Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function involved in ergosterol biosynthesis; conditional mutants produce less ergosterol, display impaired oxygen consumption, respiratory growth, mitochondrial iron utilization, and are more sensitive to oxidative stress; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; protein localizes to the cytoplasm, ER and nuclear envelope; highly conserved in ascomycetes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 11 | 6 | 3 | 6 | 1 | 3 | – | 0 | 4 | 3 | 0 | 12 | 10 | 9 | 6 | 3 | 14 | 3 | 6 | 0 | 4 | 5 | 5 |
Bud | 0 | 3 | 0 | 0 | 0 | 6 | 1 | – | 3 | 3 | 5 | 7 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 10 | 9 | 4 | 7 | 1 | 10 | – | 7 | 8 | 1 | 17 | 24 | 54 | 61 | 43 | 46 | 57 | 0 | 1 | 0 | 2 | 3 | 2 |
Cytoplasm | 23 | 57 | 24 | 13 | 40 | 69 | 65 | – | 51 | 56 | 45 | 82 | 30 | 32 | 52 | 131 | 120 | 103 | 0 | 11 | 6 | 6 | 9 | 9 |
Endoplasmic Reticulum | 189 | 346 | 163 | 42 | 64 | 35 | 66 | – | 12 | 56 | 16 | 40 | 195 | 267 | 261 | 14 | 13 | 13 | 241 | 404 | 224 | 309 | 311 | 344 |
Endosome | 4 | 1 | 1 | 0 | 1 | 0 | 3 | – | 1 | 2 | 1 | 1 | 0 | 1 | 1 | 11 | 10 | 10 | 2 | 3 | 0 | 2 | 2 | 3 |
Golgi | 1 | 0 | 1 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 17 | 18 | 10 | 3 | 2 | 0 | 0 | 2 | 3 |
Mitochondria | 5 | 4 | 2 | 7 | 8 | 105 | 20 | – | 56 | 50 | 42 | 76 | 3 | 3 | 2 | 13 | 17 | 21 | 6 | 5 | 4 | 5 | 7 | 10 |
Nucleus | 0 | 0 | 3 | 0 | 1 | 4 | 7 | – | 6 | 7 | 4 | 4 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 26 | 6 | 9 | 2 | 1 | 1 | 5 | – | 6 | 2 | 1 | 5 | 5 | 8 | 2 | 3 | 1 | 2 | 7 | 2 | 2 | 0 | 2 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 54 | 54 | 59 | 13 | 80 | 62 | 94 | – | 71 | 64 | 52 | 59 | 17 | 11 | 22 | 213 | 188 | 104 | 6 | 12 | 3 | 9 | 14 | 15 |
Unique Cell Count | 249 | 424 | 220 | 72 | 181 | 230 | 236 | 167 | 207 | 148 | 239 | 245 | 324 | 349 | 400 | 358 | 297 | 279 | 457 | 244 | 347 | 364 | 409 | |
Labelled Cell Count | 311 | 492 | 278 | 84 | 211 | 284 | 274 | 213 | 252 | 171 | 291 | 290 | 388 | 411 | 456 | 418 | 337 | 279 | 457 | 244 | 347 | 364 | 409 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 9.6 | 8.7 | 8.1 | 8.7 | 5.7 | 7.5 | – | 7.1 | 7.2 | 6.7 | 6.7 | 10.9 | 11.3 | 11.5 | 8.8 | 9.2 | 8.6 | 8.7 | 8.6 | 8.8 |
Std Deviation (1e-4) | 1.6 | 1.4 | 1.3 | 2.2 | 1.2 | 1.5 | 1.6 | – | 5.5 | 1.3 | 1.1 | 1.1 | 1.6 | 1.8 | 2.0 | 2.9 | 3.1 | 3.0 | 1.4 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.61 | -0.22 | – | -0.3 | -0.28 | -0.39 | -0.38 | 0.31 | 0.37 | 0.39 | 0.02 | 0.08 | -0.02 | -0.01 | -0.02 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.2 | 0.2 | -0.1 | 0 | 0 | 1.2 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.1 | 0 | 0.1 | – | 0 | -0.1 | 0 | 1.4 | 2.4 | 4.5 | 4.7 | 2.9 | 3.5 | 5.1 |
