Standard name
Human Ortholog
Description Transporter of the mitochondrial inner membrane that exports magnesium; involved in mitochondrial Mg2+ homeostasis; has similarity to human mitochondrial ATP-Mg/Pi carriers
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.05 0 0 0 0.05 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.66 0.66 0.64 0.52 0.5 0.46 0.5 0.48 0.5 0.39 0.39 0.91 0.91 0.92 0.78 0.64 0.7 0.11 0.11 0.12 0.07 0.11 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.11 0.08 0.06 0.08 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.35 0.32 0.39 0.49 0.64 0.64 0.57 0.64 0.6 0.77 0.74 0 0 0 0.08 0.09 0.06 0.6 0.53 0.62 0.64 0.59 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.05 0.05 0 0.05 0 0.05 0.07 0 0.06 0 0 0 0.06 0.08 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0.08 0.07 0.11 0 0.07 0.11 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 1 0 0 1 0 0 0 0 0 0 4 4 0 0 1 0 5 8 7 6 8 11
Bud 0 8 15 10 14 41 21 34 39 33 27 49 2 2 0 0 0 2 3 3 1 2 6 6
Bud Neck 0 0 2 1 3 15 13 5 10 3 5 2 3 7 1 1 5 0 4 3 1 6 7 9
Bud Site 0 0 1 1 0 12 11 9 4 9 5 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 2 1 1 3 1 1 1 1 1 0 0 1 1 2 2 0 1 3 2 2 3
Cytoplasm 117 116 230 198 296 410 445 474 356 442 247 377 212 272 276 51 74 74 35 28 52 18 36 33
Endoplasmic Reticulum 6 0 2 0 3 0 2 1 2 0 2 2 5 5 0 1 8 9 4 3 8 6 5 6
Endosome 11 7 2 9 20 2 0 8 0 4 0 0 5 5 11 3 9 12 26 15 35 14 12 11
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 1 0 3 0 2
Mitochondria 59 62 112 122 282 525 618 543 473 530 494 715 2 2 0 5 10 6 188 138 267 168 193 188
Nucleus 3 2 1 1 2 5 14 11 2 1 3 7 2 0 0 2 2 0 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 1 1 1 0 1 0 0 0 0 0 0 2 0 1 0 0 0
Nucleolus 0 0 0 0 2 0 1 4 1 1 1 3 0 0 0 1 0 1 1 2 0 0 1 1
Peroxisomes 0 0 0 4 2 2 1 2 0 4 0 2 0 2 5 0 1 4 2 0 4 2 0 2
SpindlePole 6 2 17 15 31 33 51 41 37 61 21 62 1 4 1 4 9 7 6 5 13 3 4 4
Vac/Vac Membrane 29 8 4 4 20 10 2 2 7 9 5 1 6 9 14 0 11 8 22 29 10 19 37 36
Unique Cell Count 212 177 348 310 570 817 966 946 742 885 639 966 233 299 300 65 116 105 315 263 430 265 329 326
Labelled Cell Count 235 207 388 367 676 1057 1183 1136 933 1099 811 1233 242 312 310 70 132 125 315 263 430 265 329 326


