Standard name | |
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Human Ortholog | |
Description | Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 1 | 1 | 0 | – | 0 | 1 | 2 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 9 | 4 | 0 | 8 | 28 | 49 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 0 | 0 | 0 | 3 | – | 1 | 1 | 1 | 9 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 172 | 157 | – | 29 | 192 | 68 | 158 | – | 96 | 86 | 139 | 179 | 254 | 438 | 544 | 142 | 78 | 18 | 153 | 148 | 18 | 177 | 281 | 377 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 3 | 0 | 4 | – | 2 | 0 | 2 | 6 | 2 | 3 | 0 | 9 | 3 | 1 | 1 | 0 | 0 | 1 | 5 | 4 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 2 |
Golgi | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | – | 0 | 0 | 4 | 3 | – | 16 | 13 | 35 | 34 | 0 | 0 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleus | 0 | 0 | – | 0 | 1 | 1 | 6 | – | 4 | 3 | 5 | 11 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 3 | 2 | 5 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | – | 0 | 1 | 0 | 1 | – | 0 | 2 | 1 | 6 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 8 |
Vac/Vac Membrane | 4 | 2 | – | 3 | 15 | 19 | 32 | – | 26 | 20 | 18 | 42 | 7 | 7 | 6 | 5 | 0 | 0 | 0 | 4 | 1 | 1 | 5 | 4 |
Unique Cell Count | 178 | 159 | 31 | 208 | 90 | 196 | 132 | 124 | 186 | 259 | 265 | 444 | 551 | 146 | 82 | 18 | 176 | 167 | 24 | 203 | 339 | 462 | ||
Labelled Cell Count | 179 | 160 | 32 | 214 | 93 | 208 | 145 | 131 | 205 | 296 | 268 | 450 | 557 | 162 | 85 | 19 | 176 | 167 | 24 | 203 | 339 | 462 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 23.7 | 25.1 | – | 26.8 | 19.8 | 9.8 | 12.3 | – | 7.6 | 8.0 | 6.9 | 6.8 | 30.7 | 26.5 | 28.7 | 35.4 | 34.1 | 45.5 | 22.5 | 24.7 | 23.7 |
Std Deviation (1e-4) | 14.2 | 13.6 | – | 9.6 | 7.7 | 4.3 | 5.2 | – | 3.0 | 2.9 | 2.6 | 2.6 | 13.5 | 12.8 | 13.0 | 16.6 | 16.5 | 13.2 | 10.3 | 11.9 | 12.3 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.0764 | 29.2501 | 28.3117 | 22.4256 | 21.0266 | 25.3653 | 30.8562 | 41.7429 | 38.5243 | 31.9172 | 30.7314 | 35.2823 | 25.9397 | 32.6393 | 32.6236 | 27.7794 | 28.1848 | 30.5443 |
Actin | 0.0024 | 0.0009 | 0.0039 | 0.0008 | 0.0037 | 0.001 | 0.0031 | 0.0007 | 0.0008 | 0.026 | 0.0118 | 0.0017 | 0.0056 | 0.0003 | 0.001 | 0.0004 | 0.0008 | 0.0013 |
Bud | 0.0009 | 0.0011 | 0.0057 | 0.0018 | 0.0012 | 0.0038 | 0.0003 | 0.0016 | 0.0044 | 0.0025 | 0.0015 | 0.0014 | 0.0023 | 0.0035 | 0.0035 | 0.0003 | 0.0008 | 0.0027 |
Bud Neck | 0.0002 | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0008 | 0.0003 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0008 | 0.0007 |
Bud Periphery | 0.001 | 0.0002 | 0.0021 | 0.0008 | 0.001 | 0.0019 | 0.0001 | 0.0005 | 0.0011 | 0.0022 | 0.0008 | 0.0006 | 0.0014 | 0.0017 | 0.001 | 0.0001 | 0.0005 | 0.0036 |
