Standard name
Human Ortholog
Description Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.91 0.94 0.98 0.97 0.93 0.89 0.77 0.84 0.71 0.73 0.58 0.62 0.99 0.99 1.0 – – 0.91 0.83 0.85 0.79 0.86 0.8 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0.17 0.09 0.05 0.12 0.09 0.27 0.47 0.34 0.5 0.44 0.58 0.58 0 0 0 – – 0.05 0.09 0.06 0.08 0 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 3 1 4 2 5 2
Bud 2 2 2 1 2 2 0 3 8 2 5 7 0 0 0 – – 0 1 1 0 4 8 8
Bud Neck 0 0 0 0 1 1 0 2 2 1 0 0 0 0 0 – – 0 1 0 0 0 3 3
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 – – 0 – – – – – –
Cell Periphery 0 2 2 0 1 1 1 0 1 2 2 5 0 0 1 – – 4 1 0 2 0 2 3
Cytoplasm 261 221 170 138 199 181 176 233 165 175 113 138 199 343 309 – – 118 241 206 136 175 318 192
Endoplasmic Reticulum 1 2 0 0 0 1 1 0 0 1 0 0 0 1 1 – – 8 1 0 3 2 2 0
Endosome 0 0 0 0 2 0 0 0 1 0 0 0 0 1 0 – – 3 2 2 1 0 7 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 – – 0 0 2 1 0 2 1
Mitochondria 50 22 9 17 19 55 107 95 115 105 114 129 3 3 2 – – 7 27 14 14 7 29 18
Nucleus 0 0 0 1 2 0 0 1 0 1 3 2 0 0 2 – – 1 0 0 0 1 0 0
Nuclear Periphery 0 1 0 0 1 0 0 1 1 2 3 3 0 0 0 – – 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 – – 0 1 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 4 4 1 2 1 0 0 0 – – 0 1 2 1 2 5 2
Vac/Vac Membrane 0 1 0 0 0 0 3 7 2 3 5 3 0 1 0 – – 5 4 2 2 1 5 11
Unique Cell Count 288 235 174 143 215 203 229 276 232 241 195 223 200 347 310 129 292 242 173 203 399 253
Labelled Cell Count 315 251 183 157 228 241 289 346 300 293 247 289 203 350 315 146 292 242 173 203 399 253


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.4 3.8 3.4 3.4 2.9 2.4 3.0 2.8 2.6 2.7 2.5 3.8 3.9 4.4 4.2 4.8 5.2 3.8 3.9 4.7
Std Deviation (1e-4) 0.5 0.7 1.1 1.1 1.1 1.0 0.7 1.1 1.7 1.1 1.1 1.5 1.1 1.2 2.0 1.9 1.5 1.5 1.4 1.3 1.8
Intensity Change (Log2) – – – -0.16 -0.14 -0.39 -0.68 -0.35 -0.44 -0.51 -0.5 -0.59 -0.01 0.06 0.21 0.17 0.36 0.47 -0.0 0.03 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0687 0.748 1.5124 1.7354 1.1645 1.7449 0.197 0.621 0.607 0.6334 0.631 0.4643 1.021 1.4534 0.9667 1.641 1.9807 0.7212
Actin 0.0419 0.0001 0.0003 0.0002 0.0002 0.0004 0.0468 0.0069 0.0123 0.0382 0.0126 0.009 0.1084 0.0003 0.0179 0.041 0.1829 0.0119
Bud 0.014 0 0.0001 0.0001 0 0.0001 0.0018 0.0046 0.0023 0.0003 0.0012 0.0007 0.0015 0.0029 0.0146 0.0002 0.0017 0.0002
Bud Neck 0.0008 0 0.0001 0.0001 0.0002 0.0003 0.0039 0.0001 0.0005 0.0001 0.0015 0.0006 0.0036 0.0002 0.0002 0.001 0.0395 0.0003
Bud Periphery 0.0283 0 0 0.0001 0 0.0001 0.0042 0.0178 0.0029 0.0003 0.0028 0.0015 0.003 0.0019 0.0059 0.0003 0.0025 0.0004
Bud Site 0.0075 0 0.0001 0 0.0001 0 0.0043 0.0009 0.0112 0.0002 0.0069 0.0002 0.005 0.0075 0.0027 0.0014 0.0184 0.0001
