Standard name
Human Ortholog
Description Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.35 0.16 0.22 0.12 0.14 0.1 0.09 0.1 0 0.08 0.06 0.34 0.35 0.24 0.3 0.29 0.31 0.16 0.14 0.14 0.18 0.18 0.21
Bud 0.22 0.33 0.27 0.24 0.24 0.21 0.21 0.18 0.17 0.14 0.19 0.14 0.19 0.22 0.23 0.22 0.23 0.22 0.49 0.52 0.35 0.31 0.14 0.08
Bud Neck 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0.22 0.25 0.29 0.25 0.18 0.1
Bud Site 0.31 0.16 0.23 0.19 0.15 0.18 0.12 0.07 0.09 0.12 0.07 0.07 0.19 0.05 0 0.16 0.15 0.13 – – – – – –
Cell Periphery 0.05 0.05 0 0.08 0 0 0 0 0.05 0.05 0 0.09 0.13 0.22 0.3 0.07 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.17 0.21 0.24 0.41 0.35 0.39 0.37 0.38 0.36 0.39 0.38 0.36 0.34 0.4 0.49 0.24 0.22 0.25 0 0 0.05 0.11 0.32 0.32
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.11
Endosome 0.07 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.33 0.2 0.23 0.07 0.27 0.27 0.36 0.5 0.46 0.56 0.5 0.1 0 0.14 0.09 0.07 0.11 0 0 0.05 0.05 0 0
Nucleus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.09 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.15 0.12 0.1 0.11 0.12 0.09 0.07 0 0 0.05 0 0 0.16 0.11 0 0.11 0.11 0.1 0 0 0 0 0 0
SpindlePole 0.22 0 0.18 0.1 0.19 0.09 0.1 0.1 0.05 0.09 0 0.06 0.08 0.07 0 0.16 0.19 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 71 15 29 61 45 54 55 61 34 16 21 19 49 115 84 103 69 96 45 10 28 27 42 73
Bud 52 14 50 66 90 83 118 124 60 56 48 44 27 73 81 76 56 69 135 39 72 46 32 25
Bud Neck 3 0 12 4 6 8 10 14 5 5 2 4 0 2 0 6 30 4 61 18 59 37 41 35
Bud Site 74 7 43 52 57 69 68 45 30 49 18 21 27 17 8 54 35 40 – – – – – –
Cell Periphery 13 2 5 23 14 15 23 17 18 18 6 27 19 73 107 25 18 25 3 1 3 2 3 12
Cytoplasm 40 9 44 114 135 149 207 261 125 152 97 114 49 131 173 83 52 77 7 2 10 16 74 109
Endoplasmic Reticulum 1 2 0 4 5 6 12 16 12 7 8 3 3 14 14 7 6 18 5 0 0 0 8 37
Endosome 17 1 2 1 26 7 11 11 3 3 1 1 4 11 4 12 6 11 2 0 2 3 8 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0 0 0 4
Mitochondria 8 14 37 63 28 104 152 245 173 181 140 158 14 8 49 31 17 34 6 0 9 7 8 13
Nucleus 19 1 8 5 15 5 16 12 5 7 5 3 0 2 5 32 26 28 0 0 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 1 3 0 2 2 1 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 9 1 4 3 6 3 13 23 8 4 5 6 1 1 0 10 7 6 0 0 0 0 0 0
Peroxisomes 37 5 18 31 47 33 40 22 14 19 3 13 23 35 9 39 27 30 1 0 0 0 0 1
SpindlePole 53 0 33 29 74 36 54 68 18 35 10 18 11 22 7 54 46 45 5 0 7 1 0 6
Vac/Vac Membrane 1 0 3 0 7 7 15 22 8 8 8 11 1 2 3 9 8 7 1 0 5 3 8 8
