Standard name
Human Ortholog
Description Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.09 0.09 0.07 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.66 0.07 0.67 0.59 0.61 0.28 0.31 0.23 0.1 0.07 0.09 0.17 0.78 0.6 0.64 0.63 0.69 0.65 0.85 0.78 0.84 0.84 0.84 0.86
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.21 0.6 0.31 0.33 0.28 0.6 0.54 0.51 0.67 0.75 0.72 0.61 0 0.06 0 0.1 0.12 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.34 0.39 0.24 0.18 0.19 0.13 0.14 0.12 0.1 0.11 0.08 0.3 0.15 0.18 0.13 0.21 0.24 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.1 0.09 0.12 0.18 0.15 0.14 0.06 0.09 0.18 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0 0.21 0.2 0.19 0.16 0.21 0.28 0.19 0.15 0.17 0.21 0.28 0.31 0.19 0.19 0.18 0.12 0 0 0.05 0.05 0 0
Vac/Vac Membrane 0.29 0.22 0.06 0 0.05 0 0 0 0 0 0 0 0.32 0.34 0.4 0.29 0.27 0.3 0.05 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 1 2 1 0 3 2 0 0 1 0 0 2 0 1 0
Bud 0 2 4 2 1 13 24 49 32 33 33 59 0 1 2 7 6 3 0 0 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 4 1 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 9 14 19 15 18 10 7 13 14 14 1 4 7 39 39 21 0 0 0 0 0 1
Endoplasmic Reticulum 0 1 0 1 0 0 2 2 2 1 2 1 0 2 2 19 27 14 0 0 0 0 1 3
Endosome 285 7 236 147 206 116 167 125 35 29 48 104 62 114 105 321 439 221 400 86 299 168 277 295
Golgi 2 1 2 0 1 0 0 0 0 0 0 0 0 1 2 4 7 17 9 4 6 2 4 5
Mitochondria 92 59 107 82 95 250 286 281 233 333 390 378 0 12 3 52 75 35 6 3 5 2 4 9
Nucleus 7 0 0 0 0 1 3 2 3 0 2 2 0 1 6 8 14 2 1 0 0 0 1 0
Nuclear Periphery 17 7 15 2 8 1 4 3 2 6 4 0 4 11 6 7 10 6 4 0 3 1 4 3
Nucleolus 144 33 135 60 60 79 70 77 40 45 60 51 24 28 29 68 132 81 3 0 1 2 2 1
Peroxisomes 4 0 25 24 30 48 95 84 49 26 50 114 0 0 0 8 3 0 1 0 5 3 5 4
SpindlePole 61 2 75 50 65 65 110 151 66 68 89 133 22 58 31 97 117 42 18 3 19 10 8 6
Vac/Vac Membrane 124 22 21 9 18 13 22 17 3 15 14 23 25 64 65 148 174 100 21 7 4 6 12 12
Unique Cell Count 435 98 350 251 336 417 532 546 347 446 539 623 79 189 164 506 638 338 469 111 356 200 328 345
Labelled Cell Count 737 139 629 391 503 602 803 803 476 571 714 886 138 299 260 778 1043 543 469 111 356 200 328 345


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.2 6.7 6.6 6.6 5.2 5.1 5.0 4.8 4.5 4.6 4.9 7.8 7.2 8.0 6.2 7.3 9.1 8.2 8.3 8.9
Std Deviation (1e-4) 0.9 1.6 1.4 1.5 1.8 1.4 1.1 1.2 0.9 0.9 0.9 1.0 0.8 0.8 1.0 1.1 1.4 1.7 1.3 1.2 1.7
Intensity Change (Log2) – – – -0.03 -0.02 -0.37 -0.4 -0.42 -0.49 -0.58 -0.55 -0.45 0.22 0.1 0.27 -0.1 0.13 0.44 0.29 0.32 0.41

