Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.23 0.05 0 0 0 0 0 – 0 0 0 0.09 0.07 0 0 0.06 0 0.16 0.12 0.17 0.21 0.19 0.17
Bud 0 0.06 0.1 0 0 0.07 0.07 0.08 – 0.06 0.11 0.11 0 0 0 0 0 0.06 0 0.06 0 0.14 0.1 0.07
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0.05
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.44 0.44 0.68 0.73 0.68 0.63 0.59 – 0.57 0.58 0.5 0.7 0.6 0.59 0.85 0.79 0.83 0.08 0.09 0.05 0.07 0.09 0.11
Endoplasmic Reticulum 0.15 0.19 0 0 0 0 0 0 – 0 0 0 0.09 0.16 0 0 0 0.09 0.05 0 0 0.06 0.06 0.08
Endosome 0.11 0 0.2 0.13 0.13 0 0 0.05 – 0 0 0 0.08 0.14 0.23 0 0.06 0 0.21 0.13 0.22 0.07 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0
Mitochondria 0.07 0.22 0.11 0.14 0.09 0.41 0.39 0.4 – 0.52 0.47 0.56 0 0 0 0.07 0 0 0.22 0.27 0.26 0.22 0.26 0.23
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.14 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0.09 0.13 0.07 0.08 0.13 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 91 74 24 3 0 2 0 4 – 1 1 0 20 20 7 1 5 1 120 54 122 79 112 87
Bud 13 19 48 10 15 40 44 55 – 33 57 72 5 5 2 0 1 2 28 26 23 54 57 36
Bud Neck 1 2 4 1 0 0 0 1 – 1 0 1 0 0 0 2 0 0 26 32 23 28 24 23
Bud Site 7 6 11 4 4 10 7 5 – 16 10 6 0 0 0 0 1 1 – – – – – –
Cell Periphery 4 0 5 1 0 1 2 0 – 2 1 2 2 4 1 2 3 0 1 1 2 2 8 7
Cytoplasm 206 143 219 206 295 408 400 398 – 331 314 330 149 161 134 52 63 29 59 40 36 26 50 55
Endoplasmic Reticulum 68 63 11 4 3 9 4 3 – 9 1 2 19 42 8 0 3 3 38 13 31 22 37 42
Endosome 47 1 98 39 53 7 15 32 – 3 2 10 17 38 52 1 5 0 151 58 161 25 38 32
Golgi 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 50 18 50 10 13 11
Mitochondria 33 71 54 41 37 250 246 269 – 307 252 369 2 3 0 4 3 1 162 116 188 84 153 115
Nucleus 4 3 2 1 6 0 3 3 – 0 5 1 0 0 0 2 3 1 1 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 1 0 1 – 0 0 1 0 0 0 0 0 0 0 0 2 1 1 2
Nucleolus 1 0 1 0 1 1 1 3 – 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 35 7 12 3 4 8 – 0 0 5 4 5 8 0 0 0 10 4 16 5 2 3
SpindlePole 29 3 71 10 11 10 24 28 – 12 13 16 9 11 18 2 4 1 8 5 9 3 3 4
Vac/Vac Membrane 15 3 22 9 19 10 16 10 – 9 7 12 2 9 15 1 1 0 67 58 48 31 76 73
Unique Cell Count 442 324 498 302 406 604 631 670 585 537 659 212 267 226 61 80 35 737 440 730 379 591 502
Labelled Cell Count 523 389 605 336 456 752 766 820 726 664 830 229 298 245 67 92 39 737 440 730 379 591 502


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 5.0 4.7 4.3 3.5 3.5 3.9 – 3.1 3.3 3.4 6.4 6.4 6.9 5.1 5.3 5.7 6.4 6.4 6.5
Std Deviation (1e-4) 0.7 0.7 1.1 1.5 1.6 0.9 1.0 0.9 – 0.8 0.9 0.8 0.8 0.8 0.9 1.3 1.0 1.6 1.3 1.3 1.4
Intensity Change (Log2) – – – -0.09 -0.2 -0.52 -0.52 -0.36 – -0.66 -0.58 -0.56 0.37 0.36 0.46 0.04 0.1 0.2 0.36 0.36 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.3 1.5 -1.1 0 0 0
Bud 0 0 0 0 0 – 0 0 0 -3.4 -4.0 -4.3 -2.5 -2.5 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 6.7 8.7 7.9 6.5 5.2 – 4.1 4.7 2.1 6.4 4.3 3.8 6.1 5.8 4.5
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 4.1 7.0 1.0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 -3.9 -1.9 1.0 -3.5 -2.9 -2.9
