Standard name | |
---|---|
Human Ortholog | |
Description | One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 2 | 4 | 4 | 3 | 2 | 3 | 2 | 2 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 5 | 6 | 10 | 35 | 16 | 26 | 8 | 9 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 3 | 5 | 1 | 2 | 1 | 2 | 3 | 2 | 3 | 5 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 48 | 27 | 130 | 157 | 194 | 209 | 372 | 338 | 236 | 252 | 136 | 189 | 165 | 124 | 264 | 108 | 64 | 61 | 3 | 2 | 6 | 8 | 10 | 21 |
Endoplasmic Reticulum | 1 | 3 | 2 | 3 | 10 | 7 | 4 | 1 | 1 | 6 | 1 | 3 | 24 | 5 | 5 | 9 | 6 | 3 | 10 | 5 | 32 | 8 | 14 | 21 |
Endosome | 4 | 1 | 32 | 6 | 2 | 9 | 13 | 16 | 7 | 5 | 1 | 5 | 4 | 0 | 1 | 4 | 6 | 8 | 1 | 1 | 9 | 3 | 7 | 12 |
Golgi | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 2 |
Mitochondria | 11 | 3 | 1 | 1 | 0 | 0 | 6 | 3 | 4 | 16 | 7 | 8 | 0 | 0 | 0 | 6 | 7 | 1 | 2 | 1 | 9 | 4 | 4 | 4 |
Nucleus | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 0 | 2 | 0 | 1 | 0 | 54 | 23 | 21 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 2 | 0 | 1 | 0 | 4 | 2 | 1 | 0 | 1 | 2 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 50 | 5 | 56 | 15 | 18 | 9 | 51 | 48 | 32 | 44 | 9 | 23 | 14 | 4 | 9 | 197 | 130 | 132 | 106 | 71 | 138 | 87 | 153 | 144 |
Unique Cell Count | 106 | 36 | 189 | 174 | 220 | 232 | 426 | 388 | 273 | 299 | 150 | 216 | 198 | 132 | 282 | 308 | 188 | 192 | 130 | 87 | 206 | 116 | 198 | 213 |
Labelled Cell Count | 119 | 42 | 229 | 193 | 241 | 250 | 494 | 431 | 314 | 341 | 171 | 246 | 214 | 137 | 292 | 383 | 237 | 228 | 130 | 87 | 206 | 116 | 198 | 213 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.8 | 6.6 | 6.8 | 7.4 | 6.9 | 6.4 | 6.2 | 6.1 | 5.6 | 5.3 | 5.0 | 7.5 | 6.7 | 6.5 | 9.6 | 9.1 | 9.3 | 8.0 | 8.4 | 8.9 |
Std Deviation (1e-4) | 0.8 | 0.9 | 1.8 | 1.6 | 1.5 | 1.6 | 1.5 | 1.5 | 1.7 | 1.4 | 1.7 | 1.2 | 1.7 | 1.1 | 1.8 | 2.8 | 2.4 | 2.5 | 1.3 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.17 | 0.06 | -0.04 | -0.07 | -0.11 | -0.24 | -0.31 | -0.39 | 0.2 | 0.02 | -0.01 | 0.55 | 0.46 | 0.5 | 0.28 | 0.35 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 4.1 | 2.8 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5.0 | 4.8 | 5.5 | 5.5 | 5.3 | 4.6 | 4.0 | 4.9 | 4.6 | 3.4 | 5.5 | 7.1 | -7.3 | -6.7 | -7.2 |
Endoplasmic Reticulum | 0 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 0 | 0 | 0 | 0 | 0 |
Endosome | -4.2 | -5.9 | -4.5 | -6.1 | -5.2 | -5.5 | -6.2 | -5.0 | -5.1 | -5.0 | -5.0 | -6.9 | -6.5 | -4.4 | -4.1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.1 | 5.0 | 4.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.0 | -5.6 | -7.3 | -5.3 | -5.1 | -4.8 | -4.0 | -5.5 | -4.8 | -5.8 | -6.0 | -8.2 | 7.4 | 7.7 | 7.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4114 | 5.0921 | 4.539 | 4.1792 | 4.3262 | 4.5443 | 2.9333 | 4.6856 | 4.3909 | 3.4347 | 3.2724 | 4.2949 | 2.9959 | 4.7753 | 4.0046 | 4.1767 | 3.5623 | 3.9871 |
