Standard name
Human Ortholog
Description Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.15 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.06 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0 0.14 0.09 0.06 0.11 0.06 0 0.06 0 – – – 0.1 0.15 0.09 0 0 0 0 0 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0.08 0.06 0
Mitochondria 0.3 0.09 0.17 0.17 0 0.16 0.15 0.21 0.38 0.37 0.59 0.36 – – – 0.08 0.07 0 0 0 0 0.08 0.06 0
Nucleus 0.81 0.82 0.94 0.89 0.78 0.76 0.79 0.76 0.73 0.78 0.7 0.85 – – – 0.69 0.59 0.74 0.74 0.76 0.69 0.62 0.38 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0.13 0 0 0.07 0.08 0.09 0 0 0 0.05 0 – – – 0.16 0.19 0.15 0.12 0.11 0.16 0.16 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 1 0 0 0 0 3 0
Bud 0 0 0 0 0 1 1 1 0 0 1 0 – – – 2 1 0 1 5 0 0 0 1
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 2 4 2 2 8 3 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 6 13 1 2 23 17 17 38 15 6 10 6 – – – 25 28 18 0 5 0 0 0 2
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 1 0 0 0 – – – 16 9 9 0 0 1 0 0 0
Endosome 0 3 0 0 1 2 0 5 1 0 1 1 – – – 14 8 6 1 2 1 0 0 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 – – – 1 0 2 0 4 0 0 1 0
Mitochondria 49 22 3 14 5 30 45 69 89 75 103 69 – – – 20 12 7 6 2 1 0 1 0
Nucleus 135 209 17 71 128 147 232 252 169 160 121 162 – – – 180 108 148 138 218 51 8 8 16
Nuclear Periphery 0 0 0 0 0 1 0 3 2 0 3 1 – – – 1 1 0 4 1 0 0 0 0
Nucleolus 1 0 0 1 0 1 1 1 1 0 2 3 – – – 2 0 1 7 5 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 1 3 4 6 9 2 3 1 0 – – – 4 1 2 2 6 1 0 1 0
Vac/Vac Membrane 7 32 0 3 11 15 25 9 5 6 9 3 – – – 43 34 30 21 30 11 2 1 2
Unique Cell Count 166 255 18 80 165 193 294 332 233 204 174 190 261 182 201 188 287 74 13 21 28
Labelled Cell Count 199 281 21 93 171 218 330 393 287 252 259 248 309 202 224 188 287 74 13 21 28


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.0 5.0 4.9 6.6 5.0 5.0 5.3 4.5 4.4 4.2 4.3 – – – 6.6 6.6 6.6 – – –
Std Deviation (1e-4) 0.5 1.2 0.7 1.3 2.8 1.4 1.3 1.8 1.2 0.9 1.4 0.8 – – – 1.3 1.6 1.8 – – –
Intensity Change (Log2) – – – -0.02 0.42 0.01 0.01 0.1 -0.15 -0.19 -0.23 -0.2 – – – 0.42 0.41 0.4 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleus -0.7 -1.7 -1.8 -1.6 -1.8 -2.0 -1.6 -2.2 -1.1 – – – -2.3 -2.9 -2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1205 0.8859 0.8455 1.0709 0.579 0.6035 0.787 1.05 0.7635 1.1006 2.0759 0.8288 0.4081 0.4293 0.206 0.5344 0.1766 0.1305
Actin 0.0098 0.0001 0.0012 0.0043 0.0023 0.0004 0.0121 0.0001 0.0125 0.0331 0.0165 0.001 0.0142 0 0.0141 0.0033 0.0415 0.001
Bud 0.0006 0.0009 0.0004 0.0024 0.0001 0.0001 0.0004 0 0.0002 0.0013 0.0025 0.0001 0.0004 0.0001 0.0004 0.0003 0.0005 0.0001
