Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.17 0 0.09 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.09 0.07 0.05 0.12 0.11 0.1 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.85 0.82 0.88 0.84 0.83 0.7 0.69 0.63 0.65 0.62 0.59 0.6 0.85 0.79 0.75 0.85 0.74 0.72 0.76 0.8 0.72 0.75 0.73 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 0.41 0.29 0.38 0.31 0.52 0.47 0.48 0.55 0.6 0.61 0.62 0.24 0.15 0.18 0.19 0.24 0.21 0.18 0.14 0.24 0.19 0.21 0.33
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 1 8 0 1 5 4 6 0 0 0 1 0 0 0 1 1 0 1 0
Bud Neck 0 0 0 1 3 2 1 1 2 8 5 4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2 1 7 18 22 10 13 23 28 19 0 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 3 4 9 5 18 19 7 7 7 9 28 39 62 6 9 6 0 0 0 0 1 1
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 0 2 1 1 0 0 0 1 0 0 0 0 1 7 1 3 1 0 0 3 4 3 2 6
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 1 1 0 2
Mitochondria 5 8 9 3 10 65 80 48 27 107 83 80 0 0 0 7 3 0 0 0 2 0 0 5
Nucleus 99 249 627 367 357 573 621 436 377 570 452 473 316 288 275 127 70 41 89 255 534 205 303 212
Nuclear Periphery 0 0 5 1 2 4 8 3 5 9 7 4 1 1 0 3 0 1 0 0 0 0 0 0
Nucleolus 51 125 209 165 134 428 425 333 321 549 463 493 88 54 66 29 23 12 20 46 180 51 87 123
Peroxisomes 0 1 1 0 1 1 1 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 2 2 0 1 0 2 4 5 0 0 4 12 9 0 0 1 0 1 4 1 0 3
Vac/Vac Membrane 0 3 2 1 2 3 4 4 0 1 3 1 1 4 3 2 4 1 3 6 8 6 17 11
Unique Cell Count 117 303 715 435 432 823 902 688 581 913 762 794 372 366 369 150 95 57 117 320 742 274 415 375
Labelled Cell Count 156 393 864 546 525 1101 1188 858 757 1284 1052 1089 440 405 420 179 111 63 117 320 742 274 415 375


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 12.3 9.6 9.1 8.9 7.7 8.0 8.0 7.9 7.5 7.3 7.2 11.3 11.4 10.8 11.4 10.3 11.7 12.0 12.8 13.5
Std Deviation (1e-4) 1.8 4.0 2.1 2.4 2.5 2.2 2.5 2.1 2.2 2.2 2.4 2.5 3.0 3.2 3.4 3.6 2.8 3.6 2.7 2.8 2.9
Intensity Change (Log2) – – – -0.08 -0.12 -0.31 -0.26 -0.26 -0.28 -0.36 -0.39 -0.41 0.23 0.25 0.17 0.25 0.1 0.29 0.32 0.42 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.3 3.6 2.4 3.3 3.6 4.6 3.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.8 3.5 0 0 0 1.6 6.7 8.2 10.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.4 6.1 6.7 5.4 3.7 8.1 7.7 7.3 0 0 0 0 0 0
Nucleus -1.6 -2.4 -8.5 -9.0 -10.6 -9.8 -11.5 -12.3 -12.3 -1.3 -3.9 -5.5 -1.0 -3.7 -3.4
Nuclear Periphery 0 0 0 0.4 0 0 0.6 0.5 0 0 0 0 0 0 0
Nucleolus 3.1 0.6 9.0 7.3 7.4 9.5 12.4 12.2 12.8 -2.0 -5.2 -4.1 -2.5 -1.0 -1.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8377 9.4954 8.7552 8.0454 7.7485 8.7844 8.5361 8.0504 7.2441 7.0005 6.9986 7.6468 9.6653 8.0936 7.9385 8.0217 6.8438 7.5129
