Standard name
Human Ortholog
Description Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0.05 0.1 0.12 0 0.08 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.05 0.07 0.12 0.14 0.12 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.88 0.87 0.88 0.88 0.76 0.8 0.75 0.71 0.66 0.67 0.64 0.87 0.78 0.74 0.86 0.83 0.85 0.78 0.81 0.77 0.74 0.68 0.64
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.31 0.4 0.25 0.24 0.3 0.36 0.41 0.46 0.45 0.53 0.56 0.37 0.42 0.37 0.15 0.11 0.11 0.2 0.13 0.19 0.19 0.21 0.26
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 3 1 1 1 1 0 0 0 0 0 0 0 0 1 0 1 1 1
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 0 5 7 6 10 11 4 10 0 1 2 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 4 4 9 9 18 17 24 7 14 9 10 8 18 24 11 16 19 0 1 0 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 5 0 0 1 0 0 1
Endosome 0 0 0 2 0 0 0 0 0 0 0 0 1 1 1 4 1 3 0 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1
Mitochondria 0 3 5 7 1 17 24 37 42 47 34 46 0 0 4 9 5 8 0 0 0 0 0 1
Nucleus 175 188 222 215 259 233 411 392 239 224 183 208 146 147 154 277 175 294 156 181 206 83 121 143
Nuclear Periphery 0 0 0 0 0 1 2 3 4 6 1 6 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 64 65 101 61 70 91 185 217 154 152 146 181 62 80 77 49 24 39 40 29 49 21 36 57
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 2 3 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 2 0 1 0 0 1 6 4 0 1 0 0 1 0 0 0 1
Vac/Vac Membrane 1 1 1 1 0 0 0 1 0 0 0 0 0 0 0 10 6 14 3 6 5 3 10 10
Unique Cell Count 192 213 255 245 296 305 513 524 338 341 273 323 167 189 207 323 211 347 202 224 267 113 178 223
Labelled Cell Count 241 261 334 296 340 365 651 684 457 456 379 461 219 255 269 364 231 385 202 224 267 113 178 223


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.0 14.8 11.5 12.3 11.6 9.9 9.6 8.7 8.8 8.1 8.0 7.6 17.8 16.5 15.5 14.4 15.9 17.2 16.1 17.3 19.4
Std Deviation (1e-4) 4.8 4.3 3.6 3.1 2.8 2.7 2.6 2.5 2.5 2.6 2.3 2.6 5.7 7.6 8.2 3.9 5.3 8.1 6.2 6.3 6.2
Intensity Change (Log2) – – – 0.09 0.0 -0.21 -0.27 -0.41 -0.38 -0.5 -0.53 -0.61 0.62 0.52 0.43 0.32 0.46 0.58 0.48 0.58 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 1.1 2.6 1.4 2.1 0 1.8 1.3 1.2 0 3.8 4.5 1.4 3.2 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.7 0 2.2 1.9 3.0 4.7 5.1 4.6 5.2 0 0 0 0.6 0 0.3
Nucleus 0.2 0.2 -3.2 -2.4 -3.9 -4.7 -5.9 -5.4 -6.2 0.1 -2.6 -3.5 -0.5 -1.2 -0.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.5 -4.0 -2.4 -1.0 0.5 1.4 1.2 3.2 3.9 -0.5 0.6 -0.5 -6.7 -6.8 -8.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.5361 17.0108 14.2344 15.4513 13.6585 15.1911 13.363 19.0841 16.8433 13.4625 12.2136 15.9169 18.8617 17.6332 16.861 16.0583 17.4065 16.3835
