Standard name
Human Ortholog
Description Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.11 0.08 0.1 0.07 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.07 0.05 0 0.09 0.06 0.05 0.09 0.14 0.14 0.13 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.93 0.9 0.9 0.85 0.77 0.75 0.7 0.69 0.67 0.63 0.64 0.64 0.84 0.77 0.76 0.71 0.74 0.71 0.89 0.88 0.82 0.58 0.63 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.36 0.46 0.37 0.39 0.38 0.51 0.54 0.53 0.56 0.64 0.55 0.59 0.49 0.41 0.35 0.23 0.26 0.34 0.08 0.09 0.14 0.3 0.27 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 2 0 1 1 0 1 0 0 1 0 0 0 1 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 4 10 6 10 12 12 14 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 6 1 3 7 7 3 4 11 15 4 9 4 13 17 18 19 16 11 4 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Endosome 0 0 0 0 3 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 3 3 9 9 6 16 25 15 33 50 34 40 2 0 0 7 14 2 0 0 0 1 0 0
Nucleus 506 90 120 157 195 139 275 189 237 221 161 198 124 121 161 135 175 103 493 101 109 51 132 124
Nuclear Periphery 0 0 0 0 0 0 2 0 7 2 1 4 0 0 2 0 2 0 0 0 0 0 0 0
Nucleolus 197 46 49 72 95 94 214 147 197 223 137 185 72 64 75 44 62 49 44 9 18 26 55 73
Peroxisomes 2 0 0 0 2 0 0 0 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 1 1 2 1 0 0 3 8 2 2 1 2 0 0 0 1 0
Vac/Vac Membrane 1 1 1 0 1 3 0 0 1 0 0 0 0 0 0 3 4 4 5 2 3 3 12 21
Unique Cell Count 544 100 133 184 253 185 393 275 354 349 251 311 148 157 212 190 235 146 556 115 134 88 209 226
Labelled Cell Count 716 141 182 245 310 259 535 369 502 516 355 447 211 207 269 211 278 170 556 115 134 88 209 226


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 21.0 9.7 11.4 11.2 9.7 9.1 9.3 8.6 8.5 8.4 8.6 15.9 16.1 15.7 18.9 19.4 17.8 15.4 19.3 20.2
Std Deviation (1e-4) 7.9 4.6 3.6 4.4 3.7 3.3 3.4 3.5 3.5 2.6 3.9 3.2 5.0 5.9 5.3 7.6 6.6 7.1 5.2 7.6 6.5
Intensity Change (Log2) – – – 0.23 0.21 -0.01 -0.1 -0.07 -0.17 -0.2 -0.21 -0.17 0.71 0.72 0.69 0.95 0.99 0.87 0.66 0.99 1.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.4 2.9 2.4 2.7 1.9 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.7 -2.1 0.6 -0.2 -0.5 0.9 2.3 2.0 1.9 -2.3 0 0 -1.3 -0.3 -2.3
Nucleus -1.3 -3.2 -3.4 -4.7 -4.7 -5.2 -5.8 -5.5 -5.7 -1.6 -3.0 -3.3 -4.2 -3.6 -4.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.1 2.5 3.5 3.1 3.7 5.4 3.3 4.4 2.0 0.7 -0.3 -2.7 -2.1 -0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.0683 11.2628 10.655 10.518 9.6226 9.9916 9.5667 10.5561 9.945 9.08 7.1323 9.2901 13.6651 13.3545 12.6796 11.6588 11.6839 12.261
Actin 0.0084 0.0002 0.0011 0.0007 0.0002 0 0.0244 0.0001 0.0032 0.03 0.064 0.011 0.0068 0 0.0076 0.0014 0.0004 0.0002
