Standard name
Human Ortholog
Description Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; not an essential gene; YMR102C has a paralog, DGR2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.23 0.2 0.15 0.18 0.23 0.15
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0.06 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0.08 0 0 0 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0.08 0.07 0.05 0.09 0.05 0 0 0 0 0 0 0 0.08 0.15 0.11 0 – – 0.11 0.13 0.17 0.17 0.19 0.12
Cytoplasm 0.92 0.95 0.96 0.97 0.93 0.94 0.88 0.89 0.84 0.8 0.71 0.76 0.96 0.97 0.97 1.0 – – 0.5 0.46 0.41 0.48 0.39 0.49
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.13 0.07 0.29 0.3 0.38 0.3 0 0 0 0 – – 0.05 0.07 0.14 0.07 0.08 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 6 6 1 5 1 6 3 1 1 0 1 5 3 2 0 – – 34 57 52 40 61 41
Bud 3 7 6 2 4 3 10 11 10 14 11 20 15 1 6 0 – – 0 0 3 1 1 7
Bud Neck 0 0 3 2 1 1 5 8 2 3 0 1 0 0 0 0 – – 3 22 14 8 10 19
Bud Site 0 0 0 0 0 1 0 0 0 2 2 0 0 0 0 0 – – – – – – – –
Cell Periphery 10 17 19 18 14 9 9 7 8 6 1 7 21 47 40 0 – – 16 38 58 38 52 31
Cytoplasm 118 245 337 194 257 247 333 288 254 226 156 199 258 305 339 20 – – 73 135 140 111 105 132
Endoplasmic Reticulum 2 2 2 0 4 1 1 1 0 0 1 0 1 5 2 0 – – 4 5 7 2 5 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 – – 2 5 3 1 3 3
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 – – 0 1 0 0 0 2
Mitochondria 3 0 2 3 11 23 49 23 88 84 83 78 1 0 0 0 – – 7 20 48 16 21 11
Nucleus 0 0 2 4 3 3 4 9 4 6 7 0 0 0 0 0 – – 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 – – 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 1 0 0 0 0 1 0 – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 2 0 2 1 2 6 0 2 5 4 1 1 0 0 – – 0 0 1 0 0 0
Vac/Vac Membrane 0 0 0 1 1 1 2 0 1 1 0 5 1 0 0 0 – – 1 2 2 4 5 5
Unique Cell Count 128 257 350 199 276 264 379 325 301 282 221 262 270 315 348 20 147 296 343 231 272 269
Labelled Cell Count 140 277 380 225 303 291 422 356 369 347 266 315 304 362 392 20 147 296 343 231 272 269


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.8 5.5 5.5 5.7 4.5 4.5 4.7 3.9 3.9 3.8 4.1 5.3 5.8 5.8 7.6 – – 4.8 4.7 5.5
Std Deviation (1e-4) 0.6 0.6 1.2 2.0 1.7 1.7 1.3 1.5 1.4 1.4 1.4 1.1 1.0 1.2 1.2 1.1 – – 1.4 0.9 1.9
Intensity Change (Log2) – – – -0.01 0.05 -0.28 -0.28 -0.22 -0.49 -0.49 -0.54 -0.42 -0.07 0.06 0.08 0.47 – – -0.19 -0.22 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0814 3.9588 3.584 3.152 2.872 3.4261 1.7137 2.1268 1.6923 0.8579 3.0603 1.6157 1.529 1.8441 1.6058 1.483 1.3039 1.6264
Actin 0.0841 0.0695 0.0607 0.0981 0.0761 0.0568 0.1238 0.0677 0.1196 0.0895 0.1101 0.1298 0.0833 0.0528 0.0736 0.1303 0.073 0.0573
Bud 0.0016 0.0009 0.0006 0.0018 0.0005 0.0005 0.0058 0.0032 0.0044 0.0008 0.0026 0.0026 0.0029 0.0009 0.0027 0.0036 0.0055 0.0005
