Standard name | |
---|---|
Human Ortholog | |
Description | Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 2 | 2 | 0 | 0 | 3 | 7 | – | 0 | 1 | 2 | 4 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud | – | 0 | 2 | 0 | 0 | 4 | 7 | 12 | – | 14 | 12 | 9 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 1 | 1 | 4 | 7 | 16 | 14 | 9 | – | 21 | 22 | 13 | 6 | 16 | 9 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 70 | 81 | 128 | 240 | 352 | 400 | 511 | – | 469 | 429 | 599 | 97 | 93 | 133 | 185 | 137 | 119 | 4 | 11 | 10 | 6 | 9 | 22 |
Endoplasmic Reticulum | – | 49 | 81 | 62 | 111 | 102 | 89 | 63 | – | 63 | 34 | 57 | 123 | 219 | 204 | 2 | 1 | 3 | 8 | 86 | 140 | 111 | 171 | 207 |
Endosome | – | 0 | 4 | 1 | 0 | 0 | 1 | 2 | – | 2 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 5 |
Golgi | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 4 | 0 | 0 | 0 | 1 | 1 | 2 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 1 | 2 | 4 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | – | 1 | 15 | 10 | 14 | 15 | 40 | 38 | – | 59 | 43 | 42 | 4 | 12 | 16 | 96 | 87 | 72 | 10 | 14 | 17 | 43 | 48 | 55 |
Unique Cell Count | 110 | 158 | 193 | 354 | 456 | 515 | 600 | 575 | 497 | 689 | 216 | 301 | 342 | 274 | 222 | 190 | 28 | 118 | 177 | 173 | 244 | 304 | ||
Labelled Cell Count | 121 | 188 | 207 | 373 | 490 | 558 | 643 | 630 | 544 | 727 | 235 | 348 | 369 | 294 | 234 | 205 | 28 | 118 | 177 | 173 | 244 | 304 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 24.1 | 14.7 | 15.1 | 15.0 | 12.8 | 11.8 | 11.8 | – | 11.3 | 10.8 | 11.4 | 20.4 | 19.5 | 19.0 | 24.3 | 26.7 | 25.2 | 21.3 | 21.7 | 22.1 |
Std Deviation (1e-4) | – | 4.8 | 2.6 | 2.9 | 2.3 | 2.3 | 2.4 | 2.3 | – | 2.5 | 2.4 | 2.1 | 3.8 | 3.3 | 2.9 | 5.9 | 6.2 | 6.9 | 4.7 | 4.3 | 3.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.03 | -0.19 | -0.31 | -0.31 | – | -0.38 | -0.44 | -0.36 | 0.48 | 0.41 | 0.37 | 0.73 | 0.86 | 0.78 | 0.54 | 0.56 | 0.59 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.3 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.9 | 3.6 | 6.2 | 6.4 | 9.2 | – | 7.8 | 9.2 | 10.2 | -1.2 | -4.3 | -2.6 | 3.3 | 2.0 | 2.1 |
Endoplasmic Reticulum | -3.6 | -4.3 | -6.8 | -8.6 | -11.6 | – | -11.3 | -12.8 | -13.2 | 1.1 | 4.6 | 1.8 | -12.8 | -11.9 | -10.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.6 | -2.5 | -3.1 | -0.7 | -1.4 | – | 0.3 | -0.3 | -1.5 | -3.3 | -2.4 | -2.1 | 5.9 | 6.4 | 6.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.6691 | 23.0349 | 21.8397 | 19.253 | 21.0207 | 21.1854 | 17.9595 | 20.677 | 19.9842 | 18.5838 | 15.8535 | 19.5439 | 21.024 | 23.7491 | 22.109 | 21.5792 | 19.699 | 21.0857 |