Cell Periphery 0.0006 0.0001 0 0 0.0001 0.0001 0.0006 0.0002 0.0003 0.0001 0.0007 0.0001 0.0002 0.0005 0.0002 0.0003 0.0007 0
Cytoplasm 0.0088 0.2186 0.1799 0.2411 0.4413 0.2471 0.0849 0.28 0.1342 0.1803 0.244 0.1374 0.1145 0.23 0.1614 0.309 0.0662 0.2092
Cytoplasmic Foci 0.0084 0.0006 0.0046 0.0027 0.0021 0.0202 0.0286 0.0037 0.0168 0.0118 0.0176 0.0088 0.0242 0.0052 0.015 0.0135 0.0462 0.0139
Eisosomes 0.0008 0.0002 0.0001 0.0001 0 0.0001 0.001 0.0002 0.0004 0.0003 0.0003 0.0004 0.0005 0.0002 0.0004 0.0005 0.0009 0.0001
Endoplasmic Reticulum 0.0483 0.0004 0.0004 0.0008 0.0025 0.0017 0.0049 0.0012 0.0036 0.0011 0.0033 0.0006 0.0011 0.0006 0.0018 0.0016 0.0012 0.0006
Endosome 0.0268 0 0.0012 0.0005 0.0007 0.0009 0.0105 0.0006 0.0033 0.0049 0.0148 0.0006 0.0061 0.0001 0.004 0.0134 0.0168 0.0011
Golgi 0.0077 0 0.0019 0.0001 0.0001 0.0001 0.0049 0.0003 0.0013 0.0014 0.009 0.0001 0.0053 0 0.0029 0.0065 0.0376 0.0013
Lipid Particles 0.0029 0 0.0109 0.0002 0.0001 0.0009 0.0059 0.0004 0.0037 0.0006 0.016 0.0001 0.0017 0.0001 0.0061 0.0255 0.105 0.0004
Mitochondria 0.0147 0 0.0033 0.0001 0.0001 0.0001 0.0027 0.0007 0.0024 0.0004 0.0153 0.0002 0.003 0.0001 0.0155 0.0062 0.0237 0.0026
None 0.7156 0.7793 0.7636 0.7521 0.5511 0.7251 0.765 0.6801 0.7779 0.7521 0.6245 0.8363 0.7053 0.7487 0.7375 0.5685 0.3078 0.7544
Nuclear Periphery 0.052 0.0001 0.0004 0.0003 0.0003 0.0004 0.0078 0.0004 0.0061 0.0005 0.0007 0.0004 0.0004 0.0002 0.0008 0.0004 0.0005 0.0002
Nucleolus 0.0005 0 0.0001 0 0 0.0001 0.0006 0.0001 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0004 0.0007 0.0007
Nucleus 0.0033 0.0002 0.0061 0.0007 0.0006 0.0004 0.0023 0.0007 0.0011 0.0007 0.0009 0.0007 0.0005 0.0007 0.0007 0.0006 0.0007 0.0005
Peroxisomes 0.0065 0 0.0261 0.0001 0 0.0008 0.0138 0.0003 0.0166 0.0034 0.023 0.0002 0.0124 0.0002 0.0099 0.007 0.14 0.0008
Punctate Nuclear 0.0011 0.0001 0.0002 0.0002 0.0002 0.0007 0.0032 0.0004 0.0014 0.0005 0.0009 0.0016 0.0014 0.0003 0.0012 0.0007 0.0044 0.0011
Vacuole 0.0048 0 0.0005 0.0003 0.0003 0.0005 0.0015 0.0004 0.0007 0.0023 0.0028 0.0003 0.0012 0.0002 0.0008 0.0017 0.0017 0.0001
Vacuole Periphery 0.0047 0 0.0001 0 0 0.0001 0.0011 0.0001 0.0003 0.0004 0.0009 0.0001 0.0003 0 0.0002 0.0003 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.0681 11.0246 10.1056 8.9616 6.584 10.8757 8.6881 9.0724 12.9366 11.1463
Translational Efficiency 0.6896 0.4821 0.4746 0.6075 0.8358 0.6438 0.6961 0.7763 0.514 0.63

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2477 1158 565 1675 1167 177 309 1090 3644 1335 874 2765

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 644.89 665.54 885.73 804.44 653.16 708.16 945.59 838.73 647.54 671.19 906.89 817.96
