Standard name
Human Ortholog
Description Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.16 0.12 0 0 0 0 0 0
Cytoplasm 0.92 0.95 0.95 0.97 0.96 0.91 0.86 0.88 0.81 0.79 0.74 0.77 0.97 0.99 0.99 0.93 0.9 0.95 0.88 0.93 0.86 0.9 0.97 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.19 0.1 0.24 0.16 0.34 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.08 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 0 0 0 0 1 2 1 1 0 2 2 0 0 0 1 1 1 1 1 0 0 3
Bud 5 1 2 3 0 4 7 5 12 12 10 8 1 1 0 0 0 0 0 0 1 1 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 6 6 7 9 10 16 15 13 13 11 18 5 7 10 16 24 14 0 0 0 0 0 1
Cytoplasm 294 250 261 337 515 497 517 466 431 438 358 384 206 417 341 122 131 109 286 241 245 184 426 362
Endoplasmic Reticulum 9 10 4 1 0 4 4 6 2 1 5 15 4 3 4 3 3 6 10 5 11 3 5 7
Endosome 4 0 3 3 2 1 1 0 1 0 0 0 1 0 0 0 3 0 8 2 5 2 1 2
Golgi 3 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 1 0 3 3 0 0 0 0
Mitochondria 13 4 0 5 7 64 115 54 128 91 166 82 0 0 0 0 1 0 3 0 5 0 0 2
Nucleus 0 1 1 1 4 1 1 1 1 9 2 2 0 0 1 2 3 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2 2 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 1 0 1 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0
SpindlePole 0 0 3 0 0 1 0 1 1 0 0 1 1 0 0 0 1 0 1 0 1 0 0 1
Vac/Vac Membrane 0 1 4 4 21 18 25 18 15 45 14 42 0 0 1 2 4 0 2 0 2 4 0 2
Unique Cell Count 319 263 275 348 537 549 599 531 532 556 485 500 213 421 345 131 146 115 325 260 286 204 440 394
Labelled Cell Count 332 274 285 362 559 601 687 570 608 611 566 555 220 429 357 146 172 130 325 260 286 204 440 394