Bud 0.0004 0.0002 0.0001 0.0001 0.0002 0 0.0004 0.0034 0.0001 0.0006 0.0005 0.0001 0.0001 0 0.0001 0.0005 0.0002 0
Bud Neck 0.0015 0.0002 0.0001 0 0.0001 0.0004 0.0005 0 0 0.0001 0.0007 0.001 0.0003 0 0 0.001 0.0005 0.0003
Bud Periphery 0.0004 0.0002 0 0.0001 0.0004 0 0.0006 0.0004 0.0001 0.0014 0.0006 0.0002 0.0002 0 0.0001 0.001 0.0005 0
Bud Site 0.0029 0.0006 0.0003 0.0002 0.0005 0.0001 0.0011 0.0048 0.0003 0.001 0.0017 0.0002 0.0006 0 0.0001 0.0003 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0 0 0.0003 0 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0001 0.0004 0
Cytoplasm 0.0085 0.0002 0.0003 0.0031 0.001 0.0001 0.0045 0.0007 0.0001 0.0003 0.003 0.0001 0.0002 0 0 0.0006 0.0004 0.0002
Cytoplasmic Foci 0.0102 0.0002 0.007 0.0138 0.0052 0.0018 0.0277 0.0002 0.0013 0.0003 0.0502 0.0034 0.0065 0 0.0001 0.0023 0.0022 0.0001
Eisosomes 0.0005 0 0 0 0.0001 0 0.0002 0 0 0.0004 0.0002 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0024 0.0002 0.0001 0.0009 0.0001 0.0001 0.0013 0 0 0.0008 0.0022 0.0001 0.0002 0 0 0.0008 0.0007 0.0001
Endosome 0.0061 0.002 0.0019 0.0071 0.0002 0.0075 0.0124 0 0.0004 0.0015 0.0204 0.004 0.0015 0 0 0.0009 0.0018 0.0008
Golgi 0.0016 0.0003 0.0012 0.0045 0.0001 0.0008 0.0083 0 0.0012 0.0026 0.0234 0.0033 0.0008 0 0.0001 0.0009 0.0008 0
Lipid Particles 0.0028 0.0001 0.0027 0.0091 0.0029 0.0026 0.0253 0 0.0018 0.0002 0.1166 0.0021 0.0074 0 0.0001 0.0154 0.0229 0.0001
Mitochondria 0.0012 0.0016 0.0007 0.0216 0.0084 0.0005 0.0083 0.0004 0.0019 0.0185 0.0144 0.0007 0.0017 0 0.0008 0.0019 0.0191 0.0001
None 0.0058 0 0.0002 0.0028 0.0027 0.0001 0.0023 0.0005 0.0001 0.0002 0.0021 0.0001 0.0005 0 0 0.0001 0.0004 0.0001
Nuclear Periphery 0.0047 0.0013 0.0009 0.0087 0.002 0.0004 0.0086 0.0002 0.0003 0.0004 0.0075 0.0005 0.0008 0.0003 0.0001 0.0045 0.0028 0.0025
Nucleolus 0.0669 0.0332 0.0359 0.0294 0.3352 0.2181 0.0841 0.0258 0.0309 0.0396 0.2305 0.2323 0.0865 0.0333 0.0308 0.0161 0.3623 0.2195
Nucleus 0.8569 0.9579 0.9317 0.8853 0.6339 0.7659 0.7433 0.963 0.9544 0.9014 0.3919 0.7361 0.8807 0.9662 0.9593 0.9412 0.5693 0.7745
Peroxisomes 0.0041 0 0.0045 0.0021 0.0005 0.0005 0.0177 0 0.0017 0.0001 0.039 0.0034 0.0012 0 0.0003 0.0093 0.0068 0
Punctate Nuclear 0.0134 0.0002 0.0113 0.0088 0.0031 0.0006 0.0246 0.0002 0.0016 0.0001 0.0289 0.0009 0.0029 0.0001 0 0.0004 0.0038 0.0011
Vacuole 0.0006 0.0009 0.0001 0.0007 0.0009 0.0002 0.0018 0 0.0002 0.0002 0.0009 0.0003 0.0004 0 0.0002 0.0009 0.0018 0.0001
Vacuole Periphery 0.0004 0.0005 0.0001 0.0009 0.002 0.0002 0.0025 0 0.0003 0.0002 0.0012 0.0001 0.0004 0 0.0002 0.0006 0.0023 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.7047 43.7002 29.3391 60.3362 39.2362 48.8579 57.8616 41.8929 61.8968 59.1575