Unique Cell Count 239 43 184 279 381 387 554 684 348 393 252 313 143 325 353 348 240 313 279 76 207 150 233 343
Labelled Cell Count 398 71 288 457 555 579 795 944 513 562 374 443 228 506 544 543 406 492 279 76 207 150 233 343


Ambiguous, Bud, Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 5.5 4.6 4.8 5.4 4.7 4.5 4.6 4.3 4.2 4.1 4.1 6.4 6.6 5.7 7.9 9.0 9.2 6.5 6.2 7.3
Std Deviation (1e-4) 1.7 1.0 0.8 1.2 1.7 1.0 1.1 1.1 0.9 1.0 1.1 1.2 1.5 1.4 1.3 2.1 2.5 2.6 1.2 1.3 1.3
Intensity Change (Log2) – – – 0.05 0.21 0.04 -0.03 0.01 -0.1 -0.14 -0.16 -0.18 0.48 0.52 0.3 0.78 0.96 0.99 0.5 0.42 0.66

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.3 0 0 0 0 0 0 0 3.9 4.7 2.2 3.5 3.1 3.7
Bud 0 -0.9 0 0 0 0 0 0 0 -1.8 -1.2 -1.1 -1.4 -0.9 -1.3
Bud Neck 0 -3.1 0 0 0 0 0 0 0 -3.1 -3.9 0 -2.9 2.0 -3.2
Bud Site 0 -2.5 0 0 0 0 0 0 0 -1.0 -6.1 -7.9 -2.2 -2.3 -3.1
Cell Periphery 0 0.6 0 0 0 0 0 0 0 3.6 5.9 7.5 2.1 2.2 2.4
Cytoplasm 3.8 2.8 3.4 3.3 3.6 2.8 3.5 3.2 2.9 2.1 3.7 5.6 0 -0.5 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 2.9 0 0 0 3.3
Endosome 0 2.9 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -4.5 0 0 0 0 0 0 0 -2.6 -6.7 -1.9 -3.7 -4.0 -2.8
Nucleus -1.6 -0.2 0 -1.0 0 0 0 -1.4 0 0 0 0 2.0 2.4 1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.9 0 0 0 0 0 0 0 1.7 0.4 -3.6 0.5 0.5 -0.1
SpindlePole 0 0.4 0 0 0 0 0 0 0 -2.7 -3.9 -6.7 -0.7 0.3 -1.1
Vacuole 0 0 0 0 1.1 0 0 0 1.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7076 2.4441 2.1662 1.785 2.3391 2.8213 3.9488 3.9752 3.6986 3.7956 4.2228 4.4374 2.1776 2.5239 2.006 2.2995 2.0663 2.4592
Actin 0.0982 0.0191 0.0249 0.0119 0.1151 0.043 0.0855 0.0138 0.011 0.0714 0.0859 0.0525 0.0645 0.041 0.0329 0.0572 0.2616 0.0698
Bud 0.0579 0.0645 0.3441 0.1432 0.0515 0.015 0.0592 0.0884 0.4191 0.0996 0.0969 0.0228 0.0711 0.0396 0.3084 0.0924 0.05 0.0193
Bud Neck 0.0928 0.0212 0.0098 0.0298 0.0674 0.7269 0.0722 0.0175 0.0035 0.0017 0.0532 0.6703 0.0827 0.0244 0.0018 0.0116 0.0116 0.6923
Bud Periphery 0.1391 0.0526 0.4569 0.594 0.2466 0.0507 0.0919 0.0766 0.438 0.5899 0.4584 0.0482 0.1326 0.0531 0.4957 0.5733 0.307 0.0674
Bud Site 0.2807 0.6571 0.0949 0.0483 0.1115 0.0234 0.3221 0.5737 0.0659 0.0126 0.0226 0.0263 0.3311 0.5872 0.0751 0.0421 0.0949 0.0316
Cell Periphery 0.0275 0.0129 0.0067 0.0203 0.0206 0.0048 0.0202 0.0092 0.0031 0.0165 0.0143 0.0047 0.034 0.0209 0.0049 0.0285 0.0055 0.0098
Cytoplasm 0.0638 0.1178 0.0091 0.06 0.1659 0.0694 0.0967 0.1663 0.0184 0.1008 0.1298 0.0963 0.1495 0.1153 0.022 0.0802 0.0813 0.0693