Cytoplasm | 1.6 | 3.0 | 5.0 | 4.5 | – | 4.8 | 4.3 | 4.7 | 5.9 | 0.4 | -0.4 | 1.4 | 6.0 | 6.1 | 6.2 |
Endoplasmic Reticulum | -2.5 | -7.8 | -12.6 | -9.8 | – | -13.1 | -9.7 | -11.9 | -12.4 | 1.4 | 2.3 | 0.2 | -18.6 | -17.9 | -16.5 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 3.0 | 0 |
Mitochondria | 0 | 0 | 11.1 | 3.8 | – | 8.9 | 7.3 | 8.0 | 8.8 | 0 | 0 | 0 | 1.8 | 2.5 | 3.4 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | -1.2 | – | -0.3 | -2.0 | 0 | -1.2 | -1.3 | -1.1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.5 | 3.6 | 0 | 2.9 | – | 3.2 | 0.9 | 1.7 | -0.5 | -5.8 | -8.0 | -6.8 | 6.3 | 6.1 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3419 | 4.7786 | 4.4906 | 4.5607 | 4.094 | 4.5436 | 5.6646 | 7.0271 | 6.6285 | 6.1665 | 6.6787 | 6.7497 | 10.2499 | 10.2228 | 9.6568 | 10.0541 | 9.5591 | 9.8124 |
Actin | 0.0108 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0053 | 0.0802 | 0.0008 | 0.0048 | 0.0337 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0.0022 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0009 | 0.0034 | 0.0001 | 0.0007 | 0.0046 | 0.0006 | 0.0007 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0.0001 |
Bud Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0015 | 0.0008 | 0.0001 | 0.0014 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Site | 0.0006 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0067 | 0.0022 | 0.0053 | 0.001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0 | 0.001 | 0 | 0 |
Cell Periphery | 0.0003 | 0.0005 | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.0014 | 0.0008 | 0.0003 | 0.0034 | 0.0011 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0.0011 | 0.0002 | 0.0001 |
Cytoplasm | 0.0282 | 0.0513 | 0.0606 | 0.0035 | 0.0049 | 0.0127 | 0.0483 | 0.0533 | 0.0258 | 0.0361 | 0.0249 | 0.0326 | 0.0037 | 0.022 | 0.0042 | 0.0096 | 0.0202 | 0.0117 |
Cytoplasmic Foci | 0.0017 | 0.0008 | 0.0013 | 0.0001 | 0.0002 | 0.0009 | 0.0374 | 0.0022 | 0.0043 | 0.0115 | 0.0007 | 0.0014 | 0.0006 | 0.0014 | 0.0002 | 0.0006 | 0.0003 | 0.0003 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 |
Endoplasmic Reticulum | 0.8737 | 0.9027 | 0.8975 | 0.9293 | 0.9629 | 0.9053 | 0.5224 | 0.8979 | 0.9284 | 0.818 | 0.9253 | 0.9034 | 0.9737 | 0.9505 | 0.9857 | 0.9517 | 0.9604 | 0.9721 |
Endosome | 0.0082 | 0.0075 | 0.0045 | 0.0062 | 0.0029 | 0.0092 | 0.0577 | 0.0074 | 0.0029 | 0.017 | 0.0097 | 0.0121 | 0.0009 | 0.0049 | 0.0013 | 0.0012 | 0.0037 | 0.0022 |
Golgi | 0.0013 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0007 | 0.0209 | 0.0008 | 0.0008 | 0.0125 | 0.0003 | 0.0018 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Lipid Particles | 0.0025 | 0.0014 | 0.0065 | 0.0004 | 0.0004 | 0.0007 | 0.0428 | 0.0084 | 0.0013 | 0.01 | 0.0011 | 0.0008 | 0.0004 | 0.0034 | 0.0015 | 0.0006 | 0.0005 | 0.0005 |