Bud Site | 0.0004 | 0.0018 | 0.0021 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0021 | 0.0006 | 0.0013 | 0.0002 | 0.0002 | 0.001 | 0.0044 | 0.0013 | 0.0001 | 0.0001 | 0.0003 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.8351 | 0.9792 | 0.9155 | 0.9187 | 0.8302 | 0.9138 | 0.9064 | 0.9714 | 0.9618 | 0.9341 | 0.8146 | 0.9353 | 0.8766 | 0.9721 | 0.9541 | 0.927 | 0.9023 | 0.9005 |
Cytoplasmic Foci | 0.0161 | 0.0005 | 0.0072 | 0.0012 | 0.0176 | 0.004 | 0.0086 | 0.0042 | 0.0015 | 0.0018 | 0.0318 | 0.0012 | 0.0077 | 0.0008 | 0.0029 | 0.0017 | 0.0027 | 0.0036 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0034 | 0.0004 | 0.0008 | 0.0012 | 0.0061 | 0.0014 | 0.0026 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0021 | 0.0005 | 0.0015 | 0.0008 | 0.0021 | 0.0023 |
Endosome | 0.0055 | 0.0001 | 0.0031 | 0.0007 | 0.0147 | 0.0034 | 0.007 | 0.0006 | 0.0018 | 0.0004 | 0.0019 | 0.0002 | 0.0034 | 0.0002 | 0.0024 | 0.0005 | 0.0013 | 0.0027 |
Golgi | 0.0013 | 0 | 0.0012 | 0 | 0.0009 | 0.0005 | 0.0014 | 0.0003 | 0.0006 | 0.0005 | 0.0026 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0.0007 |
Lipid Particles | 0.0045 | 0 | 0.0039 | 0 | 0.0069 | 0.0026 | 0.0067 | 0.0005 | 0.0013 | 0.0002 | 0.025 | 0 | 0.0016 | 0 | 0.0005 | 0 | 0.0003 | 0.0006 |
Mitochondria | 0.0094 | 0.0001 | 0.0039 | 0.0001 | 0.0098 | 0.0003 | 0.0029 | 0.0004 | 0.0001 | 0.0002 | 0.0477 | 0.0002 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0015 | 0.0005 |
None | 0.0214 | 0.0022 | 0.007 | 0.0023 | 0.0028 | 0.0116 | 0.0063 | 0.0101 | 0.0034 | 0.0052 | 0.0007 | 0.0222 | 0.0244 | 0.0055 | 0.0081 | 0.0328 | 0.0459 | 0.0144 |
Nuclear Periphery | 0.0126 | 0.0022 | 0.0028 | 0.0096 | 0.017 | 0.0062 | 0.008 | 0.0005 | 0.0024 | 0.0016 | 0.0027 | 0.0031 | 0.0086 | 0.0011 | 0.0035 | 0.0027 | 0.007 | 0.0058 |
Nucleolus | 0.0003 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0011 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0003 |
Nucleus | 0.0781 | 0.0104 | 0.0285 | 0.0586 | 0.0278 | 0.046 | 0.0369 | 0.0043 | 0.0177 | 0.02 | 0.0204 | 0.0314 | 0.0541 | 0.008 | 0.0156 | 0.0318 | 0.0277 | 0.0515 |
Peroxisomes | 0.0007 | 0 | 0.0098 | 0 | 0.0371 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0007 | 0.0306 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0014 | 0.0004 | 0.0008 | 0.0017 | 0.0043 | 0.0009 | 0.0019 | 0.0002 | 0.0011 | 0.0007 | 0.0046 | 0.0006 | 0.0051 | 0.0006 | 0.0013 | 0.0009 | 0.001 | 0.0069 |
Vacuole | 0.0025 | 0.0002 | 0.0007 | 0.0014 | 0.0127 | 0.0008 | 0.0028 | 0.001 | 0.0002 | 0.0011 | 0.0016 | 0.0005 | 0.0022 | 0.0006 | 0.0015 | 0.0003 | 0.0028 | 0.0009 |
Vacuole Periphery | 0.0026 | 0 | 0.0003 | 0.0004 | 0.0042 | 0.0003 | 0.0026 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0016 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.0681 | 10.2291 | 3.253 | 2.7904 | 1.0597 | 24.5065 | 24.7484 | 17.95 | 23.6847 | 19.4673 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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