus 0.0005 0.0073 0.0007 0.0011 0.001 0.0011 0.0109 0.0014 0.001 0.0002 0.0007 0.0005 0.0016 0.001 0.0016 0.0009 0.0005 0.001
Peroxisomes 0.056 0.0172 0.0325 0.0597 0.0629 0.0162 0.0221 0.0205 0.0141 0.0171 0.067 0.0111 0.0261 0.0151 0.018 0.0128 0.0673 0.0196
Punctate Nuclear 0.13 0.0717 0.1147 0.134 0.0255 0.1312 0.086 0.0876 0.1153 0.1023 0.1091 0.1165 0.0537 0.0509 0.1295 0.1771 0.2482 0.1462
Vacuole 0.0005 0.0013 0.0006 0.0005 0.0015 0.0008 0.0009 0.0015 0.0007 0 0.0005 0.0004 0.0007 0.0009 0.0008 0.0003 0.0003 0.0003
Vacuole Periphery 0.0002 0.0001 0.0001 0.0002 0.0006 0.0004 0.0005 0.0005 0.0002 0 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1883 11.7808 3.1789 6.781 10.0966 5.9869 12.9944 6.5778 4.3873 10.5096
Translational Efficiency 0.9823 0.5252 1.7342 0.6471 0.6058 1.0932 0.5841 0.9525 1.2995 0.7618

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1603 1764 214 114 2189 1432 1031 1886 3792 3196 1245 2000

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 689.35 707.54 842.06 904.90 640.01 771.91 807.90 843.37 660.87 736.38 813.77 846.88
Standard Deviation 78.04 82.43 92.72 103.14 72.70 91.08 96.07 112.96 78.86 92.15 96.36 113.33
Intensity Change Log 2 — 0.037575 0.288686 0.392522 — 0.270338 0.336082 0.398071 — 0.154329 0.311699 0.395196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000148 0.000643 0.000893 0.000485 0.000089 0.001045 0.000488 0.000668 0.000114 0.000823 0.000557 0.000657
Bud Neck 0.012197 0.031853 0.006033 0.003074 0.016808 0.051278 0.009217 0.013054 0.014858 0.040557 0.008670 0.012485
Bud Site 0.003730 0.014601 0.012048 0.024918 0.004254 0.021881 0.030729 0.055250 0.004033 0.017863 0.027518 0.053521
Cell Periphery 0.000110 0.000117 0.000263 0.000125 0.000133 0.000218 0.000201 0.000117 0.000123 0.000162 0.000211 0.000117
Cytoplasm 0.244627 0.293784 0.117167 0.069548 0.338951 0.289064 0.115737 0.101233 0.299077 0.291669 0.115983 0.099427
Cytoplasmic Foci 0.525100 0.394657 0.098229 0.124113 0.414650 0.387870 0.108094 0.129072 0.461341 0.391616 0.106398 0.128789
Eisosomes 0.000156 0.000170 0.000174 0.000045 0.000186 0.000268 0.000061 0.000032 0.000173 0.000214 0.000081 0.000033
Endoplasmic Reticulum 0.000525 0.000792 0.005053 0.000927 0.001006 0.000415 0.000951 0.000425 0.000803 0.000623 0.001656 0.000454
Endosome 0.024563 0.032431 0.021770 0.014555 0.017745 0.030826 0.016064 0.022047 0.020627 0.031712 0.017045 0.021620
Golgi 0.008309 0.015998 0.001078 0.002301 0.006230 0.014056 0.003719 0.003976 0.007109 0.015128 0.003265 0.003880
Lipid Particles 0.038279 0.014703 0.014954 0.008075 0.036614 0.017348 0.005690 0.004336 0.037318 0.015888 0.007283 0.004549
Mitochondria 0.005092 0.021352 0.000549 0.000906 0.005789 0.021784 0.000794 0.002156 0.005494 0.021546 0.000752 0.002085
Mitotic Spindle 0.017128 0.023476 0.228816 0.600079 0.006054 0.009835 0.525438 0.576527 0.010735 0.017364 0.474452 0.577870
None 0.006448 0.003191 0.004660 0.000453 0.007872 0.004250 0.008368 0.001859 0.007270 0.003665 0.007731 0.001779
Nuclear Periphery 0.000901 0.000406 0.001928 0.000758 0.000660 0.000329 0.000890 0.000517 0.000762 0.000372 0.001069 0.000530
Nuclear Periphery Foci 0.003543 0.001405 0.050772 0.054567 0.001658 0.000741 0.031512 0.007518 0.002455 0.001107 0.034822 0.010200
Nucleolus 0.004365 0.002835 0.014689 0.010366 0.003794 0.002823 0.013237 0.005711 0.004035 0.002830 0.013487 0.005977
Nucleus 0.051205 0.061507 0.317178 0.025159 0.100084 0.064284 0.077299 0.014434 0.079421 0.062751 0.118531 0.015045
Peroxisomes 0.038328 0.041059 0.026357 0.027352 0.019299 0.041530 0.033710 0.038957 0.027343 0.041270 0.032447 0.038296
Vacuole 0.014711 0.042921 0.076886 0.031271 0.017444 0.038231 0.017397 0.020310 0.016288 0.040819 0.027622 0.020935
Vacuole Periphery 0.000534 0.002101 0.000502 0.000923 0.000682 0.001925 0.000403 0.001802 0.000620 0.002022 0.000420 0.001752