Actin 0.0227 0 0.0038 0 0.006 0.0044 0.007 0 0.0005 0.0049 0.0031 0.0004 0.0115 0 0.0019 0 0 0.0013
Bud 0.0005 0 0.0002 0 0.0002 0 0.0003 0 0 0.0006 0.0003 0 0.0001 0 0 0 0 0
Bud Neck 0.0008 0 0.0004 0.0001 0.0004 0.0004 0.0002 0 0 0.0001 0.0006 0.0003 0 0 0 0 0.0001 0.0002
Bud Periphery 0.0006 0 0.0003 0 0.0004 0.0001 0.0004 0 0 0.0007 0.0004 0 0.0002 0 0 0 0 0
Bud Site 0.013 0 0.0089 0 0.0002 0.0001 0.0012 0 0.0001 0.0008 0.0006 0 0.0001 0 0.0001 0 0.0001 0
Cell Periphery 0.0005 0 0.0003 0 0.0003 0.0001 0.0001 0 0 0.0002 0.0004 0 0.0001 0 0 0 0 0
Cytoplasm 0.0033 0 0.0003 0 0.0002 0 0.0015 0 0.0004 0.0013 0.0026 0.0001 0.0001 0 0 0 0 0.0001
Cytoplasmic Foci 0.0153 0 0.0081 0 0.0095 0.0001 0.0042 0 0.0009 0.0026 0.0071 0.0005 0.0001 0 0.0007 0 0 0.0001
Eisosomes 0.0006 0 0.0001 0 0.0002 0.0001 0.0001 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0.0004
Endoplasmic Reticulum 0.0022 0 0.0006 0 0.0001 0.0003 0.0013 0 0.0001 0.0016 0.0013 0 0.0006 0 0 0 0 0
Endosome 0.0147 0 0.0106 0 0.0013 0.0011 0.0043 0 0.0007 0.0033 0.0019 0.0005 0.0015 0 0.0001 0 0 0.0001
Golgi 0.0046 0 0.0026 0 0.0026 0.0009 0.0015 0 0.0001 0.0003 0.0006 0.0001 0.0029 0 0.0005 0 0 0.0001
Lipid Particles 0.0133 0 0.0168 0 0.0133 0.0004 0.0032 0 0.0004 0.0017 0.0064 0.0003 0.0001 0 0.001 0 0 0.0004
Mitochondria 0.0166 0.0001 0.0045 0.0002 0.0405 0.0007 0.0034 0.0001 0.0005 0.0084 0.0197 0.0002 0.0011 0.0001 0.0011 0.0001 0.0004 0.0004
None 0.0037 0 0.0005 0 0.001 0.0001 0.0016 0 0.0002 0.0013 0.0016 0.0002 0.0001 0 0 0 0 0.011
Nuclear Periphery 0.0169 0.0001 0.0055 0.0001 0.0008 0.0004 0.0142 0.0001 0.0045 0.0451 0.0285 0.0028 0.0003 0.0001 0.0003 0.0005 0.0002 0.0017
Nucleolus 0.1158 0.0525 0.0431 0.0326 0.3464 0.2229 0.0986 0.0488 0.0329 0.0944 0.2466 0.1716 0.0551 0.0355 0.0248 0.018 0.3334 0.1881
Nucleus 0.7355 0.9472 0.8867 0.9667 0.5686 0.7677 0.8073 0.9509 0.9521 0.7923 0.6202 0.8094 0.9254 0.9641 0.9591 0.9812 0.6653 0.7936
Peroxisomes 0.006 0 0.0018 0 0.0015 0 0.0029 0 0.0001 0.0002 0.0005 0.0001 0 0 0.0102 0 0 0.0002
Punctate Nuclear 0.0088 0.0001 0.0011 0 0.0015 0.0001 0.0456 0 0.0059 0.0362 0.041 0.0131 0 0.0001 0.0001 0.0001 0.0003 0.0019
Vacuole 0.0023 0 0.0011 0.0001 0.0015 0.0002 0.0005 0 0.0001 0.0013 0.0023 0.0001 0.0003 0 0 0 0.0001 0.0001
Vacuole Periphery 0.0023 0 0.0028 0.0001 0.0036 0.0001 0.0007 0 0.0003 0.0025 0.0141 0.0001 0.0003 0 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.7628 53.0187 56.6074 75.1534 63.8962 65.6729 59.5849 68.0675 58.5611 76.7648
Translational Efficiency 0.9419 0.9229 0.9965 0.7323 0.8053 0.7738 0.9058 0.6364 0.9135 0.791

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