Actin | 0.0169 | 0 | 0.0004 | 0 | 0.0002 | 0.0008 | 0.0193 | 0.0001 | 0.0115 | 0.013 | 0.0631 | 0.0014 | 0.005 | 0 | 0.0023 | 0.0001 | 0.0403 | 0 |
Bud | 0.0006 | 0.0022 | 0.0005 | 0.0005 | 0.0013 | 0.0004 | 0.0008 | 0.0004 | 0.001 | 0.0009 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0004 | 0.0041 | 0.0004 | 0.0004 |
Bud Neck | 0.0015 | 0.0017 | 0.0015 | 0.0009 | 0.002 | 0.002 | 0.0021 | 0.0022 | 0.0038 | 0.0013 | 0.0014 | 0.0037 | 0.0013 | 0.0015 | 0.0013 | 0.0023 | 0.0014 | 0.0021 |
Bud Periphery | 0.0012 | 0.0014 | 0.0015 | 0.0012 | 0.0023 | 0.002 | 0.0011 | 0.0009 | 0.0018 | 0.0016 | 0.0011 | 0.0022 | 0.0015 | 0.0013 | 0.0011 | 0.0087 | 0.001 | 0.0019 |
Bud Site | 0.002 | 0.0016 | 0.0025 | 0.0008 | 0.005 | 0.0011 | 0.0071 | 0.0032 | 0.0125 | 0.0022 | 0.0017 | 0.0014 | 0.0015 | 0.0017 | 0.0013 | 0.0118 | 0.0013 | 0.0007 |
Cell Periphery | 0.0075 | 0.0113 | 0.0104 | 0.0041 | 0.0045 | 0.0072 | 0.0039 | 0.006 | 0.0065 | 0.0024 | 0.004 | 0.0054 | 0.0113 | 0.0152 | 0.0072 | 0.0116 | 0.0112 | 0.0142 |
Cytoplasm | 0.0239 | 0.0109 | 0.0353 | 0.0355 | 0.0123 | 0.0081 | 0.0299 | 0.0493 | 0.0469 | 0.0048 | 0.0021 | 0.0142 | 0.0304 | 0.0246 | 0.0213 | 0.0099 | 0.0046 | 0.0076 |
Cytoplasmic Foci | 0.0114 | 0.0099 | 0.0192 | 0.0091 | 0.0117 | 0.0079 | 0.0288 | 0.0071 | 0.0128 | 0.0347 | 0.0115 | 0.0072 | 0.0162 | 0.0074 | 0.0066 | 0.0054 | 0.0115 | 0.0033 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.035 | 0.0319 | 0.0489 | 0.0125 | 0.0092 | 0.0253 | 0.0428 | 0.0291 | 0.0342 | 0.0031 | 0.008 | 0.0125 | 0.0481 | 0.0689 | 0.0608 | 0.0173 | 0.0049 | 0.0276 |
Endosome | 0.2095 | 0.0762 | 0.1436 | 0.1947 | 0.2145 | 0.1428 | 0.2534 | 0.1195 | 0.2076 | 0.2092 | 0.209 | 0.1065 | 0.206 | 0.07 | 0.1512 | 0.1299 | 0.0871 | 0.0942 |
Golgi | 0.0051 | 0.0011 | 0.004 | 0.0034 | 0.0058 | 0.0034 | 0.0222 | 0.0021 | 0.0138 | 0.0598 | 0.0877 | 0.0038 | 0.0069 | 0.0018 | 0.0061 | 0.0029 | 0.0108 | 0.0026 |
Lipid Particles | 0.0101 | 0.0044 | 0.0072 | 0.0096 | 0.0046 | 0.0133 | 0.0224 | 0.0097 | 0.013 | 0.0181 | 0.0174 | 0.0107 | 0.0139 | 0.0083 | 0.0147 | 0.0018 | 0.0209 | 0.0107 |
Mitochondria | 0.0023 | 0.0015 | 0.0015 | 0.001 | 0.0012 | 0.0024 | 0.0071 | 0.0007 | 0.0059 | 0.0249 | 0.0246 | 0.0008 | 0.0046 | 0.0011 | 0.0015 | 0.01 | 0.0272 | 0.0013 |
None | 0.0156 | 0.0008 | 0.0031 | 0.0004 | 0.0003 | 0.0002 | 0.0046 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0023 | 0.001 | 0.0004 | 0.0003 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.0261 | 0.0077 | 0.0123 | 0.0046 | 0.0186 | 0.0133 | 0.0516 | 0.0122 | 0.0071 | 0.0036 | 0.0029 | 0.0148 | 0.0124 | 0.0133 | 0.0117 | 0.0055 | 0.0031 | 0.0072 |