Bud Neck 0.0033 0.0001 0.0004 0.0036 0.0002 0.001 0.0016 0.0001 0.0011 0.0013 0.0059 0.001 0.001 0.0001 0.0008 0.002 0.0012 0.001
Bud Periphery 0.0014 0.0002 0.0012 0.0048 0.0002 0.0002 0.0009 0 0.0003 0.0018 0.0065 0.0003 0.0013 0.0001 0.0009 0.0015 0.0011 0.0003
Bud Site 0.0027 0.0003 0.0011 0.0034 0.0003 0.0002 0.0016 0.0001 0.0018 0.0038 0.0555 0.0001 0.0016 0.0001 0.0012 0.0006 0.0011 0.0005
Cell Periphery 0.0004 0 0.0003 0.0006 0 0 0.0002 0 0.0001 0.0012 0.0036 0 0.0002 0 0.0002 0.0002 0.0006 0.0001
Cytoplasm 0.0046 0.0009 0.0015 0.0029 0.0004 0.0003 0.0057 0.0009 0.0017 0.0034 0.012 0.0001 0.0074 0.007 0.0015 0.0004 0.0075 0.0015
Cytoplasmic Foci 0.0145 0.0007 0.0035 0.0081 0.0044 0.001 0.0282 0 0.0058 0.0507 0.0443 0.0012 0.0068 0.0001 0.0016 0.0065 0.0116 0.0005
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0002 0 0.0002 0.0003 0.0004 0 0.0001 0 0.0004 0.0001 0.0008 0.0001
Endoplasmic Reticulum 0.005 0.0001 0.0038 0.0013 0.0004 0.0001 0.0025 0.0001 0.0003 0.0029 0.0099 0.0003 0.0029 0.0003 0.001 0.0007 0.0059 0.0003
Endosome 0.0289 0.002 0.014 0.0299 0.0139 0.0076 0.0265 0.0001 0.003 0.039 0.056 0.0016 0.0145 0.0002 0.0028 0.0031 0.0234 0.0012
Golgi 0.0075 0.0006 0.0075 0.0166 0.006 0.0046 0.0062 0 0.005 0.0129 0.0151 0.0011 0.0034 0 0.0013 0.0016 0.0112 0.0008
Lipid Particles 0.0239 0.0042 0.002 0.0115 0.0097 0.001 0.0187 0 0.0078 0.0742 0.0157 0.0092 0.009 0 0.0021 0.007 0.0389 0.0043
Mitochondria 0.0193 0.0011 0.0094 0.0453 0.0401 0.0058 0.0126 0.0012 0.0081 0.0063 0.055 0.001 0.0055 0.0003 0.0077 0.0041 0.0159 0.0075
None 0.0052 0.0061 0.0003 0.0006 0.0004 0.0002 0.0053 0.0001 0.011 0.0137 0.0052 0.0001 0.0062 0.0028 0.005 0.0003 0.0101 0.0011
Nuclear Periphery 0.0386 0.0193 0.0132 0.0124 0.0032 0.0023 0.0108 0.0064 0.0044 0.0043 0.0268 0.0056 0.022 0.0076 0.0058 0.0067 0.0194 0.0031
Nucleolus 0.0328 0.0087 0.0134 0.0218 0.0175 0.0293 0.0095 0.0087 0.0052 0.0116 0.0094 0.0148 0.0094 0.0064 0.0076 0.0075 0.0217 0.0144
Nucleus 0.7536 0.9343 0.8947 0.7969 0.8887 0.9271 0.8188 0.9809 0.9203 0.7014 0.5368 0.9557 0.8609 0.9721 0.9288 0.9316 0.7162 0.9469
Peroxisomes 0.0093 0.0004 0.0004 0.0126 0.0068 0.0005 0.0152 0 0.0048 0.0137 0.0229 0.0016 0.0039 0 0.0053 0.0185 0.0058 0.0011
Punctate Nuclear 0.0204 0.0196 0.0229 0.0024 0.004 0.017 0.0174 0.0009 0.0061 0.0041 0.0881 0.0047 0.0233 0.0022 0.0108 0.0018 0.052 0.0139
Vacuole 0.0122 0.0003 0.0055 0.0136 0.0005 0.0009 0.0038 0.0002 0.0003 0.0174 0.0076 0.0002 0.0034 0.0005 0.0004 0.0015 0.0079 0.0003
Vacuole Periphery 0.0059 0.0002 0.003 0.005 0.0008 0.0005 0.0018 0.0002 0.0002 0.0016 0.0042 0.0005 0.0024 0.0002 0.0004 0.0008 0.0058 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.1569 7.2323 6.6052 7.4362 9.2792 5.5979 8.8595 7.7829 6.2426 8.801
Translational Efficiency 1.4499 1.4759 1.5686 1.3967 1.2039 1.8893 1.051 1.2149 1.5469 1.3726

