Standard name
Human Ortholog
Description Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.31 0.12 0.39 0.51 0.59 0.55 – 0.47 0.46 0.28 0.35 0.32 0.37 0.48 0.37 0.4 0.43 0.19 0.2 0.2 0.24 0.36 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0.1 0.19 0.08 0.1 0.1 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.06 0.18 0.11 0.05 0.2 0.27 – 0.35 0.32 0.74 0.53 0 0 0 0.07 0 0 0 0 0.07 0 0.05 0.05
Nucleus 0.25 0.44 0.65 0.31 0.14 0.18 0.16 – 0.22 0.25 0.14 0.24 0.39 0.35 0.14 0.15 0.16 0.11 0.32 0.37 0.28 0.34 0.16 0.12
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0.25 0.16 0.11 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.36 0.47 0.35 0.2 0.34 0.14 0.14 – 0.06 0.07 0.07 0.06 0.11 0.11 0.17 0.47 0.46 0.47 0.34 0.34 0.34 0.28 0.3 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2
Bud 0 0 0 0 1 1 3 – 3 2 6 3 0 0 0 0 0 0 0 2 1 1 1 3
Bud Neck 0 0 0 0 2 0 4 – 3 5 2 4 4 1 2 0 0 0 0 0 1 0 1 3
Bud Site 0 0 0 0 0 1 1 – 1 3 12 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 – 1 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 37 24 2 37 102 117 214 – 128 127 102 127 14 54 70 81 119 107 26 21 18 13 75 62
Endoplasmic Reticulum 1 0 0 1 0 0 0 – 0 4 0 3 0 1 0 14 9 20 0 0 0 0 1 1
Endosome 0 0 0 3 11 1 4 – 2 1 2 2 0 14 27 18 29 24 1 1 0 0 2 2
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 4 2 2 0 0 0 0 0 0
Mitochondria 19 5 3 10 10 39 104 – 96 89 266 188 0 5 3 15 4 5 5 1 6 1 9 8
Nucleus 25 34 11 29 27 36 64 – 60 70 49 85 17 51 20 33 48 27 44 39 26 18 33 21
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 1 1 1 0 3 0 0 0 0 0 0 0 0 2 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 5 1 4 – 8 8 6 4 11 24 16 1 3 1 5 2 3 0 6 9
Vac/Vac Membrane 36 36 6 19 67 28 53 – 15 19 26 22 5 16 24 103 135 116 48 36 32 15 62 56
Unique Cell Count 101 77 17 94 200 200 390 271 276 358 358 44 147 145 220 296 246 140 108 95 54 208 182
Labelled Cell Count 119 99 22 102 226 224 451 317 330 473 444 52 169 165 269 350 302 140 108 95 54 208 182


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.7 5.0 5.0 5.7 4.2 4.4 – 4.0 4.0 3.3 3.7 5.9 5.3 5.2 6.2 6.0 5.8 – 4.9 5.2
Std Deviation (1e-4) 1.4 0.9 1.7 1.1 2.2 1.0 1.0 – 1.8 1.2 0.8 0.8 1.4 1.0 1.2 1.4 1.5 1.4 – 1.1 1.7
Intensity Change (Log2) – – – -0.02 0.17 -0.26 -0.2 – -0.33 -0.32 -0.6 -0.44 0.23 0.07 0.04 0.31 0.26 0.2 – -0.04 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.9 0.8 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.0257 -0.9441 -0.7251 -1.2426 -1.1923 -1.0434 -0.1451 -0.1691 -0.3678 0.2457 -0.4098 -0.2503 -1.6262 -1.0871 -1.2231 -1.066 -1.5869 -1.3897
Actin 0.0199 0.0003 0.0029 0.0077 0.0027 0.0009 0.0401 0.0003 0.0058 0.0361 0.0004 0.0044 0.0003 0.0004 0.0055 0.0002 0.0002 0.0003
Bud 0.0005 0.0001 0.0002 0.0001 0.0003 0.0001 0.001 0.0001 0.0002 0.0004 0.0016 0.0002 0.0001 0.0021 0.0001 0 0 0.0001
