Standard name
Human Ortholog
Description Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud – 0.07 – 0 0 0.07 0.05 0.06 0 0 0.07 0.05 0.06 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0 0 0 0 0
Bud Site – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm – 0.26 – 0.36 0.45 0.58 0.44 0.45 0.44 0.44 0.43 0.38 0.46 0.74 0.69 – – – 0.18 0.09 0.16 0.09 0.1 0.11
Endoplasmic Reticulum – 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome – 0.21 – 0 0 0 0 0 0 0 0 0 0 0.06 0.06 – – – 0.12 0.17 0.16 0.08 0.08 0.09
Golgi – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0.33 – 0.59 0.56 0.46 0.64 0.58 0.65 0.59 0.66 0.71 0.46 0.05 0 – – – 0.15 0.46 0.42 0.51 0.47 0.43
Nucleus – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0 0 0 0 0
Nuclear Periphery – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.11 0 0 0 0 0
Peroxisomes – 0.07 – 0.06 0 0 0 0 0 0 0 0 0 0 0.05 – – – 0 0 0 0 0 0
SpindlePole – 0.07 – 0.09 0.07 0 0.05 0.11 0.06 0.1 0.09 0.08 0.14 0.11 0.08 – – – 0.11 0.08 0 0 0.05 0.05
Vac/Vac Membrane – 0.15 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.07 0.05 0.06 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 1 – 1 0 3 0 0 0 0 0 0 0 0 2 – – – 0 2 1 3 5 6
Bud – 4 – 5 9 13 17 24 11 11 11 14 2 1 2 – – – 1 1 0 2 4 7
Bud Neck – 0 – 0 3 1 3 7 3 9 3 4 0 5 5 – – – 1 2 0 2 6 7
Bud Site – 0 – 0 0 4 2 8 10 5 5 8 1 0 1 – – – – – – – – –
Cell Periphery – 1 – 0 1 1 0 0 0 0 1 1 0 1 1 – – – 0 0 0 0 0 0
Cytoplasm – 16 – 49 108 105 164 182 120 119 68 114 16 109 119 – – – 5 8 8 7 18 19
Endoplasmic Reticulum – 3 – 3 1 2 0 1 0 0 0 0 0 3 7 – – – 0 0 0 2 2 0
Endosome – 13 – 2 2 1 1 2 2 1 2 0 1 9 11 – – – 3 16 8 6 15 15
Golgi – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Mitochondria – 20 – 81 136 83 240 236 178 158 105 214 16 8 4 – – – 4 42 22 40 91 75
Nucleus – 0 – 1 1 2 4 4 3 3 3 3 1 0 4 – – – 1 0 0 0 1 2
Nuclear Periphery – 1 – 0 0 0 0 1 1 0 0 0 0 3 0 – – – 0 0 1 1 3 0
Nucleolus – 1 – 2 3 0 0 3 1 1 2 0 1 4 2 – – – 3 0 0 1 2 0
Peroxisomes – 4 – 8 0 1 3 3 2 0 0 2 0 4 8 – – – 1 0 0 2 3 3
SpindlePole – 4 – 13 18 8 17 43 17 27 14 23 5 16 14 – – – 3 7 2 3 9 8
Vac/Vac Membrane – 9 – 2 1 0 4 0 4 0 2 0 0 3 7 – – – 0 6 2 4 18 22
Unique Cell Count 61 137 242 180 375 406 275 269 158 302 35 148 173 30 93 54 81 192 175
Labelled Cell Count 77 167 283 224 455 514 352 334 216 383 43 166 187 30 93 54 81 192 175


