Standard name
Human Ortholog
Description Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 – 0 0.07 0.05 0.09 0 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0.05 0 0.1 0.05 0.14 0.05 0.07 – 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0.09 0.08 0.06 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.29 0.31 0.54 0.37 0.42 0.33 0.34 – 0.21 0.16 0.15 0.19 0.32 0.35 0.38 0.75 0.71 – 0.15 0.13 0.17 0.17 0.12 0.11
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Endosome 0.06 0.17 0.06 0.07 0.06 0.13 0.06 – 0 0 0 0 0.1 0.38 0.34 0.05 0.11 – 0.21 0.18 0.13 0.16 0.21 0.2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.3 0.17 0 0.1 0 0.34 0.33 – 0.64 0.7 0.72 0.5 0.42 0.07 0.1 0.11 0.05 – 0.05 0.1 0.12 0.09 0.1 0.11
Nucleus 0.09 0.09 0.06 0.08 0 0.08 0.07 – 0.06 0.1 0.07 0.12 0.09 0 0.05 0 0 – 0.06 0.07 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.05 0 0 – 0 0 0 0 0 0
SpindlePole 0.13 0.1 0.23 0.39 0.36 0.21 0.3 – 0.19 0.23 0.16 0.31 0.15 0.19 0.12 0.13 0.16 – 0.33 0.26 0.29 0.22 0.3 0.31
Vac/Vac Membrane 0.15 0.22 0.05 0 0 0 0 – 0 0 0 0 0.05 0.1 0.09 0.05 0.06 – 0.08 0.12 0.11 0.19 0.13 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 3 0 0 – 0 0 0 0 1 0 1 0 1 – 1 2 1 1 2 3
Bud 0 2 0 8 4 4 9 – 11 18 13 22 0 1 4 2 2 – 1 3 2 2 2 4
Bud Neck 4 4 18 8 24 11 19 – 8 7 9 11 0 1 0 11 10 – 0 2 2 0 3 5
Bud Site 0 0 3 2 1 7 8 – 10 23 21 15 1 0 1 0 1 – – – – – – –
Cell Periphery 0 0 0 0 0 1 0 – 0 0 1 0 0 0 0 4 7 – 0 0 0 0 0 0
Cytoplasm 25 72 102 62 72 73 96 – 55 39 39 46 35 44 65 495 422 – 24 48 46 23 21 21
Endoplasmic Reticulum 5 6 1 0 4 2 0 – 0 0 0 0 0 1 0 35 25 – 0 0 0 3 1 1
Endosome 5 40 11 11 10 29 18 – 7 0 7 9 11 48 59 32 63 – 32 63 36 22 36 38
Golgi 0 0 3 0 0 0 0 – 0 0 0 0 0 1 0 1 1 – 0 0 1 0 0 0
Mitochondria 26 40 4 17 7 76 94 – 164 172 185 118 46 9 18 73 29 – 8 37 33 13 17 20
Nucleus 8 21 12 14 6 18 19 – 15 25 18 28 10 5 8 8 5 – 9 25 9 4 2 0
Nuclear Periphery 1 1 0 0 0 0 0 – 1 0 2 0 0 0 0 1 5 – 2 3 2 0 1 2
Nucleolus 2 3 0 2 0 2 4 – 3 6 4 5 3 3 7 8 4 – 3 10 4 4 2 1
Peroxisomes 0 0 0 0 0 1 3 – 2 0 0 1 0 7 8 1 1 – 0 1 0 0 0 1
SpindlePole 11 24 44 65 63 48 86 – 49 55 42 72 16 24 20 84 98 – 52 94 79 31 53 58
Vac/Vac Membrane 13 50 9 4 5 5 8 – 5 2 6 3 6 13 16 36 34 – 12 42 30 27 23 23
Unique Cell Count 87 229 189 168 173 224 286 257 244 256 236 110 126 172 664 597 160 359 276 143 177 190
Labelled Cell Count 100 263 208 194 199 277 364 330 347 347 330 129 157 207 791 708 160 359 276 143 177 190