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1608 1055 200 1379 1460 963 1518 418 3068 2018 1718 1797

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 616.92 712.75 1033.55 871.34 744.75 755.64 910.46 912.68 677.75 733.22 924.79 880.96
Standard Deviation 83.98 106.22 114.07 121.08 85.44 94.98 106.27 118.69 106.05 103.26 114.24 121.79
Intensity Change Log 2 — 0.208313 0.744453 0.498152 — 0.020943 0.289839 0.293353 — 0.108858 0.513659 0.389755

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000033 0.000546 0.002238 0.000887 0.000065 0.000425 0.000470 0.001409 0.000049 0.000488 0.000676 0.001009
Bud Neck 0.001996 0.007236 0.005027 0.021034 0.001284 0.004146 0.005109 0.012053 0.001657 0.005761 0.005099 0.018945
Bud Site 0.000602 0.002016 0.002053 0.009271 0.000477 0.002049 0.001121 0.012483 0.000542 0.002032 0.001230 0.010018
Cell Periphery 0.000195 0.000161 0.000166 0.000151 0.000065 0.000259 0.000070 0.000139 0.000133 0.000208 0.000081 0.000148
Cytoplasm 0.105241* 0.067546 0.018737 0.024247 0.062306 0.038213 0.019251 0.024701 0.084809 0.053548 0.019191 0.024353
Cytoplasmic Foci 0.008728 0.014843 0.001881 0.004593 0.009247 0.008976 0.000457 0.003843 0.008975 0.012043 0.000622 0.004419
Eisosomes 0.000021 0.000043 0.000088 0.000027 0.000017 0.000039 0.000023 0.000030 0.000019 0.000041 0.000030 0.000028
Endoplasmic Reticulum 0.000668 0.005481 0.023231 0.008157 0.001475 0.003537 0.005460 0.009080 0.001052 0.004553 0.007529 0.008372
Endosome 0.000358 0.002289 0.001464 0.002935 0.000551 0.005730 0.000439 0.004040 0.000450 0.003931 0.000558 0.003192
Golgi 0.000104 0.001664 0.000184 0.002592 0.000133 0.002252 0.000062 0.006956 0.000118 0.001945 0.000077 0.003607
Lipid Particles 0.001593 0.005005 0.001777 0.001003 0.001729 0.003426 0.000326 0.000798 0.001657 0.004251 0.000495 0.000955
Mitochondria 0.001013 0.007429 0.001196 0.003812 0.000569 0.008426 0.000961 0.005524 0.000802 0.007905 0.000989 0.004210
Mitotic Spindle 0.000339 0.006623 0.000423 0.029180 0.000635 0.005845 0.001806 0.030990 0.000480 0.006252 0.001645 0.029601
None 0.032865 0.017966 0.001648 0.007537 0.006751 0.006587 0.004439 0.007816 0.020438 0.012536 0.004114 0.007602
Nuclear Periphery 0.000840 0.003178 0.002319 0.002641 0.002340 0.002844 0.001352 0.002110 0.001553 0.003019 0.001465 0.002518
Nuclear Periphery Foci 0.000249 0.002346 0.002500 0.000971 0.000792 0.000918 0.000365 0.001263 0.000507 0.001664 0.000613 0.001039
Nucleolus 0.005151 0.013761 0.007536 0.002724 0.003683 0.010127 0.004131 0.002819 0.004452 0.012027 0.004527 0.002746
Nucleus 0.838511* 0.831556* 0.907506* 0.864823* 0.905403* 0.886464* 0.949831* 0.862179* 0.870344* 0.857759* 0.944904* 0.864208*
