Standard name
Human Ortholog
Description SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0.11 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.97 0.99 0.94 0.91 0.85 0.84 0.81 0.74 0.83 0.97 0.99 0.97 0.95 0.97 0.97 0.95 0.93 0.96 0.92 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0.07 0.07 0 0 0 0 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.08 0.09 0.17 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0.06 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 2 2 1 3 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 2 1 2 1 3 1 5 3 7 2 5 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 13 7 9 12 6 10 12 18 19 25 6 9 8 14 7 7 0 0 0 0 0 0
Cytoplasm 179 155 205 249 328 359 377 398 283 339 264 300 310 341 384 117 98 98 169 151 212 152 327 303
Endoplasmic Reticulum 2 1 2 3 3 6 18 12 11 16 24 20 3 0 6 9 7 1 1 1 0 8 11 9
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 1 1 0 1 1
Mitochondria 1 1 0 0 0 9 19 7 27 37 60 27 0 1 2 0 2 1 0 1 1 0 1 1
Nucleus 1 0 2 2 1 2 3 9 2 5 2 0 0 3 1 8 2 1 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 0 2 0 9 1 46 20 37 18 15 1 1 2 0 1 1 2 1 4 2 11 9
Unique Cell Count 184 156 209 258 331 380 415 469 336 417 359 360 318 346 395 123 101 101 179 163 221 167 362 334
Labelled Cell Count 187 160 224 264 343 399 428 487 362 457 399 390 326 356 403 148 117 111 179 163 221 167 362 334


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 8.5 9.5 9.2 9.6 8.1 7.9 8.3 7.6 7.6 7.1 7.8 10.3 10.4 10.2 12.8 12.6 11.9 9.4 9.4 10.2
Std Deviation (1e-4) 1.5 1.5 1.0 1.0 1.1 1.1 1.1 1.1 1.1 1.2 1.2 1.5 1.4 1.4 1.3 2.4 2.3 2.3 1.0 1.3 1.4
Intensity Change (Log2) – – – -0.05 0.02 -0.22 -0.27 -0.19 -0.32 -0.31 -0.42 -0.28 0.13 0.13 0.11 0.44 0.41 0.33 -0.01 -0.01 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.9 -2.0 -1.8 -3.3 -2.7 -1.4 -1.0 -0.5 0.3 -2.6 -2.1 -2.7 1.7 0.2 0.2
Cytoplasm -1.0 1.0 -2.1 -3.4 -5.1 -5.1 -5.9 -7.4 -5.4 -0.5 0.4 -0.7 -1.5 -0.6 -0.6
Endoplasmic Reticulum 0 0 0 2.3 0 0 2.0 3.2 2.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 0 4.2 4.4 6.2 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.7 3.6 4.4 3.3 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0575 5.3806 4.7886 4.6864 3.97 5.0578 4.8927 6.8395 6.4281 6.032 6.2873 6.2994 5.9821 7.7955 7.1407 6.6028 6.2404 6.7728
Actin 0.0418 0.0002 0.0398 0.0192 0.0298 0.0083 0.0187 0.0003 0.0003 0.0002 0.0002 0.0022 0.0266 0.0012 0.0143 0.0377 0.0155 0.003
Bud 0.0007 0.0001 0.0008 0.0003 0.0051 0.0006 0.0005 0.0001 0.0001 0.0001 0.0001 0.0007 0.0034 0.0003 0.0014 0.0006 0.0009 0.0002
Bud Neck 0.0141 0.0002 0.0012 0.0003 0.0025 0.001 0.0008 0.0001 0.0002 0.0002 0.0003 0.0006 0.0012 0.0001 0.0009 0.001 0.001 0.0007