Bud Neck 0.0013 0.0007 0.0019 0.0018 0.0018 0.0023 0.0078 0.0006 0.0011 0.0022 0.0003 0.0044 0.0004 0.0008 0.0037 0.0005 0.0006 0.0027
Bud Periphery 0.0009 0 0.0005 0.0001 0.0007 0.0001 0.0012 0.0001 0.0003 0.0006 0.0005 0.0002 0.0001 0.0002 0.0001 0 0 0.0001
Bud Site 0.0026 0.0016 0.002 0.0005 0.001 0.0002 0.0135 0.0012 0.0046 0.0019 0.0006 0.0003 0.0003 0.0028 0.0007 0.0001 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0004 0.0002 0.0002 0 0.0005 0.0001 0.0002 0.0002 0 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.1885 0.2413 0.132 0.1907 0.2238 0.1473 0.1607 0.1337 0.1183 0.1746 0.1496 0.1207 0.1012 0.1291 0.0579 0.0907 0.1022 0.0683
Cytoplasmic Foci 0.0267 0.0171 0.0097 0.0138 0.0067 0.0063 0.0325 0.0036 0.0078 0.0147 0.0144 0.0074 0.0053 0.0054 0.0024 0.001 0.0033 0.0051
Eisosomes 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002 0.0001 0.0004 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0091 0.0114 0.0102 0.0095 0.0109 0.0031 0.0057 0.0047 0.003 0.005 0.0027 0.0034 0.0034 0.0072 0.0012 0.0009 0.002 0.0013
Endosome 0.0375 0.0055 0.0181 0.0083 0.0155 0.0112 0.0353 0.0042 0.0068 0.0427 0.0681 0.0073 0.0039 0.0091 0.0031 0.0014 0.0041 0.0036
Golgi 0.0151 0.0002 0.0058 0.0019 0.0054 0.0065 0.0073 0.0001 0.0011 0.0216 0.0031 0.0023 0.0002 0.0005 0.0001 0 0.0001 0.0001
Lipid Particles 0.0127 0.0039 0.0145 0.0066 0.0846 0.0161 0.0118 0.0004 0.0015 0.0567 0.0006 0.0082 0.0013 0.0008 0.0005 0.0001 0.0001 0.0005
Mitochondria 0.0186 0.0005 0.0102 0.0009 0.0034 0.0021 0.0061 0.001 0.0014 0.0192 0.0021 0.0014 0.0025 0.0013 0.0009 0.0008 0.0006 0.0006
None 0.234 0.269 0.3691 0.1626 0.3657 0.2328 0.2568 0.3941 0.334 0.4396 0.3398 0.1141 0.2503 0.222 0.2396 0.2506 0.2244 0.1398
Nuclear Periphery 0.0388 0.0198 0.0419 0.0594 0.0376 0.0142 0.0269 0.0205 0.0279 0.015 0.0121 0.0226 0.0241 0.0261 0.0228 0.0151 0.0174 0.0183
Nucleolus 0.0035 0.0041 0.0152 0.0021 0.0058 0.0049 0.0144 0.0061 0.0183 0.0024 0.005 0.0112 0.0102 0.0079 0.0098 0.0054 0.0077 0.0117
Nucleus 0.3393 0.397 0.3278 0.5001 0.2007 0.5151 0.3105 0.3977 0.3993 0.1233 0.3631 0.649 0.5377 0.5384 0.5918 0.61 0.5905 0.6795
Peroxisomes 0.0137 0.0003 0.0131 0.0009 0.0013 0.0016 0.0046 0.0001 0.0004 0.0106 0.0002 0.003 0.0031 0.0002 0.0003 0.0001 0.0003 0.0002
Punctate Nuclear 0.0256 0.0225 0.0154 0.0293 0.0277 0.0338 0.0509 0.0276 0.0631 0.0218 0.0306 0.0369 0.0526 0.0425 0.0576 0.022 0.0443 0.0664
Vacuole 0.0084 0.0041 0.0071 0.0023 0.0027 0.0012 0.0089 0.0031 0.0043 0.0083 0.0037 0.0021 0.0025 0.0024 0.0014 0.0007 0.0015 0.001
Vacuole Periphery 0.0026 0.0004 0.0019 0.0009 0.0015 0.0003 0.0032 0.0006 0.0007 0.0028 0.0015 0.0007 0.0006 0.0006 0.0004 0.0003 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.0558 3.9733 2.5798 4.3176 1.4407 3.3317 3.5152 5.423 4.5115 5.4645