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -4.5 -4.9 -5.1 -1.0 -2.0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 -2.8 -3.1 -1.8 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 -3.9 -4.3 -2.4 -2.4 -2.3 0
Vacuole -1.0 0.2 -2.7 -1.7 -3.0 – -2.8 -3.0 -2.6 -2.3 -0.7 1.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0255 2.078 1.6209 2.0007 1.9182 1.7129 4.5921 3.8028 3.6271 3.2621 4.5612 4.0661 3.5083 3.825 3.6478 4.1425 3.9778 3.9258
Actin 0.0573 0.0212 0.0145 0.0389 0.0098 0.0226 0.0503 0.0089 0.0113 0.0008 0.0554 0.0148 0.0257 0.0084 0.0095 0.011 0.0631 0.0315
Bud 0.0069 0.0082 0.0045 0.0022 0.0263 0.003 0.003 0.0085 0.0077 0.0017 0.0036 0.0117 0.0111 0.0115 0.0055 0.0071 0.0026 0.0031
Bud Neck 0.0222 0.0035 0.0011 0.0037 0.0016 0.0922 0.0178 0.0024 0.0044 0.0003 0.0031 0.1062 0.0346 0.003 0.0007 0.004 0.0554 0.1592
Bud Periphery 0.0055 0.0086 0.0053 0.0054 0.0087 0.0055 0.0058 0.0078 0.0107 0.0026 0.003 0.0112 0.0175 0.007 0.0076 0.0078 0.0027 0.0033
Bud Site 0.0625 0.1328 0.0258 0.0045 0.0077 0.0027 0.0407 0.1051 0.0341 0.0005 0.0035 0.0018 0.0621 0.1238 0.0105 0.0115 0.0229 0.0068
Cell Periphery 0.0014 0.0022 0.0015 0.0009 0.0003 0.0008 0.0021 0.0016 0.0012 0.0001 0.0002 0.0018 0.003 0.002 0.0005 0.0007 0.0004 0.0009
Cytoplasm 0.1899 0.2876 0.3081 0.2338 0.2381 0.3058 0.2325 0.3128 0.2622 0.2644 0.0947 0.3477 0.2786 0.3628 0.3701 0.2683 0.2154 0.3853
Cytoplasmic Foci 0.1376 0.0929 0.1258 0.1247 0.1036 0.1503 0.1222 0.136 0.1061 0.1069 0.1277 0.0829 0.1105 0.087 0.1146 0.1323 0.1105 0.0876
Eisosomes 0.0007 0.0006 0.0004 0.0004 0.0001 0.0002 0.0009 0.0003 0.0003 0.0001 0.0003 0.0002 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0126 0.0079 0.009 0.0174 0.0054 0.0135 0.0142 0.0068 0.0089 0.0088 0.0056 0.0055 0.0186 0.0072 0.0055 0.0097 0.0095 0.0043
Endosome 0.1428 0.108 0.1937 0.3009 0.2283 0.1238 0.1094 0.1025 0.1157 0.3326 0.178 0.0692 0.1861 0.1258 0.2182 0.1981 0.2345 0.0836
Golgi 0.0251 0.0214 0.0284 0.0202 0.0248 0.0338 0.0195 0.0157 0.012 0.0127 0.0243 0.0097 0.0199 0.0241 0.0173 0.0308 0.0384 0.0166
Lipid Particles 0.02 0.0043 0.0051 0.0085 0.0112 0.0117 0.016 0.0024 0.0038 0.0038 0.0264 0.0032 0.0172 0.0034 0.0061 0.0054 0.0076 0.0021
Mitochondria 0.017 0.0144 0.0102 0.0075 0.0089 0.0103 0.0096 0.0102 0.0049 0.0049 0.0048 0.0081 0.0064 0.0076 0.0146 0.019 0.0062 0.0039
None 0.2331 0.2544 0.2375 0.1824 0.2602 0.1934 0.2676 0.2521 0.385 0.2218 0.3883 0.3002 0.1535 0.2034 0.1744 0.2377 0.1952 0.1996
Nuclear Periphery 0.0137 0.0018 0.0032 0.0077 0.0109 0.0016 0.026 0.0021 0.0021 0.0043 0.007 0.0054 0.0163 0.0019 0.0018 0.0021 0.001 0.0006
Nucleolus 0.0019 0.0009 0.0007 0.0005 0.0007 0.0006 0.0017 0.0007 0.0013 0.0002 0.0005 0.0008 0.0007 0.0005 0.0004 0.0018 0.0003 0.0001
Nucleus 0.0118 0.0025 0.0041 0.005 0.0039 0.0019 0.0167 0.0024 0.0035 0.0015 0.0017 0.0074 0.0033 0.0021 0.0017 0.002 0.0006 0.0007