Nucleolus | 0.003 | 0.0025 | 0.0016 | 0.0007 | 0.0021 | 0.0009 | 0.0023 | 0.0035 | 0.0024 | 0.0021 | 0.0028 | 0.0033 | 0.0022 | 0.0018 | 0.0019 | 0.0021 | 0.0007 | 0.0012 |
Nucleus | 0.0169 | 0.0149 | 0.0166 | 0.0022 | 0.0176 | 0.0037 | 0.0105 | 0.0079 | 0.0066 | 0.0027 | 0.0017 | 0.0083 | 0.0048 | 0.0038 | 0.0044 | 0.0051 | 0.0019 | 0.0038 |
Peroxisomes | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0064 | 0.0001 | 0.0015 | 0.0112 | 0.0009 | 0.0074 | 0.0005 | 0.0001 | 0.0022 | 0.0001 | 0.0287 | 0.0002 |
Punctate Nuclear | 0.0022 | 0.0028 | 0.0042 | 0.0001 | 0.001 | 0.0003 | 0.0045 | 0.0003 | 0.0011 | 0.001 | 0.0003 | 0.0037 | 0.0016 | 0.0002 | 0.0004 | 0.0001 | 0.0015 | 0.0001 |
Vacuole | 0.5643 | 0.7858 | 0.643 | 0.6543 | 0.6428 | 0.7068 | 0.4303 | 0.6975 | 0.5633 | 0.5462 | 0.4883 | 0.7387 | 0.587 | 0.7375 | 0.6551 | 0.6862 | 0.6993 | 0.7787 |
Vacuole Periphery | 0.0442 | 0.031 | 0.042 | 0.0641 | 0.0428 | 0.0577 | 0.0488 | 0.0477 | 0.0461 | 0.0568 | 0.0708 | 0.0531 | 0.0419 | 0.0401 | 0.048 | 0.0846 | 0.0414 | 0.0422 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.8129 | 38.2721 | 38.0812 | 36.453 | 52.3549 | 32.0919 | 41.5552 | 35.9119 | 50.66 | 46.1954 |
Translational Efficiency | 1.8848 | 1.5167 | 1.2439 | 1.565 | 1.3136 | 1.9273 | 1.3299 | 1.4282 | 1.0648 | 1.3897 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
183 | 186 | 384 | 1175 | 2282 | 1219 | 524 | 1105 | 2465 | 1405 | 908 | 2280 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 902.79 | 950.80 | 1066.87 | 1051.84 | 807.81 | 859.23 | 1068.11 | 1125.81 | 814.86 | 871.35 | 1067.59 | 1087.69 |
Standard Deviation | 115.64 | 132.76 | 118.75 | 151.15 | 104.01 | 104.81 | 126.49 | 164.06 | 107.83 | 113.25 | 123.28 | 161.82 |
Intensity Change Log 2 | — | 0.074751 | 0.240922 | 0.220453 | — | 0.089028 | 0.402972 | 0.478875 | — | 0.081511 | 0.319720 | 0.348270 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000189 | 0.000130 | 0.005135 | 0.025551 | 0.000152 | 0.000181 | 0.002424 | 0.019761 | 0.000155 | 0.000174 | 0.003571 | 0.022745 |
Bud Neck | 0.017868 | 0.011491 | 0.007663 | 0.020716 | 0.024163 | 0.012448 | 0.012378 | 0.023484 | 0.023695 | 0.012322 | 0.010384 | 0.022058 |
Bud Site | 0.012214 | 0.009335 | 0.011569 | 0.063243 | 0.010373 | 0.012756 | 0.013019 | 0.053287 | 0.010510 | 0.012303 | 0.012406 | 0.058418 |
Cell Periphery | 0.001875 | 0.001353 | 0.003040 | 0.007558 | 0.000364 | 0.001116 | 0.001782 | 0.014536 | 0.000476 | 0.001148 | 0.002314 | 0.010940 |