Cytoplasmic Foci 0.0585 0.0049 0.0097 0.0083 0.0409 0.033 0.0679 0.0119 0.0088 0.0103 0.0832 0.03 0.0328 0.0048 0.0021 0.0047 0.0067 0.0239
Eisosomes 0.0017 0.0008 0.0002 0.0003 0.0008 0.0001 0.0009 0.0002 0.0001 0.0006 0.0007 0.0002 0.0013 0.0024 0.0004 0.0013 0.0013 0.0002
Endoplasmic Reticulum 0.0117 0.0009 0.0002 0.0017 0.0045 0.0005 0.0114 0.0037 0.0005 0.0074 0.0177 0.0028 0.0052 0.0017 0.0011 0.0038 0.0115 0.0003
Endosome 0.0308 0.0004 0.0005 0.0076 0.0246 0.0085 0.0423 0.0018 0.0043 0.0142 0.0037 0.0134 0.0093 0.0007 0.0019 0.0028 0.008 0.0008
Golgi 0.0104 0.0004 0.0011 0.0046 0.0192 0.0044 0.0205 0.0011 0.0027 0.0125 0.0023 0.0086 0.0045 0.0006 0.0013 0.0016 0.0089 0.0014
Lipid Particles 0.0192 0.0003 0.0015 0.0042 0.0291 0.0022 0.0209 0.0008 0.0022 0.0087 0.0037 0.0092 0.0154 0.0015 0.0012 0.0058 0.0047 0.0003
Mitochondria 0.0198 0.0004 0.0065 0.0329 0.0499 0.001 0.0243 0.0005 0.0094 0.0312 0.0009 0.0042 0.0036 0.0006 0.0026 0.0096 0.0041 0.0003
None 0.016 0.0364 0.0168 0.0043 0.0025 0.0043 0.0156 0.0257 0.0062 0.0067 0.0156 0.0027 0.029 0.0901 0.0374 0.0419 0.1316 0.0066
Nuclear Periphery 0.0084 0.0002 0.0001 0.0004 0.0158 0.001 0.0085 0.0006 0.0003 0.0012 0.0014 0.001 0.0021 0.0003 0.0006 0.0007 0.003 0
Nucleolus 0.007 0.002 0.0005 0.0056 0.0021 0.0004 0.0008 0.0004 0.0003 0.0002 0.0009 0.0004 0.0007 0.0081 0.0004 0.0005 0.0009 0.0002
Nucleus 0.0056 0.002 0.0005 0.0026 0.002 0.0007 0.0101 0.0016 0.0006 0.0006 0.0009 0.0007 0.0022 0.0023 0.0006 0.0017 0.0012 0.0004
Peroxisomes 0.0241 0.0008 0.0144 0.0089 0.0172 0.0061 0.0149 0.0019 0.0028 0.0085 0.0015 0.0026 0.0192 0.0008 0.0079 0.0287 0.0019 0.0025
Punctate Nuclear 0.0068 0.0005 0.0002 0.0005 0.0036 0.0012 0.0059 0.0009 0.0003 0.0004 0.0032 0.0009 0.0036 0.0008 0.0004 0.0005 0.0015 0.0004
Vacuole 0.0178 0.0049 0.0013 0.0097 0.0052 0.003 0.0063 0.0034 0.0022 0.004 0.003 0.0017 0.0051 0.0035 0.0012 0.0102 0.0022 0.0026
Vacuole Periphery 0.0022 0.0001 0.0001 0.0009 0.0039 0.0003 0.0019 0.0002 0.0005 0.0009 0.0004 0.0005 0.0003 0.0002 0.0002 0.0006 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.7146 15.6734 10.5965 17.572 13.1342 16.355 14.9669 18.6583 15.943 17.1513
Translational Efficiency 1.7176 1.1768 1.8003 1.0685 1.2629 1.2725 1.1575 0.9534 1.3701 1.1899

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1730 1063 434 936 1463 1844 176 377 3193 2907 610 1313

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 702.04 778.26 1150.90 964.32 682.65 808.72 1089.86 943.31 693.16 797.58 1133.29 958.29