Bud Neck 0.0245 0.0089 0.018 0.0444 0.0861 0.0706 0.013 0.0081 0.0388 0.1355 0.0195 0.0449 0.022 0.0072 0.0264 0.0838 0.0301 0.0457
Bud Periphery 0.0031 0.0019 0.0017 0.0065 0.0014 0.0025 0.0118 0.0054 0.0091 0.0024 0.0055 0.0074 0.0086 0.0019 0.0075 0.0115 0.0112 0.0021
Bud Site 0.0372 0.1023 0.0278 0.0098 0.0037 0.0025 0.0396 0.1814 0.0566 0.0234 0.0066 0.0035 0.0128 0.0967 0.0273 0.0202 0.0102 0.0011
Cell Periphery 0.0195 0.0332 0.0234 0.0199 0.0292 0.0216 0.0172 0.0307 0.0246 0.0169 0.0181 0.0135 0.0158 0.0164 0.0151 0.0176 0.0064 0.0072
Cytoplasm 0.4131 0.6195 0.6263 0.6182 0.7465 0.7301 0.5392 0.6086 0.6057 0.6118 0.3836 0.6754 0.5333 0.6728 0.6768 0.5755 0.5844 0.683
Cytoplasmic Foci 0.0682 0.0065 0.0167 0.026 0.0079 0.0092 0.0295 0.0077 0.0174 0.0186 0.0486 0.0149 0.0244 0.0113 0.0152 0.0115 0.0741 0.0159
Eisosomes 0.0024 0.0021 0.0014 0.0019 0.0007 0.0013 0.003 0.0024 0.0026 0.0007 0.0017 0.002 0.0026 0.0009 0.0013 0.001 0.0004 0.0006
Endoplasmic Reticulum 0.0075 0.0043 0.0058 0.0054 0.0037 0.0033 0.019 0.0048 0.0064 0.0097 0.0088 0.0043 0.0107 0.0052 0.0048 0.0069 0.0095 0.0042
Endosome 0.0352 0.0003 0.0059 0.0189 0.0003 0.0004 0.0316 0.0012 0.0078 0.0041 0.1069 0.0056 0.0253 0.0009 0.0032 0.0028 0.011 0.0045
Golgi 0.018 0.0014 0.0071 0.0253 0.0011 0.0013 0.0186 0.0024 0.008 0.0076 0.0557 0.007 0.0076 0.002 0.0038 0.003 0.0098 0.0038
Lipid Particles 0.0405 0.0009 0.0048 0.0147 0.0005 0.0005 0.0126 0.0011 0.0041 0.0055 0.0268 0.0059 0.0096 0.0011 0.0032 0.0026 0.0094 0.0038
Mitochondria 0.0215 0.0001 0.0087 0.0441 0.0001 0.0002 0.017 0.0002 0.0107 0.0048 0.0378 0.0023 0.0036 0.0002 0.0012 0.0129 0.0048 0.0008
None 0.1528 0.146 0.1832 0.0433 0.0408 0.0973 0.0764 0.07 0.0725 0.0571 0.035 0.0725 0.1967 0.126 0.1247 0.1013 0.1025 0.1621
Nuclear Periphery 0.0126 0.0003 0.0004 0.0021 0.0001 0.0001 0.0099 0.0005 0.0011 0.001 0.037 0.001 0.0089 0.0003 0.002 0.0005 0.0074 0.0005
Nucleolus 0.0048 0.0001 0.0002 0.0007 0.0001 0.0001 0.0008 0.0005 0.0006 0.0002 0.0024 0.0005 0.0017 0.0002 0.0006 0.0002 0.0007 0.0001
Nucleus 0.0058 0.0004 0.0006 0.0014 0.0004 0.0004 0.0115 0.001 0.0016 0.0012 0.0302 0.0016 0.0043 0.0006 0.002 0.001 0.0142 0.0005
Peroxisomes 0.0302 0.0005 0.0037 0.0097 0.0004 0.0006 0.0089 0.0007 0.0034 0.0058 0.0337 0.0025 0.0072 0.0009 0.0048 0.0122 0.0193 0.0044
Punctate Nuclear 0.0062 0.0003 0.0005 0.0014 0.0002 0.0002 0.0045 0.0007 0.0025 0.0025 0.0108 0.0017 0.0133 0.0008 0.0029 0.0008 0.0138 0.0015
Vacuole 0.0085 0.0005 0.0021 0.0043 0.0003 0.0004 0.0049 0.0017 0.0023 0.0008 0.0119 0.0008 0.0039 0.0008 0.0009 0.0008 0.0019 0.0004
Vacuole Periphery 0.0029 0 0.0004 0.0022 0 0 0.0013 0.0001 0.0005 0.0002 0.0068 0.0003 0.0012 0 0.0001 0.0001 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3335 16.538 19.5767 25.1488 16.8475 20.6911 24.6843 28.618 26.5804 27.7037