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1995 703 1730 1276 1709 2151 1798 2305 3704 2854 3528 3581

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 637.76 649.09 892.58 841.46 688.25 682.39 881.30 771.49 661.06 674.19 886.83 796.42
Standard Deviation 69.16 111.21 106.28 121.79 77.10 89.21 103.49 96.91 77.15 96.18 105.02 111.59
Intensity Change Log 2 — 0.025405 0.484968 0.399881 — -0.012336 0.356701 0.164715 — 0.005939 0.419817 0.282610

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000071 0.000758 0.000287 0.001236 0.000070 0.000163 0.000106 0.000240 0.000071 0.000310 0.000195 0.000595
Bud Neck 0.009673 0.016317 0.008444 0.020858 0.004356 0.013512 0.005995 0.008771 0.007220 0.014203 0.007196 0.013078
Bud Site 0.003554 0.006097 0.002652 0.030801 0.000940 0.006722 0.001648 0.005795 0.002348 0.006568 0.002140 0.014706
Cell Periphery 0.000172 0.000188 0.000072 0.000219 0.000106 0.000366 0.000061 0.000278 0.000142 0.000322 0.000066 0.000257
Cytoplasm 0.409418* 0.227601* 0.028316 0.118645* 0.292065* 0.216092* 0.076973 0.133809* 0.355273* 0.218927* 0.053114 0.128406*
Cytoplasmic Foci 0.073622 0.110205 0.000422 0.003146 0.067508 0.048707 0.000474 0.003695 0.070801 0.063855 0.000449 0.003500
Eisosomes 0.000072 0.000106 0.000016 0.000039 0.000050 0.000070 0.000010 0.000037 0.000062 0.000079 0.000013 0.000038
Endoplasmic Reticulum 0.000551 0.002916 0.004936 0.008574 0.000758 0.001480 0.002564 0.001452 0.000647 0.001834 0.003727 0.003990
Endosome 0.002842 0.010847 0.000494 0.004481 0.001960 0.005020 0.000291 0.001708 0.002435 0.006455 0.000390 0.002696
Golgi 0.001354 0.002413 0.000033 0.002048 0.000633 0.002073 0.000019 0.000211 0.001021 0.002157 0.000026 0.000866
Lipid Particles 0.005753 0.014237 0.000280 0.001236 0.004963 0.007451 0.000180 0.000568 0.005388 0.009122 0.000229 0.000806
Mitochondria 0.004698 0.017669 0.001148 0.004803 0.002557 0.011922 0.000663 0.001899 0.003710 0.013338 0.000901 0.002934
Mitotic Spindle 0.003112 0.003689 0.001956 0.041153 0.003515 0.007703 0.002189 0.007510 0.003298 0.006714 0.002075 0.019498
None 0.011505 0.027336 0.002644 0.006565 0.006156 0.009642 0.003632 0.008334 0.009037 0.014001 0.003147 0.007703
Nuclear Periphery 0.000893 0.001613 0.001566 0.003077 0.001082 0.002295 0.001510 0.001638 0.000980 0.002127 0.001537 0.002151
Nuclear Periphery Foci 0.000337 0.002059 0.000211 0.001367 0.000433 0.000486 0.000157 0.000279 0.000381 0.000873 0.000183 0.000666
Nucleolus 0.003805 0.013007 0.000770 0.001330 0.002060 0.007182 0.000380 0.001739 0.003000 0.008617 0.000571 0.001593
Nucleus 0.455415* 0.493959* 0.939250* 0.709083* 0.599080* 0.634316* 0.896780* 0.812308* 0.521701* 0.599743* 0.917606* 0.775526*
