ORF | |
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Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 2 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 1 |
Bud | 1 | 1 | 0 | 0 | 0 | 3 | 2 | – | 1 | 9 | 6 | 4 | 2 | 0 | 1 | 0 | 1 | 0 | 4 | 5 | 2 | 3 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 4 | 1 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 106 | 151 | 58 | 43 | 78 | 129 | 182 | – | 97 | 114 | 112 | 111 | 118 | 175 | 211 | 97 | 107 | 97 | 8 | 21 | 14 | 13 | 12 | 13 |
Endoplasmic Reticulum | 10 | 15 | 10 | 0 | 0 | 5 | 2 | – | 0 | 0 | 2 | 2 | 4 | 0 | 7 | 10 | 14 | 14 | 3 | 2 | 1 | 3 | 7 | 6 |
Endosome | 26 | 9 | 0 | 0 | 3 | 3 | 2 | – | 1 | 0 | 0 | 0 | 6 | 16 | 24 | 10 | 15 | 31 | 46 | 24 | 7 | 33 | 42 | 45 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 1 | 1 | 2 |
Mitochondria | 42 | 22 | 2 | 4 | 1 | 14 | 28 | – | 48 | 39 | 61 | 48 | 28 | 12 | 15 | 17 | 21 | 16 | 16 | 6 | 18 | 7 | 7 | 9 |
Nucleus | 25 | 7 | 4 | 1 | 0 | 1 | 1 | – | 5 | 6 | 1 | 1 | 3 | 2 | 8 | 21 | 35 | 14 | 5 | 4 | 1 | 0 | 1 | 1 |
Nuclear Periphery | 6 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 2 | 3 | 0 | 4 | 7 | 1 | 21 | 3 | 3 | 8 | 12 | 9 |
Nucleolus | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
SpindlePole | 2 | 1 | 0 | 0 | 1 | 1 | 4 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 3 | 2 | 0 | 2 | 2 |
Vac/Vac Membrane | 220 | 103 | 6 | 0 | 16 | 12 | 9 | – | 6 | 7 | 5 | 4 | 155 | 175 | 167 | 201 | 260 | 215 | 317 | 236 | 87 | 246 | 266 | 236 |
Unique Cell Count | 374 | 270 | 79 | 46 | 96 | 156 | 205 | 128 | 143 | 139 | 136 | 279 | 331 | 384 | 303 | 388 | 315 | 440 | 316 | 144 | 327 | 365 | 343 | |
Labelled Cell Count | 442 | 313 | 84 | 52 | 101 | 171 | 233 | 158 | 177 | 187 | 174 | 318 | 383 | 434 | 361 | 462 | 394 | 440 | 316 | 144 | 327 | 365 | 343 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 5.1 | 4.7 | 4.5 | 5.4 | 4.2 | 4.6 | – | 4.0 | 4.1 | 3.8 | 4.0 | 4.5 | 4.7 | 4.8 | 6.6 | 6.8 | 7.1 | 4.3 | 4.6 | 5.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.7 | 0.8 | 1.2 | 0.9 | 1.4 | – | 1.3 | 1.7 | 1.1 | 0.9 | 0.9 | 1.1 | 1.3 | 1.5 | 1.3 | 1.5 | 1.1 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.2 | -0.18 | -0.05 | – | -0.25 | -0.21 | -0.34 | -0.23 | -0.07 | -0.02 | 0.01 | 0.48 | 0.52 | 0.58 | -0.13 | -0.05 | 0.08 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 1.2 | 1.7 | 3.2 | – | 0.4 | 1.1 | 1.2 | 1.4 | -4.9 | -3.3 | -3.0 | -6.7 | -7.8 | -6.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 1.2 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 7.6 | 7.3 | 6.0 | 9.3 | 9.7 | 9.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3307 | 2.8921 | 2.4626 | 2.2634 | 2.4876 | 2.5691 | 0.2875 | 0.3715 | 0.2557 | 0.0546 | 0.6387 | 0.252 | 0.1157 | 0.3389 | 0.047 | 0.326 | 0.5113 | 0.3648 |
Actin | 0.0409 | 0.0004 | 0.0105 | 0.0024 | 0.0005 | 0.0016 | 0.0201 | 0.0001 | 0.0104 | 0.0017 | 0.0083 | 0.0011 | 0.0223 | 0.004 | 0.0062 | 0.0001 | 0.0149 | 0.0083 |
Bud | 0.0016 | 0.0023 | 0.0013 | 0.0029 | 0.0007 | 0.0007 | 0.0032 | 0.0049 | 0.0013 | 0.005 | 0.0102 | 0.002 | 0.0024 | 0.0014 | 0.0057 | 0.0003 | 0.0004 | 0.0003 |
Bud Neck | 0.0126 | 0.0018 | 0.0035 | 0.0038 | 0.0006 | 0.0031 | 0.002 | 0.0007 | 0.0055 | 0.001 | 0.0018 | 0.0017 | 0.0019 | 0.0028 | 0.0008 | 0.0002 | 0.0005 | 0.001 |
Bud Periphery | 0.0026 | 0.003 | 0.0027 | 0.0057 | 0.0023 | 0.0019 | 0.0037 | 0.001 | 0.0016 | 0.0141 | 0.0227 | 0.0011 | 0.0032 | 0.0056 | 0.0021 | 0.0003 | 0.0005 | 0.0004 |