Translational Efficiency 0.8796 0.7976 0.732 0.5885 0.6608 0.821 0.6248 0.6268 0.7173 0.5588

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1937 1821 1220 1345 1693 1740 345 122 3630 3561 1565 1467

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.05 830.85 943.36 955.88 678.41 742.16 810.41 1012.23 687.29 787.51 914.05 960.57
Standard Deviation 72.78 105.95 115.25 122.26 80.84 94.24 128.43 138.53 77.09 109.75 130.50 124.67
Intensity Change Log 2 — 0.257471 0.440692 0.459713 — 0.129573 0.256495 0.577308 — 0.195713 0.352645 0.518996

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005249 0.014742 0.008377 0.198947* 0.003408 0.015467 0.005899 0.252011* 0.004391 0.015096 0.007831 0.203360*
Bud Neck 0.046355 0.041115 0.016666 0.027162 0.024513 0.051081 0.008319 0.023214 0.036168 0.045984 0.014826 0.026834
Bud Site 0.013826 0.037278 0.036098 0.161466* 0.011736 0.034392 0.008909 0.231924* 0.012851 0.035868 0.030105 0.167325*
Cell Periphery 0.003288 0.002823 0.003283 0.033751 0.001189 0.002461 0.002517 0.057050 0.002309 0.002646 0.003114 0.035689
Cytoplasm 0.534461* 0.475242* 0.311292* 0.238491* 0.462374* 0.452336* 0.386948* 0.117927* 0.500840* 0.464050* 0.327970* 0.228464*
Cytoplasmic Foci 0.157258 0.187943* 0.036688 0.024005 0.234048* 0.215428* 0.192744* 0.027010 0.193072* 0.201373* 0.071090 0.024255
Eisosomes 0.014718 0.007864 0.000867 0.025399 0.003166 0.009180 0.001569 0.038082 0.009330 0.008507 0.001022 0.026453
Endoplasmic Reticulum 0.001531 0.003024 0.022362 0.017210 0.002136 0.001567 0.002839 0.029937 0.001813 0.002312 0.018058 0.018268
Endosome 0.003861 0.006539 0.004920 0.002526 0.008290 0.009517 0.011595 0.007434 0.005927 0.007994 0.006391 0.002934
Golgi 0.009065 0.010477 0.000347 0.001437 0.006277 0.014424 0.003840 0.000843 0.007765 0.012406 0.001117 0.001388
Lipid Particles 0.011253 0.007259 0.006645 0.004155 0.015568 0.012148 0.051482 0.008417 0.013265 0.009648 0.016529 0.004509
Mitochondria 0.004817 0.003281 0.000562 0.000513 0.002521 0.009690 0.000967 0.000160 0.003746 0.006412 0.000651 0.000484
Mitotic Spindle 0.000598 0.002229 0.001986 0.002676 0.000188 0.002595 0.000714 0.000513 0.000407 0.002408 0.001705 0.002496
None 0.002987 0.001054 0.001701 0.001115 0.003158 0.001765 0.013844 0.003599 0.003067 0.001401 0.004378 0.001321
Nuclear Periphery 0.000114 0.000148 0.001772 0.000162 0.000169 0.000200 0.000985 0.000128 0.000139 0.000173 0.001599 0.000159
Nuclear Periphery Foci 0.000292 0.000626 0.005261 0.001795 0.000503 0.000476 0.004047 0.002195 0.000390 0.000553 0.004993 0.001828
Nucleolus 0.000386 0.000297 0.000283 0.000047 0.000508 0.000460 0.002076 0.000042 0.000443 0.000377 0.000678 0.000047
Nucleus 0.147115* 0.120332 0.387859* 0.093117 0.165326* 0.089190 0.221709* 0.043457 0.155608* 0.105115 0.351232* 0.088987
Peroxisomes 0.012098 0.019469 0.001910 0.005028 0.014339 0.031138 0.010708 0.003239 0.013143 0.025171 0.003849 0.004879
