Standard name
Human Ortholog
Description Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0.11 0.1 0.09 0.09 0.09
Bud 0.1 0.11 0.13 0.14 0.12 0.1 0.09 0.07 0.12 0.08 0.14 0.13 0.12 0.08 0 0.06 0.17 0 0.05 0.05 0 0.07 0 0
Bud Neck 0.06 0.06 0.2 0.16 0.15 0.15 0.13 0.11 0.09 0.06 0.08 0.11 0 0 0 0.05 0.06 0 0 0.05 0 0.05 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.59 0.7 0.68 0.72 0.76 0.71 0.73 0.65 0.71 0.6 0.6 0.75 0.77 0.79 0.46 0.33 0.3 0.17 0.22 0.23 0.36 0.41 0.51
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.1 0.11 0.19 0.2 0.11 0.08 0.07 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.17 0 0 0 0.08 0.1 0.08 0.29 0.22 0.31 0.24 0.05 0 0 0.09 0.11 0 0.18 0.16 0.21 0.18 0.26 0.19
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0.06 0.14 0 0 0 0 0 0
SpindlePole 0.19 0.15 0.17 0.19 0.16 0.09 0.13 0.16 0.09 0.08 0.09 0.16 0.1 0.07 0.08 0.25 0.39 0.41 0.25 0.2 0.22 0.1 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 4 39 21 27 4 13 4 6 3 1 5 6 0 2 3 1 0 17 33 47 9 20 20
Bud 23 28 61 62 66 53 68 44 64 42 63 71 28 18 11 5 3 1 12 16 15 8 4 9
Bud Neck 15 17 94 70 85 81 95 64 50 30 35 64 9 0 5 4 1 0 4 16 16 6 11 6
Bud Site 1 2 2 1 1 0 0 3 5 3 5 2 2 1 0 1 0 0 – – – – – –
Cell Periphery 1 0 3 1 4 4 2 1 1 1 2 0 0 0 0 0 0 0 1 0 7 1 4 5
Cytoplasm 128 156 321 291 400 415 518 436 355 355 278 334 172 182 228 36 6 11 46 68 110 39 98 123
Endoplasmic Reticulum 0 5 1 2 0 1 0 0 0 0 0 1 0 0 1 1 0 1 2 0 0 2 2 3
Endosome 11 4 0 2 0 1 0 0 1 0 0 1 5 21 16 8 2 7 55 34 38 7 11 5
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 0 0
Mitochondria 31 45 2 6 17 43 69 49 157 110 146 133 11 5 10 7 2 1 49 48 104 20 62 45
Nucleus 0 0 0 1 4 1 5 4 2 1 2 5 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 1 0 0 2 0 1 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 8 2 1 2 0 0 0 0 2 3 1 1 1 0 0 1 0 0 1 0 0 0 0 0
Peroxisomes 12 7 0 0 0 0 2 0 3 0 0 0 6 18 14 5 1 5 6 9 14 1 2 1
SpindlePole 44 40 80 83 88 49 91 98 49 40 43 90 22 17 22 20 7 15 69 62 107 10 9 6
Vac/Vac Membrane 2 5 0 0 1 2 5 7 0 0 4 6 0 0 0 7 1 1 7 8 8 0 6 7
Unique Cell Count 234 265 461 431 552 544 725 600 544 501 465 561 230 236 288 79 18 37 277 306 491 111 238 239
Labelled Cell Count 285 316 605 543 693 654 870 710 696 589 580 713 264 262 309 100 24 43 277 306 491 111 238 239


Ambiguous, Bud, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.6 4.4 4.0 4.3 3.4 3.6 3.9 3.0 2.9 3.0 3.6 3.9 3.9 4.0 6.0 5.6 6.3 3.5 3.5 4.2
Std Deviation (1e-4) 0.7 0.5 0.6 0.9 0.9 0.8 0.8 0.9 0.7 0.6 0.6 1.0 1.0 1.0 0.9 1.4 0.7 2.1 1.1 1.0 1.7
Intensity Change (Log2) – – – -0.12 -0.01 -0.36 -0.27 -0.15 -0.52 -0.57 -0.55 -0.27 -0.16 -0.16 -0.13 0.47 0.37 0.54 -0.3 -0.3 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6536 -0.9017 -0.5327 -0.9417 -0.089 -0.8223 -0.9814 -1.0553 -0.7768 -0.6085 -0.6196 -1.0032 -0.7669 -1.262 -1.1759 -1.1148 -0.9886 -1.3715
