Standard name
Human Ortholog
Description Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.2 0.15 0.11 0.15 0.1 0.08 0.11 – 0.06 0.05 0 0.11 0 0 0 0 0.12 0.07 0.17 0.09 0.08 0.18 0.18 0.19
Bud 0.06 0.08 0 0.05 0.06 0.06 0.07 – 0.06 0.08 0.07 0.05 0 0 0 0 0 0 0.1 0.09 0.06 0.18 0.31 0.31
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.06 0.07 0.05 0.06
Bud Site 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.07 0 0 0 – 0 0 0 0 0 0.07 0.05 0.11 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.69 0.77 0.81 0.74 0.79 0.72 0.68 – 0.65 0.57 0.54 0.6 0.92 0.96 0.95 0.84 0.68 0.8 0.49 0.59 0.64 0.32 0.27 0.28
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.05 – 0 0 0 0.05 0 0 0 0 0.07 0 0 0 0 0.06 0.08 0
Endosome 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.17 – 0.31 0.41 0.43 0.28 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0.07 0.09 0 0 0 0 0 0.11 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 67 43 54 58 39 34 59 – 26 18 9 43 25 7 9 3 10 3 61 28 41 57 53 67
Bud 19 22 16 20 23 24 39 – 29 29 24 19 22 5 8 1 1 1 37 29 33 54 93 111
Bud Neck 0 0 1 0 0 0 0 – 0 2 1 0 0 0 0 0 0 0 36 25 32 22 16 20
Bud Site 19 7 6 7 6 13 13 – 8 2 6 4 4 0 0 0 1 0 – – – – – –
Cell Periphery 15 9 26 26 8 16 13 – 20 12 9 17 24 29 23 8 5 3 2 0 3 2 1 1
Cytoplasm 233 225 386 286 296 309 367 – 294 203 177 236 520 421 479 59 57 36 181 189 346 100 80 98
Endoplasmic Reticulum 8 4 12 14 8 22 25 – 16 8 4 18 10 10 10 3 6 1 13 7 12 18 24 14
Endosome 17 7 4 15 5 9 6 – 0 2 3 6 4 0 3 2 2 1 16 11 17 22 4 8
Golgi 1 7 3 1 0 1 0 – 1 0 0 1 0 0 0 1 4 0 1 1 3 2 1 2
Mitochondria 3 4 1 6 13 50 94 – 140 146 140 111 2 5 2 2 3 1 3 3 25 11 10 6
Nucleus 0 0 1 1 1 0 3 – 0 1 4 3 1 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 0 0 0 1 0 0 0 3 0 0 0 1 0
Nucleolus 1 0 0 1 0 1 2 – 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 16 11 1 5 1 0 1 – 5 4 0 2 10 0 4 0 0 0 1 0 0 2 2 2
SpindlePole 14 3 19 19 13 7 10 – 7 6 3 8 4 0 2 1 2 0 6 6 5 5 7 7
Vac/Vac Membrane 2 3 10 12 15 19 10 – 17 24 28 10 1 0 1 2 9 4 1 11 13 6 2 8
Unique Cell Count 340 291 477 389 374 430 541 452 357 327 394 568 437 506 70 84 45 371 319 543 312 303 356
Labelled Cell Count 415 345 540 471 428 505 642 563 458 409 479 628 477 543 82 101 50 371 319 543 312 303 356


Ambiguous, Bud, Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.7 8.1 7.5 7.7 6.4 6.6 – 5.8 5.3 5.1 6.1 7.7 8.1 8.1 12.6 12.0 10.2 7.4 8.0 9.1
Std Deviation (1e-4) 1.0 1.2 0.9 1.1 1.3 1.0 1.1 – 1.0 0.9 0.8 1.1 1.0 1.2 1.3 3.4 2.8 2.3 1.1 1.4 1.7
Intensity Change (Log2) – – – -0.1 -0.07 -0.33 -0.31 – -0.47 -0.6 -0.67 -0.42 -0.07 -0.0 0.01 0.63 0.57 0.34 -0.13 -0.02 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -0.4 -1.7 -0.2 – -3.0 -3.2 -4.4 -0.2 -4.2 -5.9 -6.1 -1.8 0.2 0
Bud 1.3 1.9 1.6 2.7 – 2.2 3.0 2.6 1.1 0.4 -2.2 -1.8 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0.7 0.4 1.9 1.3 – 0.6 0 0 0 0 0 0 0 0 0
Cell Periphery 0.8 -2.4 -1.2 -2.5 – -0.7 -1.4 -1.8 -0.8 -0.9 0.8 -0.7 0 0 0
