Standard name | |
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Human Ortholog | |
Description | Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 5 | 7 | 6 | 10 | 11 | 4 | 10 | 0 | 1 | 2 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 4 | 4 | 9 | 9 | 18 | 17 | 24 | 7 | 14 | 9 | 10 | 8 | 18 | 24 | 11 | 16 | 19 | 0 | 1 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 3 | 5 | 7 | 1 | 17 | 24 | 37 | 42 | 47 | 34 | 46 | 0 | 0 | 4 | 9 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 175 | 188 | 222 | 215 | 259 | 233 | 411 | 392 | 239 | 224 | 183 | 208 | 146 | 147 | 154 | 277 | 175 | 294 | 156 | 181 | 206 | 83 | 121 | 143 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 4 | 6 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 64 | 65 | 101 | 61 | 70 | 91 | 185 | 217 | 154 | 152 | 146 | 181 | 62 | 80 | 77 | 49 | 24 | 39 | 40 | 29 | 49 | 21 | 36 | 57 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 6 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 6 | 14 | 3 | 6 | 5 | 3 | 10 | 10 |
Unique Cell Count | 192 | 213 | 255 | 245 | 296 | 305 | 513 | 524 | 338 | 341 | 273 | 323 | 167 | 189 | 207 | 323 | 211 | 347 | 202 | 224 | 267 | 113 | 178 | 223 |
Labelled Cell Count | 241 | 261 | 334 | 296 | 340 | 365 | 651 | 684 | 457 | 456 | 379 | 461 | 219 | 255 | 269 | 364 | 231 | 385 | 202 | 224 | 267 | 113 | 178 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.0 | 14.8 | 11.5 | 12.3 | 11.6 | 9.9 | 9.6 | 8.7 | 8.8 | 8.1 | 8.0 | 7.6 | 17.8 | 16.5 | 15.5 | 14.4 | 15.9 | 17.2 | 16.1 | 17.3 | 19.4 |
Std Deviation (1e-4) | 4.8 | 4.3 | 3.6 | 3.1 | 2.8 | 2.7 | 2.6 | 2.5 | 2.5 | 2.6 | 2.3 | 2.6 | 5.7 | 7.6 | 8.2 | 3.9 | 5.3 | 8.1 | 6.2 | 6.3 | 6.2 |
Intensity Change (Log2) | – | – | – | 0.09 | 0.0 | -0.21 | -0.27 | -0.41 | -0.38 | -0.5 | -0.53 | -0.61 | 0.62 | 0.52 | 0.43 | 0.32 | 0.46 | 0.58 | 0.48 | 0.58 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.5 | 1.1 | 2.6 | 1.4 | 2.1 | 0 | 1.8 | 1.3 | 1.2 | 0 | 3.8 | 4.5 | 1.4 | 3.2 | 2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | 0 | 2.2 | 1.9 | 3.0 | 4.7 | 5.1 | 4.6 | 5.2 | 0 | 0 | 0 | 0.6 | 0 | 0.3 |
Nucleus | 0.2 | 0.2 | -3.2 | -2.4 | -3.9 | -4.7 | -5.9 | -5.4 | -6.2 | 0.1 | -2.6 | -3.5 | -0.5 | -1.2 | -0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -3.5 | -4.0 | -2.4 | -1.0 | 0.5 | 1.4 | 1.2 | 3.2 | 3.9 | -0.5 | 0.6 | -0.5 | -6.7 | -6.8 | -8.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 13.5361 | 17.0108 | 14.2344 | 15.4513 | 13.6585 | 15.1911 | 13.363 | 19.0841 | 16.8433 | 13.4625 | 12.2136 | 15.9169 | 18.8617 | 17.6332 | 16.861 | 16.0583 | 17.4065 | 16.3835 |
Actin | 0.0227 | 0 | 0.0038 | 0 | 0.006 | 0.0044 | 0.007 | 0 | 0.0005 | 0.0049 | 0.0031 | 0.0004 | 0.0115 | 0 | 0.0019 | 0 | 0 | 0.0013 |
