Standard name
Human Ortholog
Description Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.09 0.09 0.07 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.66 0.07 0.67 0.59 0.61 0.28 0.31 0.23 0.1 0.07 0.09 0.17 0.78 0.6 0.64 0.63 0.69 0.65 0.85 0.78 0.84 0.84 0.84 0.86
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.21 0.6 0.31 0.33 0.28 0.6 0.54 0.51 0.67 0.75 0.72 0.61 0 0.06 0 0.1 0.12 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.34 0.39 0.24 0.18 0.19 0.13 0.14 0.12 0.1 0.11 0.08 0.3 0.15 0.18 0.13 0.21 0.24 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.1 0.09 0.12 0.18 0.15 0.14 0.06 0.09 0.18 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.14 0 0.21 0.2 0.19 0.16 0.21 0.28 0.19 0.15 0.17 0.21 0.28 0.31 0.19 0.19 0.18 0.12 0 0 0.05 0.05 0 0
Vac/Vac Membrane 0.29 0.22 0.06 0 0.05 0 0 0 0 0 0 0 0.32 0.34 0.4 0.29 0.27 0.3 0.05 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 1 0 1 2 1 0 3 2 0 0 1 0 0 2 0 1 0
Bud 0 2 4 2 1 13 24 49 32 33 33 59 0 1 2 7 6 3 0 0 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 1 4 1 6 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 9 14 19 15 18 10 7 13 14 14 1 4 7 39 39 21 0 0 0 0 0 1
Endoplasmic Reticulum 0 1 0 1 0 0 2 2 2 1 2 1 0 2 2 19 27 14 0 0 0 0 1 3
Endosome 285 7 236 147 206 116 167 125 35 29 48 104 62 114 105 321 439 221 400 86 299 168 277 295
Golgi 2 1 2 0 1 0 0 0 0 0 0 0 0 1 2 4 7 17 9 4 6 2 4 5
Mitochondria 92 59 107 82 95 250 286 281 233 333 390 378 0 12 3 52 75 35 6 3 5 2 4 9
Nucleus 7 0 0 0 0 1 3 2 3 0 2 2 0 1 6 8 14 2 1 0 0 0 1 0
Nuclear Periphery 17 7 15 2 8 1 4 3 2 6 4 0 4 11 6 7 10 6 4 0 3 1 4 3
Nucleolus 144 33 135 60 60 79 70 77 40 45 60 51 24 28 29 68 132 81 3 0 1 2 2 1
Peroxisomes 4 0 25 24 30 48 95 84 49 26 50 114 0 0 0 8 3 0 1 0 5 3 5 4
SpindlePole 61 2 75 50 65 65 110 151 66 68 89 133 22 58 31 97 117 42 18 3 19 10 8 6
Vac/Vac Membrane 124 22 21 9 18 13 22 17 3 15 14 23 25 64 65 148 174 100 21 7 4 6 12 12
Unique Cell Count 435 98 350 251 336 417 532 546 347 446 539 623 79 189 164 506 638 338 469 111 356 200 328 345
Labelled Cell Count 737 139 629 391 503 602 803 803 476 571 714 886 138 299 260 778 1043 543 469 111 356 200 328 345


