Standard name
Human Ortholog
Description V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0.06 0 – 0.09 0.11 0.1 0.12 0.1 0.05 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.05 0.19 0.09 0 – 0.07 0.08 0.1 0.14 0.08 0.15 0.26 0.24 0.37 0.29 0.27 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.06 0.08 0.09 – 0.12 0.09 0.13 0.14 0.16 0 0.05 0.05 0.15 0.18 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0.9 0.96 0.88 0.79 0.86 0.78 – 0.61 0.66 0.6 0.53 0.56 0.89 0.82 0.84 0 0 0 0.93 0.95 0.93 0.93 0.9 0.9
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0.26 0.37 0.46 0 0 0 0 0 0
Mitochondria 0 0 0 0.19 0.06 0.13 – 0.12 0.23 0.14 0.29 0.23 0 0 0 0 0.09 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.06 0.07 0.08 0.12 – 0 0.1 0.05 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.06 0 0 0 – 0.09 0.06 0.11 0 0.07 0 0 0 0.3 0.22 0.25 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 4 9 12 14 11 – 30 38 41 33 24 22 19 11 2 2 2 12 1 2 2 10 4
Bud 5 0 8 0 3 2 – 7 2 4 5 5 0 0 0 0 1 1 4 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 24 12 15 39 23 12 – 22 29 42 39 20 64 92 82 17 20 18 5 3 2 2 4 8
Cytoplasm 68 11 13 12 21 25 – 40 33 52 38 39 13 19 19 7 12 9 19 2 1 3 6 7
Endoplasmic Reticulum 817 251 239 166 217 228 – 206 230 247 146 135 371 291 292 0 1 0 907 252 265 249 350 425
Endosome 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Golgi 2 2 0 1 6 1 – 2 1 3 2 1 20 5 4 12 25 31 1 0 1 2 1 2
Mitochondria 4 0 11 40 15 39 – 40 79 58 82 54 0 5 0 0 6 0 4 1 3 2 4 3
Nucleus 1 0 0 0 0 1 – 3 1 2 0 1 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 5 1 17 15 20 35 – 10 35 21 10 12 9 17 12 0 0 0 5 1 0 1 2 1
Nucleolus 0 0 0 0 0 0 – 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 41 2 17 2 6 7 – 31 21 44 10 17 6 0 3 14 15 17 7 1 2 3 5 11
Unique Cell Count 905 261 273 210 251 291 336 350 411 278 240 417 355 347 46 68 67 974 267 284 270 391 473
Labelled Cell Count 983 283 329 287 325 362 392 470 514 366 308 506 448 423 52 83 78 974 267 284 270 391 473


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.6 29.7 14.6 18.2 16.6 13.9 – 13.8 12.8 13.4 12.5 12.5 24.5 24.6 22.5 36.8 35.5 37.1 25.3 25.0 25.5
Std Deviation (1e-4) 4.9 5.3 3.1 3.4 3.1 2.5 – 3.0 2.5 2.4 2.4 2.5 4.7 4.4 4.0 10.0 8.7 9.1 4.9 4.8 4.7
Intensity Change (Log2) – – – 0.32 0.19 -0.07 – -0.07 -0.19 -0.13 -0.22 -0.23 0.75 0.76 0.63 1.34 1.28 1.35 0.8 0.78 0.81