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 4.8 4.4 4.9 3.9 4.1 – 3.6 3.3 3.2 3.9 3.7 4.0 4.3 5.2 5.4 4.8 3.6 3.7 4.4
Std Deviation (1e-4) 0.4 0.6 1.3 1.6 1.2 1.4 1.4 – 1.3 1.1 1.1 1.3 0.8 0.9 1.5 1.1 1.2 1.0 1.3 1.2 1.9
Intensity Change (Log2) – – – -0.13 0.01 -0.31 -0.25 – -0.44 -0.54 -0.58 -0.31 -0.38 -0.28 -0.17 0.11 0.16 -0.02 -0.41 -0.39 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 0.4 0.5 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0527 -0.3919 -0.5816 -0.5459 -0.9043 -0.639 -0.4374 -0.323 -0.4093 -0.9684 -0.9442 -0.5125 0.1095 -0.2633 -0.1763 0.029 -0.0724 -0.4711
Actin 0.0359 0.0031 0.0173 0.0039 0.0009 0.0208 0.0147 0.0015 0.0242 0.0025 0.0006 0.0015 0.0153 0.0018 0.0112 0.0204 0.0026 0.004
Bud 0.0008 0.0002 0.0001 0.0002 0.0001 0.0023 0.0005 0.0064 0.0003 0.0002 0.0001 0.0002 0.0007 0.0002 0.0003 0.0006 0.0003 0.0002
Bud Neck 0.0021 0.0017 0.002 0.0031 0.001 0.0054 0.0024 0.0019 0.0016 0.0035 0.0026 0.0067 0.0036 0.0018 0.0027 0.0033 0.0014 0.0066
Bud Periphery 0.0013 0.0004 0.0002 0.0003 0.0004 0.0056 0.0008 0.0012 0.0003 0.0002 0.0001 0.0002 0.0012 0.0002 0.0004 0.0013 0.001 0.0003
Bud Site 0.0056 0.003 0.0013 0.0009 0.0002 0.001 0.0045 0.004 0.004 0.0007 0.0004 0.0005 0.0039 0.0032 0.0072 0.0116 0.0005 0.0004
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0002 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0.0008 0.0001 0.0001
Cytoplasm 0.0561 0.0728 0.0537 0.169 0.0536 0.0472 0.0455 0.0675 0.035 0.0565 0.0747 0.0584 0.0626 0.0821 0.0519 0.1153 0.0794 0.0608
Cytoplasmic Foci 0.0705 0.0477 0.0375 0.0526 0.0225 0.0418 0.0767 0.0601 0.0642 0.0255 0.0389 0.0271 0.0765 0.0593 0.0404 0.0331 0.0266 0.0195
Eisosomes 0.0007 0.0001 0.0001 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0.0014 0.0001 0
Endoplasmic Reticulum 0.0097 0.0063 0.0056 0.0203 0.0106 0.0026 0.0044 0.0045 0.0041 0.0063 0.0133 0.0054 0.0066 0.0047 0.0044 0.0047 0.0052 0.0041
Endosome 0.058 0.0336 0.0208 0.0321 0.0424 0.0144 0.0415 0.0264 0.0239 0.0161 0.0744 0.0254 0.0611 0.0314 0.0241 0.0224 0.0447 0.0182
Golgi 0.0135 0.0031 0.0025 0.0029 0.002 0.0028 0.0067 0.0025 0.0044 0.0014 0.0024 0.0019 0.0118 0.0036 0.0028 0.0033 0.005 0.0018
Lipid Particles 0.0518 0.0393 0.0215 0.0073 0.0354 0.0392 0.0269 0.0346 0.0348 0.0071 0.0286 0.0209 0.0654 0.0259 0.0212 0.0228 0.0094 0.0117
Mitochondria 0.0326 0.0041 0.004 0.0029 0.0061 0.0015 0.0018 0.0016 0.0012 0.0012 0.0005 0.001 0.0337 0.0014 0.0008 0.0069 0.0235 0.0009
None 0.115 0.1812 0.117 0.2583 0.1757 0.1453 0.0449 0.1434 0.0568 0.0288 0.1684 0.0999 0.1364 0.1575 0.1459 0.0572 0.1797 0.0809
Nuclear Periphery 0.0597 0.0515 0.0925 0.0512 0.1678 0.0704 0.0664 0.0606 0.0595 0.0709 0.0423 0.0594 0.0584 0.0308 0.0851 0.0238 0.0535 0.0439
Nucleolus 0.0086 0.0239 0.0201 0.0203 0.0075 0.0181 0.0081 0.0254 0.0195 0.0143 0.0793 0.0294 0.0095 0.0248 0.0223 0.0236 0.0054 0.0208
Nucleus 0.0738 0.1785 0.1619 0.2576 0.1294 0.2419 0.1285 0.13 0.1094 0.3286 0.2373 0.2606 0.0722 0.1375 0.1291 0.3789 0.2798 0.2024
Peroxisomes 0.0274 0.0028 0.0061 0.0011 0.0016 0.0103 0.0046 0.0046 0.0057 0.0065 0.0057 0.0014 0.0164 0.0046 0.0054 0.0208 0.0018 0.0015
Punctate Nuclear 0.3704 0.3402 0.4312 0.1086 0.3235 0.3258 0.5158 0.418 0.5481 0.4218 0.22 0.396 0.3411 0.4226 0.4384 0.2439 0.2672 0.5172
Vacuole 0.0033 0.0051 0.0033 0.0053 0.011 0.0021 0.003 0.0036 0.0018 0.0063 0.0079 0.0028 0.0165 0.0046 0.0047 0.0028 0.0065 0.0035
Vacuole Periphery 0.0026 0.0014 0.0012 0.0019 0.008 0.0009 0.0016 0.0018 0.0009 0.0016 0.0023 0.0013 0.0066 0.0017 0.0014 0.0011 0.006 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9261 4.697 5.0697 8.218 8.0511 5.2514 9.8221 8.5962 6.7687 9.0152
Translational Efficiency 0.8608 0.6515 1.1311 0.6986 0.6071 0.9348 0.5704 0.5222 0.8725 0.6437

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer
Localization
Cell Percentages nucleus (38%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mlh1

Mlh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available