ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.43 0.68 0.62 0.41 0.23 0.15 0.2 0.12 0.15 0.12 0.07 0.53 0.47 0.44 0.14 0.12 0.12 0.29 0.18 0.28 0.37 0.31 0.31
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.05 0 0.12 0.14 0.27 0.59 0.47 0.69 0.58 0.77 0.49 0 0 0 0 0.06 0.07 0.06 0.07 0.07 0.07 0.07 0.07
Nucleus 0.12 0.18 0.21 0.23 0.38 0.55 0.52 0.55 0.54 0.58 0.62 0.74 0.34 0.41 0.45 0.76 0.76 0.79 0.25 0.35 0.25 0.18 0.18 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.51 0.09 0.07 0.14 0.08 0.06 0 0 0 0 0 0.15 0.15 0.13 0.14 0.14 0.08 0.29 0.33 0.29 0.25 0.3 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 1 2 0 0 2
Bud 2 2 0 0 0 2 8 4 11 10 9 8 0 0 0 0 0 1 3 1 1 3 10 5
Bud Neck 1 0 7 2 4 2 1 6 2 4 1 0 1 0 0 0 0 0 2 0 1 1 4 11
Bud Site 0 1 0 0 0 2 4 2 7 9 4 6 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 147 86 159 173 144 73 84 88 42 66 29 19 150 116 128 43 25 24 100 44 87 81 110 122
Endoplasmic Reticulum 1 0 1 0 1 0 0 1 0 0 0 0 3 1 0 14 10 13 2 1 1 2 1 2
Endosome 0 3 1 1 6 2 0 2 0 1 0 0 1 5 4 4 3 0 1 1 3 1 4 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 2 0 2 4 1 0 2 1 1
Mitochondria 145 11 10 32 47 88 327 202 244 256 190 141 5 2 1 7 12 14 19 18 21 16 24 25
Nucleus 35 37 48 64 131 178 286 237 191 255 154 216 96 100 129 240 156 161 84 84 78 40 62 66
Nuclear Periphery 0 0 0 0 0 0 1 0 0 3 1 0 0 1 0 0 0 1 0 1 0 0 0 1
Nucleolus 0 0 1 0 0 0 0 2 0 4 2 4 0 0 0 0 2 0 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 2 5 3 5 4 2 8 1 1 2 0 1 2 2 1 8 3 6 4 13 17
Vac/Vac Membrane 34 103 21 20 48 27 32 10 12 11 5 7 42 36 39 44 29 16 99 80 91 54 104 116
Unique Cell Count 296 201 234 277 347 323 551 433 352 440 247 290 281 246 289 317 205 203 341 244 310 220 352 391
Labelled Cell Count 365 243 250 295 386 377 748 558 511 627 396 402 300 263 304 358 239 233 341 244 310 220 352 391