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP700010203040WT3HU80HU120HU160010203040WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3010203040WT1AF100AF140AF180010203040
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 1.3 0.3 – 2.8 3.5 3.3 3.8 3.1 1.2 1.2 -0.1 0 0 0
Bud 0 -1.4 0 – -0.7 0 0 -0.9 -0.6 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4.5 1.6 -0.8 – 0.5 1.3 2.2 3.4 1.3 4.0 6.7 6.2 0 5.8 5.3
Cytoplasm 0.5 1.7 1.8 – 3.1 2.2 3.4 3.6 4.3 -1.1 0.3 0.4 0 0 0
Endoplasmic Reticulum -2.5 -0.4 -2.9 – -7.3 -6.3 -7.8 -9.0 -8.0 0.6 -1.9 -1.2 -12.7 -13.9 -14.1
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 3.7 0 0 0 0 11.8
Mitochondria 5.3 1.0 3.9 – 3.5 6.5 4.3 8.0 6.3 0 -2.1 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.4 0.8 2.4 – -1.9 1.7 -0.6 -1.4 -0.6 -2.7 -0.8 -1.6 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 -2.1 -2.2 – 1.4 -0.1 2.0 -1.4 0.4 -3.4 -4.8 -3.8 0 4.0 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.2596 25.1018 24.7974 19.0763 21.763 24.7272 19.6048 22.3903 19.8102 20.9016 18.2723 21.2876 23.8156 26.9327 25.2773 24.5461 21.5176 25.0496
Actin 0.0079 0.0011 0.0105 0.0073 0.0047 0.0012 0.0277 0.0003 0.0049 0.0007 0.0024 0.0009 0.0312 0.0019 0.0143 0.0014 0.0176 0.0014
Bud 0.0003 0.0001 0.0002 0.0004 0.0004 0 0.0003 0 0.0004 0 0.0003 0 0.0004 0.0001 0.0003 0.0002 0.0008 0
Bud Neck 0.0008 0.0003 0.0006 0.002 0.0018 0.0009 0.0005 0.0002 0.0004 0.0002 0.0003 0.0012 0.0033 0.0005 0.0005 0.0001 0.004 0.001
Bud Periphery 0.0005 0.0002 0.0002 0.0008 0.0006 0.0002 0.0007 0.0001 0.0006 0 0.0007 0.0001 0.0006 0.0002 0.0003 0.0004 0.0015 0.0001
Bud Site 0.0025 0.0005 0.0009 0.0017 0.0073 0.0001 0.0009 0.0003 0.0008 0 0.0009 0.0001 0.0058 0.0026 0.0055 0.0005 0.001 0
Cell Periphery 0.0046 0.0089 0.0027 0.0035 0.0032 0.001 0.0062 0.0076 0.0048 0.001 0.003 0.0011 0.0023 0.0113 0.0052 0.0006 0.0146 0.0009
Cytoplasm 0.0099 0.0269 0.024 0.0148 0.0034 0.0057 0.0087 0.0131 0.012 0.0016 0.0014 0.0081 0.0155 0.033 0.0246 0.0063 0.0041 0.0049
Cytoplasmic Foci 0.0105 0.0004 0.0024 0.0044 0.004 0.0016 0.0095 0.0002 0.014 0.0004 0.0068 0.0014 0.0341 0.0003 0.0018 0.0026 0.0118 0
Eisosomes 0.0009 0.0001 0.0001 0.0003 0.0004 0 0.0017 0 0.0002 0 0.0006 0.0001 0.0002 0 0.0002 0.0001 0.0004 0
Endoplasmic Reticulum 0.7191 0.9276 0.9129 0.831 0.8935 0.9404 0.7878 0.9518 0.8665 0.9691 0.8251 0.9533 0.7372 0.9211 0.9275 0.8674 0.6946 0.948
Endosome 0.0154 0.0005 0.0102 0.0237 0.0188 0.0042 0.0275 0.0003 0.0215 0.0076 0.0617 0.0027 0.0363 0.0005 0.004 0.0467 0.0208 0.001
Golgi 0.0035 0.0003 0.0015 0.0065 0.0078 0.0024 0.009 0.0003 0.0094 0.0018 0.0169 0.0007 0.0105 0.0003 0.0015 0.0055 0.0347 0.0002
Lipid Particles 0.0135 0.0101 0.0027 0.0141 0.0118 0.002 0.0124 0.0116 0.017 0.0011 0.026 0.0018 0.0173 0.0131 0.0013 0.0082 0.0842 0.0002
Mitochondria 0.0052 0.0006 0.0037 0.0328 0.0074 0.0025 0.0152 0 0.0117 0.0001 0.0177 0.0055 0.0023 0 0.0014 0.0163 0.0472 0.0001
None 0.0131 0.0001 0.0004 0.0023 0.0016 0.0001 0.0041 0 0.0045 0.0002 0.0007 0.0003 0.0047 0 0.0033 0.0016 0.0003 0
Nuclear Periphery 0.1411 0.0139 0.0167 0.0276 0.0193 0.029 0.0589 0.0095 0.0115 0.0137 0.0133 0.0157 0.0532 0.011 0.0042 0.025 0.01 0.0355
Nucleolus 0.0046 0 0.0003 0.0023 0.0009 0 0.0006 0 0.0002 0 0.0007 0.0001 0.0008 0 0 0.0004 0.0001 0
Nucleus 0.0168 0.0009 0.0014 0.0056 0.0013 0.0024 0.0065 0.0007 0.0006 0.0003 0.0022 0.0009 0.0037 0.0006 0.0003 0.0028 0.0006 0.0022
Peroxisomes 0.0047 0 0.0012 0.0014 0.0018 0.0012 0.0022 0 0.0042 0 0.0017 0.0003 0.0213 0 0.0002 0.0014 0.0412 0
Punctate Nuclear 0.0101 0.0001 0.0005 0.0027 0.0004 0.0001 0.0059 0 0.0004 0.0001 0.0038 0.0028 0.0054 0.0001 0.0001 0.0009 0.0005 0
Vacuole 0.0103 0.0059 0.0042 0.0093 0.008 0.0031 0.0097 0.0036 0.0121 0.0017 0.0077 0.0023 0.0114 0.0032 0.0031 0.0068 0.008 0.0036
Vacuole Periphery 0.005 0.0014 0.0027 0.0056 0.0018 0.0018 0.004 0.0003 0.0024 0.0005 0.0061 0.0005 0.0024 0.0002 0.0003 0.0049 0.002 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.7467 85.5394 67.441 83.0123 91.1094 80.6922 72.9749 90.2974 70.4637 82.35
Translational Efficiency 1.5856 1.6338 1.8199 1.1613 1.1856 1.3238 1.5551 0.9466 1.3708 1.2735