Cytoplasm | 0.037279 | 0.056748 | 0.193154* | 0.203787* | 0.186739* | 0.043043 | 0.266082* | 0.208019* | 0.175644* | 0.044857 | 0.235240* | 0.205838* |
Cytoplasmic Foci | 0.069271 | 0.058158 | 0.090857 | 0.064908 | 0.232253* | 0.024806 | 0.095963 | 0.042242 | 0.220154* | 0.029221 | 0.093803 | 0.053923 |
Eisosomes | 0.000239 | 0.000042 | 0.000956 | 0.002625 | 0.000194 | 0.000103 | 0.000824 | 0.004129 | 0.000197 | 0.000095 | 0.000880 | 0.003354 |
Endoplasmic Reticulum | 0.019719 | 0.010631 | 0.164901* | 0.151936* | 0.010692 | 0.017382 | 0.069231 | 0.223026* | 0.011362 | 0.016488 | 0.109690* | 0.186390* |
Endosome | 0.128335 | 0.084342 | 0.029713 | 0.033689 | 0.134536 | 0.058992 | 0.022416 | 0.023144 | 0.134076 | 0.062347 | 0.025502 | 0.028578 |
Golgi | 0.003955 | 0.001515 | 0.001878 | 0.009057 | 0.003501 | 0.004588 | 0.000878 | 0.012168 | 0.003534 | 0.004181 | 0.001301 | 0.010565 |
Lipid Particles | 0.015778 | 0.005847 | 0.050588 | 0.077526 | 0.040703 | 0.006472 | 0.032329 | 0.109605* | 0.038852 | 0.006389 | 0.040051 | 0.093073 |
Mitochondria | 0.000518 | 0.000179 | 0.000152 | 0.001848 | 0.001336 | 0.003848 | 0.000182 | 0.000885 | 0.001275 | 0.003362 | 0.000169 | 0.001382 |
Mitotic Spindle | 0.001124 | 0.002505 | 0.002238 | 0.005985 | 0.006247 | 0.001775 | 0.000614 | 0.002703 | 0.005867 | 0.001871 | 0.001301 | 0.004394 |
None | 0.000050 | 0.000027 | 0.000444 | 0.000132 | 0.001421 | 0.000225 | 0.000501 | 0.000289 | 0.001319 | 0.000199 | 0.000477 | 0.000208 |
Nuclear Periphery | 0.000424 | 0.000333 | 0.004095 | 0.000558 | 0.000613 | 0.000722 | 0.002427 | 0.000268 | 0.000599 | 0.000670 | 0.003132 | 0.000418 |
Nuclear Periphery Foci | 0.004827 | 0.004169 | 0.057884 | 0.013553 | 0.009014 | 0.000756 | 0.033628 | 0.015168 | 0.008703 | 0.001208 | 0.043886 | 0.014336 |
Nucleolus | 0.000727 | 0.000237 | 0.000302 | 0.000207 | 0.003211 | 0.000894 | 0.000423 | 0.000171 | 0.003026 | 0.000807 | 0.000372 | 0.000189 |
Nucleus | 0.003992 | 0.005580 | 0.059034 | 0.015326 | 0.016857 | 0.010121 | 0.079031 | 0.016068 | 0.015902 | 0.009520 | 0.070574 | 0.015686 |
Peroxisomes | 0.001964 | 0.003206 | 0.001650 | 0.004740 | 0.004660 | 0.001915 | 0.001793 | 0.003813 | 0.004460 | 0.002086 | 0.001732 | 0.004291 |
Vacuole | 0.666639* | 0.737738* | 0.314396* | 0.294253* | 0.308568* | 0.785357* | 0.363753* | 0.225250* | 0.335151* | 0.779053* | 0.342879* | 0.260811* |
Vacuole Periphery | 0.013015 | 0.006435 | 0.000352 | 0.002805 | 0.004403 | 0.012500 | 0.000324 | 0.001981 | 0.005042 | 0.011697 | 0.000336 | 0.002406 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.80 | -2.54 | -10.86 | -10.89 | -6.69 | -0.77 | -4.21 | -11.16 | -11.14 | -9.51 | -0.60 | -3.92 | -15.42 | -15.40 | -11.31 |
