Standard name | |
---|---|
Human Ortholog | |
Description | Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 2 | 3 | 0 | 0 | 1 | 1 | 4 | 3 | 7 | 10 | 6 | 11 | – | 1 | 0 | 0 | 5 | 0 | 1 | 2 | 3 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 2 | 3 | 7 | 2 | 3 | – | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 1 | 1 |
Bud Site | 0 | 1 | 0 | 1 | 1 | 4 | 8 | 11 | 10 | 9 | 13 | 9 | – | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 15 | 14 | 16 | 24 | 21 | 16 | 24 | 15 | 15 | 17 | 14 | – | 4 | 29 | 23 | 25 | 12 | 1 | 2 | 4 | 0 | 1 | 3 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 3 | 7 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 50 | 65 | 29 | 31 | 36 | 35 | 47 | 38 | 36 | 19 | 17 | 31 | – | 5 | 22 | 49 | 51 | 49 | 33 | 53 | 28 | 11 | 26 | 26 |
Golgi | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Mitochondria | 16 | 30 | 1 | 9 | 1 | 34 | 42 | 58 | 89 | 58 | 139 | 89 | – | 0 | 2 | 25 | 13 | 14 | 5 | 5 | 2 | 1 | 2 | 4 |
Nucleus | 35 | 60 | 43 | 28 | 47 | 60 | 65 | 106 | 55 | 70 | 56 | 63 | – | 7 | 34 | 89 | 51 | 53 | 20 | 42 | 21 | 5 | 17 | 11 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 5 | 0 | 0 | 1 | 4 | – | 0 | 0 | 4 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Nucleolus | 65 | 90 | 45 | 56 | 88 | 116 | 157 | 213 | 112 | 116 | 158 | 118 | – | 6 | 20 | 83 | 86 | 73 | 13 | 54 | 17 | 4 | 16 | 15 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 1 | 2 | 2 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 58 | 41 | 77 | 64 | 115 | 93 | 144 | 254 | 87 | 94 | 72 | 90 | – | 4 | 27 | 53 | 65 | 56 | 112 | 136 | 113 | 23 | 44 | 29 |
Vac/Vac Membrane | 18 | 35 | 15 | 8 | 7 | 5 | 5 | 10 | 7 | 3 | 2 | 7 | – | 1 | 5 | 18 | 14 | 17 | 8 | 13 | 11 | 1 | 5 | 11 |
Unique Cell Count | 161 | 257 | 157 | 153 | 211 | 264 | 332 | 502 | 274 | 270 | 330 | 282 | 24 | 113 | 246 | 208 | 178 | 206 | 331 | 220 | 50 | 125 | 124 | |
Labelled Cell Count | 251 | 342 | 226 | 214 | 326 | 370 | 495 | 731 | 422 | 404 | 485 | 443 | 28 | 140 | 352 | 320 | 280 | 206 | 331 | 220 | 50 | 125 | 124 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.2 | 6.9 | 6.3 | 7.0 | 5.5 | 5.7 | 5.8 | 4.9 | 5.2 | 4.5 | 5.2 | – | 7.4 | 7.1 | 7.8 | 8.1 | 7.5 | 7.7 | 6.9 | 7.5 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.3 | 1.8 | 2.0 | 1.3 | 1.6 | 1.1 | 1.3 | 1.4 | 1.3 | 2.5 | – | 1.2 | 1.8 | 1.7 | 1.8 | 1.8 | 1.7 | 1.5 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.02 | -0.31 | -0.26 | -0.25 | -0.5 | -0.4 | -0.61 | -0.39 | – | 0.11 | 0.04 | 0.19 | 0.24 | 0.13 | 0.16 | 0.01 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0.8 | -0.3 | -1.8 | -1.9 | -1.4 | -1.3 | -1.6 | -1.6 | – | 0 | 3.7 | 0.1 | 0.9 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.4 | -0.4 | -1.4 | -1.2 | -3.9 | 0 | -3.6 | 0 | -2.2 | – | 0 | 0.2 | 0.4 | 1.4 | 2.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.4 | 4.4 | 4.2 | 0 | 6.0 | 0 | 7.7 | – | 0 | 0 | 3.8 | 2.8 | 3.2 |