Peroxisomes 0.000432 0.004210 0.000653 0.000308 0.000857 0.002454 0.000195 0.000389 0.000634 0.003372 0.000248 0.000327
Vacuole 0.000866 0.005027 0.019080 0.011232 0.001464 0.006291 0.004019 0.010611 0.001151 0.005630 0.005772 0.011088
Vacuole Periphery 0.000195 0.001072 0.000294 0.001873 0.000159 0.000992 0.000114 0.000768 0.000178 0.001034 0.000135 0.001616

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.40 -12.48 -13.21 -4.26 6.96 -1.84 -18.00 -6.63 -4.16 -4.32 -4.60 -20.65 -14.12 -5.63 -4.08
Bud Neck -5.74 -7.04 -13.22 -9.16 -10.11 -3.78 -13.64 -10.71 -8.49 -7.24 -6.85 -12.60 -16.31 -12.25 -11.76
Bud Site -3.97 -5.91 -5.80 -4.19 -3.34 -2.93 -3.77 -3.51 -2.81 -2.98 -4.79 -4.65 -6.84 -5.06 -5.37
Cell Periphery 0.80 -0.49 1.56 1.42 3.20 -1.23 -1.69 -4.25 0.79 -3.56 -0.98 2.11 -0.19 0.95 -4.47
Cytoplasm 6.16 11.99 17.67 10.23 -0.38 5.22 11.70 10.42 4.74 0.14 7.98 20.20 20.24 10.78 -0.86
Cytoplasmic Foci -3.69 6.84 6.30 7.87 -0.55 -0.27 7.35 5.29 5.29 -1.58 -2.91 11.75 7.75 9.17 -2.88
Eisosomes -7.38 -9.69 -7.43 2.65 7.83 -2.15 -6.75 -9.27 -1.03 -5.58 -4.58 -9.72 -12.28 0.46 -0.86
Endoplasmic Reticulum -7.83 -14.37 -24.63 -6.57 8.41 -4.36 -18.47 -11.48 -9.04 -6.07 -8.94 -23.06 -25.68 -12.15 -4.01
Endosome -5.41 -6.08 -4.71 1.07 0.33 -4.00 -0.99 -2.89 2.14 -2.76 -5.42 -3.64 -5.50 2.73 -4.45
Golgi -2.22 -3.49 -2.29 0.56 -2.02 -3.37 1.12 -1.46 -0.18 -1.49 -3.86 0.76 -2.62 0.60 -2.66
Lipid Particles -4.23 -1.01 1.83 5.91 4.59 -3.44 5.65 2.27 4.82 -2.48 -5.40 4.31 2.96 7.71 -1.72
Mitochondria -5.59 -1.07 -3.70 3.47 -3.75 -4.79 -9.22 -3.38 2.15 -2.77 -7.19 -2.26 -5.46 4.13 -4.90
Mitotic Spindle -3.55 -1.36 -7.62 -4.97 -7.50 -3.18 -3.97 -4.11 -2.87 -3.56 -4.76 -4.47 -8.64 -5.82 -7.46
None 4.54 12.03 10.21 5.41 -5.13 0.17 2.21 0.68 0.56 -1.62 4.29 10.76 9.44 5.07 -2.34
Nuclear Periphery -6.45 -7.21 -14.07 -1.89 -4.20 -0.93 0.46 -3.53 -2.08 -4.75 -4.55 -3.51 -10.22 -2.90 -8.40
Nuclear Periphery Foci -4.86 -4.62 -4.44 3.01 3.15 -0.58 0.47 -1.57 -1.34 -1.91 -4.27 -1.98 -3.00 1.60 -1.61
Nucleolus -7.00 -1.64 3.34 8.66 3.43 -5.98 -1.07 0.29 5.88 1.16 -9.19 -0.11 2.64 10.35 1.88
Nucleus 1.30 -3.83 -3.30 -4.15 1.46 2.56 -5.78 4.30 2.19 7.30 2.58 -12.46 1.08 -1.38 11.45
Peroxisomes -5.23 -1.69 2.09 5.59 5.87 -3.42 2.05 1.35 5.19 -1.71 -6.25 2.43 2.28 7.44 -0.51
Vacuole -5.98 -7.31 -10.13 -5.95 3.14 -3.49 -7.87 -6.96 -5.33 -4.90 -6.38 -10.72 -12.16 -7.81 -4.74
Vacuole Periphery -2.69 -2.07 -2.33 0.31 -1.86 -4.03 0.55 -2.13 2.43 -2.64 -4.37 0.50 -2.63 0.73 -2.78
Standard name
Human Ortholog
Description Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
Localization
Cell Percentages nucleus (92%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cef1

Cef1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cef1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available