Bud Periphery 0.0009 0 0.0007 0.0003 0.0114 0.0005 0.0006 0 0 0 0 0.0013 0.0014 0.0001 0.0008 0.0006 0.0011 0.0002
Bud Site 0.0065 0.0008 0.0064 0.0006 0.006 0.0003 0.0023 0.0002 0.0013 0.0001 0.0001 0.0008 0.0009 0.0046 0.0021 0.0216 0.0017 0.0004
Cell Periphery 0.0006 0.0001 0.0003 0 0.0002 0.0001 0.0005 0 0 0 0 0.0001 0.0001 0 0.0001 0.0003 0.0002 0
Cytoplasm 0.7211 0.9774 0.8321 0.8658 0.765 0.8833 0.7053 0.9888 0.9694 0.9109 0.9229 0.9603 0.8667 0.9776 0.9204 0.9066 0.7975 0.9317
Cytoplasmic Foci 0.0389 0.002 0.0162 0.0167 0.0418 0.0134 0.0502 0.0021 0.0057 0.0032 0.0074 0.0038 0.0312 0.0032 0.0167 0.0034 0.0108 0.0158
Eisosomes 0.0005 0 0.0001 0 0.0002 0 0.0002 0 0 0 0 0 0.0002 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0223 0.0117 0.0047 0.0121 0.0109 0.0075 0.016 0.002 0.0013 0.0054 0.0008 0.004 0.0068 0.0014 0.0036 0.0035 0.0008 0.0048
Endosome 0.0452 0.001 0.0424 0.0216 0.0447 0.037 0.0723 0.0015 0.0131 0.0292 0.0352 0.008 0.0132 0.0009 0.0117 0.0077 0.0318 0.0199
Golgi 0.0193 0.0001 0.0109 0.012 0.0283 0.0181 0.0128 0.0001 0.0045 0.0095 0.0047 0.0024 0.0106 0.0004 0.0089 0.0068 0.0544 0.0095
Lipid Particles 0.0125 0 0.0062 0.0032 0.0084 0.0039 0.0323 0.0005 0.0003 0.0216 0.0229 0.0006 0.0041 0.0011 0.0081 0.0028 0.008 0.0007
Mitochondria 0.0174 0.0001 0.0065 0.0035 0.0116 0.003 0.0118 0 0.0002 0.0104 0.0001 0.01 0.0083 0.0001 0.0018 0.001 0.0685 0.0018
None 0.0289 0.0036 0.0052 0.002 0.004 0.0017 0.0091 0.0028 0.0019 0.0013 0.0008 0.0011 0.005 0.0014 0.0017 0.0016 0.0005 0.0008
Nuclear Periphery 0.0099 0.0008 0.0037 0.0242 0.0123 0.0034 0.0119 0.0004 0.0003 0.0013 0.0002 0.0007 0.0024 0.0004 0.0008 0.0013 0.0005 0.0014
Nucleolus 0.0008 0 0.0041 0.0001 0.0009 0.0002 0.0009 0 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0
Nucleus 0.0045 0.0011 0.0064 0.0039 0.0038 0.0124 0.0054 0.0006 0.0006 0.0009 0.0007 0.0009 0.0021 0.0006 0.0008 0.001 0.0008 0.0012
Peroxisomes 0.0047 0.0001 0.0021 0.0097 0.0046 0.0006 0.0196 0 0.0001 0.0008 0.0011 0.0005 0.0136 0.0048 0.0039 0.0007 0.0023 0.0044
Punctate Nuclear 0.0022 0.0002 0.0027 0.001 0.0034 0.0006 0.0087 0.0001 0.0001 0.0002 0.0001 0.0001 0.001 0.0015 0.0011 0.0005 0.0002 0.0003
Vacuole 0.005 0.0005 0.0057 0.0018 0.0031 0.0031 0.0171 0.0004 0.0006 0.0026 0.0017 0.001 0.0007 0.0002 0.0004 0.0009 0.0019 0.0023
Vacuole Periphery 0.0022 0 0.0015 0.0017 0.0017 0.0012 0.0031 0 0.0001 0.0018 0.0005 0.0005 0.0004 0 0.0002 0.0003 0.0014 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.8194 38.0567 32.8369 38.3138 42.36 42.0536 44.1838 45.4489 41.4578 49.3723
Translational Efficiency 0.7779 0.8739 0.7403 0.69 0.6843 0.7486 0.7811 0.6437 0.6935 0.5723