Actin 0.0203 0.0035 0.0327 0.0005 0.0995 0.006 0.0385 0.0088 0.0238 0.0034 0.0159 0.011 0.0536 0.0164 0.0245 0.001 0.005 0.0065
Bud 0.0009 0.0018 0.0036 0.0007 0.0026 0.0005 0.0007 0.0062 0.007 0.0166 0.0133 0.0008 0.0018 0.0022 0.0091 0.0038 0.0011 0.0024
Bud Neck 0.0088 0.0032 0.0267 0.0008 0.0092 0.0187 0.0024 0.0049 0.0159 0.0085 0.0009 0.0101 0.0048 0.0015 0.0264 0.0053 0.0052 0.0413
Bud Periphery 0.003 0.0029 0.012 0.0024 0.0065 0.0011 0.0015 0.0126 0.0223 0.0316 0.0224 0.002 0.0041 0.0057 0.0275 0.0493 0.0117 0.0177
Bud Site 0.0093 0.0194 0.0187 0.0009 0.0164 0.0016 0.0055 0.0339 0.0229 0.0386 0.0064 0.0007 0.0136 0.0296 0.0323 0.0079 0.0032 0.0064
Cell Periphery 0.0014 0.0007 0.0007 0.0002 0.0005 0.0002 0.0005 0.0015 0.0006 0.0009 0.0002 0.0002 0.0028 0.0083 0.0152 0.0329 0.031 0.0179
Cytoplasm 0.0415 0.0247 0.0258 0.0069 0.0058 0.0581 0.0712 0.118 0.0594 0.0279 0.0093 0.0919 0.0451 0.0813 0.0406 0.0171 0.0286 0.1158
Cytoplasmic Foci 0.1944 0.1404 0.2821 0.3646 0.405 0.2303 0.279 0.1654 0.2703 0.3249 0.3541 0.2592 0.1563 0.0876 0.2539 0.3783 0.2149 0.2078
Eisosomes 0.0009 0.0004 0.0013 0 0.0008 0.0003 0.0009 0.0006 0.0004 0.0001 0.0004 0.0002 0.0008 0.0014 0.0007 0.0002 0.0003 0.0004
Endoplasmic Reticulum 0.0126 0.0015 0.0011 0.0003 0.0011 0.001 0.0022 0.0028 0.0017 0.0007 0.0009 0.0014 0.0014 0.0032 0.0013 0.0005 0.0013 0.0026
Endosome 0.0403 0.0123 0.0144 0.037 0.0352 0.0169 0.0355 0.034 0.0179 0.0389 0.0412 0.0252 0.0096 0.0115 0.016 0.049 0.0699 0.0315
Golgi 0.0073 0.0018 0.0051 0.0076 0.0242 0.004 0.009 0.0042 0.0107 0.0218 0.0157 0.0046 0.0062 0.0019 0.009 0.0074 0.0177 0.0064
Lipid Particles 0.0328 0.0171 0.0184 0.0668 0.0613 0.0257 0.0288 0.0119 0.0174 0.0334 0.0803 0.0164 0.0205 0.0103 0.0104 0.0724 0.1559 0.0442
Mitochondria 0.0048 0.0017 0.0115 0.0194 0.009 0.006 0.016 0.0036 0.0151 0.0095 0.0079 0.0125 0.0157 0.0033 0.0179 0.0194 0.0507 0.0072
None 0.5345 0.7031 0.4032 0.1232 0.1004 0.5236 0.4104 0.5359 0.2643 0.1564 0.1118 0.4695 0.6043 0.7106 0.3283 0.0984 0.2464 0.4237
Nuclear Periphery 0.006 0.0011 0.0019 0.0004 0.0026 0.007 0.0041 0.0013 0.0008 0.0011 0.008 0.0043 0.005 0.0006 0.0007 0.0002 0.0033 0.0013
Nucleolus 0.0019 0.0029 0.0015 0.0019 0.002 0.0023 0.0009 0.001 0.0016 0.0012 0.0011 0.0016 0.0024 0.0007 0.0011 0.0016 0.003 0.0017
Nucleus 0.012 0.0015 0.0012 0.0008 0.0012 0.0028 0.0021 0.0017 0.0023 0.0017 0.0016 0.0026 0.0048 0.0008 0.0017 0.0014 0.0014 0.0022
Peroxisomes 0.0512 0.0391 0.1271 0.2912 0.1998 0.0638 0.0742 0.0419 0.2352 0.2671 0.291 0.0631 0.0308 0.0157 0.1724 0.2301 0.1268 0.036
Punctate Nuclear 0.0116 0.0186 0.0068 0.0633 0.0146 0.0279 0.0127 0.0054 0.0076 0.003 0.0144 0.0208 0.0137 0.0031 0.0032 0.0026 0.012 0.0227
Vacuole 0.0038 0.0021 0.0028 0.0091 0.0017 0.0016 0.0029 0.0037 0.0024 0.0108 0.002 0.0016 0.0022 0.0035 0.007 0.0173 0.008 0.0035