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.9 2.8 4.8 0 0 0 5.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 2.0 0.8 0.7 -0.8 -0.5 0.3 0 -0.3 0 0 1.0 3.2 2.5 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.9 3.8
Endosome -2.2 -1.7 -11.0 -10.5 -13.3 0 0 0 -15.9 1.9 -1.7 -0.8 -1.2 0.4 -0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Mitochondria 0.5 -0.7 8.1 6.8 6.2 0 0 0 9.0 -5.7 -6.5 -7.4 -7.5 -7.3 -6.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.5 -1.4 -3.9 -3.5 -3.9 0 0 0 -5.2 0 0.8 -0.3 -2.6 -2.6 -1.9
Nucleolus -3.8 -6.0 -6.0 -8.7 -8.4 0 0 0 -11.6 -1.4 -5.7 -4.7 -8.5 -6.1 -4.1
Peroxisomes 1.1 0.9 2.1 4.5 3.7 0 0 0 4.8 0 -3.8 -3.5 -4.2 -6.0 -5.0
SpindlePole -0.4 -0.7 -2.1 -0.3 2.1 0 0 0 0 1.2 2.4 -0.7 -0.8 -1.2 -3.1
Vacuole -1.3 -0.4 -1.9 -1.3 -2.1 -3.7 -1.8 -2.5 -1.7 6.7 8.5 9.5 8.4 8.0 8.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.022 0.0003 0.0126 0 0.0039 0.0001 0.0114 0.0001 0.0019 0.0004 0 0.0058 – – – – – –
Bud 0.0005 0.0002 0.0004 0 0.0007 0 0.0002 0.0001 0.0001 0.0004 0 0.0001 – – – – – –
Bud Neck 0.0013 0.0007 0.0007 0.0002 0.0004 0.0006 0.0011 0.0004 0.0053 0.0004 0.0001 0.0002 – – – – – –
Bud Periphery 0.0007 0.0002 0.0004 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0007 0 0.0001 – – – – – –
Bud Site 0.0035 0.0124 0.0029 0.0001 0.0026 0.0002 0.0013 0.0005 0.0006 0.0009 0 0.0002 – – – – – –
Cell Periphery 0.0006 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 – – – – – –
Cytoplasm 0.0043 0.0032 0.0036 0.0006 0.0627 0.0009 0.0039 0.0008 0.0018 0.0005 0.0004 0.0004 – – – – – –
Cytoplasmic Foci 0.0939 0.1012 0.1095 0.0841 0.1247 0.0959 0.1357 0.1804 0.1473 0.0577 0.0837 0.087 – – – – – –
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0.0001 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0018 0.0005 0.002 0.0001 0.004 0.0003 0.0014 0.0002 0.0005 0.0002 0.0001 0.0006 – – – – – –
Endosome 0.4508 0.5478 0.5238 0.5942 0.5121 0.492 0.3875 0.4622 0.3511 0.5854 0.5256 0.3903 – – – – – –
Golgi 0.0564 0.0412 0.0715 0.0469 0.0757 0.0762 0.0596 0.0968 0.1052 0.0753 0.0722 0.0822 – – – – – –
Lipid Particles 0.1879 0.1493 0.1761 0.1909 0.1289 0.2262 0.3138 0.1823 0.3022 0.1745 0.2956 0.3638 – – – – – –
Mitochondria 0.0217 0.0015 0.0054 0.0004 0.0179 0.0068 0.0037 0.0042 0.0118 0.0606 0.0006 0.0073 – – – – – –
None 0.0024 0.0002 0.0004 0 0.0018 0.0001 0.0003 0.0001 0.0088 0.0001 0 0.0001 – – – – – –
Nuclear Periphery 0.024 0.0009 0.0029 0.0003 0.0057 0.0109 0.0014 0.0003 0.0019 0.0002 0.0001 0.001 – – – – – –
Nucleolus 0.0155 0.0116 0.0087 0.002 0.0004 0.0021 0.0006 0.0006 0.0042 0.0006 0.0002 0.0005 – – – – – –
Nucleus 0.0202 0.0032 0.0109 0.0002 0.0052 0.0004 0.0007 0.0001 0.0008 0.0003 0 0.0001 – – – – – –
Peroxisomes 0.031 0.0109 0.0186 0.0059 0.0235 0.0151 0.0492 0.0497 0.0391 0.0097 0.0092 0.0328 – – – – – –
Punctate Nuclear 0.0129 0.0014 0.0008 0.0007 0.0024 0.007 0.0015 0.0013 0.0031 0.0002 0.0006 0.0008 – – – – – –
Vacuole 0.032 0.088 0.0372 0.0572 0.0133 0.0437 0.0211 0.014 0.0091 0.0209 0.0079 0.0136 – – – – – –
Vacuole Periphery 0.0161 0.0249 0.0115 0.0159 0.0136 0.0213 0.0052 0.0059 0.0049 0.0109 0.0036 0.0127 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.105 11.1725 16.8179 19.329 15.0469 14.6325 17.7517 18.0807 17.5683 19.5542
Translational Efficiency 0.6221 0.5996 0.5151 0.4958 0.512 0.6533 0.6308 0.5261 0.5761 0.5865