Mitochondria | 0.0058 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.028 | 0.0006 | 0.0002 | 0.0021 | 0.0002 | 0.0033 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 |
None | 0.0021 | 0.0008 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0153 | 0.0013 | 0.0031 | 0.0065 | 0.0001 | 0.0061 | 0.0004 | 0.001 | 0.0001 | 0.0173 | 0.0002 | 0.0007 |
Nuclear Periphery | 0.0537 | 0.0271 | 0.0211 | 0.054 | 0.0236 | 0.0492 | 0.0501 | 0.0156 | 0.0081 | 0.0191 | 0.0194 | 0.025 | 0.0142 | 0.0135 | 0.0049 | 0.0117 | 0.01 | 0.0098 |
Nucleolus | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0111 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0002 | 0 | 0 |
Nucleus | 0.0051 | 0.0015 | 0.0015 | 0.0024 | 0.0016 | 0.0065 | 0.0251 | 0.0016 | 0.002 | 0.0036 | 0.0018 | 0.004 | 0.0008 | 0.0003 | 0.0002 | 0.0006 | 0.001 | 0.0006 |
Peroxisomes | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.016 | 0.0001 | 0.0005 | 0.0061 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0006 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0144 | 0.001 | 0.0097 | 0.0022 | 0.0001 | 0.0017 | 0.0013 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 |
Vacuole | 0.0032 | 0.0036 | 0.0026 | 0.0017 | 0.0017 | 0.0062 | 0.0101 | 0.0026 | 0.0011 | 0.0083 | 0.0131 | 0.0038 | 0.002 | 0.0013 | 0.0011 | 0.0013 | 0.0026 | 0.0012 |
Vacuole Periphery | 0.0011 | 0.0011 | 0.0005 | 0.0012 | 0.0005 | 0.001 | 0.0056 | 0.0004 | 0.0002 | 0.0019 | 0.0011 | 0.0014 | 0.0006 | 0.0002 | 0.0001 | 0.0006 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.8177 | 19.3407 | 12.3014 | 16.2442 | 18.0329 | 15.4453 | 13.9681 | 17.9123 | 21.8989 | 18.2396 |
Translational Efficiency | 2.8543 | 1.4585 | 2.1119 | 1.7862 | 1.2858 | 1.8251 | 2.1706 | 1.6707 | 1.3852 | 1.7799 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2745 | 891 | 2472 | 1606 | 2079 | 2217 | 355 | 1635 | 4824 | 3108 | 2827 | 3241 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 991.22 | 1017.75 | 1337.63 | 1201.59 | 952.76 | 1048.34 | 1231.69 | 1287.49 | 974.64 | 1039.57 | 1324.33 | 1244.92 |
Standard Deviation | 131.41 | 137.53 | 184.67 | 179.65 | 124.92 | 144.46 | 166.28 | 210.14 | 130.06 | 143.18 | 185.81 | 200.28 |
Intensity Change Log 2 | — | 0.038106 | 0.432402 | 0.277668 | — | 0.137922 | 0.370454 | 0.434376 | — | 0.087890 | 0.402373 | 0.356599 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000354 | 0.001074 | 0.000360 | 0.004006 | 0.000449 | 0.000881 | 0.002435 | 0.001494 | 0.000395 | 0.000936 | 0.000620 | 0.002739 |
Bud Neck | 0.000128 | 0.000309 | 0.000735 | 0.008253 | 0.000140 | 0.000791 | 0.002251 | 0.003014 | 0.000133 | 0.000653 | 0.000926 | 0.005610 |
Bud Site | 0.001072 | 0.003331 | 0.002412 | 0.025232 | 0.001233 | 0.003186 | 0.008855 | 0.011060 | 0.001141 | 0.003228 | 0.003221 | 0.018083 |
