Standard name
Human Ortholog
Description Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.97 0.98 0.93 0.88 0.85 – 0.77 0.81 0.74 0.74 0.98 0.98 0.99 0.95 0.95 0.96 0.95 0.96 0.93 0.88 0.89 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.09 – 0.26 0.23 0.33 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0 0.12 – 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 3 3
Bud 0 0 0 0 0 3 2 – 2 1 4 5 1 0 0 0 0 0 0 0 1 0 5 5
Bud Neck 0 0 1 0 0 1 1 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 6 2 5 0 12 – 5 8 3 4 1 4 15 3 4 12 0 0 0 0 2 1
Cytoplasm 260 144 227 164 214 146 307 – 253 240 168 197 254 320 356 209 134 124 256 145 220 175 283 266
Endoplasmic Reticulum 0 3 1 0 0 2 2 – 1 1 1 0 0 3 0 16 5 2 0 1 2 1 3 3
Endosome 0 0 0 0 2 0 3 – 0 3 0 1 1 1 0 0 0 1 2 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 1 2 3
Mitochondria 5 0 0 1 2 25 31 – 87 68 76 92 6 1 1 3 2 0 3 0 3 3 3 0
Nucleus 2 0 1 2 0 5 3 – 5 6 3 6 1 0 1 6 3 4 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 1 1 0 4 0 0 0 1 0 0 0 0 0 1 0 0
Nucleolus 0 0 1 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 2 – 2 2 0 0 0 0 0 1 0 0 0 0 0 2 2 1
Vac/Vac Membrane 2 0 5 3 17 2 45 – 19 18 6 10 0 2 6 8 1 1 0 0 0 4 4 4
Unique Cell Count 266 146 234 167 231 166 363 329 297 227 265 259 327 360 221 141 129 270 152 237 200 319 297
Labelled Cell Count 272 148 242 172 242 184 409 375 349 262 319 265 331 379 247 150 144 270 152 237 200 319 297