Standard Deviation 90.29 131.22 136.81 125.17 123.13 111.79 110.98 124.97 107.04 120.16 132.80 125.48
Intensity Change Log 2 — 0.148699 0.713137 0.457959 — 0.244494 0.674925 0.466586 — 0.196721 0.694425 0.462218

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010781 0.009909 0.058662 0.011404 0.014590 0.007379 0.015799 0.006269 0.012526 0.008304 0.046295 0.009930
Bud Neck 0.153173 0.105316 0.047328 0.052160 0.175386 0.175087 0.064149 0.061136 0.163351 0.149573 0.052181 0.054737
Bud Site 0.576833 0.543800 0.673186 0.509523 0.418334 0.636137 0.652180 0.476714 0.504210 0.602373 0.667126 0.500102
Cell Periphery 0.010144 0.003329 0.002735 0.001041 0.015836 0.009231 0.002389 0.000820 0.012752 0.007073 0.002635 0.000978
Cytoplasm 0.153250 0.154571 0.106428 0.247800 0.174101 0.115136 0.067392 0.209431 0.162804 0.129556 0.095165 0.236783
Cytoplasmic Foci 0.034411 0.075279 0.030288 0.030261 0.076303 0.015204 0.023253 0.034721 0.053605 0.037172 0.028259 0.031542
Eisosomes 0.001398 0.000566 0.000389 0.000256 0.004813 0.000778 0.000320 0.000194 0.002963 0.000701 0.000369 0.000238
Endoplasmic Reticulum 0.001430 0.001696 0.004357 0.000574 0.002922 0.000256 0.005706 0.000743 0.002114 0.000783 0.004746 0.000623
Endosome 0.010374 0.028211 0.003702 0.009452 0.019438 0.006412 0.005194 0.006621 0.014527 0.014383 0.004133 0.008639
Golgi 0.005483 0.010473 0.001214 0.005979 0.010902 0.003698 0.000497 0.000563 0.007966 0.006175 0.001007 0.004424
Lipid Particles 0.003124 0.003944 0.001654 0.000616 0.017348 0.000502 0.002344 0.000960 0.009641 0.001761 0.001853 0.000715
Mitochondria 0.007036 0.008067 0.000182 0.003738 0.010968 0.006464 0.000312 0.002207 0.008838 0.007050 0.000219 0.003298
Mitotic Spindle 0.000718 0.002119 0.000676 0.007699 0.001037 0.003458 0.004283 0.003097 0.000864 0.002969 0.001717 0.006378
None 0.002411 0.001884 0.000044 0.000965 0.005973 0.000223 0.000063 0.000135 0.004043 0.000831 0.000049 0.000727
Nuclear Periphery 0.000032 0.000060 0.000060 0.000078 0.000102 0.000011 0.000166 0.000050 0.000064 0.000029 0.000091 0.000070
Nuclear Periphery Foci 0.000283 0.000414 0.002531 0.000566 0.000905 0.000076 0.002893 0.000461 0.000568 0.000199 0.002635 0.000536
Nucleolus 0.000392 0.000300 0.000025 0.000079 0.000795 0.000117 0.000080 0.000056 0.000576 0.000184 0.000041 0.000073
Nucleus 0.004898 0.004758 0.006677 0.006741 0.014241 0.001961 0.015460 0.008698 0.009179 0.002984 0.009211 0.007303
Peroxisomes 0.006395 0.013030 0.004891 0.007652 0.014811 0.006016 0.003569 0.006139 0.010251 0.008581 0.004509 0.007218