Standard Deviation 64.78 93.87 186.27 251.66 71.74 92.40 143.88 321.34 67.20 94.78 174.84 281.70
Intensity Change Log 2 — 0.045472 0.457814 0.318932 — 0.116639 0.533778 0.360770 — 0.081721 0.496538 0.340136

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000060 0.000463 0.000330 0.001497 0.000051 0.000417 0.000138 0.000899 0.000057 0.000457 0.000262 0.001261
Bud Neck 0.022937 0.049614 0.008061 0.007865 0.010988 0.041380 0.001446 0.006474 0.019111 0.048522 0.005722 0.007317
Bud Site 0.003828 0.016979 0.007908 0.030926 0.001409 0.010440 0.002410 0.039921 0.003053 0.016112 0.005964 0.034472
Cell Periphery 0.000144 0.000141 0.000213 0.000188 0.000155 0.000133 0.000193 0.000311 0.000147 0.000140 0.000206 0.000236
Cytoplasm 0.536685* 0.347678* 0.211599* 0.492137* 0.488685* 0.244717* 0.382794* 0.505608* 0.521312* 0.334027* 0.272125* 0.497447*
Cytoplasmic Foci 0.150504 0.288484* 0.016545 0.020496 0.228088* 0.335657* 0.031446 0.030011 0.175350 0.294738* 0.021813 0.024247
Eisosomes 0.000153 0.000177 0.000070 0.000048 0.000166 0.000211 0.000097 0.000055 0.000157 0.000181 0.000079 0.000051
Endoplasmic Reticulum 0.000702 0.000695 0.005166 0.001493 0.000455 0.000584 0.001956 0.001183 0.000623 0.000680 0.004031 0.001371
Endosome 0.007483 0.036110 0.003685 0.005659 0.008271 0.047621 0.004167 0.008463 0.007735 0.037636 0.003855 0.006764
Golgi 0.000997 0.009187 0.000138 0.002882 0.001284 0.008573 0.000088 0.005361 0.001089 0.009106 0.000120 0.003859
Lipid Particles 0.006080 0.009292 0.003215 0.001248 0.008650 0.014464 0.002657 0.001298 0.006903 0.009978 0.003018 0.001268
Mitochondria 0.002921 0.020860 0.000648 0.004372 0.002855 0.010686 0.000275 0.003965 0.002900 0.019511 0.000516 0.004211
Mitotic Spindle 0.000365 0.007323 0.001293 0.028760 0.000607 0.016401 0.002498 0.045737 0.000442 0.008526 0.001719 0.035452
None 0.004388 0.004371 0.005293 0.005150 0.003762 0.001138 0.011498 0.007600 0.004188 0.003942 0.007487 0.006116
Nuclear Periphery 0.000385 0.000462 0.002219 0.001840 0.000531 0.000551 0.001321 0.001020 0.000431 0.000474 0.001902 0.001517
Nuclear Periphery Foci 0.000193 0.000287 0.001357 0.001433 0.000240 0.000490 0.001288 0.000679 0.000208 0.000314 0.001333 0.001136
Nucleolus 0.001143 0.003163 0.001672 0.001251 0.001222 0.003147 0.001011 0.000585 0.001168 0.003161 0.001438 0.000988
Nucleus 0.206558* 0.096931 0.661070* 0.293742* 0.204347* 0.114708 0.424882* 0.212564* 0.205850* 0.099288 0.577566* 0.261741*
Peroxisomes 0.002773 0.025359 0.000467 0.001139 0.004027 0.050368 0.000304 0.001721 0.003174 0.028675 0.000409 0.001369
Vacuole 0.051074 0.079522 0.068691 0.095656 0.033628 0.095918 0.129343 0.120780 0.045487 0.081696 0.090134 0.105560