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.5 6.5 5.9 6.7 4.6 5.9 – 4.8 5.3 4.9 4.6 6.5 7.6 7.8 7.9 8.3 8.8 5.7 6.4 7.1
Std Deviation (1e-4) 0.9 0.7 1.7 1.5 1.5 1.1 1.5 – 1.3 1.8 1.3 1.0 1.6 1.4 1.6 1.7 1.7 2.0 1.7 1.9 2.2
Intensity Change (Log2) – – – -0.14 0.05 -0.51 -0.14 – -0.42 -0.3 -0.41 -0.48 0.01 0.22 0.27 0.29 0.36 0.44 -0.17 -0.01 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.3 0 0.5 – 0 0.1 0 0 0 0 1.0 0 0 2.8
Cytoplasm 0.8 -2.1 -3.6 -4.8 – -6.6 -5.7 -7.0 -7.1 0.8 0.6 1.7 -1.3 -1.0 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.8 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.6 – 0 7.8 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 4.4 – 2.1 2.2 0.4 1.1 0 0 0 0.9 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8416 0.5094 0.5072 0.1913 0.0457 0.3638 0.8022 1.854 1.8728 1.8788 1.9032 1.6958 3.7625 3.9369 3.5504 3.4704 5.0054 3.4189
Actin 0.0597 0.0004 0.0233 0.0002 0.0019 0.0007 0.0004 0.0004 0.0103 0.01 0.0085 0.0004 0.0587 0.0005 0.0006 0.0006 0.0004 0.0007
Bud 0.0007 0.0005 0.0013 0.0001 0.0001 0 0.0001 0.0001 0.0003 0.0103 0.0036 0.0007 0.0026 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0035 0.0003 0.0006 0.0001 0.0003 0.0009 0.0001 0.0001 0.0005 0.0006 0.0297 0.0006 0.016 0.0002 0.0002 0.0001 0.0945 0.0009
Bud Periphery 0.0009 0.0002 0.0072 0.0001 0.0001 0.0001 0.0002 0 0.0004 0.0107 0.0058 0.0002 0.0119 0.0001 0.0001 0 0.0003 0.0001
Bud Site 0.0032 0.0108 0.0034 0.0001 0.0001 0 0.0001 0.0005 0.0019 0.0033 0.0167 0.0001 0.0024 0.0006 0.0004 0.0001 0.0002 0.0001
Cell Periphery 0.0005 0.0003 0.0002 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0004 0.0001 0.0005 0.0005 0.0009 0.0001 0.0002 0
Cytoplasm 0.2932 0.486 0.4863 0.5978 0.5748 0.6334 0.6065 0.7192 0.7211 0.7296 0.6791 0.7741 0.2666 0.4152 0.4959 0.753 0.2042 0.5927
Cytoplasmic Foci 0.0217 0.0048 0.0224 0.0075 0.0356 0.0107 0.0033 0.0026 0.0045 0.0115 0.0151 0.0168 0.0647 0.0131 0.0068 0.0036 0.0045 0.0064
Eisosomes 0.0005 0.0001 0.0002 0 0.0007 0 0.0001 0 0.0001 0 0.0001 0 0.0003 0.0001 0.0004 0 0.0002 0
Endoplasmic Reticulum 0.0111 0.0025 0.0049 0.0032 0.0043 0.003 0.0073 0.003 0.0055 0.0086 0.0047 0.0033 0.0052 0.0033 0.0147 0.0026 0.0009 0.0024
Endosome 0.0305 0.0017 0.0075 0.0074 0.0085 0.0048 0.0012 0.0007 0.0028 0.0172 0.0131 0.0028 0.014 0.0109 0.0025 0.0009 0.001 0.0009
Golgi 0.0106 0.0001 0.0031 0.0002 0.0007 0.0002 0.0001 0.0001 0.0003 0.0023 0.0022 0.0001 0.0078 0.002 0.0007 0.0001 0.0003 0.0001
Lipid Particles 0.0109 0.0006 0.0057 0.0002 0.0012 0.0002 0.0003 0.0001 0.0002 0.0006 0.0011 0.0002 0.0148 0.0006 0.0066 0 0.0008 0.0001
Mitochondria 0.004 0.0001 0.0038 0.0005 0.0007 0.0001 0.0001 0.0001 0.0002 0.0009 0.005 0.0001 0.0014 0.0001 0.0005 0.0001 0.0001 0.0001
None 0.494 0.4886 0.4117 0.376 0.3275 0.3433 0.3765 0.2705 0.2465 0.1852 0.1923 0.1942 0.4992 0.5501 0.4571 0.237 0.6889 0.3911
Nuclear Periphery 0.0257 0.0005 0.0011 0.0011 0.0174 0.0004 0.0009 0.0004 0.0014 0.002 0.0017 0.0005 0.0033 0.0003 0.0014 0.0004 0.0004 0.0003
Nucleolus 0.0005 0.0001 0.0002 0.0001 0.0028 0 0.0001 0 0.0001 0.0001 0.0026 0.0002 0.0004 0.0001 0.0002 0 0.0006 0
Nucleus 0.0069 0.0009 0.001 0.0013 0.0108 0.0007 0.0008 0.001 0.0014 0.0019 0.006 0.0019 0.0017 0.0006 0.0008 0.0006 0.0005 0.0009
Peroxisomes 0.0094 0 0.0113 0.0001 0.0006 0.0002 0 0 0.0001 0.0007 0.0017 0.0001 0.0207 0.0001 0.0087 0 0.0003 0.0002
Punctate Nuclear 0.0086 0.0005 0.0038 0.0005 0.01 0.0005 0.0003 0.0003 0.0009 0.0017 0.0046 0.0029 0.0052 0.0004 0.0004 0.0002 0.0004 0.0024
Vacuole 0.0026 0.0009 0.0008 0.0028 0.0013 0.0005 0.0012 0.0006 0.0013 0.002 0.0052 0.0006 0.0019 0.0009 0.001 0.0004 0.0011 0.0002
Vacuole Periphery 0.0014 0.0001 0.0001 0.0006 0.0006 0.0001 0.0001 0 0.0002 0.0005 0.0008 0.0001 0.0006 0.0001 0.0001 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.2549 34.6966 29.4245 23.1638 33.5487 33.2501 37.1699 36.0805 27.9563 32.7213
Translational Efficiency 2.0042 1.3836 1.2686 2.0967 1.4936 1.4069 1.1765 1.4943 1.5174 1.6868