Vacuole 0.030431 0.057427 0.150981 0.160512 0.040182 0.045603 0.068046 0.152782 0.034979 0.051649 0.132698 0.159869
Vacuole Periphery 0.000299 0.000832 0.000140 0.000489 0.000402 0.000882 0.000243 0.000036 0.000347 0.000857 0.000163 0.000451

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.86 -1.80 -23.93 -22.28* -23.42* -8.25 -1.39 -8.99 -8.48 -8.86 -10.63 -2.74 -25.63 -23.85 -25.03*
Bud Neck 1.79 11.88 8.18 6.33 -4.28 -9.23 7.34 0.71 6.97 -3.53 -4.57 12.50 5.92 10.29 -5.83
Bud Site -11.20 -7.88 -24.42 -19.35 -19.14 -10.73 0.59 -9.58 -8.48 -9.60 -15.47 -7.71 -26.40 -21.59 -22.03
Cell Periphery 1.73 1.10 -15.92 -16.19 -16.02 -4.26 -2.27 -5.57 -5.42 -5.40 -1.60 -1.96 -17.23 -16.98 -16.76
Cytoplasm 8.23 32.25 37.95 27.98 3.98 1.13 6.15 21.64 20.95 12.37 6.70 28.93 38.82 32.55 7.89
Cytoplasmic Foci -5.69 31.31 35.66 36.97 4.12 2.94 3.56 30.50 28.67 14.98 -1.81 28.36 51.08 52.13 13.06
Eisosomes 6.78 16.45 -4.16 -8.88 -14.34 -7.35 3.95 -4.79 -3.81 -5.05 1.28 17.09 -8.82 -9.40 -15.11
Endoplasmic Reticulum -3.32 -18.17 -13.99 -12.20 4.38 1.97 -2.67 -5.59 -5.70 -5.34 -1.88 -17.82 -14.92 -14.34 1.10
Endosome -6.18 -4.69 2.71 6.62 5.38 -1.40 -1.69 0.36 0.58 0.95 -4.45 -1.50 4.57 7.40 5.16
Golgi -1.38 15.05 9.42 9.41 -1.50 -7.81 2.13 9.30 13.15 2.22 -6.45 14.77 9.95 13.61 -0.23
Lipid Particles 5.59 5.90 10.65 5.80 6.39 2.23 -6.20 2.09 1.17 6.53 4.37 -2.37 11.95 7.41 8.73
Mitochondria 1.73 5.25 5.35 6.74 1.12 -5.90 5.47 9.32 8.02 5.13 -3.47 6.89 7.27 9.54 2.35
Mitotic Spindle -1.74 -1.98 -2.04 -0.48 -0.73 -3.89 -2.13 -1.08 3.01 0.52 -3.57 -2.60 -2.33 -0.21 -0.90
None 4.98 3.46 3.44 0.11 1.10 2.31 -3.24 -0.13 -0.55 2.11 4.71 -1.36 3.20 0.35 3.37
Nuclear Periphery -0.66 -26.12 -6.79 -1.07 24.71 -0.28 -8.08 0.14 0.31 7.90 -0.58 -26.58 -4.63 -0.48 25.42
Nuclear Periphery Foci -6.09 -17.62 -14.22 -10.85 11.53 0.18 -3.53 -4.66 -4.55 1.54 -2.37 -14.88 -14.31 -11.33 9.64
Nucleolus 1.87 1.59 12.55 6.19 7.22 -0.37 -1.64 7.77 3.00 2.09 0.08 -1.25 12.91 4.28 2.90
Nucleus 5.87 -27.92 10.37 4.72 33.17 15.31 -4.40 16.25 6.21 13.05 15.00 -26.20 14.72 2.97 33.30
Peroxisomes -5.10 13.61 7.90 10.59 -3.89 -8.89 1.64 9.67 15.97 4.11 -10.17 12.47 10.47 17.51 -0.76
Vacuole -12.75 -27.18 -26.86 -21.69 -0.96 -2.38 -5.11 -7.41 -7.16 -5.13 -10.92 -25.97 -27.25 -24.12 -4.14
Vacuole Periphery -1.69 6.96 -0.68 1.14 -1.56 -3.22 2.25 10.73 5.81 2.94 -2.88 7.28 -0.24 1.98 -1.38
Standard name
Human Ortholog
Description Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; not an essential gene; YMR102C has a paralog, DGR2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (26%), ER (3%), mixed (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Laf1

Laf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Laf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available