Standard name
Human Ortholog
Description Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.11 0.08 0.15 0.39 0.52 0.71 0.62 0.71 0.72 0.78 0.27 0.29 0.6 0.23 0.17 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0.11 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0.05 0.05 0 0.05 0 0 0 0 0 0.07
Endosome 0.24 0.54 0.68 0.32 0.07 0.05 0 0 0 0 0 0.5 0.55 0.24 0.44 0.4 0.48 0.78 0.44 0.62 0.51 0.5 0.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Mitochondria 0 0.25 0.12 0.19 0.25 0.27 0.23 0.31 0.29 0.33 0.3 0 0 0 0 0 0 0.05 0.08 0.07 0.1 0.14 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.06 0.15 0.05 0 0 0 0 0 0 0 0.11 0.16 0.14 0 0.06 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.41 0.25 0.27 0.59 0.47 0.36 0.24 0.3 0.24 0.17 0.15 0.2 0.14 0 0.57 0.52 0.52 0.17 0.36 0.16 0.25 0.19 0.25
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 0 0 1 0 0 0 0 0 0 2 3 1 0 2 1 0 1 3 1 2 3
Bud 0 6 6 1 3 2 1 6 5 8 11 1 1 0 0 0 0 0 1 1 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 69 29 20 42 110 260 225 242 362 357 448 35 46 107 23 16 13 0 4 5 0 5 4
Endoplasmic Reticulum 20 8 1 5 6 2 0 0 0 14 5 16 10 9 5 2 5 0 7 3 0 5 11
Endosome 44 147 166 88 20 24 6 12 1 3 1 64 88 43 44 38 47 3 98 199 63 91 60
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 0 7 18 4 4 4
Mitochondria 3 69 29 53 72 136 72 123 146 165 170 0 1 0 4 4 2 0 18 24 12 25 14
Nucleus 0 0 1 9 4 6 5 4 2 0 2 1 0 1 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 1 3 3 0 1 1 0 2 2 2 0 0 0 0 0 1 0 0 1 0 1 0
Nucleolus 0 6 8 7 3 0 0 0 3 0 1 0 0 0 3 3 2 0 0 0 0 0 0
Peroxisomes 0 2 2 0 0 0 0 0 1 0 0 2 3 0 0 0 0 0 0 2 2 3 4
SpindlePole 2 17 36 13 2 4 1 3 2 0 1 14 26 25 4 6 5 0 0 4 3 3 4
Vac/Vac Membrane 77 69 66 162 133 181 76 118 122 84 87 26 22 4 56 49 51 0 79 52 31 34 38
Unique Cell Count 186 274 244 273 284 500 318 393 510 497 576 128 159 177 99 94 98 4 223 321 124 184 153
Labelled Cell Count 216 358 338 383 354 616 388 508 646 634 728 161 200 192 140 122 129 4 223 321 124 184 153