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.2 6.7 6.6 6.6 5.2 5.1 5.0 4.8 4.5 4.6 4.9 7.8 7.2 8.0 6.2 7.3 9.1 8.2 8.3 8.9
Std Deviation (1e-4) 0.9 1.6 1.4 1.5 1.8 1.4 1.1 1.2 0.9 0.9 0.9 1.0 0.8 0.8 1.0 1.1 1.4 1.7 1.3 1.2 1.7
Intensity Change (Log2) – – – -0.03 -0.02 -0.37 -0.4 -0.42 -0.49 -0.58 -0.55 -0.45 0.22 0.1 0.27 -0.1 0.13 0.44 0.29 0.32 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.9 2.8 4.8 0 0 0 5.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 2.0 0.8 0.7 -0.8 -0.5 0.3 0 -0.3 0 0 1.0 3.2 2.5 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.7 3.9 3.8
Endosome -2.2 -1.7 -11.0 -10.5 -13.3 0 0 0 -15.9 1.9 -1.7 -0.8 -1.2 0.4 -0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Mitochondria 0.5 -0.7 8.1 6.8 6.2 0 0 0 9.0 -5.7 -6.5 -7.4 -7.5 -7.3 -6.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.5 -1.4 -3.9 -3.5 -3.9 0 0 0 -5.2 0 0.8 -0.3 -2.6 -2.6 -1.9
Nucleolus -3.8 -6.0 -6.0 -8.7 -8.4 0 0 0 -11.6 -1.4 -5.7 -4.7 -8.5 -6.1 -4.1
Peroxisomes 1.1 0.9 2.1 4.5 3.7 0 0 0 4.8 0 -3.8 -3.5 -4.2 -6.0 -5.0
SpindlePole -0.4 -0.7 -2.1 -0.3 2.1 0 0 0 0 1.2 2.4 -0.7 -0.8 -1.2 -3.1
Vacuole -1.3 -0.4 -1.9 -1.3 -2.1 -3.7 -1.8 -2.5 -1.7 6.7 8.5 9.5 8.4 8.0 8.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.022 0.0003 0.0126 0 0.0039 0.0001 0.0114 0.0001 0.0019 0.0004 0 0.0058 – – – – – –
Bud 0.0005 0.0002 0.0004 0 0.0007 0 0.0002 0.0001 0.0001 0.0004 0 0.0001 – – – – – –
Bud Neck 0.0013 0.0007 0.0007 0.0002 0.0004 0.0006 0.0011 0.0004 0.0053 0.0004 0.0001 0.0002 – – – – – –
Bud Periphery 0.0007 0.0002 0.0004 0 0.0004 0.0001 0.0002 0.0001 0.0002 0.0007 0 0.0001 – – – – – –
Bud Site 0.0035 0.0124 0.0029 0.0001 0.0026 0.0002 0.0013 0.0005 0.0006 0.0009 0 0.0002 – – – – – –
Cell Periphery 0.0006 0.0003 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0002 0 0.0001 – – – – – –
Cytoplasm 0.0043 0.0032 0.0036 0.0006 0.0627 0.0009 0.0039 0.0008 0.0018 0.0005 0.0004 0.0004 – – – – – –
Cytoplasmic Foci 0.0939 0.1012 0.1095 0.0841 0.1247 0.0959 0.1357 0.1804 0.1473 0.0577 0.0837 0.087 – – – – – –
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0.0001 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0018 0.0005 0.002 0.0001 0.004 0.0003 0.0014 0.0002 0.0005 0.0002 0.0001 0.0006 – – – – – –
Endosome 0.4508 0.5478 0.5238 0.5942 0.5121 0.492 0.3875 0.4622 0.3511 0.5854 0.5256 0.3903 – – – – – –
Golgi 0.0564 0.0412 0.0715 0.0469 0.0757 0.0762 0.0596 0.0968 0.1052 0.0753 0.0722 0.0822 – – – – – –
Lipid Particles 0.1879 0.1493 0.1761 0.1909 0.1289 0.2262 0.3138 0.1823 0.3022 0.1745 0.2956 0.3638 – – – – – –
Mitochondria 0.0217 0.0015 0.0054 0.0004 0.0179 0.0068 0.0037 0.0042 0.0118 0.0606 0.0006 0.0073 – – – – – –
None 0.0024 0.0002 0.0004 0 0.0018 0.0001 0.0003 0.0001 0.0088 0.0001 0 0.0001 – – – – – –
Nuclear Periphery 0.024 0.0009 0.0029 0.0003 0.0057 0.0109 0.0014 0.0003 0.0019 0.0002 0.0001 0.001 – – – – – –
Nucleolus 0.0155 0.0116 0.0087 0.002 0.0004 0.0021 0.0006 0.0006 0.0042 0.0006 0.0002 0.0005 – – – – – –
Nucleus 0.0202 0.0032 0.0109 0.0002 0.0052 0.0004 0.0007 0.0001 0.0008 0.0003 0 0.0001 – – – – – –
Peroxisomes 0.031 0.0109 0.0186 0.0059 0.0235 0.0151 0.0492 0.0497 0.0391 0.0097 0.0092 0.0328 – – – – – –
Punctate Nuclear 0.0129 0.0014 0.0008 0.0007 0.0024 0.007 0.0015 0.0013 0.0031 0.0002 0.0006 0.0008 – – – – – –
Vacuole 0.032 0.088 0.0372 0.0572 0.0133 0.0437 0.0211 0.014 0.0091 0.0209 0.0079 0.0136 – – – – – –
Vacuole Periphery 0.0161 0.0249 0.0115 0.0159 0.0136 0.0213 0.0052 0.0059 0.0049 0.0109 0.0036 0.0127 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.105 11.1725 16.8179 19.329 15.0469 14.6325 17.7517 18.0807 17.5683 19.5542
Translational Efficiency 0.6221 0.5996 0.5151 0.4958 0.512 0.6533 0.6308 0.5261 0.5761 0.5865

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
Localization
Cell Percentages endosome (62%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pep5

Pep5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pep5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available