Cell Periphery | 0.001312 | 0.000748 | 0.000275 | 0.000710 | 0.000611 | 0.000912 | 0.000984 | 0.002643 | 0.001010 | 0.000865 | 0.000364 | 0.001685 |
Cytoplasm | 0.025986 | 0.032751 | 0.041730 | 0.100928* | 0.025662 | 0.048292 | 0.008754 | 0.045170 | 0.025846 | 0.043837 | 0.037589 | 0.072799 |
Cytoplasmic Foci | 0.002761 | 0.009485 | 0.001838 | 0.020349 | 0.004123 | 0.007956 | 0.001276 | 0.012615 | 0.003348 | 0.008395 | 0.001767 | 0.016447 |
Eisosomes | 0.000231 | 0.000112 | 0.000018 | 0.000128 | 0.000073 | 0.000247 | 0.000110 | 0.000102 | 0.000163 | 0.000208 | 0.000030 | 0.000115 |
Endoplasmic Reticulum | 0.940784* | 0.899242* | 0.796807* | 0.488148* | 0.938321* | 0.878853* | 0.924117* | 0.795562* | 0.939722* | 0.884698* | 0.812794* | 0.643230* |
Endosome | 0.001555 | 0.006177 | 0.006632 | 0.024124 | 0.002484 | 0.005082 | 0.006329 | 0.016587 | 0.001955 | 0.005396 | 0.006594 | 0.020322 |
Golgi | 0.003272 | 0.007422 | 0.000393 | 0.003885 | 0.003611 | 0.007515 | 0.000536 | 0.005645 | 0.003418 | 0.007488 | 0.000411 | 0.004773 |
Lipid Particles | 0.004673 | 0.006129 | 0.004183 | 0.023497 | 0.005678 | 0.014352 | 0.001386 | 0.015949 | 0.005106 | 0.011995 | 0.003832 | 0.019689 |
Mitochondria | 0.003532 | 0.005198 | 0.000446 | 0.004443 | 0.001690 | 0.004575 | 0.000156 | 0.001820 | 0.002738 | 0.004753 | 0.000409 | 0.003120 |
Mitotic Spindle | 0.000036 | 0.000605 | 0.000660 | 0.021209 | 0.000070 | 0.000505 | 0.000124 | 0.005349 | 0.000050 | 0.000533 | 0.000593 | 0.013208 |
None | 0.007095 | 0.004033 | 0.001008 | 0.002617 | 0.003733 | 0.005118 | 0.000736 | 0.001909 | 0.005646 | 0.004807 | 0.000974 | 0.002260 |
Nuclear Periphery | 0.001027 | 0.002005 | 0.073961 | 0.058156 | 0.001877 | 0.003647 | 0.005334 | 0.008803 | 0.001393 | 0.003177 | 0.065343 | 0.033259 |
Nuclear Periphery Foci | 0.002526 | 0.011568 | 0.052422 | 0.119844 | 0.006265 | 0.007942 | 0.024584 | 0.031774 | 0.004137 | 0.008982 | 0.048926 | 0.075415 |
Nucleolus | 0.000021 | 0.000041 | 0.000030 | 0.000509 | 0.000092 | 0.000155 | 0.000022 | 0.000122 | 0.000052 | 0.000122 | 0.000029 | 0.000313 |
Nucleus | 0.000719 | 0.001132 | 0.007022 | 0.026595 | 0.001554 | 0.004340 | 0.001074 | 0.008164 | 0.001079 | 0.003420 | 0.006275 | 0.017297 |
Peroxisomes | 0.001960 | 0.001361 | 0.000052 | 0.000883 | 0.000713 | 0.001505 | 0.000095 | 0.000524 | 0.001423 | 0.001463 | 0.000057 | 0.000702 |
Vacuole | 0.000611 | 0.005190 | 0.008786 | 0.063685 | 0.001081 | 0.003126 | 0.009856 | 0.029497 | 0.000814 | 0.003718 | 0.008920 | 0.046438 |
Vacuole Periphery | 0.000348 | 0.002087 | 0.000230 | 0.002799 | 0.000541 | 0.001019 | 0.000986 | 0.002197 | 0.000432 | 0.001325 | 0.000325 | 0.002495 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.04 | -0.41 | -4.17 | -3.15 | -4.11 | -1.93 | -1.72 | -2.62 | -1.68 | 0.64 | -3.13 | -1.44 | -4.93 | -3.72 | -4.21 |