Nucleus | -1.9 | -1.1 | -1.1 | -1.9 | -1.6 | -1.7 | -0.3 | -2.7 | -1.2 | – | 0.2 | 0.5 | 1.8 | -0.6 | 0.5 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.5 | 2.6 | 3.1 | 3.9 | 3.1 | 0 | 2.9 | 0 | 2.7 | – | -0.4 | -2.1 | 1.1 | 2.5 | 2.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -1.3 | 1.0 | -2.8 | -1.2 | 0.3 | 0 | -2.9 | 0 | -3.5 | – | -3.0 | -4.2 | -5.8 | -3.5 | -3.3 |
Vacuole | -1.5 | -2.5 | -3.6 | -4.2 | -4.3 | -3.2 | -4.2 | -5.0 | -3.3 | – | 0 | -1.6 | -0.8 | -1.0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.497 | 2.5182 | 2.2575 | 1.752 | 1.8132 | 2.2237 | 1.8232 | 2.3145 | 1.946 | 1.4835 | 2.0107 | 2.2267 | 1.844 | 1.8378 | 1.7708 | 1.0427 | 2.1039 | 1.0524 |
Actin | 0.005 | 0.0022 | 0.0058 | 0.0002 | 0.0014 | 0.0005 | 0.017 | 0.0006 | 0.0129 | 0.0007 | 0.0527 | 0.0061 | 0.0359 | 0.0005 | 0.064 | 0.0783 | 0.0009 | 0.001 |
Bud | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.001 | 0.0004 | 0.0014 | 0.001 | 0.0026 | 0.0006 |
Bud Neck | 0.0034 | 0.0014 | 0.0058 | 0.0007 | 0.0023 | 0.0031 | 0.0042 | 0.0024 | 0.0059 | 0.0275 | 0.0061 | 0.0052 | 0.0057 | 0.002 | 0.0043 | 0.0042 | 0.0061 | 0.0091 |
Bud Periphery | 0.0013 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0012 | 0.0002 | 0.0011 | 0.0011 | 0.0011 | 0.0005 |
Bud Site | 0.0015 | 0.003 | 0.0027 | 0.0002 | 0.0007 | 0.0001 | 0.0013 | 0.0026 | 0.0033 | 0.0004 | 0.0026 | 0.0004 | 0.0156 | 0.0066 | 0.0331 | 0.0082 | 0.0057 | 0.0024 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.0264 | 0.0859 | 0.016 | 0.0084 | 0.0235 | 0.0098 | 0.0425 | 0.0716 | 0.0197 | 0.0607 | 0.0162 | 0.0193 | 0.0363 | 0.1174 | 0.0123 | 0.065 | 0.2672 | 0.0455 |
Cytoplasmic Foci | 0.0188 | 0.0068 | 0.004 | 0.0054 | 0.033 | 0.0022 | 0.0202 | 0.0112 | 0.0115 | 0.0069 | 0.0293 | 0.0031 | 0.0475 | 0.0168 | 0.008 | 0.0098 | 0.0274 | 0.0108 |
Eisosomes | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.001 | 0 | 0.0004 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0026 | 0.0024 | 0.0008 | 0.0002 | 0.0016 | 0.0003 | 0.0019 | 0.0022 | 0.0014 | 0.0016 | 0.0056 | 0.0009 | 0.0093 | 0.0037 | 0.0006 | 0.0025 | 0.0032 | 0.0008 |
Endosome | 0.0246 | 0.009 | 0.0028 | 0.0008 | 0.0375 | 0.0021 | 0.0139 | 0.0053 | 0.0097 | 0.0055 | 0.0271 | 0.0029 | 0.0508 | 0.007 | 0.0035 | 0.0082 | 0.0339 | 0.0037 |
Golgi | 0.004 | 0.0006 | 0.0009 | 0.0002 | 0.0029 | 0.0004 | 0.0033 | 0.0003 | 0.0022 | 0.0004 | 0.0083 | 0.0005 | 0.0249 | 0.0005 | 0.0042 | 0.014 | 0.0017 | 0.0007 |
Lipid Particles | 0.0156 | 0.0008 | 0.0039 | 0.0009 | 0.0169 | 0.0006 | 0.0119 | 0.0009 | 0.0096 | 0.0008 | 0.0494 | 0.0005 | 0.0224 | 0.0006 | 0.0023 | 0.018 | 0.0016 | 0.0046 |