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1344 604 55 1194 855 1056 2263 1691 2199 1660 2318 2885

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 908.13 961.04 1180.47 1071.86 722.03 897.59 960.02 1002.90 835.77 920.68 965.25 1031.44
Standard Deviation 122.93 155.67 170.50 158.65 306.20 132.41 136.55 145.98 232.21 144.58 141.49 155.12
Intensity Change Log 2 — 0.081698 0.378391 0.239146 — 0.313998 0.411006 0.474047 — 0.189226 0.392928 0.347931

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000120 0.000430 0.000987 0.001355 0.001858 0.000143 0.000066 0.000669 0.000795 0.000248 0.000088 0.000953
Bud Neck 0.012405 0.005113 0.012487 0.003736 0.002934 0.004301 0.003483 0.002579 0.008722 0.004596 0.003696 0.003058
Bud Site 0.007864 0.009558 0.009554 0.017212 0.008965 0.005837 0.004956 0.008616 0.008292 0.007191 0.005065 0.012174
Cell Periphery 0.000225 0.000095 0.000170 0.000164 0.000277 0.000149 0.000170 0.000140 0.000246 0.000129 0.000170 0.000150
Cytoplasm 0.005784 0.003975 0.001374 0.004017 0.005077 0.001659 0.000987 0.002782 0.005509 0.002502 0.000996 0.003293
Cytoplasmic Foci 0.063949 0.095917 0.216832 0.081208 0.116010 0.080080 0.050913 0.088467 0.084191 0.085842 0.054850 0.085463
Eisosomes 0.000066 0.000022 0.000097 0.000023 0.000066 0.000026 0.000027 0.000022 0.000066 0.000025 0.000029 0.000022
Endoplasmic Reticulum 0.000540 0.000232 0.001061 0.000459 0.000378 0.000331 0.000204 0.000232 0.000477 0.000295 0.000224 0.000326
Endosome 0.611645 0.657504 0.324378 0.435974 0.529085 0.695044 0.638054 0.589226 0.579545 0.681385 0.630611 0.525800
Golgi 0.047566 0.056094 0.078471 0.120857 0.199295 0.057268 0.051152 0.107510 0.106560 0.056841 0.051800 0.113034
Lipid Particles 0.009084 0.006608 0.030659 0.002894 0.016024 0.027377 0.011972 0.008037 0.011782 0.019820 0.012415 0.005909
Mitochondria 0.016746 0.008831 0.011051 0.022272 0.043823 0.020739 0.034223 0.024481 0.027274 0.016407 0.033673 0.023567
Mitotic Spindle 0.009580 0.020289 0.029106 0.017090 0.001710 0.005727 0.004377 0.007362 0.006520 0.011025 0.004964 0.011388
None 0.000696 0.000047 0.000093 0.000093 0.003206 0.000195 0.000119 0.000081 0.001672 0.000141 0.000118 0.000086
Nuclear Periphery 0.000695 0.000484 0.001648 0.000416 0.000375 0.000633 0.000956 0.000595 0.000571 0.000579 0.000973 0.000521
Nuclear Periphery Foci 0.009735 0.008891 0.018675 0.002047 0.001612 0.008453 0.005171 0.003632 0.006577 0.008612 0.005491 0.002976
Nucleolus 0.007008 0.000457 0.014612 0.000413 0.000674 0.000973 0.001286 0.000569 0.004545 0.000785 0.001602 0.000504
Nucleus 0.011286 0.000299 0.003988 0.000335 0.002716 0.000632 0.000413 0.000318 0.007954 0.000511 0.000498 0.000325
Peroxisomes 0.022754 0.024948 0.081653 0.012793 0.049416 0.021409 0.011555 0.014261 0.033121 0.022697 0.013219 0.013654
Vacuole 0.127641 0.073436 0.119640 0.202626 0.009813 0.045656 0.125250 0.103803 0.081828 0.055764 0.125117 0.144702
Vacuole Periphery 0.034612 0.026770 0.043464 0.074019 0.006686 0.023367 0.054666 0.036616 0.023754 0.024605 0.054401 0.052096