Actin | 0.0002 | 0.0018 | 0.0039 | 0.0124 | 0.0002 | 0.0001 | 0.0029 | 0.0006 | 0.0028 | 0.0001 | 0.0029 | 0.0001 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0001 | 0.0004 | 0.0006 | 0.001 | 0.0015 | 0.0007 | 0.0002 | 0.002 | 0.0019 | 0.0003 | 0.014 | 0.0007 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0019 | 0.0006 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0022 | 0.0016 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0002 |
Bud Site | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0026 | 0.0011 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0043 | 0.0121 | 0.0092 | 0.012 | 0.0131 | 0.003 | 0.0034 | 0.0057 | 0.0044 | 0.0039 | 0.0087 | 0.0036 | 0.0048 | 0.0128 | 0.0065 | 0.0069 | 0.0153 | 0.0035 |
Cytoplasm | 0.0322 | 0.0625 | 0.0552 | 0.0488 | 0.0663 | 0.0344 | 0.0454 | 0.0615 | 0.0354 | 0.0142 | 0.0695 | 0.0236 | 0.0366 | 0.0451 | 0.0354 | 0.034 | 0.0543 | 0.0272 |
Cytoplasmic Foci | 0.0003 | 0.0006 | 0.0011 | 0.0033 | 0.0016 | 0.0003 | 0.0073 | 0.0037 | 0.0032 | 0.0009 | 0.0044 | 0.0048 | 0.0043 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0.0003 |
Eisosomes | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.8533 | 0.8429 | 0.8141 | 0.7206 | 0.7998 | 0.8431 | 0.7778 | 0.8109 | 0.8439 | 0.7232 | 0.6789 | 0.815 | 0.7621 | 0.8287 | 0.8294 | 0.7689 | 0.6095 | 0.8364 |
Endosome | 0.0057 | 0.0026 | 0.0131 | 0.0195 | 0.0136 | 0.0034 | 0.0283 | 0.0161 | 0.0169 | 0.0264 | 0.0389 | 0.0186 | 0.0048 | 0.0018 | 0.006 | 0.0048 | 0.0143 | 0.0054 |
Golgi | 0.0003 | 0.0005 | 0.0013 | 0.0066 | 0.0007 | 0.0002 | 0.0028 | 0.0092 | 0.0014 | 0.0008 | 0.0016 | 0.0016 | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0001 |
Lipid Particles | 0.0046 | 0.0133 | 0.017 | 0.0202 | 0.0087 | 0.0023 | 0.0173 | 0.0088 | 0.0183 | 0.0029 | 0.0045 | 0.005 | 0.003 | 0.008 | 0.0083 | 0.0023 | 0.0063 | 0.0016 |
Mitochondria | 0.0007 | 0 | 0.0011 | 0.0005 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0012 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 |
None | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0034 | 0.0001 | 0.0008 | 0.0001 | 0.0288 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Nuclear Periphery | 0.0573 | 0.019 | 0.0221 | 0.0301 | 0.0332 | 0.0489 | 0.0543 | 0.0151 | 0.0196 | 0.0244 | 0.0237 | 0.0329 | 0.0872 | 0.0225 | 0.0388 | 0.0419 | 0.0583 | 0.0399 |