Vacuole 0.015775 0.029689 0.054853 0.101163 0.020051 0.010996 0.133766 0.180078 0.017734 0.017832 0.077621 0.123821
Vacuole Periphery 0.001660 0.002583 0.000117 0.002252 0.001142 0.000857 0.000186 0.000908 0.001423 0.001488 0.000137 0.001866

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.47 -6.51 0.36 -0.07 6.53 3.68 0.40 3.89 0.88 2.33 3.21 -6.11 2.23 -0.35 6.77
Bud Neck 6.10 16.00 16.74 9.19 0.16 -0.01 11.32 12.96 13.89 -0.04 2.29 20.46 21.96 19.47 0.10
Bud Site 2.63 -2.56 10.19 7.02 9.58 -18.15 -3.56 -0.14 10.10 3.02 -10.95 -5.79 6.51 14.47 9.55
Cell Periphery 7.24 8.28 10.49 6.58 6.04 3.64 8.31 9.38 10.09 3.03 5.49 11.42 13.52 11.23 6.72
Cytoplasm -0.27 2.30 -11.35 -10.38 -10.92 7.69 6.70 -3.34 -7.67 -7.73 5.86 5.36 -11.25 -15.08 -13.21
Cytoplasmic Foci -8.17 0.61 0.23 8.13 -0.41 15.36 8.87 7.36 -5.32 -1.88 5.62 6.87 6.49 1.04 -1.27
Eisosomes 3.90 5.19 6.01 3.33 2.54 5.59 6.40 6.58 3.53 1.56 6.33 7.64 8.07 4.25 2.97
Endoplasmic Reticulum -0.60 -3.19 3.27 2.83 4.08 5.99 -2.39 4.28 -1.95 3.98 4.80 -3.63 5.69 0.76 5.53
Endosome -6.83 5.60 1.26 7.10 -2.58 7.54 7.55 7.21 0.23 -0.43 0.12 9.83 4.95 4.64 -2.58
Golgi -3.91 7.58 0.53 3.38 -2.92 7.23 11.48 11.46 7.04 -0.20 2.52 13.30 4.39 2.60 -2.93
Lipid Particles -1.06 1.83 4.12 4.08 3.71 8.62 7.18 8.35 -2.02 3.32 7.71 7.51 9.23 3.23 4.94
Mitochondria -0.59 7.85 2.91 2.76 -2.62 3.11 9.97 4.86 2.51 -1.01 1.68 12.67 5.52 3.60 -2.77
Mitotic Spindle -2.77 -0.39 -4.42 -3.52 -4.38 -2.84 -1.50 -2.53 0.59 1.14 -3.76 -1.49 -4.71 -2.85 -1.90
None 0.51 5.50 2.23 0.92 -1.48 4.64 4.74 4.71 2.07 -1.14 4.55 6.46 4.67 0.33 -1.51
Nuclear Periphery -1.32 -3.88 -6.27 -2.28 -1.02 5.34 -4.07 1.87 -7.87 4.92 3.06 -4.69 -3.07 -6.28 2.88
Nuclear Periphery Foci -0.99 -3.71 -2.79 -2.61 3.20 1.89 -3.16 0.67 -5.68 4.12 1.70 -4.36 -0.45 -7.13 4.46
Nucleolus 0.25 3.00 2.46 2.32 -2.24 4.03 4.38 4.62 1.23 1.91 3.43 5.40 5.03 2.24 -1.57
Nucleus 0.03 -3.23 -5.41 -5.11 -0.78 8.85 -2.44 0.98 -4.81 2.80 8.20 -2.87 -1.80 -8.52 1.66
Peroxisomes -4.87 1.65 -0.29 3.70 -1.45 5.67 7.96 4.41 0.20 -1.50 1.69 6.52 3.19 1.81 -1.96
Vacuole -5.31 -8.03 -18.03 -15.66 -6.95 5.07 -9.13 -13.45 -14.09 0.03 -0.27 -11.58 -22.26 -22.14 -4.88
Vacuole Periphery -1.57 3.32 -0.29 1.08 -3.18 1.43 8.65 0.61 0.12 -1.02 -0.21 5.00 -0.28 -0.16 -3.32
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication
Localization
Cell Percentages bud neck (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Epo1

Epo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Epo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available