Mitochondria | 0.0096 | 0.0008 | 0.0008 | 0.0002 | 0.0131 | 0.0084 | 0.0119 | 0.0006 | 0.0014 | 0.0004 | 0.0079 | 0.0005 | 0.0071 | 0.0005 | 0.0016 | 0.0055 | 0.002 | 0.0011 |
None | 0.0125 | 0.019 | 0.0101 | 0.0009 | 0.0387 | 0.0256 | 0.0257 | 0.0241 | 0.0169 | 0.0072 | 0.0043 | 0.0247 | 0.01 | 0.0117 | 0.0012 | 0.0037 | 0.0209 | 0.0025 |
Nuclear Periphery | 0.0421 | 0.0205 | 0.0091 | 0.0136 | 0.0423 | 0.013 | 0.0143 | 0.0175 | 0.0126 | 0.0217 | 0.0338 | 0.0107 | 0.0232 | 0.0239 | 0.0052 | 0.0093 | 0.0305 | 0.0117 |
Nucleolus | 0.0263 | 0.0342 | 0.0389 | 0.0155 | 0.0361 | 0.0436 | 0.0159 | 0.0261 | 0.0238 | 0.0395 | 0.028 | 0.041 | 0.0071 | 0.0194 | 0.0302 | 0.0127 | 0.015 | 0.0321 |
Nucleus | 0.3171 | 0.5044 | 0.3631 | 0.437 | 0.2389 | 0.3038 | 0.3529 | 0.4513 | 0.384 | 0.3629 | 0.2958 | 0.4258 | 0.3543 | 0.4418 | 0.3973 | 0.3011 | 0.3289 | 0.4548 |
Peroxisomes | 0.0049 | 0.0022 | 0.0009 | 0.0058 | 0.0129 | 0.0003 | 0.0063 | 0.0004 | 0.0056 | 0.0006 | 0.01 | 0.0005 | 0.0262 | 0.0004 | 0.0068 | 0.0107 | 0.0012 | 0.0009 |
Punctate Nuclear | 0.478 | 0.3018 | 0.5325 | 0.5096 | 0.4936 | 0.5855 | 0.4543 | 0.3803 | 0.4778 | 0.4608 | 0.4172 | 0.4567 | 0.308 | 0.3435 | 0.4214 | 0.4448 | 0.2367 | 0.4149 |
Vacuole | 0.0027 | 0.0039 | 0.0007 | 0.0002 | 0.0029 | 0.0004 | 0.0013 | 0.0017 | 0.0007 | 0.0018 | 0.0017 | 0.0006 | 0.0102 | 0.0026 | 0.0008 | 0.0007 | 0.0112 | 0.0017 |
Vacuole Periphery | 0.0024 | 0.0007 | 0.0002 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0.0004 | 0.0004 | 0.0003 | 0.0014 | 0.0002 | 0.0023 | 0.0005 | 0.0002 | 0.0003 | 0.002 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.3414 | 7.0185 | 6.8105 | 9.3597 | 7.8304 | 1.8108 | 6.4141 | 8.474 | 7.9812 | 10.0179 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1935 | 1544 | 187 | 1089 | 1745 | 2263 | 1472 | 2998 | 3680 | 3807 | 1659 | 4087 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 700.68 | 728.83 | 1098.71 | 952.86 | 704.97 | 767.01 | 915.33 | 926.43 | 702.71 | 751.53 | 936.00 | 933.47 |
Standard Deviation | 73.71 | 133.35 | 111.79 | 137.43 | 94.07 | 111.07 | 123.15 | 134.67 | 84.01 | 122.05 | 135.02 | 135.91 |
Intensity Change Log 2 | — | 0.056827 | 0.648983 | 0.443509 | — | 0.121684 | 0.376730 | 0.394120 | — | 0.089718 | 0.518855 | 0.418950 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000267 | 0.000760 | 0.002862 | 0.001406 | 0.000637 | 0.000302 | 0.001054 | 0.001599 | 0.000443 | 0.000488 | 0.001257 | 0.001548 |
Bud Neck | 0.007093 | 0.012897 | 0.007596 | 0.009773 | 0.008133 | 0.015885 | 0.004299 | 0.032605 | 0.007586 | 0.014673 | 0.004671 | 0.026521 |
Bud Site | 0.002441 | 0.007463 | 0.005611 | 0.004599 | 0.005076 | 0.007295 | 0.002249 | 0.014555 | 0.003690 | 0.007363 | 0.002628 | 0.011902 |
