Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.09 0.1 0.11 0.08 0.05 0.05 0.05 0.06 0.05 0.05 – 0.17 0.26 0.09 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.31 0.25 0.18 0.2 0.17 0.13 0.14 0.08 0.13 0.07 0.05 0.11 – 0.21 0.19 0.2 0.25 0.28 0.16 0.16 0.13 0.22 0.21 0.21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.12 0 0.06 0 0.13 0.13 0.12 0.32 0.21 0.42 0.32 – 0 0 0.1 0.06 0.08 0 0 0 0 0 0
Nucleus 0.22 0.23 0.27 0.18 0.22 0.23 0.2 0.21 0.2 0.26 0.17 0.22 – 0.29 0.3 0.36 0.25 0.3 0.1 0.13 0.1 0.1 0.14 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.35 0.29 0.37 0.42 0.44 0.47 0.42 0.41 0.43 0.48 0.42 – 0.25 0.18 0.34 0.41 0.41 0.07 0.17 0.08 0.09 0.13 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.36 0.16 0.49 0.42 0.55 0.35 0.43 0.51 0.32 0.35 0.22 0.32 – 0.17 0.24 0.22 0.31 0.31 0.55 0.41 0.51 0.47 0.35 0.23
Vac/Vac Membrane 0.11 0.14 0.1 0.05 0 0 0 0 0 0 0 0 – 0 0 0.07 0.07 0.1 0 0 0.05 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 1 0 0 1
Bud 2 3 0 0 1 1 4 3 7 10 6 11 – 1 0 0 5 0 1 2 3 0 1 2
Bud Neck 0 0 2 0 2 0 2 2 3 7 2 3 – 0 0 0 1 0 0 3 1 0 1 1
Bud Site 0 1 0 1 1 4 8 11 10 9 13 9 – 0 0 1 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 15 14 16 24 21 16 24 15 15 17 14 – 4 29 23 25 12 1 2 4 0 1 3
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 0 0 0 0 – 0 1 3 7 4 0 0 0 0 1 0
Endosome 50 65 29 31 36 35 47 38 36 19 17 31 – 5 22 49 51 49 33 53 28 11 26 26
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 – 0 0 4 0 1 0 0 1 0 0 1
Mitochondria 16 30 1 9 1 34 42 58 89 58 139 89 – 0 2 25 13 14 5 5 2 1 2 4
Nucleus 35 60 43 28 47 60 65 106 55 70 56 63 – 7 34 89 51 53 20 42 21 5 17 11
Nuclear Periphery 0 2 0 0 1 1 2 5 0 0 1 4 – 0 0 4 1 0 0 1 1 0 0 1
Nucleolus 65 90 45 56 88 116 157 213 112 116 158 118 – 6 20 83 86 73 13 54 17 4 16 15
Peroxisomes 0 0 0 0 0 0 3 7 1 2 2 2 – 0 0 0 1 0 0 1 0 0 0 0
SpindlePole 58 41 77 64 115 93 144 254 87 94 72 90 – 4 27 53 65 56 112 136 113 23 44 29
Vac/Vac Membrane 18 35 15 8 7 5 5 10 7 3 2 7 – 1 5 18 14 17 8 13 11 1 5 11
Unique Cell Count 161 257 157 153 211 264 332 502 274 270 330 282 24 113 246 208 178 206 331 220 50 125 124
Labelled Cell Count 251 342 226 214 326 370 495 731 422 404 485 443 28 140 352 320 280 206 331 220 50 125 124


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.2 6.9 6.3 7.0 5.5 5.7 5.8 4.9 5.2 4.5 5.2 – 7.4 7.1 7.8 8.1 7.5 7.7 6.9 7.5
Std Deviation (1e-4) 0.7 1.0 1.3 1.8 2.0 1.3 1.6 1.1 1.3 1.4 1.3 2.5 – 1.2 1.8 1.7 1.8 1.8 1.7 1.5 2.4
Intensity Change (Log2) – – – -0.13 0.02 -0.31 -0.26 -0.25 -0.5 -0.4 -0.61 -0.39 – 0.11 0.04 0.19 0.24 0.13 0.16 0.01 0.12

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0.5 0.8 -0.3 -1.8 -1.9 -1.4 -1.3 -1.6 -1.6 – 0 3.7 0.1 0.9 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0.4 -0.4 -1.4 -1.2 -3.9 0 -3.6 0 -2.2 – 0 0.2 0.4 1.4 2.0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.4 4.4 4.2 0 6.0 0 7.7 – 0 0 3.8 2.8 3.2