Vacuole Periphery 0.0008 0.0003 0.0011 0.002 0.0006 0.0007 0.0012 0.0007 0.0005 0.0018 0.001 0.0005 0.0005 0.0004 0.001 0.0041 0.0024 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5451 10.2456 9.3306 6.8568 9.4564 11.4549 13.891 12.5536 8.8754 11.3281
Translational Efficiency 0.3416 0.4308 0.2632 0.3384 0.3845 0.4168 0.4041 0.3752 0.3831 0.3648

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
257 550 1569 1309 1820 894 1955 266 2077 1444 3524 1575

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 640.05 676.03 890.84 816.79 683.14 729.28 827.91 842.31 677.81 709.00 855.93 821.10
Standard Deviation 64.43 95.04 101.66 108.65 76.58 87.03 94.55 103.27 76.51 93.80 102.66 108.19
Intensity Change Log 2 — 0.078903 0.476982 0.351781 — 0.094292 0.277293 0.302170 — 0.086868 0.377339 0.326381

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001959 0.001435 0.001479 0.002243 0.000934 0.001348 0.000949 0.013804 0.001061 0.001381 0.001185 0.004196
Bud Neck 0.096332 0.055830 0.052339 0.067184 0.072775 0.080545 0.088997 0.045486 0.075690 0.071132 0.072676 0.063519
Bud Site 0.037458 0.030877 0.061054 0.125804* 0.031073 0.034535 0.054636 0.083036 0.031863 0.033142 0.057494 0.118581*
Cell Periphery 0.000651 0.000196 0.000226 0.000198 0.000204 0.000371 0.000189 0.000204 0.000259 0.000305 0.000205 0.000199
Cytoplasm 0.244367* 0.216916* 0.286931* 0.291901* 0.249381* 0.168490* 0.308680* 0.239342* 0.248761* 0.186935* 0.298996* 0.283024*
Cytoplasmic Foci 0.354798* 0.327511* 0.034863 0.053615 0.364998* 0.331149* 0.058516 0.099349 0.363736* 0.329763* 0.047985 0.061339
Eisosomes 0.000989 0.000251 0.000087 0.000101 0.000279 0.000460 0.000098 0.000154 0.000367 0.000380 0.000093 0.000110
Endoplasmic Reticulum 0.000404 0.000646 0.004728 0.001544 0.000531 0.000798 0.001484 0.002360 0.000516 0.000740 0.002929 0.001682
Endosome 0.047291 0.065465 0.011914 0.038903 0.056550 0.090936 0.042380 0.032206 0.055405 0.081235 0.028815 0.037772
Golgi 0.008407 0.013637 0.000843 0.003761 0.011859 0.021719 0.001272 0.002067 0.011432 0.018641 0.001081 0.003475
Lipid Particles 0.026375 0.014852 0.003143 0.002656 0.015795 0.014427 0.003710 0.004299 0.017104 0.014589 0.003458 0.002934
Mitochondria 0.013258 0.027204 0.001906 0.005107 0.012901 0.032091 0.003343 0.000925 0.012945 0.030229 0.002704 0.004401
Mitotic Spindle 0.000676 0.006469 0.026661 0.068230 0.003932 0.014896 0.034551 0.034496 0.003529 0.011687 0.031038 0.062533
None 0.001185 0.003182 0.005233 0.002882 0.002130 0.001120 0.004558 0.003656 0.002013 0.001905 0.004859 0.003013
Nuclear Periphery 0.000599 0.000637 0.002144 0.001049 0.000194 0.000423 0.001504 0.000877 0.000244 0.000505 0.001789 0.001020
Nuclear Periphery Foci 0.000221 0.000486 0.002160 0.001608 0.000356 0.000417 0.001272 0.003468 0.000340 0.000443 0.001667 0.001922
Nucleolus 0.000791 0.003053 0.003738 0.003518 0.001825 0.001610 0.006790 0.002627 0.001697 0.002160 0.005431 0.003367