Nucleolus | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Nucleus | 0.0049 | 0.0014 | 0.0013 | 0.0018 | 0.0036 | 0.0059 | 0.0035 | 0.0022 | 0.0014 | 0.0013 | 0.0043 | 0.0021 | 0.0041 | 0.0026 | 0.0017 | 0.0052 | 0.003 | 0.003 |
Peroxisomes | 0 | 0 | 0.0001 | 0.0009 | 0 | 0 | 0.0034 | 0 | 0.0004 | 0 | 0.0001 | 0.0006 | 0.0014 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0007 | 0.0011 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0329 | 0.0417 | 0.0578 | 0.1168 | 0.0548 | 0.0549 | 0.0442 | 0.0566 | 0.0403 | 0.1894 | 0.1111 | 0.0842 | 0.0834 | 0.0755 | 0.0674 | 0.1292 | 0.2274 | 0.076 |
Vacuole Periphery | 0.0025 | 0.0007 | 0.0017 | 0.0041 | 0.0018 | 0.0025 | 0.0032 | 0.0015 | 0.0033 | 0.0112 | 0.0057 | 0.0052 | 0.0055 | 0.0017 | 0.0042 | 0.0054 | 0.0077 | 0.0055 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.1166 | 56.2912 | 36.0746 | 56.7658 | 57.5214 | 47.2013 | 61.12 | 55.0025 | 52.8645 | 75.9341 |
Translational Efficiency | 1.3527 | 1.332 | 1.5159 | 1.2215 | 1.3217 | 2.2104 | 1.45 | 1.3501 | 1.5733 | 1.2985 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1806 | 1600 | 2480 | 1090 | 2621 | 482 | 3055 | 1535 | 4427 | 2082 | 5535 | 2625 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1384.49 | 1636.22 | 2199.34 | 2315.93 | 1528.93 | 1739.92 | 2189.93 | 2341.47 | 1470.01 | 1660.23 | 2194.15 | 2330.86 |
Standard Deviation | 229.78 | 389.31 | 363.98 | 453.27 | 265.50 | 428.99 | 321.98 | 480.48 | 261.37 | 401.24 | 341.47 | 469.54 |
Intensity Change Log 2 | — | 0.241012 | 0.667716 | 0.742237 | — | 0.186499 | 0.518362 | 0.614892 | — | 0.212661 | 0.591267 | 0.676811 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000136 | 0.000321 | 0.000055 | 0.001046 | 0.000106 | 0.000343 | 0.000093 | 0.000579 | 0.000118 | 0.000326 | 0.000076 | 0.000773 |
Bud Neck | 0.000213 | 0.000700 | 0.001189 | 0.003129 | 0.000489 | 0.000520 | 0.000274 | 0.001558 | 0.000377 | 0.000658 | 0.000684 | 0.002210 |
Bud Site | 0.001525 | 0.004218 | 0.001417 | 0.009732 | 0.002378 | 0.002312 | 0.001131 | 0.005740 | 0.002030 | 0.003777 | 0.001259 | 0.007398 |
Cell Periphery | 0.005498 | 0.007642 | 0.004415 | 0.048572 | 0.003957 | 0.007361 | 0.010383 | 0.037371 | 0.004586 | 0.007577 | 0.007709 | 0.042022 |
Cytoplasm | 0.015188 | 0.038301 | 0.039758 | 0.016956 | 0.035541 | 0.008456 | 0.016358 | 0.017334 | 0.027238 | 0.031392 | 0.026843 | 0.017177 |
Cytoplasmic Foci | 0.003579 | 0.008857 | 0.003099 | 0.001541 | 0.006779 | 0.003627 | 0.000465 | 0.001882 | 0.005474 | 0.007646 | 0.001645 | 0.001741 |