Translational Efficiency 1.5911 1.7225 2.5508 1.1913 2.3936 1.9554 1.4975 2.5153 1.5701 1.5682

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1092 1685 1139 1431 2209 1618 958 1342 3301 3303 2097 2773

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 962.83 1446.39 1655.44 1650.55 1217.38 1442.48 1550.69 1650.99 1133.17 1444.47 1607.59 1650.76
Standard Deviation 225.68 303.41 298.77 343.77 253.90 273.90 283.98 338.00 272.64 289.34 296.73 340.99
Intensity Change Log 2 — 0.587104 0.781862 0.777594 — 0.244772 0.349131 0.439552 — 0.406038 0.556366 0.598672

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000429 0.000517 0.000814 0.000899 0.000460 0.000515 0.000742 0.000795 0.000450 0.000516 0.000781 0.000849
Bud Neck 0.002701 0.003478 0.012955 0.016030 0.003282 0.004289 0.010160 0.014670 0.003090 0.003875 0.011678 0.015372
Bud Site 0.000533 0.000520 0.001095 0.002798 0.000539 0.001287 0.001166 0.001674 0.000537 0.000896 0.001127 0.002254
Cell Periphery 0.000167 0.000034 0.000038 0.000115 0.000036 0.000208 0.000032 0.000095 0.000080 0.000119 0.000035 0.000105
Cytoplasm 0.002795 0.000141 0.000037 0.000592 0.000232 0.000112 0.000519 0.000225 0.001080 0.000127 0.000257 0.000415
Cytoplasmic Foci 0.000238 0.000572 0.000117 0.000549 0.000321 0.000210 0.000859 0.000335 0.000293 0.000395 0.000456 0.000446
Eisosomes 0.000099 0.000198 0.000154 0.000057 0.000182 0.000201 0.000125 0.000070 0.000155 0.000199 0.000141 0.000064
Endoplasmic Reticulum 0.000996 0.000484 0.000466 0.000603 0.000589 0.000672 0.000432 0.000534 0.000723 0.000576 0.000450 0.000570
Endosome 0.000097 0.000252 0.000017 0.001093 0.000035 0.000097 0.000397 0.000546 0.000055 0.000176 0.000191 0.000829
Golgi 0.000040 0.000293 0.000071 0.000340 0.000092 0.000211 0.000296 0.000104 0.000075 0.000253 0.000174 0.000226
Lipid Particles 0.000131 0.000121 0.000010 0.000263 0.000163 0.000179 0.000334 0.000039 0.000153 0.000149 0.000158 0.000155
Mitochondria 0.000576 0.000725 0.001861 0.003451 0.000733 0.001031 0.002391 0.003158 0.000681 0.000875 0.002103 0.003309
Mitotic Spindle 0.000303 0.001805 0.000150 0.009881 0.000793 0.002439 0.000514 0.005663 0.000631 0.002116 0.000316 0.007840
None 0.004492 0.000507 0.000651 0.001856 0.000838 0.000701 0.000740 0.001028 0.002047 0.000602 0.000692 0.001456
Nuclear Periphery 0.000029 0.000046 0.000051 0.000603 0.000020 0.000069 0.000067 0.000252 0.000023 0.000057 0.000058 0.000433
Nuclear Periphery Foci 0.000151 0.000480 0.000009 0.000486 0.000123 0.000426 0.000112 0.000143 0.000132 0.000454 0.000056 0.000320
Nucleolus 0.075343 0.067175 0.084089 0.038649 0.075365 0.073838 0.073772 0.038751 0.075358 0.070439 0.079376 0.038698
Nucleus 0.909195* 0.919114* 0.890637* 0.918375* 0.913252* 0.910587* 0.902399* 0.929339* 0.911910* 0.914937* 0.896010* 0.923681*