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.5 4.8 4.9 4.7 4.2 3.8 4.3 3.6 3.8 3.6 4.2 5.1 5.5 5.3 6.7 6.5 6.1 4.8 5.0 5.3
Std Deviation (1e-4) 0.8 0.9 1.2 2.0 1.3 1.4 1.2 1.3 1.3 1.2 1.2 1.1 1.3 1.9 1.5 1.2 1.3 1.2 1.9 1.9 1.8
Intensity Change (Log2) – – – 0.01 -0.03 -0.22 -0.33 -0.17 -0.43 -0.33 -0.44 -0.21 0.08 0.19 0.12 0.47 0.43 0.34 0.01 0.06 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.8 1.7 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0199 0.0021 0.0043 0.002 0.0271 0.0049 0.0194 0.0004 0.0145 0.0737 0.0205 0.0035 0.0001 0.0001 0.0009 0.0074 0.0001 0.0026
Bud 0.0011 0.0055 0.0002 0.0001 0.002 0.0009 0.0063 0.0004 0.0004 0.0003 0.0137 0.0001 0 0.0001 0.0003 0.0026 0 0.0016
Bud Neck 0.0048 0.0005 0.0009 0.0005 0.0013 0.0027 0.0012 0.0007 0.0009 0.002 0.0036 0.0031 0.0004 0.0003 0.0004 0.0008 0.0003 0.0019
Bud Periphery 0.0019 0.0013 0.0003 0.0003 0.0071 0.0007 0.0025 0.0002 0.0006 0.0004 0.0039 0.0002 0 0.0001 0.0003 0.0082 0 0.0023
Bud Site 0.0066 0.0031 0.0006 0.0007 0.0024 0.0004 0.0034 0.0039 0.0032 0.0009 0.0067 0.0002 0.0002 0.0022 0.0025 0.0028 0.0001 0.0072
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0002 0 0 0.0001 0.0002 0.0002 0 0.0002
Cytoplasm 0.1012 0.1459 0.0618 0.0882 0.0885 0.0869 0.0937 0.1248 0.0934 0.1156 0.0692 0.0942 0.2477 0.1901 0.2345 0.1634 0.0209 0.1672
Cytoplasmic Foci 0.0169 0.0043 0.0147 0.008 0.0238 0.0079 0.028 0.0046 0.0047 0.0038 0.0415 0.0094 0.0028 0.0237 0.0118 0.0198 0.0002 0.0172
Eisosomes 0.0004 0.0001 0.0001 0.0006 0.0004 0.0001 0.0004 0.0001 0.0002 0.0003 0.0004 0 0 0 0 0.0005 0 0.0001
Endoplasmic Reticulum 0.0038 0.0027 0.0023 0.0018 0.0038 0.0015 0.005 0.0032 0.0043 0.0035 0.0055 0.0014 0.0048 0.0074 0.0142 0.0067 0.0003 0.0035
Endosome 0.0216 0.0033 0.0095 0.0102 0.0354 0.0078 0.0128 0.0023 0.006 0.0051 0.0244 0.0088 0.0043 0.0247 0.0193 0.0283 0.0005 0.0163
Golgi 0.0059 0.0008 0.0021 0.0013 0.0175 0.0056 0.0042 0.0001 0.0033 0.0032 0.0115 0.0078 0 0.001 0.0258 0.0018 0 0.0061
Lipid Particles 0.0187 0.001 0.0076 0.0107 0.0345 0.0036 0.0159 0.0004 0.0061 0.0026 0.0662 0.0084 0.0001 0.0021 0.0011 0.0039 0 0.0025
Mitochondria 0.0084 0.0028 0.002 0.0029 0.0291 0.0068 0.0142 0.0009 0.0082 0.0024 0.0057 0.0055 0.0003 0.0005 0.0026 0.0161 0.0005 0.0459
None 0.3224 0.3345 0.3353 0.3912 0.3566 0.2389 0.2836 0.3654 0.2808 0.3015 0.2274 0.1745 0.367 0.347 0.3505 0.3439 0.0059 0.1606
Nuclear Periphery 0.0154 0.0136 0.0212 0.0077 0.0186 0.0074 0.0268 0.0169 0.0291 0.0162 0.0611 0.0128 0.0201 0.0074 0.0239 0.0316 0.0097 0.0084
Nucleolus 0.0059 0.0067 0.006 0.0046 0.0052 0.0072 0.0079 0.0051 0.0073 0.0083 0.0058 0.0064 0.0026 0.0016 0.0041 0.0085 0.0046 0.0042
Nucleus 0.3885 0.4301 0.4786 0.4046 0.2286 0.576 0.4106 0.4103 0.4934 0.4352 0.3457 0.5849 0.32 0.3798 0.289 0.2024 0.9386 0.5308
Peroxisomes 0.0127 0.0077 0.0068 0.0043 0.0339 0.0055 0.0072 0.0003 0.0048 0.0023 0.0276 0.0068 0 0.0004 0.0023 0.0472 0 0.0042
Punctate Nuclear 0.0398 0.0303 0.0419 0.0581 0.0783 0.0332 0.0513 0.0576 0.0355 0.0196 0.0534 0.0706 0.0253 0.0026 0.0064 0.0312 0.018 0.0108
Vacuole 0.003 0.0032 0.0027 0.0012 0.003 0.0015 0.0032 0.002 0.0022 0.0022 0.0033 0.001 0.0037 0.008 0.0086 0.0617 0.0003 0.0053
Vacuole Periphery 0.001 0.0005 0.001 0.0006 0.0023 0.0005 0.0018 0.0003 0.001 0.0007 0.0025 0.0004 0.0005 0.0008 0.0014 0.0112 0.0001 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.4821 4.4552 5.9507 5.8207 7.1078 13.9468 4.2794 8.8169 9.3634 5.4582
Translational Efficiency 0.7801 1.3823 0.6537 1.0982 0.4799 0.4634 1.0434 0.8398 0.5766 0.4893

