Standard name
Human Ortholog
Description Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.34 0.53 0.48 0.59 0.4 0.5 0.42 0.34 0.33 0.27 0.28 0.34 0.54 0.53 0.86 0.88 0.85 0.14 0.3 0.35 0.29 0.4 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.32 0.38 0.47 0.6 0.38 0.63 0.56 0.57 0.7 0.77 0.79 0.77 0.15 0.08 0.33 0.06 0 0 0.11 0.08 0.14 0 0.05 0.08
Nucleus 0.24 0.07 0 0.06 0 0.07 0.08 0.11 0.08 0.1 0.11 0.13 0.14 0.17 0.06 0 0 0 0.25 0.23 0.08 0.26 0.13 0.08
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.06 0.07 0.06
Vac/Vac Membrane 0.26 0.47 0.05 0 0.09 0.06 0 0 0 0 0 0.06 0.35 0.23 0.21 0.15 0.15 0.16 0.25 0.25 0.29 0.3 0.24 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 3 5
Bud 2 1 0 3 3 3 3 7 5 6 5 8 0 1 3 0 0 0 2 1 0 1 4 7
Bud Neck 0 0 0 0 1 3 4 2 2 0 1 3 0 0 0 1 0 0 0 1 0 0 5 5
Bud Site 0 0 0 1 1 1 7 10 2 4 7 15 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 37 29 42 75 133 87 191 124 70 93 50 87 22 97 124 145 131 149 30 32 35 29 86 107
Endoplasmic Reticulum 0 1 1 0 0 1 0 0 0 0 0 0 1 2 0 13 8 11 1 1 0 0 1 2
Endosome 7 2 0 0 7 1 3 1 0 0 0 0 5 12 3 3 1 5 13 0 4 0 3 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2
Mitochondria 47 33 37 93 86 137 216 168 143 213 144 239 10 15 78 10 5 4 24 8 13 2 10 23
Nucleus 36 6 1 10 9 15 30 32 17 28 21 39 9 30 14 2 5 1 57 24 7 25 28 23
Nuclear Periphery 0 1 0 1 0 0 0 0 0 1 0 4 0 0 0 0 0 0 3 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Peroxisomes 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 2 3 3 3 1 3 2 3 0 0 0 0 0 0 20 4 3 6 14 19
Vac/Vac Membrane 38 40 4 0 21 12 16 6 4 7 5 20 23 41 49 26 23 29 57 27 28 30 53 83
Unique Cell Count 148 86 79 155 225 216 383 294 204 278 183 309 65 180 233 169 149 176 228 107 101 101 217 307
Labelled Cell Count 168 114 87 184 264 263 475 354 244 356 235 418 70 198 271 201 173 200 228 107 101 101 217 307


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.9 3.2 3.2 3.6 3.2 3.2 3.2 2.7 2.8 2.9 2.8 4.5 4.3 3.7 5.0 5.2 5.8 4.1 4.2 4.6
Std Deviation (1e-4) 1.0 1.5 0.6 1.3 1.8 1.5 1.8 1.5 1.3 1.7 1.1 1.7 1.2 0.8 1.0 1.1 0.9 1.8 0.8 1.4 1.5
Intensity Change (Log2) – – – 0.01 0.16 -0.01 -0.0 -0.0 -0.23 -0.2 -0.17 -0.21 0.5 0.41 0.19 0.65 0.68 0.84 0.35 0.4 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.3 2.3 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0275 0.0002 0.0086 0.0223 0.083 0.0147 0.0338 0.0121 0.0219 0.0024 0.0003 0.0042 0.0001 0.0002 0.0002 0.0007 0.0001 0.0004
Bud 0.0002 0.0001 0.0003 0.0003 0.0008 0.0012 0.0037 0.0007 0.0009 0.0003 0.0001 0.0014 0 0.0002 0 0 0 0.0002
Bud Neck 0.002 0.0003 0.0006 0.0008 0.0035 0.0074 0.0008 0.0003 0.0005 0.0007 0.0001 0.0033 0.0002 0 0.0001 0.0001 0.0001 0.0009