Cell Periphery | 0.000163 | 0.000220 | 0.000180 | 0.000080 | 0.000216 | 0.000132 | 0.000183 | 0.000149 | 0.000188 | 0.000167 | 0.000183 | 0.000130 |
Cytoplasm | 0.132536* | 0.151934* | 0.007157 | 0.009214 | 0.129122* | 0.159945* | 0.047989 | 0.116047* | 0.130917* | 0.156696* | 0.043386 | 0.087580 |
Cytoplasmic Foci | 0.064406 | 0.095425 | 0.001210 | 0.001286 | 0.055749 | 0.033168 | 0.004155 | 0.003232 | 0.060301 | 0.058418 | 0.003823 | 0.002713 |
Eisosomes | 0.000074 | 0.000089 | 0.000100 | 0.000021 | 0.000090 | 0.000050 | 0.000057 | 0.000030 | 0.000081 | 0.000066 | 0.000062 | 0.000027 |
Endoplasmic Reticulum | 0.000786 | 0.002779 | 0.012683 | 0.004211 | 0.001883 | 0.001433 | 0.003855 | 0.001866 | 0.001306 | 0.001979 | 0.004850 | 0.002491 |
Endosome | 0.005009 | 0.012885 | 0.002361 | 0.002535 | 0.004854 | 0.010446 | 0.001673 | 0.004169 | 0.004935 | 0.011435 | 0.001750 | 0.003733 |
Golgi | 0.003296 | 0.006085 | 0.000794 | 0.001947 | 0.003331 | 0.002576 | 0.000700 | 0.001100 | 0.003313 | 0.003999 | 0.000710 | 0.001326 |
Lipid Particles | 0.008970 | 0.020781 | 0.002815 | 0.000767 | 0.010223 | 0.005863 | 0.002113 | 0.000829 | 0.009564 | 0.011913 | 0.002192 | 0.000813 |
Mitochondria | 0.005817 | 0.013137 | 0.001042 | 0.003255 | 0.005598 | 0.012487 | 0.001653 | 0.005965 | 0.005713 | 0.012751 | 0.001584 | 0.005243 |
Mitotic Spindle | 0.055299 | 0.238715* | 0.528565* | 0.799297* | 0.099667 | 0.224330* | 0.335439* | 0.319199* | 0.076338 | 0.230164* | 0.357208* | 0.447124* |
None | 0.062870 | 0.056125 | 0.001953 | 0.002886 | 0.059441 | 0.027092 | 0.017171 | 0.020734 | 0.061244 | 0.038867 | 0.015455 | 0.015978 |
Nuclear Periphery | 0.000889 | 0.002852 | 0.001768 | 0.003413 | 0.001665 | 0.002912 | 0.001663 | 0.002706 | 0.001257 | 0.002888 | 0.001675 | 0.002894 |
Nuclear Periphery Foci | 0.014246 | 0.038360 | 0.025035 | 0.010134 | 0.042861 | 0.010678 | 0.009024 | 0.001765 | 0.027815 | 0.021905 | 0.010829 | 0.003995 |
Nucleolus | 0.338021* | 0.117866 | 0.030262 | 0.022318 | 0.211928 | 0.116685 | 0.056758 | 0.035434 | 0.278229* | 0.117164 | 0.053771 | 0.031939 |
Nucleus | 0.287333* | 0.198741* | 0.337637* | 0.112839 | 0.347516* | 0.352280* | 0.498655* | 0.422322* | 0.315871* | 0.290010* | 0.480505* | 0.339859* |
Peroxisomes | 0.008833 | 0.012353 | 0.002234 | 0.000937 | 0.008963 | 0.005062 | 0.002373 | 0.003310 | 0.008895 | 0.008019 | 0.002357 | 0.002678 |
Vacuole | 0.001243 | 0.009118 | 0.027793 | 0.006929 | 0.002623 | 0.009773 | 0.008703 | 0.010939 | 0.001897 | 0.009507 | 0.010854 | 0.009871 |
Vacuole Periphery | 0.000409 | 0.001416 | 0.000343 | 0.002153 | 0.000425 | 0.001606 | 0.000236 | 0.001446 | 0.000416 | 0.001529 | 0.000248 | 0.001635 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.75 | -11.76 | -9.91 | -6.62 | 5.86 | 3.34 | -17.13 | -3.49 | -4.41 | 5.27 | -5.12 | -22.36 | -8.09 | -6.39 | 6.08 |
