Standard name
Human Ortholog
Description Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.95 0.98 0.97 0.88 – 0.65 0.64 0.62 0.61 0.6 0.99 0.99 0.99 0.97 1.0 0.95 0.94 0.9 0.9 0.93 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.19 0.05 0.23 – 0.28 0.36 0.34 0.43 0.42 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.16 0.17 0.16 0.1 0.09 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1
Bud 0 0 2 1 2 0 – 9 7 10 12 7 0 0 1 0 0 0 0 1 0 0 1 1
Bud Neck 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Site 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 2 0 1 – 9 6 10 13 9 0 1 1 1 0 0 1 0 1 0 0 0
Cytoplasm 198 154 212 350 330 511 – 472 344 356 323 269 227 340 378 30 23 21 191 142 208 103 204 251
Endoplasmic Reticulum 4 0 1 0 0 1 – 6 0 2 3 3 1 2 1 1 0 2 0 0 1 0 0 0
Endosome 0 0 0 0 1 0 – 0 0 1 0 0 0 0 1 0 0 0 2 2 1 0 1 1
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1
Mitochondria 3 5 21 68 16 137 – 200 196 196 228 186 1 1 0 0 0 1 3 1 8 1 8 7
Nucleus 0 1 2 0 2 3 – 23 14 18 28 19 1 0 0 1 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 – 12 17 13 3 7 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 1 0 – 1 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 0 1 0 0 4 14 – 116 93 91 53 39 0 1 0 1 0 1 2 3 1 1 1 4
Unique Cell Count 203 157 222 357 341 583 725 538 574 531 447 229 344 380 31 23 22 205 158 232 111 227 277
Labelled Cell Count 206 162 241 421 357 668 848 677 698 665 541 230 345 382 34 23 25 205 158 232 111 227 277


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.5 3.5 3.7 5.1 4.6 – 5.6 6.0 6.5 6.3 6.9 4.3 5.7 5.9 6.1 6.8 6.2 4.3 4.8 5.0
Std Deviation (1e-4) 0.6 1.4 0.8 1.4 1.6 1.6 – 2.0 3.1 3.6 3.6 4.3 0.9 1.4 1.9 1.3 2.3 1.7 0.8 1.3 1.2
Intensity Change (Log2) – – – 0.06 0.53 0.41 – 0.66 0.76 0.88 0.85 0.97 0.3 0.7 0.75 0.79 0.95 0.81 0.28 0.45 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 0.8 -3.3 – -8.8 -8.9 -9.4 -9.6 -9.6 2.4 2.5 3.4 0.3 1.0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -2.2 0 – 5.6 7.5 7.0 8.9 8.5 0 -5.5 -6.1 0 0 0
Nucleus 0 0 0 – 1.8 0 1.8 2.8 2.3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 6.4 6.6 6.3 4.9 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8309 1.1252 0.9246 0.4931 0.6912 0.7186 2.1142 2.6796 2.0798 3.5383 3.8372 1.9764 2.4905 3.0892 2.6368 2.8002 2.8675 2.6833
Actin 0.0402 0.0013 0.0177 0.0371 0.0086 0.0028 0.0198 0.0055 0.011 0.0346 0.0106 0.0142 0.0302 0.0004 0.0078 0.0003 0.0016 0.0115
Bud 0.0072 0.0044 0.0018 0.0007 0.0141 0.0013 0.0016 0.0074 0.0074 0.0016 0.0007 0.0043 0.0009 0.0002 0.0002 0.0002 0.001 0.0003
Bud Neck 0.0078 0.0005 0.0082 0.0012 0.0007 0.0076 0.0012 0.0004 0.0018 0.0064 0.0004 0.0018 0.0017 0.0001 0.001 0.0003 0.0003 0.0012