Bud Periphery 0.0002 0.0001 0.0004 0.0005 0.0026 0.0012 0.0036 0.0006 0.0013 0.0003 0 0.0027 0 0.0002 0 0 0 0.0001
Bud Site 0.0019 0.0011 0.0022 0.0018 0.0055 0.0007 0.0084 0.0014 0.0068 0.0012 0.0001 0.0003 0.0005 0.0001 0.0002 0 0.0001 0.0001
Cell Periphery 0.0001 0.0002 0.0002 0.0003 0.0002 0.0001 0.0007 0.0001 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0 0
Cytoplasm 0.1064 0.2813 0.1669 0.1837 0.1614 0.2708 0.1907 0.3558 0.3259 0.2319 0.2295 0.4098 0.0278 0.0219 0.1545 0.0895 0.0504 0.2266
Cytoplasmic Foci 0.0274 0.0111 0.009 0.006 0.01 0.0101 0.0434 0.0114 0.0121 0.0179 0.0041 0.0106 0.0031 0.0063 0.0042 0.0015 0.004 0.0117
Eisosomes 0.0008 0.0001 0.0005 0.0007 0.0003 0.0002 0.0007 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0002 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0029 0.0081 0.0048 0.0064 0.0034 0.0036 0.0112 0.0048 0.0038 0.0019 0.0046 0.0034 0.0005 0.0007 0.0035 0.0021 0.0006 0.0028
Endosome 0.0073 0.0035 0.0044 0.0068 0.0084 0.0033 0.0159 0.0032 0.0065 0.0289 0.0026 0.0032 0.0004 0.0003 0.0021 0.0009 0.001 0.0034
Golgi 0.003 0.0002 0.0011 0.0032 0.0027 0.0006 0.0063 0.0002 0.0057 0.007 0.0001 0.0007 0 0.0001 0.0001 0.0001 0 0.0001
Lipid Particles 0.0216 0.0007 0.0048 0.0025 0.0044 0.0008 0.0094 0.0005 0.0022 0.005 0.0001 0.0018 0.0005 0.0005 0.0003 0.0002 0.0001 0.0003
Mitochondria 0.001 0.0004 0.0013 0.006 0.0111 0.0007 0.0577 0.0006 0.0153 0.0103 0.0005 0.0081 0.0004 0.0003 0.0005 0.001 0.0006 0.0005
None 0.6451 0.5695 0.6106 0.6209 0.4866 0.4914 0.427 0.4795 0.4053 0.4855 0.6353 0.3422 0.9027 0.9646 0.7549 0.8679 0.6976 0.6328
Nuclear Periphery 0.0046 0.0201 0.011 0.007 0.0323 0.0053 0.0275 0.0058 0.0086 0.0034 0.0084 0.0118 0.0016 0.0007 0.0054 0.0062 0.0011 0.0046
Nucleolus 0.002 0.0017 0.0034 0.0028 0.0044 0.0018 0.0038 0.0008 0.0014 0.0009 0.0003 0.0012 0.008 0.0003 0.0021 0.0005 0.0014 0.001
Nucleus 0.114 0.0741 0.147 0.1113 0.1244 0.1663 0.0911 0.1041 0.1432 0.1779 0.1101 0.1757 0.0329 0.0006 0.0612 0.0253 0.2405 0.0847
Peroxisomes 0.0148 0.0002 0.0058 0.0007 0.0293 0.0005 0.0139 0.0007 0.0038 0.0064 0.0001 0.0011 0.0001 0.0003 0.0001 0.0001 0.0001 0.0002
Punctate Nuclear 0.0157 0.0235 0.0149 0.0133 0.0209 0.0172 0.0322 0.0155 0.0253 0.0102 0.0023 0.0163 0.02 0.0021 0.0088 0.0029 0.0014 0.0283
Vacuole 0.0011 0.0031 0.002 0.0022 0.0026 0.0016 0.0112 0.0018 0.0086 0.0052 0.001 0.0015 0.0007 0.0003 0.0013 0.0004 0.0005 0.0013
Vacuole Periphery 0.0002 0.0003 0.0004 0.0006 0.0024 0.0002 0.0068 0.0002 0.0007 0.0026 0.0003 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1169 7.6842 12.4408 10.765 9.6252 6.2481 10.5948 11.8385 11.9859 14.4302
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