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.24 -2.68 -2.04 -1.43 -0.56 5.65 5.91 2.40 -1.33 -1.53 3.64 5.88 -0.46 -2.01 -2.54
Bud Neck 3.88 -0.07 5.39 1.16 3.47 -1.00 -0.58 0.33 1.69 2.59 3.29 4.70 5.37 1.97 1.90
Bud Site -0.89 -0.66 -5.34 -3.90 -3.12 1.48 2.22 0.14 -1.88 -3.66 0.96 3.26 -3.36 -4.19 -7.52
Cell Periphery 2.68 1.02 1.22 -2.99 0.17 1.79 1.56 1.90 0.26 1.16 2.79 1.89 2.28 -0.95 1.05
Cytoplasm 1.06 3.92 0.92 -0.36 -4.27 2.54 3.18 1.66 -1.84 -3.89 3.01 5.31 2.11 -1.54 -6.09
Cytoplasmic Foci -5.52 -8.01 -4.05 2.41 7.05 6.60 14.39 5.47 -1.85 -11.10 -0.45 9.69 -0.41 0.10 -10.66
Eisosomes 3.75 -1.18 3.68 -0.34 3.01 4.29 4.27 4.89 2.20 3.32 5.14 4.64 5.50 1.42 3.81
Endoplasmic Reticulum 4.56 -1.22 0.62 -1.78 1.36 0.43 1.42 1.20 1.82 -0.54 2.84 3.78 1.92 -0.51 -1.55
Endosome -3.61 9.89 16.01 17.23 -3.89 -13.18 -9.66 -5.20 10.41 5.74 -11.66 -6.24 6.69 19.18 13.79
Golgi -2.10 -2.36 -13.82 -11.28 -3.20 20.30 22.04 12.56 -12.14 -15.42 12.78 14.79 -1.45 -16.57 -19.31
Lipid Particles 1.48 -2.05 4.31 3.45 2.67 -2.89 1.57 3.05 5.86 2.67 -3.39 -0.36 4.20 6.52 5.32
Mitochondria 3.04 1.12 -1.79 -4.16 -2.04 4.89 2.02 4.19 -1.42 3.63 4.42 -2.26 1.53 -3.46 4.07
Mitotic Spindle -2.62 -1.88 -2.83 0.76 1.23 -3.14 -3.69 -4.99 -1.05 -2.52 -2.43 1.62 -3.38 -0.14 -5.07
None 2.30 2.14 2.16 -3.16 0.02 1.73 1.78 1.80 1.32 3.84 2.21 2.25 2.30 1.03 3.69
Nuclear Periphery 1.52 -2.42 2.93 0.50 3.14 -0.64 -1.48 -0.62 0.18 1.78 -0.01 -1.70 0.35 0.38 2.37
Nuclear Periphery Foci 0.35 -1.40 4.76 3.11 2.38 -3.76 -3.83 -2.11 2.78 1.97 -1.17 0.93 3.41 4.14 3.80
Nucleolus 5.96 -1.01 6.01 0.46 1.82 -0.45 -1.18 0.51 1.36 2.44 5.39 3.94 5.88 1.49 3.42
Nucleus 5.52 2.51 5.48 -0.41 1.96 1.67 1.85 1.93 1.60 0.87 5.65 5.67 5.80 1.31 1.54
Peroxisomes -0.69 -3.10 4.83 3.64 3.64 6.41 9.32 8.47 3.53 -2.19 4.28 9.94 9.62 5.11 -0.40
Vacuole 8.59 0.41 -9.80 -17.67 -3.91 -12.30 -32.87 -25.45 -13.22 4.45 6.73 -9.40 -13.58 -21.93 -4.21
Vacuole Periphery 2.47 -0.72 -10.17 -11.03 -2.60 -7.69 -20.42 -15.17 -5.08 6.54 -0.45 -12.58 -13.21 -11.65 0.88
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
Localization
Cell Percentages endosome (62%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pep5

Pep5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pep5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available