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
186 1121 51 1545 2378 2359 1361 1798 2564 3480 1412 3343

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.79 834.63 1141.15 962.48 743.91 826.44 969.70 1047.70 738.46 829.08 975.89 1008.31
Standard Deviation 121.99 110.56 149.36 126.02 86.36 100.25 122.50 138.72 91.52 103.76 127.64 139.63
Intensity Change Log 2 — 0.319584 0.770863 0.525203 — 0.151782 0.382410 0.494026 — 0.233658 0.579369 0.508870

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000303 0.000572 0.001141 0.001476 0.000134 0.000213 0.000288 0.000685 0.000146 0.000328 0.000319 0.001050
Bud Neck 0.026985 0.016014 0.001860 0.003962 0.013598 0.013832 0.002849 0.005548 0.014569 0.014535 0.002813 0.004815
Bud Site 0.011604 0.022332 0.014044 0.031267 0.007647 0.008926 0.007253 0.032511 0.007934 0.013245 0.007499 0.031936
Cell Periphery 0.000443 0.000215 0.000459 0.000193 0.000093 0.000108 0.000168 0.000093 0.000118 0.000142 0.000179 0.000139
Cytoplasm 0.387247* 0.540623* 0.422086* 0.589838* 0.631443* 0.630952* 0.643678* 0.661692* 0.613728* 0.601854* 0.635675* 0.628484*
Cytoplasmic Foci 0.338753* 0.216471* 0.025376 0.042222 0.139641 0.119281 0.026468 0.030970 0.154085 0.150588 0.026428 0.036170
Eisosomes 0.000442 0.000113 0.000080 0.000048 0.000086 0.000078 0.000037 0.000023 0.000112 0.000089 0.000039 0.000034
Endoplasmic Reticulum 0.001407 0.002812 0.011045 0.001479 0.000905 0.000744 0.002483 0.000609 0.000942 0.001410 0.002792 0.001011
Endosome 0.014718 0.014203 0.005290 0.009286 0.005810 0.005336 0.002541 0.005342 0.006456 0.008192 0.002640 0.007164
Golgi 0.005996 0.003128 0.000330 0.005395 0.001628 0.001618 0.000178 0.001992 0.001945 0.002105 0.000184 0.003565
Lipid Particles 0.013551 0.004957 0.003594 0.001266 0.003124 0.001761 0.001307 0.000697 0.003881 0.002790 0.001390 0.000960
Mitochondria 0.018937 0.002780 0.000325 0.002182 0.002001 0.002485 0.000692 0.001078 0.003229 0.002580 0.000679 0.001588
Mitotic Spindle 0.000109 0.001156 0.000316 0.036923 0.001639 0.002585 0.004542 0.058428 0.001528 0.002125 0.004390 0.048489
None 0.001703 0.002843 0.001378 0.002263 0.002909 0.001983 0.004537 0.004713 0.002821 0.002260 0.004423 0.003581
Nuclear Periphery 0.000260 0.000280 0.001237 0.000481 0.000396 0.000334 0.000841 0.000665 0.000386 0.000317 0.000855 0.000580
Nuclear Periphery Foci 0.000469 0.001723 0.011898 0.002652 0.000293 0.000301 0.005320 0.001355 0.000306 0.000759 0.005558 0.001954
Nucleolus 0.000826 0.000876 0.000317 0.000190 0.000482 0.000593 0.000303 0.000337 0.000507 0.000684 0.000303 0.000269
Nucleus 0.122458 0.069475 0.321920* 0.071295 0.135398* 0.147780* 0.193554* 0.053964 0.134459* 0.122556 0.198190* 0.061974
Peroxisomes 0.012105 0.004988 0.000361 0.002737 0.001710 0.002714 0.000257 0.001244 0.002464 0.003447 0.000261 0.001934
Vacuole 0.039981 0.093117 0.176707 0.191548 0.050422 0.057481 0.102469 0.136817 0.049665 0.068960 0.105150 0.162111
Vacuole Periphery 0.001703 0.001321 0.000237 0.003299 0.000642 0.000895 0.000234 0.001238 0.000719 0.001032 0.000234 0.002191