Nucleus 0.048114 0.056311 0.407412* 0.115051 0.057408 0.031605 0.236413* 0.133294* 0.056258 0.041016 0.312547* 0.118132
Peroxisomes 0.085367 0.108529* 0.022333 0.033891 0.082047 0.121711* 0.041513 0.105209* 0.082458 0.116690* 0.032973 0.045936
Vacuole 0.029332 0.063801 0.070335 0.174676 0.033917 0.048202 0.108290 0.192762 0.033350 0.054144 0.091391 0.177730
Vacuole Periphery 0.001426 0.002712 0.000469 0.006077 0.000911 0.003144 0.000856 0.000378 0.000975 0.002979 0.000683 0.005115

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.81 -0.23 0.04 -1.68 0.69 -1.61 -0.67 -2.42 -2.22 -2.38 -1.48 -2.85 -3.77 -2.94 -2.77
Bud Neck 3.07 3.13 3.55 0.78 1.27 -1.33 -0.67 6.14 6.47 6.66 0.98 2.10 6.32 5.06 5.24
Bud Site 0.90 -2.32 -4.24 -8.03 -3.24 -1.04 -4.95 -3.21 -2.67 -1.18 -0.38 -6.77 -9.02 -8.66 -4.64
Cell Periphery 2.44 2.22 2.60 1.29 5.23 -1.99 1.62 0.36 2.11 -1.18 -0.73 2.04 3.00 2.58 3.01
Cytoplasm 1.38 0.31 -1.12 -3.42 -2.50 8.67 -2.80 2.10 -2.75 3.57 7.40 -1.36 -0.73 -7.60 0.44
Cytoplasmic Foci 1.36 20.43 19.94 29.08 -2.21 3.57 51.09 30.61 23.71 -1.95 4.17 57.47 54.45 43.62 -0.84
Eisosomes 2.23 2.63 2.64 7.32 0.44 -2.61 11.43 5.95 4.27 -3.95 -0.19 5.83 5.63 6.01 -1.54
Endoplasmic Reticulum -1.76 -21.39 -13.28 -12.30 13.01 -0.93 -11.76 -7.67 -6.46 -4.17 -1.17 -22.01 -14.36 -10.32 6.43
Endosome -1.95 4.69 3.32 8.09 -4.24 -6.16 7.45 9.30 12.75 3.56 -5.77 11.37 10.90 15.14 0.76
Golgi -2.25 5.79 5.07 7.42 -2.10 -5.01 13.68 12.80 11.58 0.24 -4.82 14.70 12.89 13.29 -1.87
Lipid Particles 1.89 3.63 3.77 6.97 2.96 0.82 11.44 10.38 6.60 -0.27 1.72 11.12 11.75 10.09 3.02
Mitochondria -2.49 2.80 2.59 6.85 -1.66 -5.71 9.36 11.46 9.81 6.67 -6.56 9.94 9.48 11.47 0.06
Mitotic Spindle -4.49 -11.98 -11.88 -9.89 -3.28 -4.10 -10.59 -3.86 -2.09 0.72 -4.53 -14.53 -11.57 -8.62 -2.84
None -2.17 -6.35 -2.47 0.78 4.27 2.03 -3.97 -2.12 -3.10 0.08 0.25 -6.53 -1.86 -1.55 3.60
Nuclear Periphery -0.08 -8.28 -6.08 -5.48 4.44 -1.24 -23.23 -9.63 -5.48 2.95 -1.60 -27.43 -20.90 -9.31 4.06
Nuclear Periphery Foci -2.67 -10.10 -3.53 -3.15 1.14 -0.82 -7.61 -4.46 -4.33 -2.36 -1.62 -12.33 -4.19 -4.00 -0.40
Nucleolus -4.18 -8.22 -4.26 -0.50 3.39 0.91 -6.71 -1.17 -1.36 1.73 -0.81 -8.68 -2.57 -2.23 4.70
Nucleus -1.11 -34.74 -13.25 -14.00 24.23 7.19 -30.40 -8.09 -9.90 6.46 4.41 -47.97 -18.42 -20.71 21.94
Peroxisomes -1.62 6.17 6.35 11.21 0.74 -5.55 12.68 4.53 7.77 -1.44 -5.71 15.60 15.05 17.68 1.11
Vacuole -6.42 -16.09 -31.80 -25.07 -20.99 -4.48 -26.02 -16.20 -15.20 -9.11 -7.41 -30.45 -36.70 -32.09 -20.62
Vacuole Periphery -2.17 2.10 -0.98 0.31 -2.23 -4.38 2.37 11.47 5.63 3.23 -5.73 4.46 -1.47 1.20 -2.05
Standard name
Human Ortholog
Description Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Inp2

Inp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Inp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available