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1481 1345 1324 1574 1572 1434 1461 2315 3053 2779 2785 3889

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 846.53 1111.88 1245.78 1206.10 1084.50 1040.97 1259.18 1263.94 969.06 1075.29 1252.81 1240.53
Standard Deviation 125.65 177.81 174.28 136.29 135.18 153.86 162.08 161.49 176.67 169.63 168.13 154.43
Intensity Change Log 2 — 0.393368 0.557416 0.510716 — -0.059102 0.215454 0.220898 — 0.156877 0.375417 0.355164

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000136 0.000246 0.000501 0.001573 0.000155 0.000264 0.000377 0.003017 0.000146 0.000255 0.000436 0.002433
Bud Neck 0.002130 0.001820 0.001468 0.002655 0.000944 0.001910 0.001556 0.003516 0.001520 0.001866 0.001514 0.003167
Bud Site 0.008154 0.011434 0.007828 0.033183 0.005528 0.011661 0.010423 0.018561 0.006802 0.011551 0.009189 0.024479
Cell Periphery 0.000116 0.000092 0.000162 0.000159 0.000137 0.000121 0.000113 0.000061 0.000127 0.000107 0.000136 0.000101
Cytoplasm 0.744622* 0.794863* 0.811988* 0.675205* 0.783867* 0.700958* 0.739455* 0.830377* 0.764829* 0.746407* 0.773937* 0.767574*
Cytoplasmic Foci 0.112868 0.096443 0.031121 0.042059 0.109193 0.151920 0.036199 0.015888 0.110976 0.125070 0.033785 0.026480
Eisosomes 0.000021 0.000011 0.000008 0.000017 0.000016 0.000024 0.000015 0.000009 0.000018 0.000018 0.000012 0.000012
Endoplasmic Reticulum 0.002665 0.001470 0.000977 0.001349 0.005641 0.002840 0.001334 0.000153 0.004197 0.002177 0.001164 0.000637
Endosome 0.010563 0.005763 0.002351 0.008373 0.004022 0.011853 0.004493 0.001200 0.007195 0.008906 0.003475 0.004103
Golgi 0.002906 0.002736 0.000324 0.008234 0.002884 0.003433 0.000414 0.000492 0.002895 0.003096 0.000371 0.003626
Lipid Particles 0.002258 0.001431 0.002325 0.002582 0.001955 0.002319 0.002116 0.000778 0.002102 0.001889 0.002215 0.001508
Mitochondria 0.001076 0.000475 0.000058 0.001943 0.000189 0.001109 0.000071 0.000667 0.000619 0.000802 0.000065 0.001183
Mitotic Spindle 0.000174 0.004687 0.005341 0.029503 0.000996 0.001858 0.002521 0.013697 0.000597 0.003227 0.003862 0.020094
None 0.004554 0.002064 0.003432 0.001790 0.002628 0.001161 0.002285 0.002690 0.003562 0.001598 0.002830 0.002326
Nuclear Periphery 0.000132 0.000088 0.000273 0.000700 0.000097 0.000093 0.000315 0.000110 0.000114 0.000091 0.000295 0.000349
Nuclear Periphery Foci 0.002271 0.004183 0.010396 0.009406 0.003736 0.003275 0.011362 0.001627 0.003025 0.003715 0.010903 0.004775
Nucleolus 0.000652 0.000230 0.000090 0.000128 0.000233 0.000370 0.000144 0.000147 0.000436 0.000302 0.000119 0.000139
Nucleus 0.025970 0.020410 0.070788 0.043036 0.027040 0.015903 0.049915 0.050418 0.026521 0.018084 0.059838 0.047430