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 9.4 7.9 7.7 8.0 6.6 6.4 7.0 6.0 6.4 5.8 6.5 8.5 9.3 9.4 14.3 14.3 14.0 7.7 8.5 9.4
Std Deviation (1e-4) 1.0 1.1 1.1 1.3 1.6 1.4 1.4 1.6 1.2 1.2 1.2 1.2 1.0 1.2 1.3 3.0 2.8 2.5 1.3 1.6 1.7
Intensity Change (Log2) -0.04 0.02 -0.25 -0.31 -0.17 -0.39 -0.29 -0.45 -0.29 0.11 0.24 0.25 0.85 0.86 0.82 -0.03 0.11 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -0.5 -0.4 0.4 0.5 0.2 0.1 0.1 1.1 0 -0.5 0.6 4.2 5.4 4.1
Cytoplasm 1.2 0.7 -2.2 -3.8 -3.2 -5.4 -6.0 -7.2 -6.5 1.0 3.4 2.9 -0.7 -2.0 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 7.8 5.5 8.9 7.1 11.0 7.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.9 1.5 2.1 1.6 1.2 3.8 1.2 3.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9247 6.3067 5.8197 5.3179 5.0372 5.8238 4.6825 5.5129 5.0124 4.5125 4.1773 5.2283 4.496 5.547 4.8286 4.7066 4.1145 5.091
Actin 0.0205 0.002 0.0103 0.0004 0.0001 0.0037 0.0123 0.0002 0.0102 0.0011 0.0005 0.0024 0.0429 0.0006 0.0299 0.0002 0.0316 0.0104
Bud 0.0047 0.0068 0.0073 0.0003 0.0001 0.0007 0.0049 0.0002 0.0042 0.0043 0.0009 0.0001 0.0035 0.0005 0.0099 0.0006 0.0009 0.0041
Bud Neck 0.0038 0.0003 0.0006 0.0005 0.0004 0.0047 0.0033 0.0003 0.0037 0.0005 0.0013 0.0012 0.0033 0.0003 0.0056 0.0003 0.0009 0.0016
Bud Periphery 0.003 0.0031 0.0035 0.0001 0 0.0005 0.0026 0.0001 0.0025 0.0015 0.001 0.0002 0.002 0.0001 0.0045 0.0002 0.0009 0.0021
Bud Site 0.0073 0.0188 0.003 0.0003 0 0.0003 0.0082 0.0011 0.0172 0.0013 0.0141 0.0002 0.0056 0.014 0.0166 0.0003 0.0013 0.0008
Cell Periphery 0.0007 0.0006 0.0003 0.0001 0 0.0001 0.0005 0.0001 0.0003 0.0004 0.0001 0.0001 0.0006 0.0004 0.0004 0.0001 0.0001 0.0003
Cytoplasm 0.6047 0.8249 0.8734 0.8695 0.9748 0.7004 0.6486 0.9023 0.7962 0.8568 0.7921 0.7194 0.6169 0.8888 0.8015 0.9502 0.7977 0.6405
Cytoplasmic Foci 0.1082 0.0597 0.0247 0.0455 0.007 0.1056 0.0911 0.0308 0.0482 0.0267 0.048 0.1152 0.0781 0.0325 0.028 0.0113 0.0482 0.116
Eisosomes 0.0003 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0004 0 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0269 0.0105 0.004 0.0046 0.0009 0.0182 0.0177 0.004 0.0045 0.004 0.017 0.0114 0.0422 0.0081 0.0265 0.0034 0.0035 0.0257
Endosome 0.1438 0.0493 0.0337 0.0634 0.007 0.1257 0.1344 0.0407 0.0512 0.066 0.0712 0.1101 0.108 0.038 0.0458 0.0154 0.0547 0.1501
Golgi 0.0076 0.0016 0.0034 0.0014 0.0002 0.0074 0.0102 0.0015 0.0038 0.0071 0.0053 0.007 0.0139 0.0036 0.0064 0.0003 0.02 0.0107
Lipid Particles 0.0186 0.0022 0.0037 0.0011 0 0.0157 0.0111 0.0002 0.0017 0.0034 0.0047 0.0085 0.011 0.0031 0.0021 0.0002 0.0062 0.0083
Mitochondria 0.0054 0.0012 0.0013 0.0002 0.0001 0.0012 0.0091 0.0003 0.0078 0.0036 0.0077 0.0015 0.0046 0.0002 0.0022 0.0001 0.0065 0.0017
None 0.0137 0.0091 0.0115 0.0052 0.0063 0.0061 0.0169 0.013 0.0226 0.0105 0.0136 0.0074 0.0278 0.005 0.0042 0.0054 0.0115 0.004
Nuclear Periphery 0.0036 0.0009 0.0008 0.0011 0.0002 0.0018 0.0036 0.0005 0.0045 0.0012 0.0018 0.0024 0.0106 0.0006 0.0057 0.0015 0.0018 0.0021
Nucleolus 0.0005 0.0007 0.0003 0 0 0.0001 0.0009 0 0.0005 0.0002 0.0011 0.0002 0.0008 0 0.0002 0 0.0001 0.0003
Nucleus 0.002 0.0016 0.0012 0.0008 0.0004 0.0008 0.0035 0.0012 0.0038 0.0019 0.0028 0.0014 0.0038 0.0007 0.0025 0.0015 0.0012 0.0043
Peroxisomes 0.0081 0.0005 0.01 0.0003 0 0.0011 0.006 0.0001 0.0056 0.0006 0.0047 0.0019 0.007 0.0002 0.0013 0.0001 0.0054 0.005
Punctate Nuclear 0.0046 0.0012 0.0038 0.0004 0 0.0013 0.0027 0.0003 0.0039 0.0009 0.0045 0.0047 0.0071 0.0002 0.0013 0.0008 0.0027 0.0023
Vacuole 0.0108 0.0042 0.0027 0.004 0.0022 0.0038 0.0109 0.0027 0.0065 0.0067 0.0062 0.0041 0.0084 0.0028 0.0042 0.0072 0.0033 0.0082
Vacuole Periphery 0.0013 0.0007 0.0005 0.0008 0.0003 0.0009 0.0015 0.0004 0.0012 0.0013 0.0014 0.0008 0.0015 0.0003 0.001 0.0009 0.0013 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.8448 11.521 26.9625 23.2019 10.9256 12.8808 19.4895 23.5866 22.2564 23.0328
Translational Efficiency 0.8254 1.3048 0.5442 0.8321 1.33 1.6316 0.9968 0.7649 1.0208 0.7196

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Rim11

Rim11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available