Endosome, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 4.8 4.9 5.2 4.6 4.0 4.0 3.8 3.7 3.7 3.7 7.0 6.3 5.9 8.3 8.0 9.0 7.6 7.7 8.1
Std Deviation (1e-4) 1.0 0.8 1.0 1.3 1.1 1.3 1.2 1.0 0.9 0.9 0.9 1.2 1.1 1.3 1.6 1.6 1.6 1.1 1.5 1.7
Intensity Change (Log2) 0.02 0.09 -0.06 -0.28 -0.27 -0.34 -0.38 -0.39 -0.38 0.52 0.38 0.29 0.78 0.73 0.89 0.65 0.66 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 1.7 7.7 11.4 14.7 13.2 16.1 16.3 18.5 4.3 4.9 11.3 3.1 1.6 0.7
Endoplasmic Reticulum 0 -0.8 0 0 0 0 0 0 0 3.8 1.7 1.2 0 0 0
Endosome 3.3 -5.1 0 0 0 0 0 0 0 -0.7 0.3 -6.2 -1.6 -2.2 -1.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.9 -1.6 0 0 0 0 0 0 0 -6.2 -6.7 -7.3 -4.5 -4.4 -5.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.8 0.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.2 -0.7 0 0 0 0 0 0 0 1.7 3.4 2.8 -0.8 0.1 -0.4
Vacuole 0.5 8.1 5.3 3.1 -0.4 1.4 -0.4 -2.8 -3.5 -1.1 -2.8 -6.5 5.7 4.8 4.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2183 3.5901 3.151 2.629 3.2683 3.1158 4.1343 5.4876 5.028 4.6511 4.6086 5.1894 1.9115 3.4634 2.6488 2.7748 2.5479 2.9484
Actin 0.0029 0.0001 0.0056 0.0352 0.0055 0.0002 0.0197 0.0001 0.002 0.0001 0.0096 0.0042 0.0008 0.0001 0.0001 0.0001 0 0.0001
Bud 0.0026 0.0001 0.0001 0 0.0001 0.0006 0.0002 0 0.0006 0 0.0002 0.0001 0.0001 0 0 0.0001 0 0
Bud Neck 0.0004 0.0004 0.0009 0.0003 0.0003 0.0009 0.0005 0.003 0.0006 0.0002 0.0003 0.001 0.0002 0.0003 0.0002 0.0001 0.0002 0.0003
Bud Periphery 0.0008 0.0002 0.0002 0 0.0002 0.0004 0.0003 0.0001 0.001 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Site 0.0062 0.0005 0.0007 0.0003 0.0003 0.0003 0.0009 0.0003 0.0045 0.0001 0.0004 0.0003 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0005 0.0002 0.0003 0 0.0001 0.0002 0.0007 0.0002 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0058 0.0039 0.0052 0.0012 0.0028 0.0075 0.0069 0.0014 0.0027 0.0193 0.0014 0.0058 0.0028 0.0033 0.003 0.001 0.0016 0.0012
Cytoplasmic Foci 0.0974 0.1374 0.1154 0.1645 0.0869 0.0843 0.098 0.1257 0.1369 0.1356 0.099 0.0688 0.0858 0.0995 0.1023 0.1025 0.0775 0.073
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0002 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0019 0.0018 0.002 0.0004 0.0042 0.0028 0.0068 0.0008 0.0028 0.0086 0.0005 0.0028 0.002 0.002 0.0016 0.0003 0.0013 0.0014
Endosome 0.4134 0.4104 0.4348 0.6085 0.5898 0.4459 0.3604 0.3331 0.3845 0.4728 0.4773 0.3206 0.5249 0.4276 0.5313 0.5484 0.6383 0.4327
Golgi 0.0654 0.0768 0.0815 0.0602 0.0698 0.0715 0.0612 0.0507 0.0809 0.0842 0.1234 0.0648 0.0574 0.0754 0.0874 0.0867 0.1016 0.0799
Lipid Particles 0.3121 0.2848 0.2626 0.0902 0.1528 0.2858 0.3581 0.425 0.2827 0.1929 0.184 0.4455 0.2483 0.3127 0.2023 0.2169 0.1096 0.3441
Mitochondria 0.0094 0.003 0.0211 0.001 0.0125 0.0048 0.0161 0.0018 0.0227 0.0043 0.0093 0.0122 0.0089 0.0145 0.0101 0.0039 0.0105 0.0051
None 0.007 0.0004 0.0044 0.0001 0.0028 0.0008 0.0072 0.0002 0.0086 0.0067 0.0001 0.0009 0.0045 0.0003 0.0065 0.0001 0.0001 0.0002
Nuclear Periphery 0.0148 0.0111 0.0032 0.0005 0.0142 0.0172 0.0072 0.0107 0.0077 0.0046 0.0005 0.0104 0.0135 0.0074 0.003 0.0004 0.0018 0.007
Nucleolus 0.0015 0.0072 0.0012 0.0001 0.0004 0.0017 0.0013 0.0021 0.0011 0.0008 0.0001 0.0027 0.0012 0.0029 0.0004 0.0001 0.0001 0.0008
Nucleus 0.0026 0.0017 0.0027 0.0001 0.001 0.0025 0.0009 0.0014 0.001 0.0005 0.0001 0.0015 0.001 0.0007 0.0004 0.0001 0.0002 0.0003
Peroxisomes 0.0225 0.017 0.0249 0.0255 0.0202 0.0141 0.0254 0.0151 0.033 0.0255 0.0377 0.0259 0.0102 0.0125 0.0145 0.023 0.0056 0.0091
Punctate Nuclear 0.0037 0.0056 0.0037 0.0005 0.0055 0.0017 0.0029 0.0026 0.0021 0.0008 0.0004 0.0017 0.003 0.002 0.0008 0.0005 0.0003 0.0009
Vacuole 0.0214 0.0284 0.0206 0.0082 0.022 0.0392 0.0165 0.019 0.0174 0.0275 0.0381 0.0199 0.0234 0.0215 0.0236 0.0095 0.0298 0.0262
Vacuole Periphery 0.0078 0.0088 0.0089 0.0032 0.0085 0.0175 0.0085 0.0066 0.0069 0.0152 0.0174 0.0103 0.0113 0.0169 0.0122 0.0061 0.0214 0.0175