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.66 -7.16 -5.31 -1.69 1.47 -2.29 -11.09 -7.83 0.84 -1.20 -3.69 -12.72 -8.14 0.58 -0.05
Bud Neck -10.64 -0.39 5.21 13.37 3.05 -11.26 5.57 3.87 13.04 -1.93 -14.81 4.29 2.84 15.90 -1.37
Bud Site -12.32 -6.20 -5.16 -3.88 -2.76 -8.24 -11.31 -18.15 -8.75 -5.77 -12.75 -12.75 -19.12 -12.34 -6.68
Cell Periphery -0.95 -6.55 -1.01 -0.84 2.13 -3.92 -2.22 2.38 4.95 3.79 -3.64 -4.78 1.33 4.06 5.07
Cytoplasm -7.67 13.37 7.35 11.26 -0.68 3.64 31.55 31.02 23.29 -2.35 -1.62 32.19 30.13 30.31 -2.55
Cytoplasmic Foci 15.94 31.91 23.27 15.66 0.49 4.38 35.53 38.23 29.60 -1.22 12.97 49.56 52.29 39.19 -1.33
Eisosomes -1.26 -5.00 2.12 2.50 5.51 -4.97 8.68 16.74 14.30 10.64 -3.40 5.21 19.78 12.38 13.34
Endoplasmic Reticulum -3.44 -7.59 -3.54 -2.72 5.05 2.77 -2.81 2.20 -1.76 8.52 0.99 -8.02 1.14 0.32 10.11
Endosome -4.59 -2.33 1.17 3.46 2.60 -6.53 -4.15 -0.81 5.46 3.21 -8.32 -3.97 0.72 7.53 4.02
Golgi -6.30 7.28 6.06 10.76 -0.01 -5.31 3.34 4.11 7.58 0.43 -8.48 5.86 6.72 12.91 0.25
Lipid Particles 9.45 9.13 12.87 5.91 4.29 8.04 14.95 16.18 9.14 2.92 12.55 18.99 21.44 12.16 4.70
Mitochondria -9.37 8.34 8.12 12.07 1.40 -8.09 8.46 6.87 10.79 -2.51 -12.17 11.51 8.74 15.73 -2.37
Mitotic Spindle -1.09 -9.73 -12.36 -12.27 -5.45 -2.15 -39.01 -62.32 -61.08 -4.58 -3.14 -38.40 -62.49 -61.37 -7.53
None 5.78 3.73 6.32 2.55 3.45 3.35 2.07 6.69 3.46 3.78 6.26 2.77 8.93 3.90 4.22
Nuclear Periphery 0.89 -3.39 -0.57 -3.45 4.67 2.48 -3.96 -0.39 -5.67 5.53 1.71 -3.46 -0.01 -6.22 7.77
Nuclear Periphery Foci 4.95 -7.85 -3.62 -3.99 2.36 3.10 -14.56 -8.83 -10.04 10.48 5.36 -16.25 -8.63 -10.39 11.59
Nucleolus 4.19 -6.53 0.25 -1.02 6.10 3.71 -9.41 -3.85 -5.96 7.47 5.24 -11.01 -2.96 -5.63 9.23
Nucleus -4.99 -7.79 1.93 3.96 8.03 7.48 7.38 24.21 14.31 7.53 3.95 -1.02 22.92 18.40 10.98
Peroxisomes -1.12 -0.68 0.18 0.58 0.61 -8.66 -4.39 -7.52 1.71 -1.66 -7.70 -2.32 -4.23 1.99 -1.09
Vacuole -13.80 -6.31 -4.47 -2.68 -1.30 -9.61 -1.64 -4.23 4.08 -1.67 -16.45 -5.28 -6.45 4.19 -0.41
Vacuole Periphery -8.92 -0.25 0.09 5.30 0.16 -5.76 5.75 -1.18 2.95 -2.77 -10.15 5.72 -1.48 3.96 -2.65
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Bub2

Bub2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bub2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available