Nucleus -1.9 -1.1 -1.1 -1.9 -1.6 -1.7 -0.3 -2.7 -1.2 – 0.2 0.5 1.8 -0.6 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 1.5 2.6 3.1 3.9 3.1 0 2.9 0 2.7 – -0.4 -2.1 1.1 2.5 2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole -1.3 1.0 -2.8 -1.2 0.3 0 -2.9 0 -3.5 – -3.0 -4.2 -5.8 -3.5 -3.3
Vacuole -1.5 -2.5 -3.6 -4.2 -4.3 -3.2 -4.2 -5.0 -3.3 – 0 -1.6 -0.8 -1.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.497 2.5182 2.2575 1.752 1.8132 2.2237 1.8232 2.3145 1.946 1.4835 2.0107 2.2267 1.844 1.8378 1.7708 1.0427 2.1039 1.0524
Actin 0.005 0.0022 0.0058 0.0002 0.0014 0.0005 0.017 0.0006 0.0129 0.0007 0.0527 0.0061 0.0359 0.0005 0.064 0.0783 0.0009 0.001
Bud 0.0005 0.0003 0.0005 0.0001 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0006 0.0001 0.001 0.0004 0.0014 0.001 0.0026 0.0006
Bud Neck 0.0034 0.0014 0.0058 0.0007 0.0023 0.0031 0.0042 0.0024 0.0059 0.0275 0.0061 0.0052 0.0057 0.002 0.0043 0.0042 0.0061 0.0091
Bud Periphery 0.0013 0.0002 0.0004 0 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0.0009 0.0001 0.0012 0.0002 0.0011 0.0011 0.0011 0.0005
Bud Site 0.0015 0.003 0.0027 0.0002 0.0007 0.0001 0.0013 0.0026 0.0033 0.0004 0.0026 0.0004 0.0156 0.0066 0.0331 0.0082 0.0057 0.0024
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0008 0.0001 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.0264 0.0859 0.016 0.0084 0.0235 0.0098 0.0425 0.0716 0.0197 0.0607 0.0162 0.0193 0.0363 0.1174 0.0123 0.065 0.2672 0.0455
Cytoplasmic Foci 0.0188 0.0068 0.004 0.0054 0.033 0.0022 0.0202 0.0112 0.0115 0.0069 0.0293 0.0031 0.0475 0.0168 0.008 0.0098 0.0274 0.0108
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.001 0 0.0004 0 0.0002 0.0003 0 0.0001
Endoplasmic Reticulum 0.0026 0.0024 0.0008 0.0002 0.0016 0.0003 0.0019 0.0022 0.0014 0.0016 0.0056 0.0009 0.0093 0.0037 0.0006 0.0025 0.0032 0.0008
Endosome 0.0246 0.009 0.0028 0.0008 0.0375 0.0021 0.0139 0.0053 0.0097 0.0055 0.0271 0.0029 0.0508 0.007 0.0035 0.0082 0.0339 0.0037
Golgi 0.004 0.0006 0.0009 0.0002 0.0029 0.0004 0.0033 0.0003 0.0022 0.0004 0.0083 0.0005 0.0249 0.0005 0.0042 0.014 0.0017 0.0007
Lipid Particles 0.0156 0.0008 0.0039 0.0009 0.0169 0.0006 0.0119 0.0009 0.0096 0.0008 0.0494 0.0005 0.0224 0.0006 0.0023 0.018 0.0016 0.0046
Mitochondria 0.0096 0.0008 0.0008 0.0002 0.0131 0.0084 0.0119 0.0006 0.0014 0.0004 0.0079 0.0005 0.0071 0.0005 0.0016 0.0055 0.002 0.0011
None 0.0125 0.019 0.0101 0.0009 0.0387 0.0256 0.0257 0.0241 0.0169 0.0072 0.0043 0.0247 0.01 0.0117 0.0012 0.0037 0.0209 0.0025
Nuclear Periphery 0.0421 0.0205 0.0091 0.0136 0.0423 0.013 0.0143 0.0175 0.0126 0.0217 0.0338 0.0107 0.0232 0.0239 0.0052 0.0093 0.0305 0.0117
Nucleolus 0.0263 0.0342 0.0389 0.0155 0.0361 0.0436 0.0159 0.0261 0.0238 0.0395 0.028 0.041 0.0071 0.0194 0.0302 0.0127 0.015 0.0321
Nucleus 0.3171 0.5044 0.3631 0.437 0.2389 0.3038 0.3529 0.4513 0.384 0.3629 0.2958 0.4258 0.3543 0.4418 0.3973 0.3011 0.3289 0.4548
Peroxisomes 0.0049 0.0022 0.0009 0.0058 0.0129 0.0003 0.0063 0.0004 0.0056 0.0006 0.01 0.0005 0.0262 0.0004 0.0068 0.0107 0.0012 0.0009