Vacuole Periphery 0.000629 0.002904 0.000360 0.002219 0.000579 0.002395 0.000188 0.005763 0.000613 0.002836 0.000299 0.003616

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.52 -10.13 -3.30 -1.57 -1.38 -2.64 -6.98 -4.40 -0.90 -2.89 -8.00 -11.54 -4.46 -1.74 -2.10
Bud Neck -7.74 7.89 11.22 13.68 0.90 -4.07 7.63 4.13 4.89 -5.03 -9.56 10.00 11.99 14.65 -0.41
Bud Site -9.04 -2.19 -7.19 -0.54 -4.49 -4.03 -1.53 -7.16 -3.23 -5.86 -10.19 -2.55 -10.01 -1.75 -7.06
Cell Periphery 0.25 -2.43 0.39 0.18 2.51 1.27 -0.65 -0.73 -1.12 -0.54 0.81 -2.53 -0.58 -0.90 1.62
Cytoplasm 19.65 34.80 17.65 -4.03 -18.38 13.19 10.15 6.15 -8.93 -5.60 21.63 31.96 17.85 -7.13 -17.30
Cytoplasmic Foci -18.01 37.10 39.75 39.73 -0.33 -5.32 31.92 33.44 17.55 0.20 -16.56 47.97 50.67 43.04 -0.29
Eisosomes -2.19 8.92 16.12 12.74 6.11 -1.41 7.14 13.80 4.93 4.85 -2.37 11.29 20.85 13.51 7.75
Endoplasmic Reticulum 0.02 -13.87 -13.92 -14.50 10.12 -0.75 -7.91 -8.88 -7.15 3.90 -0.64 -15.50 -16.14 -15.42 10.72
Endosome -12.61 5.58 7.23 14.70 2.41 -4.51 5.67 4.03 4.98 -0.41 -13.18 7.74 7.79 15.04 1.32
Golgi -8.90 10.56 -0.32 7.86 -2.24 -2.38 5.08 -1.07 2.03 -2.72 -8.97 10.34 -1.07 7.73 -3.51
Lipid Particles -3.67 4.54 11.38 10.35 5.43 -1.24 6.70 8.27 3.02 4.97 -3.12 7.81 13.90 9.89 6.71
Mitochondria -8.65 5.85 1.15 8.85 -2.57 -2.77 5.16 1.23 3.11 -2.88 -9.05 7.72 1.66 9.42 -3.67
Mitotic Spindle -4.63 -2.45 -8.32 -4.53 -6.97 -2.34 -1.56 -7.39 -1.57 -3.43 -5.10 -2.28 -11.11 -5.70 -7.03
None 0.03 -0.01 -0.11 -0.10 -0.08 3.77 -1.36 -1.68 -5.28 0.73 0.29 -1.20 -1.23 -1.06 0.59
Nuclear Periphery -1.59 -19.28 -11.30 -10.74 1.44 -0.26 -10.94 -12.07 -7.93 0.55 -1.03 -21.99 -14.89 -14.16 1.79
Nuclear Periphery Foci -3.17 -10.59 -4.92 -4.31 3.42 -1.53 -7.92 -8.77 -2.03 5.05 -3.28 -13.22 -6.72 -5.56 5.47
Nucleolus -3.37 -0.75 2.05 3.89 1.83 -1.62 1.86 3.09 2.20 1.19 -3.66 0.10 3.58 4.52 2.15
Nucleus 20.41 -34.48 -18.38 -31.81 21.92 6.64 -11.58 -6.27 -10.24 7.73 21.80 -32.36 -18.06 -33.12 21.31
Peroxisomes -13.48 8.97 7.82 14.95 -1.67 -5.04 10.23 7.91 5.42 -2.70 -13.48 13.17 10.80 14.92 -3.05
Vacuole -7.91 -8.49 -23.25 -16.24 -8.12 -6.27 -12.27 -24.56 -8.30 0.04 -10.95 -14.32 -33.12 -20.71 -5.74
Vacuole Periphery -9.88 4.82 -1.14 5.64 -2.24 -2.43 5.63 -2.10 -0.05 -2.58 -10.03 7.16 -2.38 3.09 -3.28
Standard name
Human Ortholog
Description Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
Localization
Cell Percentages cytoplasm (36%), vacuole (1%), mixed (40%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ald3

Ald3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ald3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available