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.0 3.2 3.3 3.3 3.1 2.5 2.8 2.5 2.5 2.4 2.4 5.1 5.0 5.1 4.9 5.0 5.2 5.5 5.7 5.4
Std Deviation (1e-4) 0.6 0.9 0.8 1.2 1.4 1.3 1.0 1.2 1.0 0.8 0.9 0.6 1.0 1.1 1.5 1.6 1.1 1.6 2.2 2.0 1.6
Intensity Change (Log2) – – – 0.03 0.04 -0.07 -0.37 -0.19 -0.36 -0.34 -0.39 -0.4 0.67 0.64 0.66 0.62 0.65 0.71 0.79 0.83 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2871 0.2227 0.0605 0.0799 -0.3305 0.2195 1.1753 1.189 1.5329 0.8545 1.1473 1.3957 -0.866 -0.4022 -0.5361 -0.7511 -0.7778 -0.8224
Actin 0.0147 0.0044 0.0184 0.0041 0.025 0.0079 0.0115 0.0037 0.0173 0.0026 0.0052 0.0022 0.0051 0.0033 0.0069 0.0082 0.0149 0.0073
Bud 0.0011 0.0067 0.0042 0.0024 0.0085 0.0015 0.0006 0.0055 0.0022 0.0061 0.0058 0.0006 0.0015 0.0018 0.0046 0.0079 0.0044 0.0013
Bud Neck 0.012 0.002 0.0028 0.0185 0.0089 0.0112 0.0008 0.0038 0.0039 0.0012 0.0014 0.0029 0.003 0.001 0.0074 0.0027 0.0008 0.0103
Bud Periphery 0.003 0.0073 0.0097 0.0095 0.0289 0.0051 0.0012 0.0325 0.0068 0.0205 0.0302 0.0023 0.0062 0.0043 0.0117 0.026 0.0186 0.0042
Bud Site 0.012 0.0316 0.0237 0.0146 0.0031 0.001 0.0017 0.0374 0.006 0.0031 0.0055 0.0009 0.0045 0.0181 0.0266 0.0051 0.0015 0.0018
Cell Periphery 0.0031 0.0018 0.0013 0.001 0.0012 0.0009 0.0015 0.0072 0.0019 0.0024 0.0037 0.0014 0.0021 0.0018 0.0034 0.0025 0.0006 0.0016
Cytoplasm 0.1048 0.2059 0.1308 0.1407 0.1812 0.1595 0.1909 0.208 0.1247 0.0902 0.1138 0.2314 0.112 0.155 0.1207 0.1403 0.1666 0.1507
Cytoplasmic Foci 0.1109 0.1055 0.1238 0.0689 0.1106 0.1195 0.1469 0.0646 0.103 0.1349 0.0827 0.1177 0.1536 0.1365 0.1286 0.0999 0.1123 0.1411
Eisosomes 0.001 0.0008 0.0009 0.0005 0.0005 0.0005 0.001 0.0012 0.0017 0.0009 0.0014 0.0017 0.001 0.0011 0.0014 0.001 0.0009 0.0004
Endoplasmic Reticulum 0.0107 0.01 0.0052 0.0056 0.0275 0.0075 0.02 0.0225 0.0048 0.0039 0.005 0.0054 0.0099 0.0046 0.0048 0.0105 0.0053 0.006
Endosome 0.16 0.1068 0.1081 0.1006 0.121 0.0876 0.1232 0.1009 0.0594 0.2015 0.0833 0.0608 0.1262 0.0885 0.0906 0.1073 0.18 0.1189
Golgi 0.0352 0.0183 0.0265 0.0153 0.0365 0.0154 0.0366 0.0318 0.0287 0.0385 0.022 0.0156 0.0263 0.0237 0.0244 0.0221 0.0337 0.0319
Lipid Particles 0.0517 0.0121 0.0156 0.028 0.0163 0.024 0.0335 0.007 0.0187 0.0144 0.0197 0.0171 0.0257 0.0116 0.0098 0.0116 0.0136 0.015
Mitochondria 0.0579 0.052 0.1028 0.1484 0.0928 0.1 0.091 0.2062 0.1297 0.2178 0.3005 0.2027 0.1284 0.1551 0.2125 0.192 0.1854 0.1992
None 0.3385 0.3741 0.365 0.3821 0.2663 0.3991 0.2791 0.2352 0.4438 0.2073 0.2822 0.3034 0.3382 0.3472 0.2988 0.2688 0.1953 0.2483
Nuclear Periphery 0.0052 0.0032 0.0016 0.0022 0.0082 0.0029 0.0245 0.0026 0.0011 0.0022 0.0018 0.001 0.005 0.0013 0.0016 0.0059 0.0011 0.0012
Nucleolus 0.0037 0.0022 0.0028 0.0042 0.0008 0.0018 0.0003 0.0011 0.001 0.001 0.001 0.0006 0.0035 0.0012 0.0015 0.0035 0.0004 0.0008
Nucleus 0.0048 0.0054 0.0037 0.0039 0.0025 0.0045 0.0011 0.0023 0.0013 0.002 0.0017 0.0011 0.0049 0.0022 0.0021 0.0058 0.0011 0.0018
Peroxisomes 0.0411 0.0205 0.0352 0.0302 0.0472 0.0311 0.0244 0.0022 0.0344 0.0158 0.0121 0.0227 0.0236 0.0266 0.0286 0.0407 0.0499 0.0422
Punctate Nuclear 0.0053 0.0088 0.0034 0.0018 0.0019 0.0051 0.0008 0.0007 0.0013 0.0035 0.0018 0.0016 0.0039 0.0014 0.0016 0.0032 0.0014 0.0013
Vacuole 0.0199 0.0166 0.0114 0.0116 0.008 0.0104 0.0057 0.0189 0.0059 0.0222 0.0119 0.0045 0.0121 0.0107 0.0092 0.0232 0.0079 0.0101
Vacuole Periphery 0.0033 0.004 0.0032 0.006 0.0031 0.0037 0.0037 0.0046 0.0022 0.0084 0.0073 0.0022 0.0033 0.0032 0.003 0.0118 0.0045 0.0047