Cytoplasm -2.6 -0.6 -3.2 -4.7 – -5.5 -7.5 -8.1 -6.8 5.0 7.2 6.6 0.7 -2.7 -0.2
Endoplasmic Reticulum 0.9 -0.4 2.1 1.8 – 0.9 -0.3 -1.3 1.7 -0.8 -0.2 -0.6 0 0 0
Endosome 3.0 0 1.6 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.7 7.5 9.4 – 13.1 15.3 15.6 12.3 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 2.4 0 0 0 0 0
SpindlePole 0.6 -0.4 -2.1 -2.0 – -2.2 -1.9 -2.6 -1.7 -3.6 -4.2 -3.9 0 0 0
Vacuole 0.9 1.6 2.0 -0.3 – 1.5 3.3 4.2 0.4 -3.0 0 -2.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9328 3.206 3.0408 2.6499 2.9721 3.1384 3.8796 4.6821 4.1493 3.7489 3.7127 4.4987 3.0181 3.7908 3.1073 3.4684 2.7193 3.331
Actin 0.0553 0.0122 0.0503 0.0282 0.0665 0.0572 0.0469 0.0281 0.0501 0.0172 0.0452 0.029 0.0897 0.0223 0.0604 0.024 0.0147 0.0263
Bud 0.0163 0.0299 0.0923 0.0088 0.0188 0.007 0.018 0.0093 0.0538 0.0164 0.003 0.0036 0.0143 0.0204 0.0524 0.0163 0.0026 0.0041
Bud Neck 0.0144 0.0029 0.0019 0.0017 0.0485 0.2394 0.0297 0.0049 0.0115 0.0289 0.0485 0.3228 0.0124 0.0043 0.0083 0.0023 0.0431 0.2994
Bud Periphery 0.0166 0.0185 0.0895 0.0143 0.0084 0.0062 0.0166 0.0066 0.0507 0.0175 0.0029 0.0054 0.0157 0.0101 0.0476 0.0302 0.0027 0.0053
Bud Site 0.0733 0.1619 0.0483 0.0075 0.0125 0.0048 0.1338 0.2101 0.0678 0.022 0.0257 0.0065 0.1104 0.1721 0.0321 0.0056 0.0015 0.0038
Cell Periphery 0.0024 0.0017 0.0036 0.0007 0.0007 0.0013 0.0027 0.0013 0.0017 0.0016 0.0007 0.0008 0.0027 0.0011 0.0011 0.0005 0.0005 0.0007
Cytoplasm 0.5198 0.6515 0.5298 0.7277 0.636 0.4262 0.5611 0.6279 0.6342 0.6965 0.6583 0.5096 0.4384 0.6875 0.6751 0.7175 0.656 0.4919
Cytoplasmic Foci 0.0908 0.0422 0.0599 0.0502 0.0432 0.1477 0.0667 0.058 0.0463 0.0372 0.0378 0.0736 0.1529 0.0357 0.0361 0.0532 0.0537 0.0872
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0145 0.0039 0.0029 0.0052 0.0097 0.0026 0.0074 0.0027 0.0039 0.0121 0.0051 0.0028 0.0137 0.0016 0.0062 0.0028 0.0052 0.0034
Endosome 0.0688 0.0184 0.0364 0.0578 0.0855 0.0273 0.0315 0.0127 0.0328 0.0551 0.0433 0.0198 0.044 0.0067 0.0284 0.0613 0.0723 0.02
Golgi 0.0191 0.0046 0.0147 0.0108 0.0191 0.0164 0.0157 0.0067 0.0084 0.0155 0.0158 0.008 0.0149 0.0058 0.0111 0.0153 0.0212 0.0079
Lipid Particles 0.0136 0.0005 0.0057 0.0009 0.0038 0.0083 0.01 0.0005 0.0035 0.002 0.0115 0.0016 0.0101 0.0005 0.0025 0.0056 0.0081 0.0016
Mitochondria 0.0062 0.0021 0.0105 0.0039 0.0082 0.0156 0.0026 0.0078 0.0032 0.0113 0.0057 0.0017 0.0127 0.0006 0.0102 0.0102 0.0638 0.0028
None 0.0345 0.0339 0.0262 0.0628 0.0081 0.0073 0.0151 0.0047 0.0058 0.0123 0.026 0.0045 0.0135 0.0176 0.0096 0.0261 0.0328 0.0263
Nuclear Periphery 0.0129 0.0008 0.0011 0.0012 0.0143 0.0006 0.0056 0.0004 0.0011 0.0183 0.0064 0.0008 0.0149 0.0003 0.0028 0.0033 0.0007 0.0063
Nucleolus 0.0015 0.0004 0.0015 0.0002 0.0009 0.0003 0.0013 0.0001 0.0008 0.0007 0.0017 0.0001 0.0009 0.0002 0.0004 0.0005 0.0001 0.0003
Nucleus 0.0053 0.0021 0.0028 0.0016 0.0029 0.0009 0.0043 0.0011 0.0022 0.0069 0.0047 0.0007 0.0069 0.0009 0.0019 0.0018 0.0008 0.0017
Peroxisomes 0.0174 0.0041 0.0115 0.0035 0.002 0.0189 0.0135 0.0124 0.0061 0.0099 0.0264 0.0051 0.0135 0.0094 0.0055 0.0086 0.0136 0.0066