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 4.4 – 3.3 3.2 3.2 2.7 2.9 2.6 2.7 2.8 2.6 4.5 4.8 4.8 – – – 4.5 5.6 5.6
Std Deviation (1e-4) – 0.8 – 1.2 1.2 1.6 1.6 1.5 1.4 1.6 2.1 1.5 1.1 1.1 1.2 – – – 0.7 2.0 2.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4673 -0.2855 -0.2645 -0.1343 -0.0856 -0.2401 -0.224 0.6402 0.5007 -0.7088 -0.4283 0.2394 -2.1524 -1.1311 -1.4339 -1.7517 -1.3 -1.4605
Actin 0.0109 0.0011 0.0041 0.0181 0.0018 0.0044 0.0323 0.0006 0.0012 0.0013 0.005 0.0025 0.0338 0.0093 0.0196 0.0006 0.0927 0.0106
Bud 0.0056 0.003 0.0074 0.0148 0.0055 0.0039 0.0003 0.0047 0.0054 0.0004 0.0175 0.001 0.0037 0.012 0.0041 0.0056 0.0153 0.0012
Bud Neck 0.002 0.0052 0.0029 0.0112 0.0038 0.0114 0.0012 0.0027 0.0095 0.0002 0.0014 0.0517 0.0021 0.0054 0.0039 0.0013 0.001 0.0164
Bud Periphery 0.0044 0.004 0.0048 0.0224 0.011 0.006 0.0007 0.0108 0.0112 0.0016 0.0621 0.0045 0.0092 0.0203 0.0087 0.0059 0.0721 0.0019
Bud Site 0.0087 0.0293 0.0158 0.0508 0.0854 0.0022 0.0011 0.0168 0.0187 0.0008 0.0034 0.0016 0.0093 0.0485 0.0164 0.006 0.0029 0.0028
Cell Periphery 0.001 0.0014 0.0014 0.0023 0.0021 0.0009 0.002 0.0017 0.0011 0.0009 0.002 0.001 0.0018 0.0026 0.0017 0.0004 0.0011 0.0014
Cytoplasm 0.0737 0.0726 0.1002 0.0384 0.0275 0.0699 0.214 0.0398 0.0767 0.0435 0.0183 0.1092 0.0682 0.0812 0.0939 0.0989 0.1203 0.0675
Cytoplasmic Foci 0.1438 0.151 0.1256 0.1565 0.2328 0.223 0.117 0.1094 0.0568 0.1058 0.0689 0.1686 0.1391 0.1501 0.2018 0.1418 0.1448 0.1203
Eisosomes 0.0008 0.0005 0.0004 0.0006 0.0006 0.0003 0.0007 0.0004 0.0004 0.0014 0.0007 0.0002 0.0016 0.0004 0.0004 0.0001 0.0017 0.0003
Endoplasmic Reticulum 0.003 0.0035 0.0065 0.0032 0.0031 0.002 0.0214 0.0028 0.0036 0.0057 0.0073 0.002 0.0041 0.0043 0.0049 0.0025 0.0048 0.0025
Endosome 0.1106 0.0955 0.1403 0.1379 0.0243 0.1248 0.1281 0.0866 0.0231 0.1007 0.0162 0.0963 0.1287 0.1325 0.1776 0.1798 0.1501 0.207
Golgi 0.0203 0.0072 0.0228 0.0176 0.0046 0.0117 0.0249 0.0054 0.0027 0.013 0.002 0.0184 0.0076 0.0183 0.0155 0.0286 0.0218 0.0321
Lipid Particles 0.0436 0.0472 0.0314 0.0651 0.0322 0.0388 0.0316 0.0437 0.0203 0.0101 0.0288 0.0758 0.034 0.0414 0.0341 0.0258 0.0307 0.0499
Mitochondria 0.0191 0.0254 0.0601 0.037 0.0445 0.0433 0.0038 0.0121 0.0089 0.0458 0.0295 0.1069 0.0263 0.0318 0.0853 0.0446 0.1091 0.0801
None 0.4656 0.418 0.375 0.2535 0.4413 0.3229 0.3856 0.5612 0.6576 0.6508 0.5772 0.3028 0.4267 0.3049 0.2015 0.2927 0.1793 0.2542
Nuclear Periphery 0.0072 0.0027 0.0054 0.0031 0.0025 0.0067 0.0025 0.0019 0.0096 0.0028 0.0293 0.001 0.0029 0.0017 0.0047 0.0037 0.0021 0.0031
Nucleolus 0.0023 0.0313 0.0067 0.0205 0.0119 0.007 0.0003 0.005 0.0061 0.0007 0.0207 0.0013 0.005 0.0208 0.0067 0.0026 0.0016 0.0111
Nucleus 0.014 0.0134 0.0124 0.0108 0.0046 0.0095 0.0013 0.0037 0.0461 0.0013 0.0485 0.0014 0.0027 0.0066 0.007 0.0036 0.0015 0.0055
Peroxisomes 0.0209 0.0327 0.0162 0.0732 0.0219 0.0539 0.0199 0.0078 0.0144 0.0046 0.0078 0.0292 0.0323 0.0216 0.0293 0.0333 0.0183 0.0104
Punctate Nuclear 0.0255 0.0127 0.0073 0.01 0.019 0.0112 0.0026 0.0401 0.0082 0.0022 0.0105 0.0014 0.019 0.0037 0.006 0.0415 0.0016 0.0059
Vacuole 0.0141 0.0389 0.0482 0.0466 0.0165 0.0402 0.0076 0.0385 0.0167 0.0046 0.0281 0.0199 0.0363 0.0788 0.07 0.0695 0.0202 0.109
Vacuole Periphery 0.0027 0.0032 0.0052 0.0065 0.003 0.0059 0.0012 0.0043 0.0015 0.0017 0.015 0.0032 0.0056 0.0038 0.0067 0.011 0.0069 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.0921 7.0169 12.6452 17.1263 12.3579 7.8451 19.5107 20.5221 21.0677 21.4619
Translational Efficiency 2.1236 2.8309 1.3173 0.9936 1.4491 3.0161 1.2837 0.8894 1.145 1.1295