Eisosomes | 0.000015 | 0.000023 | 0.000008 | 0.000199 | 0.000017 | 0.000021 | 0.000025 | 0.000124 | 0.000016 | 0.000022 | 0.000018 | 0.000155 |
Endoplasmic Reticulum | 0.889848* | 0.747963* | 0.469264* | 0.701716* | 0.827147* | 0.892517* | 0.923237* | 0.790003* | 0.852726* | 0.781428* | 0.719831* | 0.753343* |
Endosome | 0.007967 | 0.023838 | 0.023956 | 0.014616 | 0.012122 | 0.014686 | 0.004036 | 0.012080 | 0.010427 | 0.021719 | 0.012961 | 0.013133 |
Golgi | 0.001862 | 0.010750 | 0.008816 | 0.007973 | 0.002916 | 0.006961 | 0.000924 | 0.008074 | 0.002486 | 0.009873 | 0.004460 | 0.008032 |
Lipid Particles | 0.002385 | 0.008837 | 0.013996 | 0.065562 | 0.003686 | 0.007694 | 0.005054 | 0.046856 | 0.003155 | 0.008572 | 0.009060 | 0.054623 |
Mitochondria | 0.000316 | 0.001324 | 0.000510 | 0.000802 | 0.000636 | 0.001263 | 0.000998 | 0.000729 | 0.000505 | 0.001310 | 0.000779 | 0.000759 |
Mitotic Spindle | 0.000041 | 0.000276 | 0.000258 | 0.000596 | 0.000033 | 0.000124 | 0.000089 | 0.000420 | 0.000037 | 0.000240 | 0.000165 | 0.000493 |
None | 0.000358 | 0.001808 | 0.000774 | 0.001480 | 0.001119 | 0.000188 | 0.000917 | 0.001132 | 0.000809 | 0.001433 | 0.000853 | 0.001276 |
Nuclear Periphery | 0.000385 | 0.001466 | 0.009073 | 0.002789 | 0.000839 | 0.000560 | 0.000487 | 0.001691 | 0.000654 | 0.001256 | 0.004334 | 0.002147 |
Nuclear Periphery Foci | 0.002473 | 0.019240 | 0.056637 | 0.019344 | 0.004667 | 0.006718 | 0.002119 | 0.009199 | 0.003772 | 0.016341 | 0.026546 | 0.013411 |
Nucleolus | 0.000060 | 0.000257 | 0.000120 | 0.000036 | 0.000056 | 0.000919 | 0.000022 | 0.000033 | 0.000058 | 0.000410 | 0.000066 | 0.000034 |
Nucleus | 0.000230 | 0.000646 | 0.000744 | 0.000847 | 0.000494 | 0.001453 | 0.000184 | 0.000518 | 0.000386 | 0.000833 | 0.000435 | 0.000654 |
Peroxisomes | 0.000185 | 0.000232 | 0.000053 | 0.000190 | 0.000249 | 0.000206 | 0.000580 | 0.000402 | 0.000223 | 0.000226 | 0.000344 | 0.000314 |
Vacuole | 0.063952 | 0.105654 | 0.335818* | 0.098290 | 0.089740 | 0.039902 | 0.031777 | 0.060197 | 0.079220 | 0.090432 | 0.168005 | 0.076014 |
Vacuole Periphery | 0.003784 | 0.017646 | 0.030042 | 0.004586 | 0.007028 | 0.004169 | 0.000849 | 0.004078 | 0.005705 | 0.014526 | 0.013929 | 0.004289 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.42 | 1.82 | -4.02 | -3.22 | -4.32 | -1.48 | 0.19 | -3.45 | -1.49 | -3.46 | -2.01 | 1.42 | -5.29 | -3.43 | -5.54 |
Bud Neck | -5.10 | -9.61 | -7.69 | -6.39 | -5.07 | -0.38 | 1.78 | -4.70 | -3.15 | -5.72 | -2.71 | -4.23 | -8.82 | -7.20 | -7.40 |
Bud Site | -4.58 | 0.18 | -6.14 | -4.49 | -6.25 | 0.09 | 2.05 | -3.83 | -3.25 | -5.02 | -3.35 | 1.99 | -7.13 | -5.05 | -8.01 |