814 779 911 1033 956 1465 607 1907 1770 2244 1518 2940

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 543.80 746.31 909.37 779.65 606.69 718.09 840.69 767.52 577.77 727.89 881.91 771.78
Standard Deviation 67.82 94.59 126.63 100.27 88.63 105.10 91.47 109.83 85.68 102.46 118.75 106.73
Intensity Change Log 2 — 0.456699 0.741791 0.519750 — 0.243205 0.470614 0.339245 — 0.348061 0.605156 0.427383

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000040 0.000379 0.000625 0.001497 0.000051 0.001632 0.000394 0.000583 0.000046 0.001197 0.000532 0.000904
Bud Neck 0.005274 0.010218 0.006346 0.009952 0.004488 0.027187 0.004889 0.017031 0.004850 0.021296 0.005763 0.014543
Bud Site 0.001148 0.004429 0.010714 0.033530 0.001956 0.014186 0.002844 0.017969 0.001585 0.010799 0.007567 0.023437
Cell Periphery 0.000177 0.000145 0.000150 0.000231 0.000135 0.000130 0.000081 0.000168 0.000154 0.000135 0.000123 0.000190
Cytoplasm 0.331574* 0.261864* 0.070527 0.258940* 0.312889* 0.322924* 0.043024 0.219760* 0.321482* 0.301727* 0.059529 0.233527*
Cytoplasmic Foci 0.065356 0.108786 0.003059 0.010314 0.093611 0.100853 0.000854 0.003496 0.080617 0.103607 0.002177 0.005892
Eisosomes 0.000078 0.000084 0.000040 0.000047 0.000070 0.000069 0.000029 0.000026 0.000074 0.000074 0.000036 0.000034
Endoplasmic Reticulum 0.001006 0.002169 0.006145 0.004469 0.000786 0.002161 0.005144 0.003745 0.000887 0.002164 0.005745 0.003999
Endosome 0.001762 0.008273 0.001084 0.005946 0.002484 0.009728 0.000570 0.001587 0.002152 0.009223 0.000878 0.003119
Golgi 0.000178 0.003612 0.000080 0.007932 0.000275 0.002862 0.000050 0.000358 0.000230 0.003122 0.000068 0.003019
Lipid Particles 0.005534 0.008349 0.001115 0.001410 0.007368 0.008315 0.000587 0.001039 0.006525 0.008327 0.000903 0.001169
Mitochondria 0.001806 0.011781 0.000893 0.003331 0.003310 0.014751 0.001664 0.002984 0.002618 0.013720 0.001201 0.003106
Mitotic Spindle 0.000066 0.015239 0.008185 0.029553 0.000422 0.020105 0.007641 0.021929 0.000258 0.018416 0.007967 0.024608
None 0.018934 0.007047 0.005258 0.008345 0.016590 0.004070 0.005645 0.007331 0.017668 0.005104 0.005413 0.007687
Nuclear Periphery 0.001219 0.002909 0.001230 0.002348 0.001217 0.002349 0.001435 0.003265 0.001218 0.002543 0.001312 0.002943
Nuclear Periphery Foci 0.000401 0.001011 0.000586 0.002629 0.000494 0.001145 0.000460 0.000571 0.000451 0.001099 0.000535 0.001294
Nucleolus 0.003756 0.004140 0.001222 0.000986 0.003345 0.004060 0.001230 0.000988 0.003534 0.004088 0.001225 0.000988
Nucleus 0.548622* 0.508216* 0.866316* 0.568197* 0.538313* 0.392334* 0.912747* 0.666770* 0.543054* 0.432562* 0.884882* 0.632135*
Peroxisomes 0.001312 0.012500 0.000318 0.001066 0.002375 0.009861 0.000345 0.000217 0.001886 0.010777 0.000329 0.000515