Peroxisomes 0.0161 0.0066 0.0068 0.0069 0.024 0.0168 0.0135 0.0067 0.0082 0.0014 0.0513 0.0021 0.0119 0.0038 0.0217 0.0307 0.0249 0.0048
Punctate Nuclear 0.0117 0.0028 0.0033 0.0107 0.0081 0.0018 0.0117 0.0026 0.0048 0.0013 0.0129 0.0023 0.0016 0.0016 0.0039 0.0047 0.0016 0.0008
Vacuole 0.008 0.0156 0.0088 0.0134 0.0142 0.0062 0.0151 0.0107 0.01 0.0225 0.0055 0.0062 0.0176 0.0114 0.0121 0.0122 0.0048 0.0041
Vacuole Periphery 0.0022 0.0019 0.0025 0.0046 0.0031 0.0014 0.0038 0.0017 0.0018 0.0068 0.0022 0.0016 0.0034 0.0016 0.003 0.0029 0.0022 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2071 11.7071 11.3375 18.7853 13.814 12.2015 16.5017 17.8065 18.307 21.3597
Translational Efficiency 0.4252 0.335 0.4578 0.2852 0.3606 0.4212 0.2949 0.2538 0.3045 0.2606

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
301 915 1500 142 1105 1932 1609 1785 1406 2847 3109 1927

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 851.57 777.08 843.88 869.98 592.89 769.20 775.71 824.67 648.27 771.73 808.60 828.01
Standard Deviation 157.03 112.41 108.52 120.85 97.09 93.98 111.21 105.36 154.75 100.34 115.07 107.23
Intensity Change Log 2 — -0.132062 -0.013087 0.030857 — 0.375594 0.387753 0.476052 — 0.098271 0.165098 0.230457

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.005350 0.005990 0.001237 0.002561 0.010942 0.005364 0.006109 0.006654 0.009745 0.005565 0.003758 0.006352
Bud Neck 0.008319 0.032020 0.018472 0.018417 0.059393 0.044273 0.027940 0.024240 0.048459 0.040335 0.023372 0.023811
Bud Site 0.079352 0.059800 0.028138 0.062313 0.060478 0.044923 0.071523 0.062991 0.064519 0.049704 0.050591 0.062941
Cell Periphery 0.003237 0.000447 0.000293 0.000333 0.004028 0.000328 0.000361 0.000381 0.003859 0.000366 0.000328 0.000377
Cytoplasm 0.299561* 0.179244* 0.313097* 0.241945* 0.130496* 0.196483* 0.350594* 0.394708* 0.166690* 0.190942* 0.332503* 0.383451*
Cytoplasmic Foci 0.126590 0.421344* 0.194383* 0.143221 0.221538* 0.387165* 0.226772* 0.213413* 0.201211* 0.398150* 0.211145* 0.208240*
Eisosomes 0.000240 0.000681 0.000157 0.000198 0.005214 0.001285 0.000216 0.000384 0.004149 0.001091 0.000188 0.000371
Endoplasmic Reticulum 0.003543 0.002686 0.008936 0.004048 0.004497 0.001562 0.006377 0.006293 0.004293 0.001923 0.007612 0.006127
Endosome 0.036650 0.077533 0.068553 0.039776 0.063148 0.076402 0.031547 0.019473 0.057475 0.076766 0.049401 0.020969
Golgi 0.047339 0.084634 0.004453 0.001831 0.137582* 0.086823 0.007186 0.006686 0.118263* 0.086119 0.005868 0.006328
Lipid Particles 0.004042 0.019643 0.042881 0.008003 0.054710 0.023927 0.022217 0.014655 0.043862 0.022550 0.032187 0.014165
Mitochondria 0.010138 0.017923 0.003438 0.000535 0.151724* 0.032776 0.003342 0.002296 0.121413* 0.028003 0.003388 0.002167
Mitotic Spindle 0.018761 0.004359 0.013941 0.007229 0.001697 0.003956 0.023125 0.004787 0.005350 0.004086 0.018694 0.004967
None 0.000195 0.000545 0.007100 0.002128 0.002361 0.000251 0.001763 0.000611 0.001898 0.000346 0.004338 0.000723
Nuclear Periphery 0.000347 0.000130 0.006552 0.000911 0.000285 0.000082 0.001073 0.000384 0.000298 0.000098 0.003716 0.000423