Peroxisomes 0.002704 0.022684 0.000116 0.000399 0.002097 0.005785 0.000046 0.000469 0.002424 0.009948 0.000080 0.000444
Vacuole 0.010030 0.024556 0.006202 0.039344 0.009319 0.017421 0.006275 0.008558 0.009702 0.019179 0.006239 0.019528
Vacuole Periphery 0.000420 0.001742 0.000186 0.001596 0.000291 0.001593 0.000047 0.000703 0.000360 0.001630 0.000115 0.001021

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.64 -6.36 -8.38 -1.81 -5.54 -3.59 -4.11 -1.89 -0.90 -1.04 -4.80 -7.44 -6.48 -2.91 -3.76
Bud Neck -2.58 -0.10 -6.88 -2.55 -7.03 -7.19 -2.35 -4.74 3.12 -2.24 -5.92 -1.21 -6.87 -0.02 -5.94
Bud Site -1.64 0.76 -7.08 -5.48 -7.43 -6.07 -1.94 -5.07 1.02 -3.80 -4.89 -0.01 -7.95 -3.35 -8.01
Cell Periphery -1.11 7.70 -1.42 -0.29 -9.75 -2.65 8.52 -2.86 1.22 -3.91 -2.45 10.28 -2.65 1.28 -5.29
Cytoplasm 17.34 65.26* 43.14 12.48 -15.81 10.10 33.93 23.91 14.54 -11.59 23.01 67.92 46.99 19.53 -20.22
Cytoplasmic Foci -5.80 28.03 26.96 18.37 -6.40 4.78 22.47 21.33 20.25 -8.76 2.01 35.74 34.09 26.76 -10.74
Eisosomes -6.18 17.70 1.38 7.03 -15.47 -2.91 15.99 2.87 4.38 -5.04 -3.43 23.76 4.18 5.69 -9.77
Endoplasmic Reticulum -7.97 -21.30 -22.04 -15.22 -10.36 -7.29 -12.84 -12.93 -5.54 5.97 -11.38 -24.28 -23.64 -16.63 -3.22
Endosome -6.16 7.77 -0.98 5.63 -6.64 -6.11 9.59 1.24 6.37 -4.70 -7.95 11.33 0.82 8.08 -7.72
Golgi -1.96 3.64 1.02 2.98 -2.23 -3.09 3.22 2.17 4.38 -1.82 -2.85 4.65 2.39 4.82 -2.76
Lipid Particles -5.71 9.22 6.85 8.88 -9.74 -3.55 10.17 9.31 12.52 -7.21 -5.88 13.39 11.46 14.98 -11.66
Mitochondria -4.44 3.99 1.24 5.01 -3.70 -6.39 2.82 0.99 7.30 -2.80 -7.22 4.92 2.09 8.70 -4.32
Mitotic Spindle -0.76 0.88 -8.59 -8.31 -8.86 -2.68 0.38 -3.14 -0.54 -3.61 -3.15 0.80 -8.81 -6.13 -9.31
None -4.01 8.25 4.36 5.53 -6.63 -3.32 4.19 -1.53 1.67 -5.22 -3.71 9.24 2.12 5.28 -7.48
Nuclear Periphery -3.80 -8.44 -15.55 -12.35 -12.55 -6.10 -10.24 -6.29 -0.01 -1.05 -7.14 -12.73 -15.04 -6.78 -9.19
Nuclear Periphery Foci -4.49 0.48 -6.98 -2.77 -7.04 -1.27 2.45 0.57 1.66 -1.49 -4.45 2.07 -6.44 -2.92 -7.04
Nucleolus -4.90 6.16 -0.84 4.35 -5.68 -7.11 6.91 -0.44 6.52 -5.48 -7.47 8.36 -0.03 7.33 -7.68
Nucleus -2.38 -70.22 -21.90 -13.08 27.18 -3.40 -36.81 -24.52 -21.64 13.14 -9.79 -73.00 -37.13 -22.70 28.38
Peroxisomes -6.65 8.28 7.18 7.50 -4.04 -5.77 7.48 5.82 9.27 -2.96 -8.50 11.14 9.10 11.21 -4.02
Vacuole -7.47 1.02 -16.60 -11.65 -16.71 -6.00 -2.11 -4.79 0.97 -2.10 -8.75 -1.05 -16.09 -9.41 -14.82
Vacuole Periphery -4.87 3.62 -1.57 3.06 -2.83 -4.78 5.99 -1.70 3.15 -3.21 -5.86 6.34 -2.09 3.96 -4.06
Standard name
Human Ortholog
Description Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (21%), cytoplasm (8%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pso2

Pso2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pso2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available