Actin 0.0272 0 0.0005 0 0.0012 0.0042 0.0352 0 0.0002 0.0037 0.004 0 0.0069 0 0 0 0 0
Bud 0.0002 0 0.0002 0 0.0001 0 0.0008 0 0.0002 0.0001 0.0003 0 0.0001 0 0 0 0 0
Bud Neck 0.0003 0 0.0001 0 0.0003 0.0003 0.001 0 0.0004 0.0001 0.0004 0.0003 0.0003 0 0 0 0.0002 0.0002
Bud Periphery 0.0003 0 0.0004 0 0.0001 0.0001 0.0017 0 0.0004 0.0001 0.0005 0.0001 0.0001 0 0 0 0.0001 0
Bud Site 0.0003 0 0.0002 0 0.0002 0.0001 0.0017 0 0.0036 0.0005 0.0005 0 0.0013 0 0 0 0 0.0001
Cell Periphery 0.0001 0 0.0002 0 0 0 0.0004 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0 0
Cytoplasm 0.0103 0 0.0006 0 0.0007 0.0001 0.0149 0 0.0002 0.0132 0.0005 0 0.0021 0 0 0 0 0
Cytoplasmic Foci 0.0015 0 0.0054 0 0.0406 0.0009 0.0141 0 0.0044 0.0096 0.0166 0 0.0079 0 0 0 0 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0022 0 0.0005 0 0.0001 0.0001 0.0074 0 0.0001 0.0011 0.0005 0 0.0006 0 0 0 0 0
Endosome 0.0156 0 0.0017 0 0.0056 0.0011 0.0263 0 0.0013 0.0149 0.0189 0 0.0038 0 0 0 0 0.0001
Golgi 0.0017 0 0.0005 0 0.0027 0.001 0.005 0 0.0005 0.0043 0.0135 0 0.0013 0 0 0 0 0
Lipid Particles 0.0018 0 0.0093 0 0.0382 0.0013 0.0102 0 0.0045 0.0156 0.0284 0 0.0056 0 0 0 0 0.0003
Mitochondria 0.0021 0.0001 0.0104 0 0.0009 0.0003 0.0164 0.0001 0.0105 0.0061 0.0201 0.0001 0.0011 0 0 0.0001 0.0007 0.0008
None 0.003 0 0.0005 0 0.0004 0.0001 0.0154 0 0.0002 0.002 0.001 0 0.0035 0 0 0 0 0.0001
Nuclear Periphery 0.0194 0.0013 0.0045 0.0001 0.0008 0.0001 0.0285 0.0005 0.0042 0.0027 0.0053 0.0002 0.005 0.0001 0.0001 0.0001 0.0003 0.0002
Nucleolus 0.0687 0.025 0.0427 0.0215 0.2468 0.1658 0.076 0.055 0.0568 0.0218 0.2731 0.1924 0.0389 0.0313 0.0309 0.0419 0.288 0.1499
Nucleus 0.8339 0.9733 0.9085 0.9783 0.633 0.8223 0.704 0.9438 0.9067 0.8933 0.5649 0.806 0.91 0.9667 0.9689 0.9579 0.7098 0.8475
Peroxisomes 0.0021 0 0.0006 0 0.0082 0.0015 0.0049 0 0.0025 0.0054 0.0209 0 0.0065 0 0 0 0 0
Punctate Nuclear 0.0063 0.0001 0.0104 0.0001 0.0197 0.0002 0.0301 0.0004 0.0015 0.0043 0.0278 0.0002 0.0045 0.0018 0 0 0.0007 0.0003
Vacuole 0.0012 0 0.0011 0 0.0002 0.0003 0.0031 0 0.0008 0.0004 0.0008 0.0002 0.0002 0 0 0 0.0001 0.0001
Vacuole Periphery 0.0017 0 0.0018 0 0.0002 0.0001 0.0029 0 0.0007 0.0005 0.0017 0.0001 0.0002 0 0 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.1791 44.4925 44.207 45.2077 47.0308 30.3521 39.8396 36.2809 42.8463 44.0417
Translational Efficiency 2.3175 2.189 1.8565 2.1861 1.9672 2.9339 1.8005 2.2703 1.7761 1.8602

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1801 1017 1262 1052 1922 144 2136 1441 3723 1161 3398 2493