Bud Periphery 0.012 0.0014 0.0024 0.0007 0.0088 0.0009 0.002 0.0031 0.0068 0.0016 0.0006 0.0063 0.002 0.0002 0.0002 0.0001 0.0005 0.0003
Bud Site 0.0096 0.0176 0.0125 0.0023 0.0057 0.0002 0.0069 0.0158 0.0088 0.0073 0.0007 0.0009 0.0024 0.0024 0.0033 0.0002 0.0003 0.0003
Cell Periphery 0.0005 0.0003 0.0003 0.0001 0.0008 0 0.0005 0.0003 0.0006 0.0006 0.0001 0.0003 0.0005 0.0001 0.0001 0.0001 0 0.0001
Cytoplasm 0.3478 0.532 0.4768 0.447 0.3864 0.5306 0.5484 0.7198 0.6565 0.7152 0.6087 0.6959 0.5899 0.6842 0.5634 0.7591 0.6242 0.6783
Cytoplasmic Foci 0.0275 0.0094 0.0176 0.0433 0.0272 0.0186 0.0369 0.0065 0.0148 0.0118 0.0176 0.0142 0.0138 0.003 0.0076 0.0048 0.0251 0.0068
Eisosomes 0.0004 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0002 0 0 0.0002
Endoplasmic Reticulum 0.0084 0.0021 0.0033 0.0014 0.0014 0.0015 0.0079 0.002 0.0042 0.004 0.0091 0.0021 0.0053 0.0014 0.0022 0.002 0.0027 0.0015
Endosome 0.0321 0.003 0.0109 0.0087 0.0145 0.0057 0.0437 0.0014 0.0209 0.0219 0.0416 0.0142 0.007 0.001 0.007 0.0019 0.042 0.0093
Golgi 0.0086 0.0002 0.0018 0.0073 0.0032 0.0006 0.0128 0.0005 0.0142 0.0086 0.0077 0.0077 0.0059 0.0001 0.005 0.0002 0.0074 0.003
Lipid Particles 0.0099 0.0002 0.0016 0.0103 0.0226 0.001 0.0139 0.0001 0.0057 0.0032 0.0024 0.0042 0.0042 0.0001 0.0056 0.0001 0.0064 0.0063
Mitochondria 0.0054 0.0004 0.0009 0.0057 0.0038 0.0003 0.0099 0.0008 0.0111 0.0023 0.0396 0.0129 0.0178 0.0001 0.0168 0.0002 0.0047 0.0017
None 0.4361 0.4171 0.4261 0.3623 0.4467 0.4148 0.2274 0.2289 0.2139 0.1067 0.1746 0.203 0.2985 0.3038 0.3672 0.2262 0.2502 0.2693
Nuclear Periphery 0.016 0.001 0.0034 0.001 0.006 0.0047 0.0187 0.001 0.002 0.0079 0.004 0.004 0.0022 0.0003 0.0012 0.0005 0.0049 0.0027
Nucleolus 0.0019 0.0004 0.0005 0.0002 0.0011 0.0001 0.0014 0.0001 0.0003 0.0009 0.0001 0.0006 0.0001 0.0001 0.0004 0 0.0002 0.0001
Nucleus 0.0078 0.0039 0.0044 0.0061 0.0062 0.0039 0.0118 0.0034 0.0041 0.0093 0.002 0.005 0.0017 0.0013 0.0033 0.0026 0.0023 0.0033
Peroxisomes 0.0063 0.0003 0.0014 0.0581 0.025 0.0011 0.0185 0.0008 0.0057 0.0034 0.0293 0.0013 0.012 0 0.0038 0.0001 0.0088 0.0012
Punctate Nuclear 0.0093 0.003 0.0065 0.0048 0.0147 0.003 0.0048 0.0012 0.0016 0.0014 0.001 0.0041 0.0014 0.0007 0.0024 0.0006 0.0147 0.0013
Vacuole 0.0035 0.0013 0.0015 0.0012 0.0022 0.001 0.0093 0.0009 0.0072 0.046 0.0393 0.0021 0.0018 0.0004 0.0012 0.0007 0.0012 0.0007
Vacuole Periphery 0.0017 0.0001 0.0004 0.0002 0.0004 0.0002 0.0025 0.0001 0.0011 0.0053 0.0095 0.0008 0.0004 0 0.0002 0.0001 0.0014 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
20 1191 926 1298 1746 994 1827 1658 1766 2185 2753 2956