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
183 1686 162 144 1248 1779 1421 1356 1431 3465 1583 1500

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1563.85 1981.96 2256.28 2756.88 1746.42 1930.88 2069.27 2492.30 1723.07 1955.73 2088.41 2517.70
Standard Deviation 248.68 390.79 379.64 377.91 328.60 373.76 491.91 490.12 325.26 382.99 484.95 486.77
Intensity Change Log 2 — 0.341826 0.528844 0.817934 — 0.144858 0.244721 0.513077 — 0.241267 0.385935 0.665143

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000892 0.000784 0.000587 0.000339 0.000291 0.000438 0.000510 0.001482 0.000368 0.000606 0.000518 0.001372
Bud Neck 0.000309 0.000276 0.012105 0.008136 0.000178 0.000368 0.001377 0.002298 0.000195 0.000323 0.002475 0.002859
Bud Site 0.000640 0.002176 0.011167 0.001570 0.000632 0.001863 0.004057 0.005065 0.000633 0.002015 0.004785 0.004730
Cell Periphery 0.001501 0.004243 0.012728 0.024144 0.001811 0.003057 0.008110 0.018051 0.001772 0.003634 0.008583 0.018636
Cytoplasm 0.000187 0.001702 0.003438 0.000605 0.000339 0.000629 0.010255 0.001860 0.000320 0.001151 0.009557 0.001739
Cytoplasmic Foci 0.000326 0.000736 0.000364 0.000049 0.000193 0.000587 0.001235 0.000547 0.000210 0.000659 0.001146 0.000499
Eisosomes 0.000062 0.000076 0.000718 0.001603 0.000032 0.000037 0.000073 0.000237 0.000036 0.000056 0.000139 0.000368
Endoplasmic Reticulum 0.972308 0.975511 0.648118 0.885249 0.981979 0.977560 0.905433 0.919220 0.980742 0.976563 0.879100 0.915958
Endosome 0.005253 0.003622 0.026988 0.006967 0.002980 0.003548 0.009141 0.006526 0.003271 0.003584 0.010968 0.006568
Golgi 0.002458 0.002029 0.004878 0.007053 0.002608 0.002150 0.009857 0.008446 0.002589 0.002091 0.009347 0.008312
Lipid Particles 0.004767 0.001549 0.014429 0.020530 0.001154 0.001060 0.010179 0.009900 0.001616 0.001298 0.010614 0.010920
Mitochondria 0.000692 0.000400 0.000303 0.000153 0.000756 0.000702 0.000340 0.001519 0.000748 0.000555 0.000336 0.001388
Mitotic Spindle 0.000104 0.000122 0.005043 0.000219 0.000027 0.000119 0.000174 0.001267 0.000037 0.000120 0.000672 0.001167
None 0.000063 0.000447 0.000077 0.000021 0.000259 0.000567 0.002114 0.001036 0.000234 0.000508 0.001905 0.000939
Nuclear Periphery 0.001390 0.000726 0.074405 0.005367 0.000941 0.000670 0.001821 0.002544 0.000998 0.000697 0.009249 0.002815
Nuclear Periphery Foci 0.003225 0.001509 0.139821 0.012115 0.001848 0.001723 0.016962 0.003991 0.002024 0.001619 0.029535 0.004771
Nucleolus 0.000012 0.000009 0.000028 0.000008 0.000006 0.000019 0.000025 0.000023 0.000006 0.000014 0.000025 0.000022
Nucleus 0.000142 0.000175 0.000503 0.000143 0.000049 0.000114 0.000377 0.000186 0.000061 0.000144 0.000390 0.000182
Peroxisomes 0.000073 0.000109 0.000018 0.000002 0.000014 0.000053 0.000023 0.000057 0.000021 0.000080 0.000023 0.000052
Vacuole 0.003026 0.002623 0.041520 0.023551 0.002025 0.002997 0.013944 0.012033 0.002153 0.002815 0.016766 0.013139
Vacuole Periphery 0.002568 0.001178 0.002761 0.002175 0.001878 0.001737 0.003994 0.003713 0.001966 0.001465 0.003868 0.003565