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
25 1336 1656 1851 1545 1722 180 1294 1570 3058 1836 3145

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 843.51 760.48 757.59 829.20 661.69 704.00 920.87 855.79 664.59 728.68 773.60 840.14
Standard Deviation 186.10 88.08 138.30 117.50 73.94 87.65 115.70 127.26 80.31 92.20 144.64 122.31
Intensity Change Log 2 — -0.149495 -0.154988 -0.024685 — 0.089420 0.476842 0.371101 — -0.039567 0.157183 0.162785

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002173 0.000361 0.000196 0.001580 0.000060 0.000276 0.000537 0.001013 0.000093 0.000313 0.000230 0.001347
Bud Neck 0.022093 0.017688 0.004271 0.014182 0.004178 0.014626 0.003631 0.016979 0.004464 0.015964 0.004209 0.015333
Bud Site 0.011829 0.005717 0.003613 0.015329 0.001468 0.004413 0.004937 0.027089 0.001633 0.004983 0.003743 0.020168
Cell Periphery 0.000524 0.000198 0.000169 0.000190 0.000170 0.000147 0.000089 0.000299 0.000176 0.000169 0.000161 0.000235
Cytoplasm 0.027098 0.239242* 0.114147* 0.109669* 0.307122* 0.253201* 0.026730 0.138286* 0.302663* 0.247102* 0.105577* 0.121443*
Cytoplasmic Foci 0.040647 0.100393 0.002634 0.001634 0.064374 0.064996 0.001213 0.005081 0.063997 0.080460 0.002495 0.003052
Eisosomes 0.000124 0.000078 0.000021 0.000031 0.000053 0.000056 0.000056 0.000039 0.000054 0.000065 0.000025 0.000034
Endoplasmic Reticulum 0.039621 0.002349 0.002926 0.006527 0.000784 0.001398 0.008732 0.006649 0.001402 0.001813 0.003495 0.006577
Endosome 0.025128 0.003998 0.000913 0.002881 0.001661 0.003162 0.000826 0.006515 0.002035 0.003527 0.000905 0.004377
Golgi 0.013320 0.001269 0.000058 0.001210 0.000507 0.001647 0.000042 0.003604 0.000711 0.001482 0.000056 0.002195
Lipid Particles 0.033068 0.006875 0.000615 0.000755 0.003737 0.003883 0.001232 0.000861 0.004204 0.005190 0.000675 0.000799
Mitochondria 0.023126 0.009279 0.001116 0.004755 0.004093 0.006724 0.000481 0.004162 0.004396 0.007840 0.001054 0.004511
Mitotic Spindle 0.008127 0.003591 0.003552 0.025546 0.001141 0.005804 0.003175 0.035997 0.001252 0.004837 0.003515 0.029846
None 0.016549 0.005466 0.013504 0.004616 0.005919 0.006393 0.003477 0.008890 0.006089 0.005988 0.012521 0.006374
Nuclear Periphery 0.004875 0.001921 0.001383 0.003892 0.001043 0.001183 0.001181 0.002475 0.001104 0.001505 0.001363 0.003309
Nuclear Periphery Foci 0.025166 0.000696 0.000493 0.002381 0.000196 0.000403 0.000406 0.002029 0.000594 0.000531 0.000485 0.002237
Nucleolus 0.038223 0.003299 0.001447 0.001860 0.001286 0.003035 0.001028 0.001486 0.001874 0.003151 0.001406 0.001706
Nucleus 0.585313* 0.567642* 0.839093* 0.769218* 0.590792* 0.603353* 0.919739* 0.701072* 0.590705* 0.587751* 0.847000* 0.741180*
Peroxisomes 0.008295 0.009366 0.000148 0.000417 0.003048 0.007159 0.000614 0.000670 0.003131 0.008123 0.000193 0.000521
Vacuole 0.060742 0.019783 0.009498 0.030671 0.008039 0.016787 0.021773 0.032912 0.008878 0.018096 0.010701 0.031593
Vacuole Periphery 0.013958 0.000791 0.000203 0.002654 0.000329 0.001354 0.000101 0.003891 0.000546 0.001108 0.000193 0.003163

