Standard name
Human Ortholog
Description Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.46 0.61 0.66 0.71 0.65 0.61 0.58 0.44 0.41 0.38 0.35 0.45 0.59 0.63 0.2 0.22 0.18 0.32 0.31 0.37 0.34 0.29 0.37
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0.06 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.24 0 0.05 0 0.2 0.2 0.2 0.49 0.51 0.59 0.37 0.25 0 0 0.06 0.05 0.06 0.06 0.05 0.05 0.09 0.13 0.07
Nucleus 0.28 0.29 0.33 0.29 0.21 0.18 0.22 0.23 0.25 0.26 0.22 0.35 0.3 0.2 0.2 0.53 0.43 0.55 0.3 0.31 0.27 0.15 0.16 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0.05
Vac/Vac Membrane 0.16 0.15 0 0 0 0 0 0 0 0 0 0 0.08 0.14 0.13 0.23 0.3 0.23 0.15 0.21 0.18 0.3 0.27 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 2
Bud 0 0 2 1 0 2 5 3 10 14 14 3 0 0 0 0 0 0 3 4 5 2 8 9
Bud Neck 0 0 18 9 36 18 30 16 12 7 2 16 0 1 0 0 1 0 0 1 1 0 1 5
Bud Site 0 0 0 0 1 1 6 3 11 7 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 5 1 1 0 2 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Cytoplasm 95 66 188 254 351 339 423 310 251 250 211 172 48 147 135 60 40 32 86 55 140 43 91 126
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 1 0 0 2 1 0 0 8 10 7 1 1 1 2 4 0
Endosome 2 0 0 0 1 0 0 1 0 0 0 1 1 15 5 17 10 11 7 1 1 0 3 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 4 2 2 0 0 0 0 1 3
Mitochondria 62 35 5 19 13 105 140 106 281 309 328 182 26 7 9 18 9 10 17 8 18 11 41 24
Nucleus 65 42 102 112 102 96 156 125 144 157 123 170 32 51 44 155 77 96 83 54 102 19 51 56
Nuclear Periphery 1 0 0 0 0 1 0 0 1 1 0 1 0 0 0 0 0 0 3 0 0 0 0 1
Nucleolus 2 0 0 1 0 1 0 0 0 2 2 1 2 6 2 1 0 0 4 0 0 1 2 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4 0 4 5 12 10 19 20 11 7 3 15 1 7 5 1 1 2 18 5 21 4 11 15
Vac/Vac Membrane 36 21 5 3 17 12 20 16 11 13 17 17 8 36 28 68 54 41 41 38 70 39 86 80
Unique Cell Count 232 144 306 382 493 524 694 533 574 607 556 490 106 251 215 293 181 176 275 178 383 129 315 341
Labelled Cell Count 269 164 331 405 537 585 801 600 734 768 706 584 119 270 228 332 204 201 275 178 383 129 315 341