Bud Neck | 1.83 | 3.26 | -0.64 | -3.59 | -7.36 | 7.61 | 6.11 | 0.49 | -5.85 | -5.04 | 7.95 | 8.81 | 1.44 | -6.95 | -7.92 |
Bud Site | 0.82 | 0.13 | -10.36 | -13.75 | -13.36 | -1.39 | -1.80 | -12.24 | -10.60 | -10.49 | -1.17 | -1.83 | -18.83 | -16.00 | -16.75 |
Cell Periphery | 0.98 | -1.22 | -7.03 | -7.19 | -4.34 | -6.33 | -3.75 | -11.14 | -10.49 | -9.70 | -5.47 | -4.67 | -14.44 | -13.35 | -10.65 |
Cytoplasm | -2.01 | -13.06 | -19.30 | -14.29 | -1.29 | 25.02 | -6.48 | -2.96 | -23.27 | 4.12 | 24.14 | -6.33 | -4.99 | -30.41 | 2.69 |
Cytoplasmic Foci | 0.85 | -1.82 | 0.50 | -0.68 | 4.49 | 38.44* | 21.06 | 34.53 | -6.27 | 10.48 | 36.67 | 22.28 | 32.51 | -9.48 | 10.28 |
Eisosomes | 1.76 | -3.45 | -8.33 | -10.06 | -5.41 | 1.37 | -4.31 | -8.94 | -9.22 | -7.33 | 1.68 | -5.73 | -12.23 | -12.92 | -9.17 |
Endoplasmic Reticulum | 1.73 | -10.94 | -15.36 | -15.61 | 0.95 | -4.46 | -9.19 | -23.27 | -22.31 | -14.24 | -3.57 | -14.61 | -28.87 | -27.53 | -8.82 |
Endosome | 2.75 | 7.84 | 7.63 | 5.07 | -1.15 | 16.79 | 31.17* | 31.00* | 11.60 | -0.47 | 16.37 | 31.66 | 30.96 | 11.46 | -1.71 |
Golgi | 2.29 | 2.24 | -3.39 | -7.84 | -8.38 | -1.30 | 13.20 | -5.06 | -3.84 | -6.82 | -0.89 | 10.86 | -7.19 | -4.94 | -10.06 |
Lipid Particles | 2.50 | -5.95 | -11.13 | -16.53 | -4.48 | 15.40 | 2.82 | -12.17 | -19.76 | -13.24 | 15.64 | 0.06 | -13.88 | -25.95 | -12.99 |
Mitochondria | 1.70 | 1.86 | -2.12 | -2.92 | -2.97 | -2.63 | 11.09 | 1.94 | 3.06 | -2.87 | -2.51 | 11.50 | -0.00 | 2.39 | -3.85 |
Mitotic Spindle | -0.78 | -1.61 | -3.35 | -1.33 | -0.48 | 4.49 | 6.52 | 2.23 | -1.09 | -2.03 | 4.30 | 3.35 | 1.51 | -2.45 | -1.77 |
None | 0.96 | -1.80 | -2.77 | -4.42 | 1.42 | 5.85 | 4.49 | 5.71 | -0.29 | 1.37 | 5.99 | 4.34 | 6.46 | 0.15 | 2.14 |
Nuclear Periphery | 0.51 | -6.75 | -1.15 | -1.36 | 6.44 | -0.71 | -5.68 | 7.73 | 3.27 | 6.86 | -0.51 | -8.56 | 2.72 | 1.86 | 9.11 |
Nuclear Periphery Foci | 0.17 | -9.86 | -4.33 | -4.41 | 8.58 | 8.72 | -8.51 | -4.31 | -15.30 | 6.62 | 8.23 | -12.26 | -4.76 | -16.49 | 10.68 |
Nucleolus | 2.15 | 1.53 | 2.32 | 0.75 | 1.75 | 5.48 | 8.95 | 9.71 | 3.03 | 4.23 | 5.82 | 9.07 | 9.76 | 2.99 | 4.05 |
Nucleus | -0.71 | -11.77 | -10.85 | -4.22 | 9.06 | 4.19 | -13.48 | -0.63 | -3.88 | 12.92 | 4.38 | -16.41 | -1.50 | -5.10 | 15.79 |
Peroxisomes | -0.79 | 0.44 | -4.76 | -0.76 | -5.65 | 5.61 | 9.61 | 3.28 | -3.11 | -5.06 | 5.05 | 10.64 | 1.78 | -3.75 | -7.55 |
Vacuole | -2.15 | 12.41 | 13.55 | 17.92 | 0.61 | -47.59* | -6.25 | 5.99 | 50.62* | 10.39 | -45.56* | -3.00 | 5.32 | 53.87* | 7.74 |
Vacuole Periphery | 2.90 | 5.89 | 4.65 | 3.77 | -3.25 | -9.83 | 19.03 | 5.22 | 11.68 | -3.75 | -8.94 | 18.11 | 5.72 | 11.58 | -4.70 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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