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2546.21 742.27 1116.12 949.70 681.25 748.34 840.25 962.54 702.37 745.03 933.04 956.90
Standard Deviation 1053.43 121.77 218.51 210.21 85.60 93.90 314.39 159.88 242.40 110.01 314.07 183.79
Intensity Change Log 2 — -1.778335 -1.189859 -1.422808 — 0.135510 0.302634 0.498662 — -1.114496 -0.722220 -0.755136

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001467 0.000320 0.000419 0.001082 0.000069 0.000249 0.000178 0.001352 0.000085 0.000288 0.000259 0.001233
Bud Neck 0.003010 0.029560 0.001964 0.005093 0.015901 0.036205 0.006159 0.009729 0.015755 0.032583 0.004748 0.007693
Bud Site 0.022455 0.012820 0.004030 0.025231 0.002755 0.011082 0.025273 0.036229 0.002978 0.012029 0.018127 0.031400
Cell Periphery 0.001409 0.000141 0.000132 0.000173 0.000133 0.000236 0.000140 0.000152 0.000147 0.000185 0.000137 0.000161
Cytoplasm 0.757300* 0.475605* 0.589366* 0.518523* 0.480247* 0.388847* 0.683188* 0.600177* 0.483384* 0.436137* 0.651630* 0.564322*
Cytoplasmic Foci 0.032394 0.232133* 0.010743 0.029832 0.241712* 0.305959* 0.022014 0.021907 0.239341* 0.265718* 0.018223 0.025387
Eisosomes 0.000088 0.000112 0.000024 0.000047 0.000217 0.000244 0.000029 0.000033 0.000216 0.000172 0.000028 0.000039
Endoplasmic Reticulum 0.000265 0.000616 0.002791 0.001639 0.000660 0.000452 0.000469 0.001040 0.000656 0.000541 0.001250 0.001303
Endosome 0.000512 0.024142 0.002273 0.011239 0.011593 0.029020 0.002616 0.008600 0.011468 0.026361 0.002501 0.009759
Golgi 0.003066 0.003726 0.000141 0.007673 0.001371 0.004424 0.000229 0.008798 0.001390 0.004044 0.000200 0.008304
Lipid Particles 0.001832 0.006735 0.000949 0.001631 0.011276 0.009428 0.000649 0.001082 0.011169 0.007960 0.000750 0.001323
Mitochondria 0.000206 0.008086 0.000287 0.008213 0.002721 0.011318 0.000857 0.004820 0.002693 0.009557 0.000665 0.006310
Mitotic Spindle 0.009213 0.004404 0.006709 0.053126 0.000081 0.001110 0.020162 0.039044 0.000184 0.002906 0.015637 0.045227
None 0.003008 0.003496 0.007822 0.003840 0.002415 0.002834 0.008178 0.007160 0.002422 0.003195 0.008059 0.005702
Nuclear Periphery 0.000275 0.000337 0.000881 0.001791 0.000534 0.000351 0.000551 0.000816 0.000531 0.000344 0.000662 0.001244
Nuclear Periphery Foci 0.002944 0.000369 0.002648 0.002547 0.000296 0.000267 0.000397 0.000788 0.000326 0.000323 0.001154 0.001561
Nucleolus 0.000086 0.001958 0.000244 0.000359 0.000874 0.002123 0.000463 0.000340 0.000866 0.002033 0.000389 0.000348
Nucleus 0.140013* 0.099914 0.301668* 0.119393 0.172881* 0.106583 0.146542* 0.131304* 0.172508* 0.102948 0.198720* 0.126074
Peroxisomes 0.000221 0.014592 0.000292 0.003702 0.005443 0.019054 0.000623 0.004140 0.005384 0.016622 0.000512 0.003948