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.7 4.6 4.1 4.7 3.6 3.8 4.2 3.4 3.4 3.2 3.8 4.1 4.2 4.1 6.4 6.7 6.9 3.9 4.0 4.3
Std Deviation (1e-4) 0.5 0.7 0.6 0.7 0.9 0.9 0.9 0.8 1.1 1.0 0.9 1.0 1.3 1.1 0.8 1.5 1.8 1.8 1.1 0.9 1.2
Intensity Change (Log2) – – – -0.17 0.02 -0.35 -0.27 -0.15 -0.44 -0.45 -0.52 -0.27 -0.16 -0.12 -0.19 0.48 0.54 0.58 -0.24 -0.21 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 8.1 9.2 7.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2373 -0.4076 -0.8385 -0.4886 -0.4848 -0.5713 -0.6411 -0.4307 -0.7756 -0.4685 -0.4967 -0.5578 -0.2684 -0.4089 -0.6448 -0.6589 -0.573 -0.3246
Actin 0.0389 0.0018 0.0055 0.0095 0.0696 0.005 0.0233 0.0002 0.0059 0.0028 0.0079 0.0035 0.1072 0.0003 0.0001 0.0593 0.0014 0.0058
Bud 0.001 0.0008 0.0002 0.0002 0.0005 0.0006 0.0006 0.0001 0.0005 0.0014 0.0014 0.0005 0.0007 0.0001 0.0002 0.0003 0.0003 0.0001
Bud Neck 0.0047 0.0005 0.0023 0.0008 0.0017 0.0058 0.0031 0.0005 0.0007 0.0015 0.0015 0.0023 0.0048 0.0005 0.0006 0.0032 0.0004 0.0025
Bud Periphery 0.0018 0.0007 0.0002 0.0002 0.0006 0.0008 0.001 0 0.0006 0.0011 0.0015 0.0013 0.0009 0.0001 0.0001 0.0002 0.0005 0.0002
Bud Site 0.0097 0.0043 0.0011 0.0004 0.0011 0.0008 0.0131 0.0009 0.0016 0.0047 0.0022 0.0003 0.0074 0.0021 0.0005 0.0007 0.0011 0.0002
Cell Periphery 0.0013 0.0001 0.0001 0 0.0002 0.0002 0.0003 0 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001 0 0.0001 0.0003 0.0001
Cytoplasm 0.0798 0.0695 0.0878 0.0405 0.0471 0.06 0.0812 0.0834 0.0895 0.0756 0.0512 0.0579 0.1143 0.1088 0.1152 0.0978 0.1626 0.1134
Cytoplasmic Foci 0.0376 0.0083 0.0073 0.0112 0.0368 0.0057 0.0398 0.0104 0.004 0.0262 0.0271 0.0115 0.0329 0.0037 0.0128 0.0045 0.0049 0.0044
Eisosomes 0.0008 0.0001 0.0001 0.0001 0.0002 0 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.001 0 0 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0226 0.0052 0.0032 0.0007 0.0021 0.0034 0.0059 0.0024 0.0045 0.0027 0.0026 0.0016 0.012 0.0083 0.0027 0.0012 0.0017 0.0053
Endosome 0.0429 0.0078 0.0168 0.0072 0.0655 0.0121 0.0345 0.0078 0.0062 0.0456 0.0463 0.0058 0.0281 0.004 0.004 0.0017 0.0467 0.0066
Golgi 0.0101 0.0013 0.0048 0.0145 0.03 0.0028 0.0093 0.0002 0.0016 0.027 0.046 0.0033 0.0124 0.0001 0.0002 0.0022 0.0133 0.0025
Lipid Particles 0.0173 0.001 0.0015 0.0051 0.0178 0.0031 0.0294 0.0007 0.0016 0.0157 0.0302 0.0065 0.0176 0.0015 0.0007 0.0009 0.0189 0.0087
Mitochondria 0.0266 0.0014 0.0174 0.0024 0.0097 0.0052 0.0141 0.0004 0.0081 0.0243 0.0692 0.0211 0.0047 0.0005 0.0003 0.0009 0.0334 0.0061
None 0.1339 0.2106 0.2343 0.2619 0.2473 0.0988 0.0678 0.0665 0.1309 0.1365 0.1312 0.0912 0.163 0.088 0.235 0.2009 0.1137 0.111
Nuclear Periphery 0.0479 0.0357 0.0271 0.0077 0.03 0.0104 0.0311 0.0307 0.0369 0.0168 0.0227 0.0165 0.0328 0.0238 0.0167 0.0067 0.0038 0.0218
Nucleolus 0.0055 0.0069 0.0088 0.0047 0.0054 0.0204 0.0053 0.0096 0.0068 0.0041 0.0045 0.0117 0.0057 0.0044 0.0047 0.0023 0.0023 0.0064
Nucleus 0.4211 0.6129 0.5289 0.5787 0.3948 0.7177 0.5857 0.738 0.6506 0.5797 0.4843 0.7323 0.3964 0.7107 0.5316 0.6039 0.5722 0.6609
Peroxisomes 0.0236 0.0015 0.0026 0.0033 0.0116 0.0011 0.0244 0.0001 0.0004 0.0065 0.0142 0.0012 0.0139 0.0001 0.0002 0.0013 0.0012 0.0085
Punctate Nuclear 0.063 0.0248 0.0462 0.0505 0.017 0.0383 0.0215 0.0445 0.0458 0.0218 0.0518 0.0289 0.0361 0.0401 0.072 0.0108 0.0022 0.0338
Vacuole 0.0061 0.0038 0.0026 0.0004 0.0073 0.0062 0.006 0.0031 0.0025 0.0042 0.0025 0.0019 0.0059 0.0021 0.0018 0.0008 0.0099 0.0012
Vacuole Periphery 0.0037 0.001 0.0012 0.0002 0.0036 0.0015 0.0024 0.0005 0.0009 0.0015 0.0013 0.0007 0.0017 0.0006 0.0004 0.0002 0.0091 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.6957 11.8612 10.5618 9.751 6.8813 11.1393 7.4356 10.5227 11.4925 10.4966
Translational Efficiency 0.8834 0.3686 0.5407 0.6955 0.6992 0.511 0.7398 0.561 0.6654 0.5831

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (19%), nucleus (14%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mre11

Mre11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mre11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available