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
170 931 1262 1032 1127 408 2321 1310 1297 1339 3583 2342

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.81 681.63 808.57 793.86 635.08 733.44 783.71 845.05 636.49 697.42 792.47 822.49
Standard Deviation 82.11 104.33 114.10 129.26 89.94 101.02 107.08 131.87 89.02 106.05 110.24 133.18
Intensity Change Log 2 — 0.077879 0.324263 0.297775 — 0.207741 0.303382 0.412098 — 0.143727 0.313947 0.355590

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000213 0.002090 0.002004 0.010905 0.001183 0.001022 0.003886 0.010008 0.001056 0.001765 0.003223 0.010403
Bud Neck 0.093117 0.100711 0.032639 0.073980 0.068937 0.116450 0.122235 0.107162 0.072106 0.105507 0.090678 0.092541
Bud Site 0.012041 0.059961 0.022299 0.162802* 0.016371 0.031038 0.097156 0.188531* 0.015803 0.051148 0.070790 0.177194*
Cell Periphery 0.000440 0.000426 0.000241 0.000540 0.000376 0.000417 0.000269 0.000451 0.000384 0.000423 0.000259 0.000490
Cytoplasm 0.158707* 0.163083* 0.183225* 0.117619* 0.123762* 0.121841* 0.200113* 0.120875* 0.128343* 0.150516* 0.194165* 0.119440*
Cytoplasmic Foci 0.221122* 0.171685 0.046767 0.085183 0.248107* 0.156097 0.068462 0.074424 0.244570* 0.166936 0.060821 0.079165
Eisosomes 0.000306 0.000222 0.000098 0.000226 0.000332 0.000250 0.000116 0.000167 0.000329 0.000231 0.000110 0.000193
Endoplasmic Reticulum 0.001250 0.001873 0.012782 0.005046 0.002198 0.001888 0.004519 0.002548 0.002073 0.001878 0.007430 0.003649
Endosome 0.080359 0.077915 0.018289 0.055798 0.109065 0.087025 0.032527 0.047238 0.105302 0.080691 0.027512 0.051010
Golgi 0.009626 0.018288 0.001942 0.019277 0.023513 0.009396 0.006394 0.019238 0.021693 0.015578 0.004826 0.019255
Lipid Particles 0.042802 0.014981 0.006988 0.006186 0.061941 0.023791 0.004402 0.003132 0.059433 0.017665 0.005313 0.004478
Mitochondria 0.071367 0.067495 0.006665 0.052936 0.087857 0.056522 0.039058 0.053349 0.085695 0.064151 0.027649 0.053167
Mitotic Spindle 0.008602 0.016048 0.047771 0.069149 0.005453 0.015762 0.052307 0.055237 0.005866 0.015961 0.050709 0.061367
None 0.009612 0.008304 0.005188 0.004003 0.008740 0.007773 0.002444 0.002136 0.008854 0.008142 0.003411 0.002959
Nuclear Periphery 0.001823 0.000936 0.005861 0.001679 0.002536 0.001583 0.002023 0.000841 0.002442 0.001133 0.003375 0.001210
Nuclear Periphery Foci 0.000810 0.000998 0.004087 0.001980 0.001687 0.001278 0.000898 0.000650 0.001572 0.001083 0.002021 0.001236
Nucleolus 0.014486 0.011763 0.014261 0.007372 0.011908 0.023496 0.006064 0.003314 0.012246 0.015338 0.008951 0.005102
Nucleus 0.139847* 0.124067 0.483005* 0.086617 0.098410 0.192784* 0.173030* 0.068116 0.103842 0.145005* 0.282209* 0.076268
Peroxisomes 0.049995 0.055487 0.011767 0.048634 0.062955 0.047557 0.036860 0.069204 0.061256 0.053070 0.028022 0.060140
Vacuole 0.073399 0.093478 0.090966 0.176884 0.057523 0.096947 0.142859 0.165535 0.059604 0.094535 0.124581 0.170536
Vacuole Periphery 0.010076 0.010190 0.003156 0.013182 0.007147 0.007083 0.004376 0.007843 0.007531 0.009243 0.003946 0.010196