Punctate Nuclear 0.478 0.3018 0.5325 0.5096 0.4936 0.5855 0.4543 0.3803 0.4778 0.4608 0.4172 0.4567 0.308 0.3435 0.4214 0.4448 0.2367 0.4149
Vacuole 0.0027 0.0039 0.0007 0.0002 0.0029 0.0004 0.0013 0.0017 0.0007 0.0018 0.0017 0.0006 0.0102 0.0026 0.0008 0.0007 0.0112 0.0017
Vacuole Periphery 0.0024 0.0007 0.0002 0.0001 0.0013 0.0001 0.0004 0.0004 0.0004 0.0003 0.0014 0.0002 0.0023 0.0005 0.0002 0.0003 0.002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 7.0185 6.8105 9.3597 7.8304 1.8108 6.4141 8.474 7.9812 10.0179
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1935 1544 187 1089 1745 2263 1472 2998 3680 3807 1659 4087

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.68 728.83 1098.71 952.86 704.97 767.01 915.33 926.43 702.71 751.53 936.00 933.47
Standard Deviation 73.71 133.35 111.79 137.43 94.07 111.07 123.15 134.67 84.01 122.05 135.02 135.91
Intensity Change Log 2 — 0.056827 0.648983 0.443509 — 0.121684 0.376730 0.394120 — 0.089718 0.518855 0.418950

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000267 0.000760 0.002862 0.001406 0.000637 0.000302 0.001054 0.001599 0.000443 0.000488 0.001257 0.001548
Bud Neck 0.007093 0.012897 0.007596 0.009773 0.008133 0.015885 0.004299 0.032605 0.007586 0.014673 0.004671 0.026521
Bud Site 0.002441 0.007463 0.005611 0.004599 0.005076 0.007295 0.002249 0.014555 0.003690 0.007363 0.002628 0.011902
Cell Periphery 0.000163 0.000220 0.000180 0.000080 0.000216 0.000132 0.000183 0.000149 0.000188 0.000167 0.000183 0.000130
Cytoplasm 0.132536 0.151934 0.007157 0.009214 0.129122 0.159945 0.047989 0.116047 0.130917 0.156696 0.043386 0.087580
Cytoplasmic Foci 0.064406 0.095425 0.001210 0.001286 0.055749 0.033168 0.004155 0.003232 0.060301 0.058418 0.003823 0.002713
Eisosomes 0.000074 0.000089 0.000100 0.000021 0.000090 0.000050 0.000057 0.000030 0.000081 0.000066 0.000062 0.000027
Endoplasmic Reticulum 0.000786 0.002779 0.012683 0.004211 0.001883 0.001433 0.003855 0.001866 0.001306 0.001979 0.004850 0.002491
Endosome 0.005009 0.012885 0.002361 0.002535 0.004854 0.010446 0.001673 0.004169 0.004935 0.011435 0.001750 0.003733
Golgi 0.003296 0.006085 0.000794 0.001947 0.003331 0.002576 0.000700 0.001100 0.003313 0.003999 0.000710 0.001326
Lipid Particles 0.008970 0.020781 0.002815 0.000767 0.010223 0.005863 0.002113 0.000829 0.009564 0.011913 0.002192 0.000813
Mitochondria 0.005817 0.013137 0.001042 0.003255 0.005598 0.012487 0.001653 0.005965 0.005713 0.012751 0.001584 0.005243
Mitotic Spindle 0.055299 0.238715 0.528565 0.799297 0.099667 0.224330 0.335439 0.319199 0.076338 0.230164 0.357208 0.447124
None 0.062870 0.056125 0.001953 0.002886 0.059441 0.027092 0.017171 0.020734 0.061244 0.038867 0.015455 0.015978
Nuclear Periphery 0.000889 0.002852 0.001768 0.003413 0.001665 0.002912 0.001663 0.002706 0.001257 0.002888 0.001675 0.002894
Nuclear Periphery Foci 0.014246 0.038360 0.025035 0.010134 0.042861 0.010678 0.009024 0.001765 0.027815 0.021905 0.010829 0.003995