163 709 89 914 2164 2090 204 219 2327 2799 293 1133

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1209.33 1549.48 2407.61 1609.94 1372.97 1436.75 2031.46 1786.84 1361.51 1465.31 2145.72 1644.13
Standard Deviation 213.82 344.59 391.91 368.16 290.17 299.59 300.47 373.75 288.52 315.44 373.41 375.80
Intensity Change Log 2 — 0.357576 0.993394 0.412799 — 0.065509 0.565217 0.380110 — 0.209669 0.781601 0.395511

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000447 0.000494 0.000922 0.000938 0.000416 0.000539 0.001159 0.000706 0.000418 0.000528 0.001087 0.000893
Bud Neck 0.001742 0.002600 0.005626 0.017038 0.002208 0.005139 0.009421 0.008186 0.002175 0.004496 0.008269 0.015327
Bud Site 0.000206 0.000232 0.000877 0.003258 0.000216 0.000566 0.001117 0.001331 0.000216 0.000481 0.001044 0.002886
Cell Periphery 0.000015 0.000014 0.000019 0.000251 0.000024 0.000078 0.000032 0.000069 0.000023 0.000062 0.000028 0.000216
Cytoplasm 0.000030 0.000052 0.000017 0.001102 0.000110 0.000102 0.000089 0.000268 0.000104 0.000090 0.000067 0.000941
Cytoplasmic Foci 0.000052 0.000058 0.000921 0.004191 0.000028 0.000093 0.000276 0.000259 0.000029 0.000084 0.000472 0.003431
Eisosomes 0.000060 0.000089 0.000256 0.000081 0.000149 0.000126 0.000167 0.000072 0.000143 0.000117 0.000194 0.000079
Endoplasmic Reticulum 0.000504 0.000273 0.000271 0.000750 0.000398 0.000605 0.000611 0.000286 0.000406 0.000521 0.000508 0.000660
Endosome 0.000013 0.000008 0.000007 0.005811 0.000007 0.000102 0.000016 0.000037 0.000007 0.000078 0.000013 0.004695
Golgi 0.000031 0.000039 0.000069 0.002963 0.000023 0.000050 0.000087 0.000067 0.000023 0.000047 0.000082 0.002404
Lipid Particles 0.000004 0.000005 0.000012 0.000348 0.000002 0.000025 0.000019 0.000008 0.000003 0.000020 0.000017 0.000282
Mitochondria 0.000611 0.000876 0.001861 0.004322 0.000628 0.000909 0.002310 0.002059 0.000627 0.000901 0.002173 0.003885
Mitotic Spindle 0.001796 0.000365 0.000036 0.014477 0.000011 0.002615 0.000123 0.004768 0.000136 0.002045 0.000096 0.012600
None 0.000621 0.000561 0.000476 0.001730 0.000572 0.000798 0.000745 0.004379 0.000575 0.000738 0.000663 0.002242
Nuclear Periphery 0.000012 0.000022 0.000021 0.000811 0.000015 0.000071 0.000058 0.000108 0.000014 0.000059 0.000047 0.000675
Nuclear Periphery Foci 0.000006 0.000028 0.000003 0.000680 0.000022 0.000324 0.000010 0.000018 0.000021 0.000249 0.000008 0.000552
Nucleolus 0.075829 0.096823 0.117597 0.049057 0.067447 0.081595 0.061512 0.034106 0.068034 0.085452 0.078548 0.046167
Nucleus 0.911986* 0.892475* 0.836118* 0.880659* 0.925492* 0.903461* 0.910579* 0.940274* 0.924546* 0.900679* 0.887961* 0.892182*