Cell Periphery | -2.70 | 1.86 | -13.67 | -12.96 | -14.20 | -1.87 | -8.95 | -15.29 | -10.73 | -11.91 | -4.46 | -6.58 | -20.45 | -18.06 | -18.55 |
Cytoplasm | -8.07 | -10.69 | -1.45 | 5.91 | 7.53 | 9.51 | 9.30 | 6.79 | -3.68 | -1.10 | -1.82 | -0.12 | 4.74 | 5.22 | 5.11 |
Cytoplasmic Foci | -5.79 | 1.02 | 3.52 | 8.75 | 4.34 | 2.74 | 9.14 | 6.40 | 1.75 | -3.70 | -2.82 | 8.36 | 7.30 | 8.62 | -0.62 |
Eisosomes | -2.13 | 2.53 | -5.95 | -5.71 | -6.19 | -1.26 | -2.40 | -4.96 | -4.75 | -4.57 | -2.41 | -0.60 | -7.71 | -7.37 | -7.64 |
Endoplasmic Reticulum | 15.48 | 49.24* | 17.46 | 4.41 | -17.91 | -5.69 | -15.92 | 4.78 | 8.52 | 16.91 | 9.55 | 22.85 | 15.02 | 3.96 | -3.67 |
Endosome | -13.28 | -18.08 | -6.69 | 6.24 | 7.70 | -1.49 | 12.13 | -0.17 | 1.39 | -11.87 | -11.36 | -4.57 | -4.47 | 7.92 | -0.69 |
Golgi | -8.07 | -10.11 | -7.46 | 2.13 | 0.79 | -1.97 | 5.37 | -4.86 | -0.50 | -7.11 | -7.64 | -5.23 | -7.84 | 1.60 | -5.01 |
Lipid Particles | -6.24 | -9.77 | -13.93 | -12.32 | -11.10 | -1.65 | -1.86 | -13.77 | -10.10 | -13.17 | -5.68 | -8.64 | -19.55 | -16.69 | -16.97 |
Mitochondria | -2.54 | -3.03 | -2.37 | 1.02 | -1.53 | -0.85 | -0.85 | -0.84 | 0.69 | 0.56 | -2.29 | -1.16 | -2.32 | 1.40 | -0.08 |
Mitotic Spindle | -3.59 | -5.47 | -3.71 | -2.38 | -2.53 | -1.99 | -0.84 | -3.04 | -2.24 | -2.31 | -4.07 | -3.11 | -4.78 | -2.71 | -3.38 |
None | -4.42 | -2.85 | -1.98 | 0.49 | -1.29 | 3.70 | 0.71 | -0.04 | -1.93 | -0.44 | -2.23 | -0.19 | -1.21 | 0.33 | -1.16 |
Nuclear Periphery | -5.02 | -16.49 | -12.87 | -4.81 | 11.35 | 1.62 | 2.21 | -3.88 | -6.19 | -7.24 | -3.22 | -14.30 | -9.92 | -4.42 | 8.19 |
Nuclear Periphery Foci | -9.88 | -22.59 | -8.97 | -0.57 | 11.95 | -1.45 | 4.80 | -4.25 | -1.74 | -6.54 | -9.30 | -19.64 | -9.57 | 1.22 | 8.39 |
Nucleolus | -1.47 | -1.63 | 0.73 | 1.70 | 4.92 | -1.34 | 6.75 | 4.14 | 1.37 | -2.25 | -1.90 | -0.50 | 1.71 | 2.01 | 3.85 |
Nucleus | -3.69 | -4.87 | -3.99 | -1.99 | -1.43 | -1.12 | 4.44 | -1.42 | 0.98 | -4.39 | -1.91 | -1.19 | -4.12 | 0.13 | -3.19 |
Peroxisomes | -0.63 | 6.51 | -0.08 | 0.52 | -3.42 | 0.27 | -2.68 | -1.92 | -1.35 | 1.35 | -0.08 | -1.72 | -1.79 | -1.11 | 0.40 |
Vacuole | -7.29 | -39.18 | -5.94 | 0.42 | 28.61 | 7.60 | 14.41 | 5.58 | -3.50 | -7.81 | -2.52 | -20.80 | -0.06 | 2.31 | 19.29 |
Vacuole Periphery | -10.56 | -19.98 | -1.27 | 9.61 | 18.71* | 2.89 | 11.55 | 4.18 | 0.10 | -6.98 | -8.45 | -11.78 | 2.76 | 9.80 | 13.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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