Bud Neck | -2.18 | -5.53 | -13.51 | -13.17 | -12.35 | -2.82 | -1.74 | -7.88 | -5.44 | -0.81 | -3.13 | -4.58 | -15.50 | -13.15 | -12.15 |
Bud Site | -2.77 | -2.52 | -10.24 | -8.96 | -9.36 | -2.79 | -2.48 | -7.21 | -5.47 | -0.86 | -3.85 | -3.45 | -12.45 | -10.47 | -9.93 |
Cell Periphery | 1.85 | 4.09 | 2.27 | 0.10 | -5.22 | -2.01 | -1.01 | -4.68 | -4.03 | -2.96 | 0.84 | 3.94 | -2.61 | -3.59 | -5.91 |
Cytoplasm | -2.46 | -7.19 | -17.78 | -14.75 | -13.25 | -8.66 | 5.71 | -6.52 | -0.03 | -9.55 | -9.19 | -6.28 | -17.84 | -10.48 | -11.76 |
Cytoplasmic Foci | -5.11 | 1.48 | -12.96 | -6.64 | -13.66 | -3.71 | 3.71 | -6.21 | -3.41 | -8.75 | -6.58 | 3.09 | -13.71 | -7.76 | -15.46 |
Eisosomes | 1.51 | 3.25 | 1.45 | -0.40 | -5.03 | -3.89 | -0.44 | -1.25 | 2.99 | 0.09 | -0.92 | 3.35 | 1.10 | 2.48 | -4.50 |
Endoplasmic Reticulum | 5.96 | 24.74 | 50.28* | 39.15* | 31.38* | 10.19 | 1.42 | 17.47 | 9.94 | 10.91 | 12.39 | 24.47 | 44.60 | 33.25 | 22.31 |
Endosome | -8.97 | -13.54 | -19.77 | -14.48 | -14.47 | -5.28 | -3.66 | -12.34 | -9.62 | -6.84 | -9.49 | -12.98 | -22.67 | -17.17 | -15.55 |
Golgi | -3.18 | 7.59 | -0.69 | 2.75 | -6.39 | -4.29 | 6.35 | -1.95 | 1.63 | -5.56 | -5.62 | 10.03 | -2.07 | 3.37 | -8.09 |
Lipid Particles | -1.36 | 0.30 | -11.81 | -10.19 | -11.93 | -5.71 | 5.15 | -6.33 | -1.04 | -9.47 | -6.34 | 1.54 | -12.87 | -5.77 | -13.48 |
Mitochondria | -1.24 | 6.06 | -0.79 | 0.64 | -4.89 | -4.02 | 7.37 | -0.43 | 3.63 | -5.57 | -2.97 | 7.65 | -0.60 | 2.33 | -6.31 |
Mitotic Spindle | -1.50 | -2.41 | -9.96 | -9.60 | -9.58 | -1.48 | -0.97 | -4.48 | -4.02 | -4.43 | -2.05 | -2.39 | -10.82 | -10.32 | -10.17 |
None | 2.16 | 7.50 | 4.36 | 1.09 | -1.77 | -1.38 | 2.92 | 2.16 | 3.69 | -1.39 | 0.92 | 7.68 | 5.07 | 3.40 | -2.29 |
Nuclear Periphery | -2.41 | -23.45 | -16.98 | -16.67 | 4.15 | -3.26 | -3.78 | -6.24 | -4.29 | -2.47 | -4.31 | -23.25 | -17.60 | -16.28 | 10.29 |
Nuclear Periphery Foci | -4.91 | -21.90 | -25.45* | -21.87* | -12.98 | -1.44 | -4.21 | -9.67 | -8.88 | -1.58 | -5.37 | -21.48 | -25.90 | -23.29 | -7.64 |
Nucleolus | -2.00 | -1.39 | -8.11 | -7.75 | -7.95 | -0.92 | 1.10 | -0.59 | 0.67 | -3.93 | -1.34 | 0.81 | -6.61 | -2.42 | -8.76 |
Nucleus | -2.14 | -9.37 | -15.95 | -15.67 | -11.53 | -4.85 | 0.93 | -6.57 | -3.90 | -6.77 | -6.12 | -8.67 | -16.84 | -14.05 | -10.33 |
Peroxisomes | 1.06 | 6.21 | 3.10 | 0.87 | -7.30 | -3.26 | 5.16 | 1.11 | 4.05 | -4.10 | -0.16 | 7.48 | 3.45 | 3.32 | -8.42 |
Vacuole | -4.37 | -11.21 | -21.82 | -19.69 | -18.72 | -4.33 | -3.63 | -12.74 | -11.80 | -6.75 | -6.39 | -11.67 | -24.72 | -22.99 | -19.52 |
Vacuole Periphery | -4.06 | 1.55 | -7.38 | -1.14 | -7.83 | -2.84 | -1.12 | -3.60 | -2.50 | -2.04 | -5.37 | 1.26 | -7.18 | -3.52 | -7.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|