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4626 10.6924 7.9342 10.8355 10.9848 12.9506 14.8653 14.2895 12.6962 14.5729
Translational Efficiency 0.6825 0.4862 0.6509 0.5317 0.5228 0.553 0.7697 0.4491 0.6087 0.6451

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
467 848 368 1576 1904 2111 1571 1797 2371 2959 1939 3373

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 843.47 899.71 1264.46 1012.30 796.33 893.99 985.46 992.23 805.61 895.63 1038.41 1001.61
Standard Deviation 122.42 165.67 173.61 188.30 126.79 146.91 138.87 185.98 127.33 152.54 182.52 187.34
Intensity Change Log 2 — 0.093123 0.584113 0.263228 — 0.166892 0.307431 0.317308 — 0.129419 0.456354 0.289744

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.000155 0.000277 0.000298 0.000099 0.000124 0.000055 0.000407 0.000106 0.000133 0.000097 0.000356
Bud Neck 0.004164 0.003883 0.011097 0.009544 0.002234 0.004392 0.003123 0.007219 0.002614 0.004246 0.004636 0.008305
Bud Site 0.001611 0.001833 0.002099 0.006836 0.001276 0.002452 0.000915 0.005087 0.001342 0.002275 0.001139 0.005904
Cell Periphery 0.000116 0.000147 0.000077 0.000145 0.000125 0.000140 0.000044 0.000119 0.000123 0.000142 0.000050 0.000131
Cytoplasm 0.005831 0.002511 0.000254 0.012328 0.002891 0.002621 0.000358 0.017833 0.003470 0.002589 0.000338 0.015261
Cytoplasmic Foci 0.031209 0.019836 0.038027 0.041068 0.021209 0.032943 0.013816 0.064639 0.023179 0.029187 0.018411 0.053626
Eisosomes 0.000165 0.000197 0.000368 0.000035 0.000085 0.000155 0.000060 0.000026 0.000101 0.000167 0.000119 0.000030
Endoplasmic Reticulum 0.003125 0.002669 0.001416 0.001939 0.002106 0.001502 0.000694 0.001960 0.002307 0.001837 0.000831 0.001950
Endosome 0.012366 0.011673 0.003196 0.033152 0.016495 0.010887 0.006516 0.053673 0.015682 0.011112 0.005886 0.044085
Golgi 0.001786 0.003032 0.001793 0.001622 0.002742 0.002031 0.000765 0.003235 0.002553 0.002318 0.000960 0.002481
Lipid Particles 0.020620 0.036393 0.006029 0.083268 0.035198 0.032785 0.019732 0.118264* 0.032326 0.033819 0.017131 0.101913*
Mitochondria 0.000675 0.001339 0.001146 0.001446 0.001944 0.001019 0.000126 0.002231 0.001694 0.001111 0.000320 0.001864
Mitotic Spindle 0.013257 0.042310 0.183914* 0.287252* 0.011897 0.055454 0.153608* 0.184672* 0.012165 0.051687 0.159360* 0.232602*
None 0.000995 0.001604 0.000262 0.000717 0.003038 0.001698 0.000179 0.001161 0.002636 0.001671 0.000195 0.000953
Nuclear Periphery 0.011296 0.009141 0.017267 0.022067 0.005827 0.005070 0.009492 0.016394 0.006904 0.006236 0.010967 0.019045
Nuclear Periphery Foci 0.758025* 0.753895* 0.193912* 0.461743* 0.813102* 0.752497* 0.742135* 0.492148* 0.802254* 0.752897* 0.638088* 0.477941*
Nucleolus 0.093062 0.075889 0.423632* 0.011410 0.055768 0.064593 0.035209 0.008027 0.063114 0.067830 0.108927 0.009608
Nucleus 0.026954 0.020226 0.062354 0.008589 0.012349 0.016972 0.006531 0.006842 0.015226 0.017904 0.017125 0.007658
Peroxisomes 0.011696 0.009246 0.043641 0.003626 0.009350 0.010103 0.003817 0.004462 0.009812 0.009857 0.011376 0.004072
Vacuole 0.002505 0.003222 0.007782 0.011424 0.001844 0.002187 0.002638 0.010440 0.001974 0.002484 0.003614 0.010900
Vacuole Periphery 0.000409 0.000799 0.001454 0.001490 0.000419 0.000375 0.000187 0.001162 0.000417 0.000497 0.000428 0.001315