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 65.2351 92.1951 89.019 82.4977 74.9138 78.2387 109.5035 122.4211 88.633 88.7204
Translational Efficiency 2.7738 2.5383 2.5355 1.8288 2.6398 2.3048 2.1897 2.5194 2.3983 2.0601

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1857 2000 97 1093 1950 849 1391 1668 3807 2849 1488 2761

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 764.78 846.20 978.05 1018.95 772.98 865.42 1041.51 1071.86 768.98 851.93 1037.37 1050.91
Standard Deviation 92.56 123.50 121.25 138.46 120.59 117.85 138.15 144.07 107.91 122.16 138.01 144.22
Intensity Change Log 2 0.145954 0.354863 0.413967 0.162969 0.430174 0.471613 0.154532 0.393211 0.443232

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000388 0.000499 0.003084 0.017104 0.000250 0.000473 0.010609 0.026228 0.000317 0.000491 0.010118 0.022616
Bud Neck 0.004773 0.001551 0.008775 0.003054 0.004796 0.001688 0.000670 0.001401 0.004785 0.001592 0.001199 0.002055
Bud Site 0.001845 0.002175 0.015592 0.015594 0.002081 0.002788 0.005882 0.009632 0.001966 0.002358 0.006515 0.011993
Cell Periphery 0.000130 0.000037 0.000146 0.000084 0.000089 0.000135 0.000075 0.000074 0.000109 0.000066 0.000080 0.000078
Cytoplasm 0.021554 0.010106 0.013185 0.009840 0.017861 0.005819 0.002222 0.006268 0.019662 0.008829 0.002936 0.007682
Cytoplasmic Foci 0.188465* 0.242847* 0.178342 0.246982* 0.186701* 0.137790 0.231613* 0.251521* 0.187561* 0.211540* 0.228140* 0.249724*
Eisosomes 0.000169 0.000038 0.000084 0.000064 0.000106 0.000050 0.000060 0.000087 0.000137 0.000041 0.000062 0.000078
Endoplasmic Reticulum 0.007693 0.002475 0.007154 0.002302 0.006288 0.002588 0.003583 0.001402 0.006973 0.002509 0.003816 0.001758
Endosome 0.408131* 0.403837* 0.378462* 0.358609* 0.455938* 0.535246* 0.409714* 0.388409* 0.432619* 0.442997* 0.407677* 0.376612*
Golgi 0.093175 0.155697* 0.024625 0.091219 0.085589 0.093544 0.222885* 0.159909* 0.089289 0.137175* 0.209961* 0.132716*
Lipid Particles 0.122562* 0.041745 0.044837 0.037057 0.091057 0.045954 0.031720 0.034688 0.106424* 0.042999 0.032575 0.035626
Mitochondria 0.018067 0.014007 0.000930 0.002723 0.011972 0.013627 0.001955 0.004458 0.014945 0.013894 0.001889 0.003771
Mitotic Spindle 0.001300 0.010977 0.053709 0.077917 0.002258 0.005945 0.013661 0.032912 0.001791 0.009477 0.016271 0.050728
None 0.000130 0.000265 0.000054 0.000073 0.000517 0.000219 0.000011 0.000119 0.000328 0.000251 0.000014 0.000101
Nuclear Periphery 0.000897 0.000835 0.001753 0.000781 0.000732 0.000531 0.000381 0.000483 0.000813 0.000745 0.000471 0.000601
Nuclear Periphery Foci 0.001887 0.003652 0.040964 0.014341 0.003201 0.005807 0.007477 0.006419 0.002560 0.004294 0.009660 0.009555
Nucleolus 0.000559 0.000713 0.003610 0.000128 0.001616 0.001152 0.000027 0.000343 0.001100 0.000844 0.000260 0.000258
Nucleus 0.002704 0.001047 0.006769 0.000433 0.002937 0.000807 0.000281 0.000270 0.002823 0.000975 0.000704 0.000334
Peroxisomes 0.008690 0.003596 0.030135 0.022420 0.006608 0.005199 0.005543 0.021984 0.007624 0.004074 0.007146 0.022157
Vacuole 0.100100 0.080352 0.179192 0.093838 0.102402 0.109502 0.044584 0.046742 0.101279 0.089038 0.053358 0.065386
Vacuole Periphery 0.016783 0.023551 0.008600 0.005435 0.017000 0.031138 0.007048 0.006652 0.016894 0.025812 0.007149 0.006170