Punctate Nuclear 0.0044 0.0011 0.0025 0.0016 0.0037 0.0089 0.0103 0.0012 0.0073 0.0032 0.0265 0.0012 0.0114 0.0011 0.0022 0.003 0.0007 0.0026
Vacuole 0.0099 0.0064 0.0065 0.0096 0.0042 0.0022 0.0061 0.003 0.0076 0.0122 0.0031 0.0017 0.0049 0.0016 0.0045 0.0077 0.0042 0.0013
Vacuole Periphery 0.0025 0.0008 0.0017 0.002 0.0025 0.0009 0.001 0.0003 0.001 0.0029 0.0012 0.0004 0.0016 0.0002 0.0012 0.0038 0.0015 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.988 5.4723 8.4597 13.52 14.5508 7.6477 12.334 14.782 15.5134 16.8963
Translational Efficiency 1.5772 3.6677 1.7814 1.2187 1.2015 2.8079 1.588 1.2394 1.2392 1.3121

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
207 891 2337 1342 1495 1696 1770 43 1702 2587 4107 1385

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 822.58 854.81 1229.46 1143.67 731.44 818.16 1080.86 1170.65 742.52 830.78 1165.42 1144.51
Standard Deviation 92.13 193.85 157.80 165.37 146.31 139.23 144.33 186.71 143.95 161.10 169.01 166.14
Intensity Change Log 2 — 0.055448 0.579797 0.475443 — 0.161643 0.563368 0.678498 — 0.106407 0.572088 0.574583

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.022863 0.006007 0.014575 0.101116* 0.007222 0.008686 0.020562 0.048301 0.009124 0.007764 0.017155 0.099477
Bud Neck 0.047971 0.021606 0.018731 0.009664 0.030815 0.026027 0.028258 0.007764 0.032901 0.024504 0.022836 0.009605
Bud Site 0.257294* 0.210848* 0.151469* 0.270297* 0.162940* 0.188134* 0.263397* 0.261981* 0.174416* 0.195957* 0.199707* 0.270039*
Cell Periphery 0.003106 0.000761 0.000134 0.001589 0.000521 0.000413 0.000246 0.004370 0.000835 0.000533 0.000183 0.001675
Cytoplasm 0.397369* 0.379187* 0.352521* 0.448915* 0.382191* 0.457570* 0.453602* 0.349968* 0.384037* 0.430574* 0.396084* 0.445843*
Cytoplasmic Foci 0.160096 0.215323* 0.148799 0.062998 0.224752* 0.181248* 0.072092 0.137383 0.216888* 0.192984* 0.115741 0.065308
Eisosomes 0.004129 0.000216 0.000066 0.000293 0.000358 0.000381 0.000114 0.000583 0.000817 0.000324 0.000087 0.000302
Endoplasmic Reticulum 0.001545 0.001671 0.002928 0.000540 0.004512 0.000890 0.000653 0.001414 0.004151 0.001159 0.001947 0.000567
Endosome 0.019948 0.049542 0.051381 0.008096 0.045977 0.033434 0.010292 0.027380 0.042811 0.038982 0.033673 0.008695
Golgi 0.037061 0.025394 0.009456 0.006420 0.024730 0.026119 0.001770 0.012280 0.026230 0.025869 0.006143 0.006602
Lipid Particles 0.003449 0.004513 0.006420 0.003087 0.009447 0.002746 0.001803 0.006069 0.008717 0.003354 0.004430 0.003180
Mitochondria 0.004426 0.002020 0.001257 0.000973 0.004534 0.005905 0.000264 0.031206 0.004521 0.004567 0.000829 0.001912
Mitotic Spindle 0.000274 0.002460 0.018813 0.014958 0.001414 0.002227 0.006791 0.001302 0.001275 0.002308 0.013632 0.014534
None 0.000254 0.006581 0.000166 0.000063 0.013849 0.000780 0.000301 0.000038 0.012195 0.002778 0.000224 0.000062
Nuclear Periphery 0.000015 0.000257 0.000239 0.000064 0.000409 0.000069 0.000055 0.000023 0.000361 0.000133 0.000160 0.000062