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1004.62 1127.95 1193.91 1334.35 1037.06 1196.69 1353.94 1320.37 1021.37 1136.48 1294.51 1326.27
Standard Deviation 180.53 232.73 251.91 271.95 187.82 206.96 231.35 296.32 185.04 230.81 251.38 286.37
Intensity Change Log 2 — 0.167053 0.249044 0.409487 — 0.206550 0.384664 0.348443 — 0.187251 0.319524 0.378803

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000462 0.000672 0.000993 0.001084 0.000428 0.000719 0.000629 0.001196 0.000445 0.000678 0.000764 0.001149
Bud Neck 0.004047 0.004890 0.010588 0.015822 0.004343 0.005516 0.010689 0.018403 0.004200 0.004968 0.010651 0.017314
Bud Site 0.000443 0.000765 0.001210 0.003529 0.000586 0.000555 0.000868 0.003445 0.000517 0.000739 0.000995 0.003480
Cell Periphery 0.000025 0.000040 0.000062 0.000104 0.000035 0.000017 0.000042 0.000137 0.000030 0.000037 0.000049 0.000124
Cytoplasm 0.000279 0.000225 0.000291 0.000756 0.000042 0.000080 0.000059 0.001695 0.000157 0.000207 0.000145 0.001299
Cytoplasmic Foci 0.000538 0.000120 0.000120 0.001970 0.000025 0.000552 0.000088 0.002088 0.000273 0.000173 0.000100 0.002038
Eisosomes 0.000102 0.000099 0.000110 0.000080 0.000118 0.000072 0.000122 0.000058 0.000110 0.000096 0.000118 0.000067
Endoplasmic Reticulum 0.000897 0.000789 0.000965 0.000743 0.000900 0.001086 0.000495 0.000982 0.000898 0.000825 0.000670 0.000881
Endosome 0.000044 0.000124 0.000053 0.002566 0.000037 0.000226 0.000070 0.002505 0.000040 0.000136 0.000064 0.002531
Golgi 0.000074 0.000122 0.000125 0.001777 0.000040 0.000311 0.000062 0.002316 0.000057 0.000146 0.000085 0.002089
Lipid Particles 0.000042 0.000153 0.000018 0.000159 0.000011 0.000102 0.000018 0.000245 0.000026 0.000146 0.000018 0.000209
Mitochondria 0.000538 0.002076 0.001847 0.005164 0.000682 0.001297 0.001392 0.004214 0.000612 0.001980 0.001561 0.004615
Mitotic Spindle 0.001424 0.004114 0.002892 0.012452 0.000983 0.037027 0.000869 0.011404 0.001197 0.008197 0.001620 0.011846
None 0.000635 0.000967 0.002195 0.001570 0.000510 0.000680 0.000522 0.002753 0.000570 0.000931 0.001143 0.002254
Nuclear Periphery 0.000044 0.000072 0.000120 0.000441 0.000026 0.000136 0.000218 0.001026 0.000035 0.000080 0.000182 0.000779
Nuclear Periphery Foci 0.000199 0.000256 0.000083 0.000345 0.000030 0.001054 0.000086 0.001367 0.000112 0.000355 0.000085 0.000936
Nucleolus 0.135436 0.145512 0.114528 0.099242 0.112678 0.159315 0.110128 0.095935 0.123687 0.147224 0.111762 0.097330
Nucleus 0.852661* 0.834057* 0.857683* 0.843139* 0.876459* 0.785458* 0.870435* 0.837834* 0.864946* 0.828029* 0.865699* 0.840073*
Peroxisomes 0.001826 0.004195 0.005686 0.004375 0.001676 0.005166 0.002704 0.003367 0.001749 0.004315 0.003811 0.003792