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.48 -2.65 -6.81 -6.73 -5.25 -2.24 -6.79 -9.63 -9.54 -4.98 -1.04 -6.86 -11.73 -11.59 -5.15
Bud Neck 5.99 -2.38 2.25 -4.05 3.12 5.39 7.81 6.08 0.65 -4.51 8.09 8.97 6.34 -2.57 -4.02
Bud Site -1.00 -5.44 -8.14 -7.92 -0.25 -1.52 -4.35 -7.86 -6.83 -3.09 -1.44 -5.55 -11.30 -10.73 -4.88
Cell Periphery 4.98 -0.59 1.93 -4.56 2.06 -1.76 1.20 1.32 2.41 0.14 3.34 2.48 2.50 -1.55 0.02
Cytoplasm 6.48 1.92 4.12 -1.05 0.76 7.22 10.76 6.56 -1.04 -4.90 8.59 15.30 7.98 -0.20 -6.27
Cytoplasmic Foci -10.54 0.96 -7.57 0.56 -4.16 8.29 -7.51 -10.72 -17.17 -2.88 -5.88 -7.82 -13.19 -7.27 -3.23
Eisosomes 11.44 4.68 8.14 -4.19 1.71 5.72 4.79 1.95 -4.95 -3.72 13.00 9.00 7.24 -7.92 -2.66
Endoplasmic Reticulum 6.79 -0.19 6.73 0.15 3.02 4.97 3.20 6.65 3.08 4.28 8.16 4.62 9.71 2.16 4.33
Endosome 0.61 1.90 6.55 6.40 0.53 -7.33 5.40 8.35 13.78 2.91 -1.65 3.61 9.97 11.12 4.94
Golgi -12.09 13.33 1.31 12.33 -11.16 -1.46 -22.82 -13.84 -10.07 9.67 -12.35 -21.85 -10.62 1.73 13.56
Lipid Particles 16.79 8.82 17.22 1.36 0.97 8.20 13.48 12.59 2.33 -0.91 18.30 20.68 21.12 2.71 -1.07
Mitochondria 2.45 13.18 10.77 8.91 -2.28 -0.78 11.17 6.87 4.30 -2.57 0.88 15.09 12.27 9.15 -2.49
Mitotic Spindle -7.84 -4.14 -13.61 -11.72 -2.03 -2.16 -5.69 -11.21 -8.75 -6.02 -7.53 -7.17 -17.46 -14.14 -10.36
None -1.01 1.43 -0.48 0.59 -1.18 1.86 4.02 2.74 0.63 -1.20 0.80 4.65 2.20 0.96 -1.99
Nuclear Periphery 0.27 -2.42 -0.28 -0.51 2.30 1.48 2.69 1.62 0.25 -1.18 0.46 3.29 1.27 0.61 -2.15
Nuclear Periphery Foci -3.50 -4.45 -7.01 -5.98 2.93 -1.82 -3.45 -2.68 -0.56 0.58 -2.93 -5.76 -7.04 -5.17 -0.12
Nucleolus -0.79 -1.92 2.35 2.16 2.08 0.81 5.61 3.58 1.17 -1.53 1.03 3.96 3.89 1.97 -0.01
Nucleus 3.71 -1.62 2.72 0.02 1.91 3.95 5.12 5.50 1.39 -0.10 5.23 3.34 6.16 1.27 0.88
Peroxisomes 6.98 -3.81 -5.00 -7.05 1.23 1.68 1.26 -7.93 -8.53 -8.29 6.73 0.65 -9.12 -11.55 -8.77
Vacuole 3.87 -4.17 -1.08 -4.02 3.75 -1.02 12.30 10.62 8.50 -1.57 2.95 11.56 6.90 3.83 -4.51
Vacuole Periphery -4.62 4.06 11.09 13.98 1.41 -6.10 11.33 9.89 10.51 0.51 -7.45 13.28 14.22 16.82 1.51
Standard name
Human Ortholog
Description Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sso2

Sso2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sso2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available