Nucleolus 0.338021 0.117866 0.030262 0.022318 0.211928 0.116685 0.056758 0.035434 0.278229 0.117164 0.053771 0.031939
Nucleus 0.287333 0.198741 0.337637 0.112839 0.347516 0.352280 0.498655 0.422322 0.315871 0.290010 0.480505 0.339859
Peroxisomes 0.008833 0.012353 0.002234 0.000937 0.008963 0.005062 0.002373 0.003310 0.008895 0.008019 0.002357 0.002678
Vacuole 0.001243 0.009118 0.027793 0.006929 0.002623 0.009773 0.008703 0.010939 0.001897 0.009507 0.010854 0.009871
Vacuole Periphery 0.000409 0.001416 0.000343 0.002153 0.000425 0.001606 0.000236 0.001446 0.000416 0.001529 0.000248 0.001635

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.75 -11.76 -9.91 -6.62 5.86 3.34 -17.13 -3.49 -4.41 5.27 -5.12 -22.36 -8.09 -6.39 6.08
Bud Neck -8.66 -10.32 -17.16 -12.27 -1.47 -8.06 -10.53 -24.32 -17.15 -17.18 -11.84 -15.59 -29.83 -21.31 -17.42
Bud Site -8.42 -10.69 -10.43 -4.94 4.59 -1.53 -5.39 -7.21 -6.06 -3.41 -6.13 -12.53 -12.39 -7.50 -2.24
Cell Periphery -7.25 -8.77 -5.21 2.40 6.76 4.56 -0.73 4.89 -0.52 6.64 -1.10 -6.35 1.24 2.37 7.82
Cytoplasm -7.32 13.08 13.00 17.83 -1.19 -7.83 9.19 -0.52 8.20 -12.05 -10.97 11.74 2.70 13.94 -10.11
Cytoplasmic Foci -1.70 19.91 20.20 21.74 2.88 8.47 19.83 20.09 17.15 2.22 5.14 27.80 28.21 27.05 2.92
Eisosomes -3.26 -5.86 11.72 12.25 8.68 11.70 3.65 22.68 12.07 14.62 6.96 -0.75 27.66 18.47 16.15
Endoplasmic Reticulum -11.89 -11.10 -12.13 -8.38 6.58 3.07 -17.99 -8.17 -10.68 14.01 -7.57 -22.24 -16.56 -11.81 12.37
Endosome -8.15 -0.88 4.96 11.88 4.90 -3.79 11.26 10.77 9.09 1.60 -8.09 8.62 10.12 14.25 3.06
Golgi -1.38 7.66 10.40 11.16 3.78 7.34 14.37 15.24 6.65 3.14 4.40 17.59 18.58 13.31 3.58
Lipid Particles -6.43 4.32 9.03 12.45 5.52 4.63 11.89 13.58 11.97 6.84 -2.38 13.22 15.94 16.53 8.01
Mitochondria -5.63 3.25 -1.65 4.69 -5.35 -4.33 5.39 -2.60 3.30 -10.11 -6.78 5.44 -3.59 5.17 -11.26
Mitotic Spindle -18.03 -11.35 -35.07 -18.18 -5.00 -11.36 -15.76 -15.74 -3.54 3.27 -21.05 -21.75 -32.84 -11.35 -1.91
None 2.54 22.90 21.00 12.71 -6.88 7.77 13.96 11.18 3.92 -5.52 7.84 21.40 19.84 10.71 -4.42
Nuclear Periphery -4.82 -8.22 -13.85 -7.54 -3.92 -6.36 -9.37 -10.95 -4.23 -4.52 -8.41 -15.04 -17.92 -7.67 -7.08
Nuclear Periphery Foci -5.62 0.17 7.68 11.54 3.98 12.87 14.90 20.54 13.95 10.86 6.00 14.53 23.58 18.85 9.37
Nucleolus 29.80 32.60 44.93 13.04 0.62 11.80 22.81 29.76 18.04 4.69 29.01 38.73 52.24 21.51 5.43
Nucleus -0.90 -7.04 -9.52 -8.53 2.76 -7.35 -19.30 -27.94 -20.12 -2.58 -7.95 -23.86 -32.73 -24.31 1.07
Peroxisomes -3.46 4.87 12.20 15.18 7.50 6.72 11.23 12.93 10.76 2.75 2.75 13.44 17.26 17.85 4.19
Vacuole -7.02 -6.30 -8.29 -3.27 4.59 -6.65 -9.53 -10.43 -2.97 1.79 -9.77 -11.57 -13.68 -4.29 3.81
Vacuole Periphery -6.74 -10.45 -2.48 -0.35 -0.42 -3.99 -3.74 -3.55 1.24 -2.87 -6.23 -8.41 -4.43 0.90 -2.99
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
Localization
Cell Percentages spindle pole (16%), nucleolus (9%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cep3

Cep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available