Vacuole 0.010718 0.027508 0.015920 0.048246 0.009481 0.058866 0.010221 0.026861 0.010050 0.047980 0.013641 0.034374
Vacuole Periphery 0.001039 0.001340 0.000188 0.001031 0.000339 0.002411 0.000149 0.003322 0.000661 0.002039 0.000173 0.002517

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.61 -15.24 -3.89 -2.45 -0.35 -2.67 -10.58 -5.82 1.78 0.51 -3.01 -18.37 -6.36 1.14 0.77
Bud Neck -3.20 -1.46 -3.44 0.16 -2.25 -9.25 -1.58 -7.29 4.33 -6.42 -9.73 -2.23 -8.04 4.16 -6.51
Bud Site -5.14 -3.69 -5.96 -4.61 -2.43 -6.11 -1.04 -5.76 0.87 -5.54 -7.20 -3.63 -8.51 -1.75 -3.92
Cell Periphery 1.59 2.13 0.28 -1.26 -1.59 0.53 3.02 0.59 0.15 -2.20 1.81 2.83 1.03 -0.67 -1.52
Cytoplasm 5.61 26.42 11.51 5.91 -16.81 -0.95 30.73 14.59 18.13 -22.64 2.44 39.66 18.90 18.12 -26.70
Cytoplasmic Foci -6.17 16.05 14.55 17.87 -6.19 -0.95 20.59 20.06 25.91 -6.61 -4.91 25.85 24.85 31.38 -6.92
Eisosomes -1.03 4.87 1.51 2.58 -4.62 0.30 6.38 10.08 9.68 2.43 -0.22 7.56 8.28 8.55 0.11
Endoplasmic Reticulum -3.54 -14.54 -13.80 -13.40 2.53 -4.15 -13.85 -18.22 -7.24 5.48 -4.95 -20.74 -20.58 -12.75 7.03
Endosome -6.08 1.28 -3.12 4.13 -4.81 -7.86 5.27 3.71 9.65 -2.41 -10.00 4.23 0.22 10.28 -4.66
Golgi -3.54 2.05 -1.94 0.46 -2.00 -3.86 6.18 1.50 3.99 -2.65 -5.23 5.75 -1.83 2.77 -2.15
Lipid Particles -3.05 6.80 5.88 9.79 -3.78 -0.76 10.16 9.61 9.00 -1.09 -2.33 11.98 11.27 12.23 -1.79
Mitochondria -4.55 2.40 -0.74 4.35 -2.81 -6.37 1.92 0.92 7.82 -2.17 -8.16 2.68 0.47 8.88 -4.35
Mitotic Spindle -4.64 -3.74 -7.42 -2.09 -3.88 -7.31 -2.54 -8.31 0.24 -3.75 -8.68 -4.48 -11.15 -1.02 -5.16
None 5.21 6.97 5.67 0.48 -2.65 5.82 5.39 4.68 -3.73 -2.16 8.14 8.70 7.30 -2.20 -3.71
Nuclear Periphery -2.21 -3.72 -13.09 -2.41 -11.96 -3.80 -6.25 -10.69 -5.19 -6.13 -4.03 -7.14 -15.34 -5.33 -11.51
Nuclear Periphery Foci -3.88 -2.51 -3.65 -3.04 -3.11 -2.70 -1.68 -1.31 2.13 1.02 -3.85 -3.00 -3.84 -1.89 -2.51
Nucleolus -0.57 5.24 5.28 7.48 0.22 -1.45 5.14 6.51 5.68 0.37 -1.33 7.50 8.21 8.07 0.73
Nucleus 2.46 -25.29 -3.45 -5.93 22.68 11.92 -30.23 -14.17 -30.73 20.43 11.45 -38.77 -13.66 -27.43 28.83
Peroxisomes -6.45 6.73 3.29 6.88 -2.24 -7.38 6.46 6.87 10.30 0.62 -10.01 8.61 8.03 12.13 -1.15
Vacuole -4.98 -4.23 -16.03 -13.06 -13.64 -15.99 -5.13 -14.87 3.49 -5.50 -15.75 -7.10 -20.80 -5.24 -13.43
Vacuole Periphery -0.64 2.01 1.25 3.25 -1.94 -2.92 4.36 -2.11 0.92 -2.48 -2.72 2.54 -1.22 1.42 -2.69
Standard name
Human Ortholog
Description Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (41%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ecm5

Ecm5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ecm5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available