Bud Site | 0.013 | 0.0121 | 0.0027 | 0.0064 | 0.0009 | 0.0008 | 0.007 | 0.0185 | 0.006 | 0.0025 | 0.0088 | 0.0013 | 0.0079 | 0.0017 | 0.0055 | 0.0002 | 0.0008 | 0.0003 |
Cell Periphery | 0.0012 | 0.0009 | 0.0007 | 0.0007 | 0.001 | 0.0009 | 0.0008 | 0.0007 | 0.0009 | 0.0006 | 0.001 | 0.0002 | 0.0006 | 0.0007 | 0.0005 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0747 | 0.1459 | 0.1189 | 0.0502 | 0.101 | 0.1572 | 0.1153 | 0.263 | 0.1651 | 0.0517 | 0.1288 | 0.1233 | 0.0954 | 0.1501 | 0.1382 | 0.1024 | 0.1401 | 0.1373 |
Cytoplasmic Foci | 0.0561 | 0.0327 | 0.0503 | 0.0566 | 0.0862 | 0.0528 | 0.0585 | 0.0379 | 0.0584 | 0.0329 | 0.0794 | 0.0542 | 0.0522 | 0.0261 | 0.0444 | 0.0369 | 0.0331 | 0.0583 |
Eisosomes | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0004 | 0 |
Endoplasmic Reticulum | 0.0081 | 0.007 | 0.0077 | 0.0048 | 0.004 | 0.0063 | 0.0095 | 0.0049 | 0.0057 | 0.0058 | 0.003 | 0.0042 | 0.0089 | 0.006 | 0.0057 | 0.002 | 0.0048 | 0.0055 |
Endosome | 0.2177 | 0.1767 | 0.242 | 0.2345 | 0.2637 | 0.2683 | 0.3061 | 0.2613 | 0.289 | 0.367 | 0.3914 | 0.3495 | 0.2438 | 0.2075 | 0.2737 | 0.3249 | 0.2895 | 0.3376 |
Golgi | 0.0124 | 0.0031 | 0.0086 | 0.0063 | 0.0066 | 0.0048 | 0.0186 | 0.0023 | 0.0083 | 0.0113 | 0.0393 | 0.0049 | 0.0134 | 0.0013 | 0.0036 | 0.0015 | 0.0241 | 0.0044 |
Lipid Particles | 0.0234 | 0.0039 | 0.0094 | 0.0111 | 0.0095 | 0.0058 | 0.0252 | 0.002 | 0.0088 | 0.0049 | 0.0119 | 0.0034 | 0.0213 | 0.0018 | 0.0045 | 0.002 | 0.0254 | 0.0097 |
Mitochondria | 0.0192 | 0.0094 | 0.0166 | 0.0172 | 0.0392 | 0.0126 | 0.0291 | 0.0016 | 0.013 | 0.0082 | 0.0142 | 0.0055 | 0.0117 | 0.0017 | 0.0073 | 0.0018 | 0.012 | 0.0035 |
None | 0.2242 | 0.3349 | 0.2714 | 0.142 | 0.1212 | 0.199 | 0.0684 | 0.1072 | 0.1085 | 0.058 | 0.0179 | 0.0593 | 0.1505 | 0.1287 | 0.1054 | 0.0886 | 0.1182 | 0.1172 |
Nuclear Periphery | 0.0121 | 0.0068 | 0.0157 | 0.007 | 0.0335 | 0.0072 | 0.0202 | 0.0043 | 0.0069 | 0.0101 | 0.0034 | 0.0134 | 0.0125 | 0.0074 | 0.014 | 0.0039 | 0.0088 | 0.0068 |
Nucleolus | 0.0026 | 0.0013 | 0.0024 | 0.0039 | 0.0011 | 0.0017 | 0.0048 | 0.0016 | 0.0023 | 0.0023 | 0.0006 | 0.0016 | 0.003 | 0.0011 | 0.0021 | 0.0005 | 0.0004 | 0.0011 |
Nucleus | 0.0064 | 0.0043 | 0.0096 | 0.0104 | 0.0055 | 0.0108 | 0.0162 | 0.0106 | 0.0075 | 0.0129 | 0.0028 | 0.0091 | 0.011 | 0.0049 | 0.017 | 0.0023 | 0.0027 | 0.0082 |
Peroxisomes | 0.0121 | 0.0005 | 0.0073 | 0.0064 | 0.0162 | 0.0021 | 0.013 | 0.0003 | 0.0044 | 0.0013 | 0.0033 | 0.0008 | 0.0115 | 0.0003 | 0.0029 | 0.0005 | 0.0086 | 0.0023 |
Punctate Nuclear | 0.0037 | 0.001 | 0.002 | 0.0158 | 0.001 | 0.0013 | 0.0036 | 0.0016 | 0.0117 | 0.0027 | 0.0009 | 0.0031 | 0.0036 | 0.0009 | 0.0034 | 0.0003 | 0.0013 | 0.0061 |
Vacuole | 0.2279 | 0.2085 | 0.1789 | 0.3431 | 0.2381 | 0.2102 | 0.2367 | 0.2288 | 0.225 | 0.3135 | 0.1917 | 0.2925 | 0.2852 | 0.3791 | 0.2932 | 0.3581 | 0.2306 | 0.2473 |
Vacuole Periphery | 0.0268 | 0.0433 | 0.0375 | 0.0687 | 0.0671 | 0.0507 | 0.0376 | 0.0468 | 0.0595 | 0.0926 | 0.0586 | 0.0676 | 0.0374 | 0.0668 | 0.0638 | 0.0732 | 0.0827 | 0.0441 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 4.2768 | 3.2195 | 3.2849 | 7.8261 | 6.7124 | 4.0494 | 8.0364 | 9.4749 | 7.9563 | 8.9274 |
Translational Efficiency | 1.0199 | 1.0388 | 1.0082 | 0.5338 | 0.5351 | 1.1513 | 0.5639 | 0.6316 | 0.6196 | 0.6175 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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