Peroxisomes 0.001113 0.002837 0.006315 0.001464 0.002455 0.002140 0.004311 0.001805 0.002011 0.002496 0.005399 0.001629
Vacuole 0.000329 0.000401 0.000262 0.000828 0.000232 0.000474 0.000350 0.000512 0.000264 0.000437 0.000302 0.000675
Vacuole Periphery 0.000242 0.000300 0.000202 0.001066 0.000256 0.000314 0.000282 0.000261 0.000252 0.000307 0.000238 0.000677

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.63 -13.75 -7.73 -5.53 -1.79 -1.22 -6.70 -5.97 -5.08 -1.34 -1.78 -13.00 -9.48 -7.50 -2.18
Bud Neck -3.39 -19.15 -12.66 -11.95 -3.51 -3.00 -13.91 -11.21 -10.12 -4.59 -3.82 -23.06 -16.75 -15.64 -5.57
Bud Site -0.64 -4.84 -4.07 -3.94 -3.20 -1.26 -1.66 -3.68 -0.75 -0.74 -1.34 -3.03 -5.11 -3.52 -3.10
Cell Periphery 2.05 1.98 0.51 -3.79 -3.43 -1.19 0.47 -2.66 0.82 -3.00 -0.55 1.95 -1.20 0.13 -4.48
Cytoplasm 1.95 2.37 1.59 -1.23 -4.40 1.14 -0.91 0.13 -2.17 0.96 1.94 1.25 1.25 -1.59 -0.39
Cytoplasmic Foci -1.66 0.71 -1.33 0.87 -2.49 0.31 -0.95 0.09 -0.27 1.01 -1.17 -0.61 -0.75 0.68 0.19
Eisosomes -6.31 -6.75 5.32 9.60 15.93 -1.14 4.89 11.08 9.49 9.93 -3.75 1.18 12.55 13.51 18.51
Endoplasmic Reticulum 5.01 5.27 2.86 -1.85 -1.68 -1.20 3.19 0.36 1.39 -2.66 2.34 5.25 1.72 -0.68 -2.58
Endosome -1.40 1.40 -2.48 -1.85 -2.75 -1.54 -1.31 -1.61 -1.33 -0.31 -2.05 -1.03 -3.01 -2.28 -2.20
Golgi -1.78 -6.70 -2.80 -0.21 -2.42 -0.87 -1.01 -0.19 0.84 0.98 -1.66 -1.08 -2.33 0.45 -0.59
Lipid Particles 0.01 0.95 -0.60 -0.75 -1.76 -0.18 -0.66 0.95 1.36 1.42 -0.06 0.04 0.09 0.18 0.04
Mitochondria -2.71 -17.33 -7.95 -7.54 -4.20 -1.13 -2.00 -3.68 -3.03 -0.83 -1.42 -3.92 -7.14 -6.33 -2.39
Mitotic Spindle -2.12 0.44 -5.12 -4.13 -5.21 -1.95 -0.17 -3.22 -1.78 -3.13 -2.65 0.35 -5.94 -4.39 -6.09
None 4.03 3.85 2.21 -3.02 -2.66 0.50 0.10 -0.97 -2.11 -1.57 3.87 3.49 0.99 -3.55 -3.05
Nuclear Periphery -1.03 -3.18 -4.52 -4.25 -4.31 -2.76 -2.73 -6.71 -4.84 -4.94 -2.46 -4.17 -5.98 -5.34 -5.44
Nuclear Periphery Foci -1.25 1.77 -2.31 0.05 -3.42 -1.55 0.45 0.14 1.64 -0.35 -1.83 1.50 -2.01 0.76 -3.21
Nucleolus 1.87 -2.28 7.58 6.29 9.63 0.39 -0.08 9.45 8.29 7.32 1.73 -1.89 12.18 10.30 12.09
Nucleus -1.64 4.17 0.49 1.96 -3.25 0.75 2.73 -2.07 -2.51 -4.04 -0.60 5.32 -0.70 -0.18 -5.22
Peroxisomes -7.63 -11.70 -3.34 4.88 10.36 0.63 -3.46 1.06 0.72 4.38 -1.69 -8.78 0.82 3.05 10.21
Vacuole -0.82 0.18 -3.16 -2.46 -3.45 -1.43 -0.89 -1.68 -0.04 -0.91 -1.72 -0.71 -3.69 -1.92 -3.34
Vacuole Periphery -0.78 0.56 -1.73 -1.57 -1.82 -1.28 -0.19 -0.15 1.13 0.12 -1.38 0.25 -1.73 -1.41 -1.76
Standard name
Human Ortholog
Description Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Taf9

Taf9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Taf9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available