Vacuole 0.000123 0.000391 0.000228 0.003022 0.000164 0.000380 0.000215 0.005270 0.000144 0.000390 0.000220 0.004321
Vacuole Periphery 0.000163 0.000361 0.000203 0.001661 0.000227 0.000253 0.000289 0.003761 0.000196 0.000347 0.000257 0.002874

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.54 -8.21 -8.62 -6.38 -2.63 -4.49 -10.17 -8.26 -4.74 -6.16 -4.05 -9.91 -11.16 -6.90 -6.98
Bud Neck -1.73 -18.51 -11.65 -10.07 -5.34 -1.15 -14.32 -15.48 -10.49 -8.63 -1.71 -21.50 -19.43 -16.14 -10.03
Bud Site -2.05 -8.43 -4.35 -3.89 -3.25 0.18 -1.59 -6.41 -6.73 -5.99 -1.46 -4.44 -7.61 -6.99 -6.46
Cell Periphery -1.68 -4.65 -4.04 -3.19 -2.32 1.49 -0.52 -4.84 -7.12 -4.93 -0.73 -2.16 -6.47 -5.97 -5.27
Cytoplasm 0.76 0.67 -1.07 -2.55 -2.48 -0.96 -1.15 -3.16 -3.09 -3.12 0.39 0.73 -2.98 -3.64 -3.93
Cytoplasmic Foci 1.00 1.00 -1.44 -2.66 -2.65 -1.18 -2.02 -3.77 -2.25 -3.65 0.55 0.88 -3.48 -4.30 -4.52
Eisosomes 0.37 -1.89 4.33 3.19 6.94 5.97 -0.77 10.43 1.88 15.04 2.52 -2.08 11.11 5.45 16.03
Endoplasmic Reticulum 1.10 -1.16 0.55 -0.43 1.43 -1.06 9.60 -2.61 -0.36 -6.61 0.82 5.72 -1.82 -2.04 -5.01
Endosome -1.86 -0.53 -3.21 -3.08 -3.20 -1.07 -1.35 -4.36 -3.72 -4.29 -2.26 -1.38 -5.34 -5.06 -5.28
Golgi -1.39 -3.03 -2.47 -2.39 -2.38 -1.68 -4.91 -3.32 -2.73 -3.27 -2.69 -3.51 -4.13 -3.90 -4.05
Lipid Particles -1.04 1.11 -2.05 -0.01 -2.81 -2.37 -0.93 -3.10 -1.63 -2.99 -1.31 0.79 -3.63 -0.39 -3.99
Mitochondria -2.08 -19.84 -4.58 -2.37 -3.18 -2.49 -8.43 -9.80 -6.73 -7.57 -2.11 -16.64 -8.50 -3.15 -6.29
Mitotic Spindle -1.60 -1.47 -4.15 -2.74 -3.47 -2.45 -0.07 -5.22 1.72 -5.29 -3.02 -0.95 -6.59 -1.30 -6.24
None -1.34 -3.75 -2.42 -1.76 0.97 -1.23 -0.34 -4.56 -4.17 -4.56 -2.25 -3.32 -5.07 -3.95 -3.32
Nuclear Periphery -1.32 -4.47 -4.76 -4.43 -3.95 -1.64 -1.95 -4.56 -3.86 -3.30 -2.57 -2.36 -5.79 -5.39 -4.17
Nuclear Periphery Foci -0.31 1.71 -1.02 -0.59 -2.75 -1.05 -1.14 -2.60 -0.24 -2.48 -1.54 0.89 -2.69 -1.64 -2.86
Nucleolus -1.68 2.90 5.91 6.48 2.95 -3.89 0.20 2.29 4.71 1.99 -4.59 2.56 5.93 8.33 3.36
Nucleus 2.76 0.15 1.68 -0.63 1.47 5.01 1.80 6.92 -2.40 5.41 6.23 0.82 6.05 -0.93 5.20
Peroxisomes -5.45 -8.26 -6.00 -0.19 3.18 -4.06 -6.03 -3.40 1.66 -1.31 -7.29 -9.60 -5.76 1.00 0.37
Vacuole -1.92 -4.37 -4.25 -3.81 -4.12 -1.24 -1.43 -4.90 -4.62 -4.84 -2.02 -3.00 -6.33 -5.84 -6.21
Vacuole Periphery -4.22 -4.75 -3.36 -2.84 -3.25 -0.50 -1.66 -4.15 -4.10 -4.06 -3.57 -2.80 -5.12 -4.75 -4.98
Standard name
Human Ortholog
Description Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Npl6

Npl6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npl6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available