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.01 -7.23 -6.29 -4.92 -4.86 0.25 -4.36 -5.38 -5.34 -3.71 -1.37 -4.77 -7.66 -7.03 -5.83
Bud Neck -0.35 3.85 1.32 3.71 -8.11 -3.76 -8.63 -5.34 0.71 2.63 -4.56 -3.47 -3.29 2.10 -0.03
Bud Site -8.26 -2.17 -17.56 -11.90 -18.33 -3.27 -19.96 -25.51 -20.42 -12.64 -10.22 -18.25 -31.19 -21.44 -19.65
Cell Periphery 0.33 3.12 -0.71 -1.18 -5.39 -0.55 2.28 -0.72 -0.12 -4.17 -0.53 2.87 -1.31 -0.80 -6.67
Cytoplasm -0.50 -0.71 2.05 4.86 5.89 0.05 -10.95 -0.66 -0.53 10.33 -3.27 -9.88 0.38 3.99 12.13
Cytoplasmic Foci 2.86 11.69 9.14 13.19 -8.17 7.92 27.68 25.43 9.70 -1.33 9.59 31.22 27.26 17.09 -5.78
Eisosomes 2.39 6.08 2.17 0.14 -4.09 2.27 7.27 5.29 4.77 -3.51 3.35 8.25 4.84 2.54 -5.28
Endoplasmic Reticulum -1.56 -18.01 -8.03 -8.16 11.80 0.46 -4.97 -1.46 -3.22 6.59 0.31 -12.09 -4.30 -8.42 11.94
Endosome 0.30 6.12 2.74 5.13 -11.30 2.64 15.07 11.80 5.28 -4.73 4.45 17.00 11.92 7.87 -10.11
Golgi -3.10 3.92 -2.51 0.61 -8.27 5.63 8.84 2.63 -3.29 -6.42 3.05 9.96 2.44 -0.82 -9.80
Lipid Particles 5.05 6.76 6.82 5.23 0.43 8.63 16.43 16.66 8.85 1.97 12.21 17.33 17.44 9.92 1.05
Mitochondria 0.40 5.09 1.90 3.38 -10.70 3.84 9.49 6.37 0.84 -3.20 3.80 12.11 7.07 3.34 -7.82
Mitotic Spindle -1.43 -6.57 -8.39 -9.03 -2.41 -2.41 -15.50 -12.10 -7.12 -0.50 -4.31 -17.49 -16.28 -11.83 -2.08
None 0.21 1.14 1.30 3.03 0.77 0.51 4.45 4.36 3.09 0.26 0.31 4.15 4.12 4.71 0.45
Nuclear Periphery 1.91 -9.10 -0.51 -4.57 11.92 2.02 0.06 2.91 1.55 5.36 3.40 -3.99 2.13 -2.86 11.39
Nuclear Periphery Foci -0.21 -6.66 -2.70 -3.26 4.05 0.61 2.37 2.65 1.98 0.80 1.42 -3.28 -0.25 -1.79 3.13
Nucleolus 0.50 0.32 1.85 3.12 4.23 -2.83 4.30 6.59 5.01 4.11 -1.80 2.71 5.83 6.38 5.30
Nucleus 0.74 -18.24 2.42 3.10 34.84 -7.11 -11.49 2.40 8.54 14.66 -5.42 -24.99 2.34 8.11 31.09
Peroxisomes -0.57 4.74 0.74 2.41 -9.50 2.59 6.96 0.08 -2.44 -6.43 1.90 9.76 1.64 -0.35 -9.54
Vacuole -1.83 -3.17 -10.78 -12.87 -11.47 -4.74 -19.74 -18.46 -9.41 -4.52 -6.88 -18.63 -23.87 -16.37 -10.29
Vacuole Periphery 0.06 3.15 -0.54 -0.99 -7.34 0.28 5.41 0.06 -0.23 -4.75 -1.45 6.80 -1.75 -0.06 -8.53
Standard name
Human Ortholog
Description Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
Localization
Cell Percentages mitochondrion (35%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Sam37

Sam37


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam37-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available