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.71 -4.53 -2.04 -1.84 -0.55 -1.97 3.08 -1.55 -1.41 -1.74 -2.28 0.34 -2.30 -2.05 -2.33
Bud Neck 0.28 -5.60 -6.92 -6.83 0.38 -5.89 -3.15 -9.28 -5.28 -6.73 -4.60 -5.76 -13.23 -9.00 -7.52
Bud Site -0.12 -1.36 -6.37 -5.83 -5.98 -3.84 0.41 -6.74 -4.72 -7.00 -3.28 0.00 -9.28 -7.43 -9.39
Cell Periphery -0.70 2.34 -1.44 -0.26 -3.67 -0.87 6.32 0.15 1.12 -8.51 -1.05 6.75 -0.93 0.28 -7.86
Cytoplasm 1.13 2.10 -4.03 -7.05 -8.59 0.38 3.87 -8.56 -8.77 -9.76 1.05 4.22 -10.29 -11.32 -12.89
Cytoplasmic Foci 2.46 -0.92 -2.11 -7.42 -1.09 -4.31 3.70 -13.65 -9.17 -17.19 -2.56 2.51 -12.80 -10.23 -16.40
Eisosomes -1.11 -6.47 7.67 7.28 13.03 -7.88 4.34 11.65 16.28 9.24 -6.84 -2.09 13.11 15.96 13.83
Endoplasmic Reticulum 0.65 2.75 1.22 0.93 -1.74 1.77 4.44 -0.05 -2.02 -4.96 1.50 5.21 0.35 -1.13 -5.16
Endosome 0.23 4.49 -7.72 -8.99 -15.32 3.91 6.91 -14.36 -18.07 -20.24 3.66 7.97 -15.26 -20.06 -24.18
Golgi -2.35 0.47 1.29 2.98 1.15 2.22 6.99 -0.34 -2.09 -5.39 1.01 7.00 0.97 0.04 -5.67
Lipid Particles -3.68 5.31 -11.45 -7.80 -17.01 0.99 5.47 -14.69 -15.57 -18.75 -0.33 6.45 -16.99 -17.15 -22.87
Mitochondria -1.46 -1.48 -1.54 -0.27 -0.71 1.57 3.35 -0.29 -1.88 -3.62 1.20 3.11 -0.31 -1.73 -4.00
Mitotic Spindle -4.95 -11.91 -28.58* -24.00* -6.16 -12.52 -21.15* -25.22* -16.53 -3.08 -13.44 -24.21 -38.56 -28.31* -8.50
None -1.24 3.72 0.39 1.47 -3.21 1.96 4.61 2.58 0.87 -3.09 1.75 4.89 2.94 1.67 -4.13
Nuclear Periphery 0.53 -1.64 -3.17 -5.83 -1.86 0.76 -3.63 -6.94 -8.18 -4.79 0.55 -3.76 -9.07 -11.08 -6.77
Nuclear Periphery Foci 0.17 28.51* 18.11* 22.43* -15.40 6.97 7.77 31.33* 24.57* 22.22* 6.40 16.55 39.27 33.77* 16.42
Nucleolus 1.87 -18.40 9.92 11.50 25.86* -1.90 4.84 14.17 16.71 9.80 -1.04 -7.72 16.93 20.05 19.48
Nucleus 1.49 -5.53 3.11 2.08 8.69 -2.65 3.58 1.56 4.11 -1.80 -1.68 -1.35 2.13 3.95 3.47
Peroxisomes 1.60 -10.25 5.25 5.98 15.08 -0.80 4.88 3.99 6.61 -1.42 -0.14 -1.43 5.97 9.11 9.81
Vacuole -1.74 -6.19 -8.30 -7.93 -5.21 -1.58 -3.10 -9.14 -8.87 -8.16 -2.56 -5.62 -12.37 -11.92 -10.46
Vacuole Periphery -1.18 -4.87 -2.78 -1.33 -0.00 0.43 2.64 -3.59 -3.93 -5.05 -0.66 -0.30 -4.17 -3.54 -4.18
Standard name
Human Ortholog
Description Transporter of the mitochondrial inner membrane that exports magnesium; involved in mitochondrial Mg2+ homeostasis; has similarity to human mitochondrial ATP-Mg/Pi carriers
Localization
Cell Percentages spindle pole (12%), nucleolus (10%), mixed (38%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mme1

Mme1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mme1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available