Peroxisomes 0.000765 0.000869 0.000395 0.000879 0.000298 0.001388 0.000328 0.001045 0.000525 0.001137 0.000360 0.000978
Vacuole 0.075686 0.049920 0.050069 0.133542 0.049374 0.085926 0.136252 0.055435 0.062138 0.068500 0.095280 0.087047
Vacuole Periphery 0.002280 0.000767 0.000105 0.003685 0.001069 0.001610 0.000313 0.000113 0.001657 0.001202 0.000214 0.001559

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.65 -7.02 -3.58 -3.02 -1.00 -3.76 -2.78 -2.26 -2.03 -1.71 -5.25 -6.47 -3.18 -2.81 -1.87
Bud Neck 0.90 -0.39 -0.55 -1.61 -0.18 -6.75 -5.55 -3.78 -0.43 -1.15 -1.72 -2.73 -2.87 -1.23 -0.92
Bud Site -2.43 -1.79 -5.96 -3.34 -4.74 -6.44 -3.02 -3.67 1.78 -0.63 -5.89 -3.46 -6.63 -1.31 -3.63
Cell Periphery 3.18 -7.25 -2.46 -5.99 5.75 1.94 3.37 11.26 9.30 9.87 3.52 -4.09 6.95 3.13 10.18
Cytoplasm -5.63 -0.04 16.18 21.94 16.27 10.72 15.07 6.41 -5.65 -10.24 3.55 10.66 14.70 10.14 2.74
Cytoplasmic Foci 3.02 19.49 19.84 16.26 0.18 -8.04 20.79 27.65 31.51 12.19 -3.90 28.45 33.98 34.67 9.30
Eisosomes 6.65 5.85 1.80 -8.45 -7.07 -5.67 -1.28 5.87 10.81 9.16 0.43 3.87 5.63 5.48 2.37
Endoplasmic Reticulum 2.39 4.55 4.98 1.72 1.53 2.92 5.53 6.59 5.17 4.86 3.67 6.81 8.16 5.34 5.05
Endosome 5.30 10.31 8.72 3.68 -1.24 -9.63 1.52 12.26 14.25 5.62 -2.54 9.29 12.76 13.83 3.91
Golgi 0.56 10.01 1.29 0.85 -2.32 -0.69 6.22 6.42 5.81 0.55 -0.27 10.37 5.28 4.73 -2.14
Lipid Particles 2.97 -3.23 -2.99 -6.05 0.36 -1.29 -1.77 5.93 4.19 4.81 1.20 -3.56 0.65 -0.72 4.20
Mitochondria 1.49 2.60 1.23 -0.14 -1.62 -2.02 4.89 -0.83 1.81 -2.29 -0.53 2.93 1.16 1.57 -2.45
Mitotic Spindle -3.70 -6.12 -6.51 -3.59 -2.88 -1.06 -3.20 -4.69 -3.87 -2.48 -3.61 -6.72 -8.09 -4.94 -3.59
None 3.52 2.36 4.28 0.94 4.57 4.10 1.97 0.38 -5.96 -2.82 5.03 3.03 3.78 -3.07 1.41
Nuclear Periphery 1.81 -11.66 -3.97 -4.40 -0.75 0.23 -17.56 -12.93 -11.21 8.28 1.62 -20.10 -6.51 -6.97 1.67
Nuclear Periphery Foci -3.13 -12.61 -12.14 -4.88 4.31 1.22 -9.70 5.93 4.53 12.80 -2.01 -15.73 -5.23 -1.60 12.70
Nucleolus 3.31 4.51 4.20 2.29 -5.08 -1.35 4.81 2.17 1.77 -4.61 1.70 5.22 4.41 2.39 -6.96
Nucleus 3.84 -20.06 -13.96 -17.78 8.80 11.18 -16.47 -23.35 -31.38 -6.09 9.79 -25.55 -26.76 -35.02 2.53
Peroxisomes -0.46 2.21 2.81 2.92 -0.19 -5.67 0.82 -1.38 4.58 -1.67 -4.46 2.37 1.38 5.34 -1.12
Vacuole 6.22 -3.74 -26.16 -30.90 -21.30 -11.53 -25.58 -23.11 -11.75 9.70 -3.40 -21.81 -32.84 -30.14 -6.22
Vacuole Periphery 5.37 7.91 3.56 -0.51 -2.82 -1.24 4.10 4.88 4.33 1.44 1.92 8.66 6.16 3.58 -2.16
Standard name
Human Ortholog
Description SR protein kinase (SRPK); involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sky1

Sky1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sky1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available