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.14 0.40 0.51 0.68 0.21 -0.87 -1.04 -2.72 -2.44 -2.21 -1.16 -0.76 -2.56 -2.04 -2.19
Bud Neck 0.29 -4.99 -2.33 -2.34 0.70 -1.27 -3.37 -3.90 -3.51 -1.23 -1.28 -5.51 -4.50 -4.30 -0.50
Bud Site -2.17 -1.61 -1.87 -0.02 1.36 -2.25 -4.22 -4.61 -3.20 -1.02 -2.94 -4.25 -4.75 -3.13 -0.22
Cell Periphery -3.49 -2.55 -4.53 -3.94 -1.72 -2.82 -8.15 -9.47 -8.59 -5.30 -4.07 -8.26 -10.39 -9.01 -5.53
Cytoplasm -2.76 -2.00 -0.97 -0.25 0.42 -1.34 -7.06 -2.86 -2.15 5.52 -2.69 -7.25 -3.04 -1.38 5.50
Cytoplasmic Foci -1.29 -0.30 2.42 2.26 3.83 -1.99 -5.16 -2.70 0.16 2.93 -2.49 -5.15 -2.45 0.74 3.05
Eisosomes -0.62 -0.99 -2.18 -2.16 -0.95 -0.99 -4.25 -5.76 -5.60 -4.48 -2.50 -1.54 -4.41 -4.13 -2.30
Endoplasmic Reticulum -0.44 12.24 4.73 5.21 -7.26 1.46 12.54 11.40 10.57 -1.72 1.59 15.81 12.25 11.88 -4.48
Endosome 1.24 -7.45 -1.32 -3.15 6.76 -1.49 -9.21 -6.15 -4.97 3.23 -1.01 -11.19 -6.08 -5.75 5.46
Golgi 0.55 -1.80 -2.63 -2.98 -1.01 0.53 -5.16 -4.53 -5.56 0.94 0.67 -5.36 -4.91 -6.14 0.78
Lipid Particles 1.04 -2.28 -2.15 -2.79 -0.78 0.14 -5.98 -7.34 -7.70 0.17 0.53 -6.26 -7.10 -7.82 -0.14
Mitochondria 0.99 1.29 0.99 0.28 -0.09 0.08 0.84 -1.44 -2.22 -3.50 0.44 0.95 -1.40 -2.73 -3.50
Mitotic Spindle -0.15 -1.11 -1.16 -1.11 1.05 -1.68 -3.54 -2.10 -1.93 -1.83 -1.86 -1.39 -2.15 -1.99 -0.66
None -3.54 -0.25 0.96 3.79 0.86 -1.61 -2.25 -1.77 -0.97 1.16 -2.12 -2.26 -1.78 -1.05 1.16
Nuclear Periphery 1.51 -7.56 -4.32 -5.65 7.12 0.90 -2.91 -4.41 -6.91 -2.39 1.14 -7.12 -5.40 -8.42 5.55
Nuclear Periphery Foci 1.14 -8.61 -1.95 -2.46 7.71 0.06 -7.42 -2.30 -2.55 6.02 0.61 -10.52 -2.88 -3.59 9.06
Nucleolus 0.52 -1.60 0.53 -0.06 2.45 -1.30 -3.73 -3.47 -0.36 0.22 -1.30 -3.93 -3.31 -1.08 0.50
Nucleus -0.21 -2.71 -0.74 -0.66 0.98 -1.57 -5.03 -3.87 -1.58 2.63 -1.86 -5.42 -3.59 -1.25 2.81
Peroxisomes -0.50 0.96 1.26 2.50 1.75 -2.35 -2.08 -4.26 -0.14 -3.18 -2.51 -0.13 -2.62 1.19 -3.02
Vacuole 0.31 -7.95 -3.65 -3.79 2.11 -1.34 -8.81 -7.35 -6.58 0.60 -1.14 -10.97 -8.17 -7.80 1.45
Vacuole Periphery 1.55 -0.10 0.40 -1.37 0.61 0.23 -2.43 -2.13 -2.43 0.29 0.99 -2.41 -2.04 -3.11 0.34
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Pkr1

Pkr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pkr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available