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.40 2.56 1.30 -1.70 -2.00 -3.48 -7.33 -9.46 -6.01 -0.38 -4.72 -6.81 -3.84 -2.86 -2.88
Bud Neck 0.34 1.36 0.67 2.03 -7.44 -7.08 -1.87 -9.35 -0.65 -5.54 -10.00 -1.63 -11.61 1.01 -10.19
Bud Site 1.12 1.44 0.20 -3.37 -4.69 -3.71 -1.39 -7.40 -5.71 -3.87 -4.97 -2.64 -9.88 -6.62 -6.89
Cell Periphery 1.29 1.45 1.47 1.67 0.21 1.13 2.20 -0.87 -1.83 -2.67 0.22 0.77 0.14 -0.10 -0.76
Cytoplasm -14.63 -5.78 -5.37 19.03 1.26 6.14 35.11 25.76 20.72 -12.27 7.17 25.89 28.03 28.51 -0.25
Cytoplasmic Foci -2.90 1.96 2.03 22.39 3.39 -0.32 19.35 18.80 21.38 -2.17 -4.29 19.01 19.31 30.82 1.77
Eisosomes 1.70 3.84 3.25 9.90 -7.60 -0.90 -3.02 0.58 1.88 3.44 -3.20 6.12 2.52 8.82 -7.22
Endoplasmic Reticulum 2.58 2.49 2.18 -15.30 -11.12 -4.54 -9.06 -20.00 -18.47 2.45 -1.63 -9.02 -19.16 -24.26 -11.59
Endosome 2.37 2.73 2.60 5.21 -4.56 -3.84 1.04 -2.22 0.04 -2.78 -4.25 3.16 -1.25 2.89 -4.85
Golgi 1.68 1.84 1.77 2.07 -2.92 -2.83 2.69 -1.77 0.53 -2.88 -2.42 3.27 -0.60 1.82 -3.99
Lipid Particles 1.60 1.99 1.98 12.35 -2.27 -0.52 5.02 7.43 10.57 1.94 -2.07 7.54 7.27 16.21 -1.97
Mitochondria 1.57 2.52 2.24 3.55 -4.20 -1.71 2.99 0.96 3.51 -6.24 -2.38 2.85 0.92 4.81 -5.48
Mitotic Spindle 1.15 0.87 -2.31 -6.20 -5.58 -3.33 -0.82 -7.25 -5.19 -3.82 -3.74 -3.05 -10.14 -7.69 -7.64
None 0.85 0.36 0.92 1.24 5.55 -0.41 3.17 -0.40 0.13 -4.47 0.14 -3.54 1.12 1.19 4.82
Nuclear Periphery 1.40 1.51 -0.11 -10.58 -13.25 -1.94 -5.21 -11.63 -10.66 -6.78 -4.25 -7.37 -19.02 -15.84 -15.39
Nuclear Periphery Foci 2.06 2.07 1.98 -1.90 -2.24 -3.70 -3.00 -3.87 -3.21 -2.46 0.11 0.01 -2.83 -3.40 -3.21
Nucleolus 2.78 2.94 2.94 3.92 0.23 -4.36 1.05 -0.47 4.05 -1.39 -3.65 0.94 1.37 5.41 0.29
Nucleus 0.18 -3.36 -2.51 -20.07 7.88 -0.93 -20.50 -14.08 -13.40 9.91 0.59 -26.85 -18.79 -23.97 11.52
Peroxisomes -0.41 2.72 2.69 10.25 -1.98 -3.46 4.01 4.49 6.62 0.67 -5.56 4.97 4.79 11.28 -2.13
Vacuole 1.87 2.21 0.45 -10.30 -12.76 -7.61 -5.06 -14.81 -11.53 -2.07 -9.30 -5.13 -20.60 -15.60 -15.21
Vacuole Periphery 2.70 2.83 2.58 -1.70 -3.58 -2.42 2.52 -2.16 -0.28 -2.54 -2.11 2.92 -2.79 -0.89 -4.18
ORF
Human Ortholog
Description Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
Localization
Cell Percentages nucleus (50%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

YMR114C

YMR114C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr114c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available