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.68 -4.24 -2.35 -1.17 2.03 -2.23 -8.70 -2.54 -1.54 0.39 -5.02 -9.30 -3.87 -2.17 -1.37
Bud Neck 2.46 5.45 5.58 9.29 0.34 -0.24 10.13 10.84 10.93 -0.55 0.01 11.24 12.57 14.48 0.05
Bud Site -3.37 0.17 -1.76 1.92 -1.49 -1.31 0.62 -5.12 -4.27 -5.20 -5.12 0.79 -6.54 -2.44 -6.55
Cell Periphery 3.43 0.02 4.95 1.88 5.30 -2.15 -10.17 3.76 4.68 12.58 -1.75 -6.58 1.64 2.78 8.87
Cytoplasm -7.37 1.02 -5.74 3.21 -4.22 0.33 9.60 8.82 8.37 -0.99 2.06 8.47 11.51 10.06 0.86
Cytoplasmic Foci 6.85 18.11 17.85 30.18 -2.84 4.45 32.14 33.62 29.76 2.07 0.86 35.02 35.13 40.23 -1.80
Eisosomes 5.55 5.68 6.64 10.49 3.72 1.63 10.10 16.40 13.16 10.55 3.50 10.69 12.90 15.57 5.52
Endoplasmic Reticulum -2.22 -4.21 -0.10 3.21 4.26 0.91 -11.41 2.44 1.29 15.44 -2.38 -11.83 -0.37 2.61 13.25
Endosome 0.18 2.68 3.30 8.28 1.50 0.94 7.10 6.59 6.89 0.49 -2.66 7.80 5.40 9.24 -1.57
Golgi 2.07 4.15 2.65 1.52 -2.41 0.09 9.75 3.61 2.82 -1.78 -0.50 10.31 1.94 2.19 -3.13
Lipid Particles 4.18 4.67 6.25 4.34 3.20 5.41 6.39 9.66 8.76 8.71 3.00 8.17 10.22 6.11 5.51
Mitochondria 2.41 2.78 2.70 2.11 -2.05 -0.82 4.45 6.12 3.63 -0.02 0.97 4.64 4.61 4.00 -0.67
Mitotic Spindle -4.34 -3.20 -7.43 -6.99 -7.13 -1.01 -4.21 -9.83 -9.36 -7.35 -0.82 -4.29 -11.82 -11.46 -7.91
None -1.16 1.53 -1.21 0.59 -3.26 3.03 -2.61 -2.72 -5.64 -0.30 1.53 -2.69 -1.35 -2.88 1.60
Nuclear Periphery -0.45 -5.62 -11.22 -10.95 3.32 0.33 -6.00 -4.15 -6.55 3.59 0.42 -6.57 -4.03 -8.67 6.12
Nuclear Periphery Foci -4.94 -4.04 -12.69 -3.06 3.36 -0.34 -14.73 -9.61 -9.20 11.92 -5.66 -15.17 -18.77 -9.39 10.72
Nucleolus -0.13 3.88 5.26 3.42 1.16 -0.94 3.49 4.06 2.15 -0.82 -1.65 4.02 7.54 3.91 0.13
Nucleus 4.01 -4.87 2.39 -5.22 6.08 -2.77 -14.00 12.27 13.94 22.30 2.98 -14.76 12.95 10.19 23.50
Peroxisomes 3.13 5.23 4.82 6.33 -1.92 -3.19 12.04 8.70 7.21 -2.25 -3.05 10.86 6.17 8.49 -2.81
Vacuole -9.61 -6.94 -31.95 -27.04 -1.27 -3.26 -19.86 -33.09 -31.23 -12.02 -8.77 -20.90 -47.62 -42.58 -16.12
Vacuole Periphery 0.82 3.11 0.80 0.23 -2.33 -0.91 6.01 2.33 1.74 -2.09 -1.56 6.66 -0.25 0.94 -2.97
Standard name
Human Ortholog
Description Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Add37

Add37


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Add37-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available