Peroxisomes 0.005784 0.004684 0.034322 0.004173 0.001576 0.002097 0.011067 0.002548 0.001871 0.002752 0.018131 0.003859
Vacuole 0.000102 0.000122 0.000344 0.004001 0.000308 0.000403 0.000423 0.000257 0.000293 0.000332 0.000399 0.003277
Vacuole Periphery 0.000150 0.000181 0.000224 0.003357 0.000347 0.000300 0.000180 0.000196 0.000334 0.000270 0.000193 0.002746

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.59 -7.83 -10.37 -10.09 -2.13 -6.48 -13.80 -6.38 -3.92 5.68 -7.00 -15.86 -12.65 -10.11 1.36
Bud Neck -3.66 -5.66 -12.43 -11.88 -8.62 -11.49 -11.07 -5.40 -2.94 0.96 -11.57 -11.94 -13.02 -10.82 -6.39
Bud Site -0.63 -5.98 -6.52 -6.48 -5.07 -4.52 -10.14 -2.28 -1.51 -0.25 -4.44 -11.49 -6.84 -6.13 -4.63
Cell Periphery 0.14 -0.70 -3.50 -3.53 -3.45 -2.05 -1.82 -2.41 0.39 -1.82 -1.95 -1.47 -3.53 -2.55 -3.41
Cytoplasm -0.77 0.65 -4.10 -4.02 -4.15 -0.08 0.21 -0.97 -0.99 -1.17 0.04 0.46 -3.79 -3.92 -4.07
Cytoplasmic Foci -0.04 -7.43 -3.74 -3.74 -2.75 -2.15 -5.58 -2.69 -1.73 0.40 -2.33 -9.05 -3.82 -3.74 -3.19
Eisosomes -4.19 -10.08 -3.40 1.25 9.15 2.31 -1.66 5.62 4.24 6.48 3.02 -4.58 7.77 6.36 11.63
Endoplasmic Reticulum 4.45 4.38 -3.42 -5.23 -5.20 -4.65 -4.11 2.10 5.41 4.87 -3.33 -2.86 -4.15 -3.00 -2.87
Endosome 0.87 1.24 -3.12 -3.12 -3.12 -1.89 -4.28 -1.67 1.24 -1.07 -1.89 -3.58 -3.12 -3.06 -3.12
Golgi -0.56 -2.89 -2.65 -2.64 -2.60 -2.31 -5.90 -4.11 -2.10 1.11 -2.35 -6.28 -2.67 -2.63 -2.57
Lipid Particles -0.27 -3.59 -2.99 -2.99 -2.92 -1.82 -6.17 -3.34 1.26 3.37 -1.87 -7.05 -3.03 -2.78 -2.82
Mitochondria -4.54 -10.27 -4.52 -4.18 -2.87 -5.17 -12.34 -9.63 -7.62 0.74 -6.27 -14.99 -4.99 -4.54 -2.39
Mitotic Spindle 0.81 0.99 -3.35 -4.56 -4.68 -3.14 -3.72 -1.11 -0.36 -1.08 -3.00 0.27 -4.75 -3.82 -4.77
None 0.66 1.74 -6.29 -6.87 -7.39 -1.24 -1.27 -1.25 -1.15 -1.18 -1.15 -0.90 -3.16 -2.76 -2.95
Nuclear Periphery -2.06 -2.18 -4.11 -4.06 -4.06 -4.05 -6.07 -5.24 -2.27 -2.97 -4.20 -6.17 -4.24 -3.94 -4.01
Nuclear Periphery Foci -1.46 1.39 -3.50 -3.37 -3.51 -1.72 0.83 0.37 1.76 -1.16 -1.73 0.94 -3.38 -1.41 -3.50
Nucleolus -2.38 -2.56 2.34 6.47 4.10 -3.81 0.48 4.62 6.71 2.71 -5.13 -1.60 3.78 8.02 3.53
Nucleus 1.79 3.75 3.10 1.94 -2.01 5.53 2.05 -1.20 -3.59 -2.39 6.59 4.48 5.69 1.98 -0.15
Peroxisomes 0.47 -4.72 0.67 0.73 5.93 -3.00 -5.89 -2.27 -1.19 5.00 -3.04 -8.14 -2.52 -1.38 6.88
Vacuole -1.04 -7.30 -4.12 -4.10 -3.83 -0.48 -1.61 0.09 0.56 2.89 -0.26 -1.59 -3.87 -3.76 -3.71
Vacuole Periphery -0.73 -1.72 -2.18 -2.15 -2.11 0.69 2.78 2.52 2.34 -0.38 1.07 2.53 -1.98 -2.05 -2.13
Standard name
Human Ortholog
Description Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Sto1

Sto1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sto1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available