Bud | 0.0005 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0.0006 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0008 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0006 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0.0007 | 0.0004 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.013 | 0 | 0.0089 | 0 | 0.0002 | 0.0001 | 0.0012 | 0 | 0.0001 | 0.0008 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0005 | 0 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0033 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0015 | 0 | 0.0004 | 0.0013 | 0.0026 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0153 | 0 | 0.0081 | 0 | 0.0095 | 0.0001 | 0.0042 | 0 | 0.0009 | 0.0026 | 0.0071 | 0.0005 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0.0001 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.0022 | 0 | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0013 | 0 | 0.0001 | 0.0016 | 0.0013 | 0 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0147 | 0 | 0.0106 | 0 | 0.0013 | 0.0011 | 0.0043 | 0 | 0.0007 | 0.0033 | 0.0019 | 0.0005 | 0.0015 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Golgi | 0.0046 | 0 | 0.0026 | 0 | 0.0026 | 0.0009 | 0.0015 | 0 | 0.0001 | 0.0003 | 0.0006 | 0.0001 | 0.0029 | 0 | 0.0005 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0133 | 0 | 0.0168 | 0 | 0.0133 | 0.0004 | 0.0032 | 0 | 0.0004 | 0.0017 | 0.0064 | 0.0003 | 0.0001 | 0 | 0.001 | 0 | 0 | 0.0004 |
Mitochondria | 0.0166 | 0.0001 | 0.0045 | 0.0002 | 0.0405 | 0.0007 | 0.0034 | 0.0001 | 0.0005 | 0.0084 | 0.0197 | 0.0002 | 0.0011 | 0.0001 | 0.0011 | 0.0001 | 0.0004 | 0.0004 |
None | 0.0037 | 0 | 0.0005 | 0 | 0.001 | 0.0001 | 0.0016 | 0 | 0.0002 | 0.0013 | 0.0016 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.011 |
Nuclear Periphery | 0.0169 | 0.0001 | 0.0055 | 0.0001 | 0.0008 | 0.0004 | 0.0142 | 0.0001 | 0.0045 | 0.0451 | 0.0285 | 0.0028 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0017 |
Nucleolus | 0.1158 | 0.0525 | 0.0431 | 0.0326 | 0.3464 | 0.2229 | 0.0986 | 0.0488 | 0.0329 | 0.0944 | 0.2466 | 0.1716 | 0.0551 | 0.0355 | 0.0248 | 0.018 | 0.3334 | 0.1881 |
Nucleus | 0.7355 | 0.9472 | 0.8867 | 0.9667 | 0.5686 | 0.7677 | 0.8073 | 0.9509 | 0.9521 | 0.7923 | 0.6202 | 0.8094 | 0.9254 | 0.9641 | 0.9591 | 0.9812 | 0.6653 | 0.7936 |
Peroxisomes | 0.006 | 0 | 0.0018 | 0 | 0.0015 | 0 | 0.0029 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0 | 0.0102 | 0 | 0 | 0.0002 |
Punctate Nuclear | 0.0088 | 0.0001 | 0.0011 | 0 | 0.0015 | 0.0001 | 0.0456 | 0 | 0.0059 | 0.0362 | 0.041 | 0.0131 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0019 |
Vacuole | 0.0023 | 0 | 0.0011 | 0.0001 | 0.0015 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0013 | 0.0023 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0023 | 0 | 0.0028 | 0.0001 | 0.0036 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0025 | 0.0141 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.7628 | 53.0187 | 56.6074 | 75.1534 | 63.8962 | 65.6729 | 59.5849 | 68.0675 | 58.5611 | 76.7648 |
Translational Efficiency | 0.9419 | 0.9229 | 0.9965 | 0.7323 | 0.8053 | 0.7738 | 0.9058 | 0.6364 | 0.9135 | 0.791 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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