Standard name
Human Ortholog
Description Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; also required during meiosis; relocalizes to the cytosol in response to hypoxia
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0.09 0.09 0.1 0.09 0.12 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.36 0.49 0.27 0.46 0.37 0.32 0.49 0.54 0.48 0.47 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.79 0.8 0.79 0.76 0.65 0.68 0.65 0.62 0.59 0.6 0.64 0.62 0.69 0.71 0.59 0.67 0.72 0.81 0.75 0.76 0.71 0.86 0.72 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.2 0.19 0.21 0.26 0.32 0.27 0.27 0.3 0.3 0.26 0.28 0.3 0.27 0.25 0.35 0.21 0.13 0.14 0.18 0.14 0.16 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0.07 0.17 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 2 2 3 2 5 8 4 6 3 2 0 0 0 0 0 0 3 6 1 0 0 2
Bud Neck 0 0 0 0 1 0 1 3 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 1 4 14 20 20 29 15 37 27 28 0 0 1 0 0 1 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 13 7 11 13 13 21 18 14 10 17 6 12 11 14 37 25 14 0 0 1 1 1 0
Endoplasmic Reticulum 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 9 5 4 0 0 1 0 0 0
Endosome 5 2 2 0 3 0 0 0 0 0 1 0 0 0 1 1 6 0 4 5 7 0 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 1 0 0 2 0
Mitochondria 1 11 139 117 116 224 236 253 211 318 166 194 1 0 6 8 1 7 3 4 3 0 2 7
Nucleus 301 339 308 183 283 330 422 497 253 358 222 256 90 87 83 282 151 241 292 339 282 63 109 106
Nuclear Periphery 6 13 5 2 10 9 17 15 15 22 18 12 0 1 0 9 1 5 2 2 2 0 0 1
Nucleolus 77 81 82 62 139 133 171 243 127 151 98 121 36 31 49 90 28 43 70 63 65 0 3 8
Peroxisomes 2 1 0 0 0 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0
SpindlePole 5 0 3 2 7 5 6 1 2 0 1 1 1 3 1 1 1 1 5 3 9 0 0 2
Vac/Vac Membrane 4 10 2 1 2 1 3 3 1 8 0 3 1 0 1 25 13 8 7 13 17 5 25 57
Unique Cell Count 379 426 390 240 434 485 645 803 430 592 349 410 131 122 141 423 211 299 392 444 397 74 152 196
Labelled Cell Count 407 472 551 385 591 737 902 1072 642 912 553 625 141 133 157 463 232 326 392 444 397 74 152 196


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 6.9 4.6 4.2 4.8 4.5 4.5 4.6 4.2 4.1 4.1 4.1 7.8 7.2 6.5 11.0 11.5 11.3 6.4 7.0 7.1
Std Deviation (1e-4) 1.4 1.5 1.1 1.0 1.2 1.2 1.1 1.1 1.2 1.1 1.0 1.0 2.3 2.0 2.1 3.2 3.7 3.0 1.4 1.8 1.5
Intensity Change (Log2) – – – -0.12 0.06 -0.05 -0.03 0.0 -0.12 -0.15 -0.16 -0.18 0.75 0.65 0.5 1.26 1.31 1.29 0.48 0.61 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.0 1.1 0.9 1.4 0.5 1.3 -0.1 2.4 -0.4 0 0 4.2 4.4 5.2 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -7.8 -7.7 -7.2 -12.5 -9.7 -10.6
Nucleus -0.8 -4.4 -3.6 -4.6 -5.9 -6.2 -6.1 -4.6 -5.1 -2.4 -1.8 -4.6 -3.9 -2.0 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1.5 1.0 3.2 0.1 -2.3 -2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 4.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0 0 0 0 0 0.0001 – – – – – – 0.0093 0 0.0001 0 0 0
Bud 0 0 0 0 0 0 – – – – – – 0 0 0.0001 0 0 0
Bud Neck 0.0001 0.0001 0.0002 0.0001 0.0002 0.0007 – – – – – – 0.0005 0.0001 0.0001 0.0001 0.0002 0.0006
Bud Periphery 0 0 0.0001 0 0 0 – – – – – – 0 0 0.0001 0 0 0
Bud Site 0.0001 0.0001 0.0001 0.0001 0 0 – – – – – – 0.0001 0 0.0003 0.0001 0 0
Cell Periphery 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Cytoplasm 0.0001 0 0 0 0 0 – – – – – – 0.0002 0 0.0002 0 0 0
Cytoplasmic Foci 0 0 0 0 0 0 – – – – – – 0.0004 0 0.0005 0 0.0001 0
Eisosomes 0 0 0 0 0 0 – – – – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – – – – – – 0.0002 0 0.0001 0 0 0
Endosome 0 0 0 0 0 0 – – – – – – 0.0008 0 0.0048 0 0 0.0001
Golgi 0 0 0 0 0 0 – – – – – – 0.001 0 0.002 0 0 0
Lipid Particles 0 0 0 0 0 0 – – – – – – 0.0005 0 0.0002 0 0.0001 0
Mitochondria 0.0001 0.0001 0.0003 0 0.0002 0.0001 – – – – – – 0.0002 0 0.003 0.0001 0.0002 0.0001
None 0 0 0 0 0 0 – – – – – – 0.0001 0 0 0 0 0
Nuclear Periphery 0.0004 0.0004 0.0024 0.0003 0.0013 0.0014 – – – – – – 0.0004 0.001 0.001 0.0012 0.0006 0.003
Nucleolus 0.0541 0.054 0.1084 0.0817 0.0413 0.042 – – – – – – 0.0346 0.0678 0.0828 0.0912 0.0639 0.0814
Nucleus 0.9377 0.9343 0.8613 0.8844 0.9456 0.9514 – – – – – – 0.9497 0.8909 0.8966 0.9056 0.8797 0.9129
Peroxisomes 0 0 0 0 0 0 – – – – – – 0.0002 0 0.0001 0 0 0
Punctate Nuclear 0.0074 0.0107 0.0263 0.0332 0.0112 0.0041 – – – – – – 0.0014 0.04 0.0076 0.0016 0.055 0.0014
Vacuole 0 0.0001 0.0004 0 0.0001 0 – – – – – – 0.0002 0 0.0003 0 0.0001 0.0001
Vacuole Periphery 0 0.0001 0.0003 0 0 0 – – – – – – 0 0 0.0002 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.9268 18.009 5.5363 16.5348 46.6423 37.9679 15.4775 10.0074 10.6766 49.7116
Translational Efficiency 0.9306 0.8223 0.7066 0.5527 0.7503 1.5824 0.9699 0.6424 0.7791 0.8336

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; also required during meiosis; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pds5

Pds5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pds5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available