Nuclear Periphery Foci 0.000182 0.001663 0.001588 0.000445 0.002397 0.000304 0.000542 0.001044 0.002127 0.000772 0.001137 0.000464
Nucleolus 0.000057 0.002061 0.000221 0.000057 0.002069 0.000393 0.000075 0.000025 0.001824 0.000968 0.000158 0.000056
Nucleus 0.002518 0.013724 0.007114 0.001845 0.019134 0.004336 0.005379 0.001501 0.017113 0.007570 0.006366 0.001834
Peroxisomes 0.016590 0.018775 0.042219 0.025323 0.017455 0.020587 0.025579 0.033400 0.017350 0.019963 0.035048 0.025574
Vacuole 0.018480 0.033643 0.170166 0.040658 0.041642 0.036013 0.107709 0.073490 0.038825 0.035197 0.143248 0.041678
Vacuole Periphery 0.002374 0.003748 0.001738 0.002598 0.003634 0.003739 0.000517 0.000477 0.003480 0.003742 0.001212 0.002532

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.14 1.86 -8.12 -13.54 -12.36 -0.99 -4.75 -2.20 -2.11 -1.51 0.99 -3.76 -13.06 -13.43 -12.09
Bud Neck 3.30 3.96 5.04 6.06 8.28 1.72 2.66 8.92 8.22 7.68 3.34 5.67 10.96 10.94 11.40
Bud Site 2.18 6.05 0.67 -2.89 -12.72 -2.95 -6.76 -1.76 -1.15 -0.25 -2.73 0.21 -7.29 -5.43 -8.54
Cell Periphery 4.08 5.33 2.49 -1.32 -2.87 1.45 5.62 -1.04 -1.08 -1.14 2.97 7.99 -1.36 -2.08 -2.96
Cytoplasm 0.83 1.78 -3.29 -6.26 -9.30 -7.09 -8.10 0.18 1.82 2.04 -5.09 -2.88 -6.97 -2.88 -5.34
Cytoplasmic Foci -4.37 1.52 8.55 19.62 16.75 5.98 25.15 3.31 1.69 -2.38 3.79 19.01 25.45 24.60 11.48
Eisosomes 3.92 4.07 3.87 -0.78 -4.96 -0.22 5.37 -0.43 -0.35 -1.29 3.42 5.61 4.05 0.86 -4.67
Endoplasmic Reticulum -0.24 -1.62 2.43 3.70 4.34 4.11 4.35 2.82 -0.47 -0.83 3.85 2.99 4.71 3.71 3.95
Endosome -6.26 -6.78 3.58 12.32 18.65 4.29 15.37 2.06 0.90 -1.35 1.47 5.27 15.60 16.53 15.64
Golgi 2.16 5.58 6.03 10.05 2.36 -0.76 17.60 1.74 1.91 -1.05 0.21 15.38 13.06 14.53 -0.45
Lipid Particles -0.98 -4.43 0.20 1.11 4.45 5.65 6.70 1.32 -0.84 -1.19 5.04 4.36 4.89 0.13 1.72
Mitochondria 2.42 3.51 3.53 2.41 0.65 -1.23 6.33 -0.90 -0.84 -1.09 -0.08 6.21 2.85 2.92 -1.08
Mitotic Spindle -3.57 -11.23 -5.86 -4.79 1.91 -1.43 -4.23 0.20 1.27 4.05 -2.26 -11.20 -5.41 -4.93 0.12
None -3.47 0.51 1.23 3.58 5.54 6.26 6.54 6.66 3.63 3.44 4.85 6.56 6.65 4.24 4.75
Nuclear Periphery -1.92 -8.92 -2.26 1.43 5.36 3.19 3.19 3.60 1.42 4.17 2.22 1.80 3.06 1.19 4.07
Nuclear Periphery Foci -2.90 -8.54 -4.57 2.22 6.72 3.69 3.16 1.44 -3.15 -2.33 2.54 1.74 3.14 1.16 6.10
Nucleolus -4.21 -11.26 -0.15 4.21 11.52 5.70 7.10 7.28 4.04 5.49 2.80 6.69 7.15 5.19 8.73
Nucleus -4.41 -11.99 -0.04 4.44 15.39 7.55 6.29 8.27 1.76 4.81 5.02 5.47 8.93 5.13 16.02
Peroxisomes -0.62 -5.52 -1.81 -1.75 5.59 -1.70 -2.58 -1.49 -1.08 -0.82 -1.66 -7.78 -2.95 -1.67 3.70
Vacuole -4.38 -32.49 -10.56 -7.53 21.51 2.04 -18.62 -2.56 -2.81 1.62 1.55 -33.78 -6.95 -8.22 20.85
Vacuole Periphery -2.36 2.38 0.21 1.77 -0.90 -0.27 12.89 9.77 7.61 0.14 -0.76 11.60 1.83 2.10 -1.53
Standard name
Human Ortholog
Description Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Gyl1

Gyl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gyl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available