Bud Neck | -8.66 | -10.32 | -17.16 | -12.27 | -1.47 | -8.06 | -10.53 | -24.32 | -17.15 | -17.18 | -11.84 | -15.59 | -29.83 | -21.31 | -17.42 |
Bud Site | -8.42 | -10.69 | -10.43 | -4.94 | 4.59 | -1.53 | -5.39 | -7.21 | -6.06 | -3.41 | -6.13 | -12.53 | -12.39 | -7.50 | -2.24 |
Cell Periphery | -7.25 | -8.77 | -5.21 | 2.40 | 6.76 | 4.56 | -0.73 | 4.89 | -0.52 | 6.64 | -1.10 | -6.35 | 1.24 | 2.37 | 7.82 |
Cytoplasm | -7.32 | 13.08 | 13.00 | 17.83 | -1.19 | -7.83 | 9.19 | -0.52 | 8.20 | -12.05 | -10.97 | 11.74 | 2.70 | 13.94 | -10.11 |
Cytoplasmic Foci | -1.70 | 19.91 | 20.20 | 21.74 | 2.88 | 8.47 | 19.83 | 20.09 | 17.15 | 2.22 | 5.14 | 27.80 | 28.21 | 27.05 | 2.92 |
Eisosomes | -3.26 | -5.86 | 11.72 | 12.25 | 8.68 | 11.70 | 3.65 | 22.68 | 12.07 | 14.62 | 6.96 | -0.75 | 27.66 | 18.47 | 16.15 |
Endoplasmic Reticulum | -11.89 | -11.10 | -12.13 | -8.38 | 6.58 | 3.07 | -17.99 | -8.17 | -10.68 | 14.01 | -7.57 | -22.24 | -16.56 | -11.81 | 12.37 |
Endosome | -8.15 | -0.88 | 4.96 | 11.88 | 4.90 | -3.79 | 11.26 | 10.77 | 9.09 | 1.60 | -8.09 | 8.62 | 10.12 | 14.25 | 3.06 |
Golgi | -1.38 | 7.66 | 10.40 | 11.16 | 3.78 | 7.34 | 14.37 | 15.24 | 6.65 | 3.14 | 4.40 | 17.59 | 18.58 | 13.31 | 3.58 |
Lipid Particles | -6.43 | 4.32 | 9.03 | 12.45 | 5.52 | 4.63 | 11.89 | 13.58 | 11.97 | 6.84 | -2.38 | 13.22 | 15.94 | 16.53 | 8.01 |
Mitochondria | -5.63 | 3.25 | -1.65 | 4.69 | -5.35 | -4.33 | 5.39 | -2.60 | 3.30 | -10.11 | -6.78 | 5.44 | -3.59 | 5.17 | -11.26 |
Mitotic Spindle | -18.03* | -11.35 | -35.07* | -18.18 | -5.00 | -11.36 | -15.76 | -15.74 | -3.54 | 3.27 | -21.05 | -21.75 | -32.84 | -11.35 | -1.91 |
None | 2.54 | 22.90 | 21.00 | 12.71 | -6.88 | 7.77 | 13.96 | 11.18 | 3.92 | -5.52 | 7.84 | 21.40 | 19.84 | 10.71 | -4.42 |
Nuclear Periphery | -4.82 | -8.22 | -13.85 | -7.54 | -3.92 | -6.36 | -9.37 | -10.95 | -4.23 | -4.52 | -8.41 | -15.04 | -17.92 | -7.67 | -7.08 |
Nuclear Periphery Foci | -5.62 | 0.17 | 7.68 | 11.54 | 3.98 | 12.87 | 14.90 | 20.54* | 13.95 | 10.86 | 6.00 | 14.53 | 23.58 | 18.85 | 9.37 |
Nucleolus | 29.80* | 32.60* | 44.93* | 13.04 | 0.62 | 11.80 | 22.81* | 29.76* | 18.04 | 4.69 | 29.01* | 38.73* | 52.24* | 21.51 | 5.43 |
Nucleus | -0.90 | -7.04 | -9.52 | -8.53 | 2.76 | -7.35 | -19.30 | -27.94 | -20.12 | -2.58 | -7.95 | -23.86 | -32.73 | -24.31 | 1.07 |
Peroxisomes | -3.46 | 4.87 | 12.20 | 15.18 | 7.50 | 6.72 | 11.23 | 12.93 | 10.76 | 2.75 | 2.75 | 13.44 | 17.26 | 17.85 | 4.19 |
Vacuole | -7.02 | -6.30 | -8.29 | -3.27 | 4.59 | -6.65 | -9.53 | -10.43 | -2.97 | 1.79 | -9.77 | -11.57 | -13.68 | -4.29 | 3.81 |
Vacuole Periphery | -6.74 | -10.45 | -2.48 | -0.35 | -0.42 | -3.99 | -3.74 | -3.55 | 1.24 | -2.87 | -6.23 | -8.41 | -4.43 | 0.90 | -2.99 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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