Vacuole 0.019962 0.078769 0.066454 0.192135 0.048082 0.068322 0.080795 0.119159 0.047764 0.074016 0.075971 0.151203
Vacuole Periphery 0.000275 0.002163 0.000161 0.012732 0.000738 0.001893 0.000488 0.003332 0.000733 0.002040 0.000378 0.007460

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.86 2.32 2.27 -1.47 0.07 -4.91 -5.51 -3.57 -2.12 -2.73 -7.36 -7.55 -4.93 -2.49 -2.52
Bud Neck -5.14 1.88 1.41 10.43 -2.39 -6.64 8.51 9.23 10.68 0.81 -7.87 10.13 10.20 14.87 0.11
Bud Site 1.14 2.42 1.09 -0.12 -4.92 -5.50 -8.07 -6.95 -1.90 1.47 -8.66 -8.02 -8.71 -1.54 0.03
Cell Periphery 4.13 4.09 4.24 1.90 5.79 -3.10 0.98 2.85 4.02 1.42 -1.57 1.58 4.85 4.29 3.40
Cytoplasm 1.44 0.64 2.07 3.57 7.88 8.69 -14.79 -0.03 -8.33 13.91 5.40 -12.64 2.74 -3.04 16.67
Cytoplasmic Foci -11.32 2.59 2.22 33.79 -4.04 -7.36 43.94 45.39 41.01 4.09 -3.58 44.79 45.22 52.08 1.59
Eisosomes -1.65 0.77 0.39 11.82 -5.32 -1.34 17.66 17.05 12.32 -4.17 3.31 17.75 16.88 15.77 -6.86
Endoplasmic Reticulum -2.14 -7.12 -8.89 -7.30 4.39 1.49 -0.51 -5.38 -9.39 -8.86 0.84 -5.15 -6.10 -11.48 1.08
Endosome -13.17 -6.20 -5.39 10.90 -2.30 -7.42 10.48 8.71 11.86 -1.24 -8.98 10.40 8.67 16.18 -2.53
Golgi -1.26 1.58 0.02 1.78 -2.38 -4.52 7.21 -1.06 2.52 -2.91 -6.07 7.43 -1.28 3.03 -3.75
Lipid Particles 0.44 1.80 1.78 4.35 -0.49 1.46 13.28 12.80 8.10 -3.02 3.03 13.15 12.79 8.27 -3.40
Mitochondria -5.91 -0.85 -3.05 3.74 -2.97 -5.01 6.62 2.61 5.66 -1.89 -6.26 7.49 1.77 6.82 -3.37
Mitotic Spindle 0.90 0.54 -3.99 -7.75 -7.12 -2.76 -9.46 -8.88 -8.30 0.73 -4.12 -10.21 -12.61 -10.86 -3.59
None 0.26 -2.25 -0.30 -0.80 2.67 -0.37 -4.26 -4.88 -3.18 0.85 -0.90 -4.87 -4.01 -3.33 2.28
Nuclear Periphery 2.39 -3.28 -4.70 -8.39 -3.05 2.07 -6.60 -15.76 -14.57 -12.00 3.05 -8.85 -12.55 -15.44 -7.97
Nuclear Periphery Foci 3.46 1.41 2.60 -5.55 5.35 0.38 -2.93 -8.55 -11.60 -7.49 0.23 -9.84 -7.67 -9.09 1.50
Nucleolus -5.64 -4.55 -3.28 4.93 -1.25 -3.06 4.08 5.73 4.66 2.02 -4.30 4.87 5.51 6.81 1.12
Nucleus 2.39 -1.84 1.62 -6.92 20.04 10.70 0.16 -2.11 -12.27 -2.17 13.86 -8.38 1.45 -14.38 10.74
Peroxisomes -10.04 -0.77 -1.70 8.71 -1.53 -6.68 7.81 5.34 9.02 -1.19 -8.37 7.96 5.86 12.55 -1.85
Vacuole 0.35 -0.37 -6.43 -28.07 -22.44 -5.64 -17.26 -30.97 -25.87 -15.86 -9.27 -18.89 -44.09 -37.45 -26.50
Vacuole Periphery -5.29 0.39 -3.45 -1.25 -3.51 -4.63 3.87 -0.41 2.56 -1.57 -5.59 5.28 -2.76 0.13 -3.79
Standard name
Human Ortholog
Description Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
Localization
Cell Percentages cytoplasm (16%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pai3

Pai3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pai3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available