Nuclear Periphery Foci 0.001624 0.000634 0.011147 0.004805 0.000616 0.000217 0.006287 0.002274 0.000832 0.000351 0.008632 0.002461
Nucleolus 0.000200 0.000441 0.005115 0.001578 0.001148 0.000385 0.001160 0.000452 0.000945 0.000403 0.003068 0.000535
Nucleus 0.027648 0.008285 0.102673 0.070223 0.015386 0.008142 0.067626 0.067180 0.018011 0.008188 0.084535 0.067404
Peroxisomes 0.006505 0.034085 0.011306 0.023204 0.054260 0.034119 0.023913 0.019342 0.044037 0.034108 0.017830 0.019627
Vacuole 0.306818* 0.044110 0.155459 0.363893* 0.014107 0.045984 0.119143 0.151741 0.076771 0.045382 0.136664 0.167374
Vacuole Periphery 0.013541 0.005469 0.002669 0.002846 0.006390 0.005551 0.001726 0.001054 0.007921 0.005524 0.002181 0.001186

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.26 2.58 1.91 4.21 -1.24 3.81 3.47 3.15 -1.00 -0.36 3.32 5.14 2.89 -0.45 -3.33
Bud Neck -7.72 -6.50 -2.92 4.20 1.05 3.22 7.42 8.47 7.88 1.46 2.10 7.46 7.36 7.92 -0.04
Bud Site 0.49 4.23 1.16 0.96 -2.05 3.22 -0.89 0.38 -3.41 1.43 2.69 3.20 0.71 -2.17 -2.77
Cell Periphery 1.14 1.24 1.23 2.29 -0.21 8.07 8.03 8.00 -0.84 -0.32 6.47 6.57 6.49 0.35 -1.30
Cytoplasm 7.20 -0.37 3.26 -1.86 4.20 -9.44 -24.49 -31.03 -23.84 -4.83 -3.54 -21.33 -24.85 -25.04 -6.28
Cytoplasmic Foci -27.86 -8.60 -0.70 22.12 5.85 -22.28 0.36 2.10 25.82 1.74 -31.40 -1.36 -0.30 31.75 1.05
Eisosomes -6.81 1.63 0.80 7.57 -0.92 7.86 10.25 9.94 8.13 -6.54 7.73 10.22 9.78 9.17 -8.26
Endoplasmic Reticulum 0.21 -6.80 -1.43 -2.09 5.94 3.35 -2.13 -1.42 -7.52 0.80 3.11 -4.52 -1.93 -7.06 2.87
Endosome -10.10 -8.91 -0.82 7.01 5.64 -3.52 10.07 14.52 22.77 6.20 -6.68 2.86 13.87 25.79 14.64
Golgi -5.76 4.01 4.39 18.89 2.83 8.49 25.01 25.13 26.59 0.41 5.75 24.16 24.04 32.00 -0.60
Lipid Particles -8.82 -14.89 -2.68 5.80 12.36 8.90 9.37 12.13 6.39 4.67 7.61 4.30 11.03 6.86 10.91
Mitochondria -3.34 1.50 2.58 11.05 7.61 18.67* 24.34 24.53 16.27 2.31 17.67 23.14 23.39 18.74 3.74
Mitotic Spindle 2.09 -0.03 0.04 -2.13 0.09 -2.57 -9.82 -3.60 -0.95 8.21 0.30 -8.37 -0.84 -1.60 8.74
None -1.47 -5.12 -1.70 -1.16 2.99 2.58 0.78 2.17 -3.62 1.97 2.33 -2.26 1.89 -1.94 4.99
Nuclear Periphery 4.34 -8.40 -4.82 -6.56 7.19 2.21 -3.59 -1.35 -9.56 3.28 2.81 -9.06 -2.09 -10.69 8.75
Nuclear Periphery Foci 3.61 -10.79 -2.78 -3.30 2.44 1.79 -8.63 -5.57 -9.62 6.33 2.53 -14.09 -6.06 -9.30 10.72
Nucleolus -2.73 -9.81 -2.50 -1.85 5.24 2.13 -0.34 2.09 -0.33 4.19 1.88 -5.73 1.60 -0.86 9.63
Nucleus 5.55 -13.46 -3.47 -6.41 4.38 2.80 -14.44 -14.72 -23.76 0.90 4.68 -21.06 -15.16 -24.71 5.74
Peroxisomes -11.93 -4.27 -2.21 2.87 -1.16 6.92 11.10 13.03 8.46 2.39 4.06 12.07 10.88 9.53 -0.86
Vacuole 18.65 11.50 -2.29 -15.64 -10.77 -14.07 -28.11 -34.20 -25.96 -5.12 7.77 -9.84 -14.06 -28.90 -6.86
Vacuole Periphery 1.38 2.35 2.41 2.66 0.57 1.49 10.96 13.06 10.01 2.15 1.99 6.86 8.10 11.20 4.31
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Dnf3

Dnf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dnf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available