Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.98 0.98 0.98 0.95 0.93 0.86 0.81 0.79 0.75 0.76 0.69 0.87 0.92 0.98 0.99 0.97 0.99 0.96 0.89 0.85 0.68 0.6 0.63
Endoplasmic Reticulum 0 0 0 0 0.06 0.07 0.11 0.14 0.18 0.17 0.12 0.22 0.13 0.09 0 0 0 0 0 0 0.1 0.21 0.31 0.26
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 4 12 4 4 9 11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 0 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 4 1 8 6 8 14 6 10 16 16 0 2 0 2 6 3 0 0 0 0 0 0
Cytoplasm 102 99 276 184 225 283 303 331 216 214 198 230 60 102 103 297 354 251 97 107 225 76 111 138
Endoplasmic Reticulum 0 4 7 2 14 20 40 57 50 48 30 72 9 10 3 11 14 11 0 1 26 23 58 57
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0
Golgi 0 0 1 1 0 0 6 13 3 20 13 21 0 0 0 0 0 1 0 0 1 1 1 1
Mitochondria 0 0 0 0 0 0 2 4 0 7 22 10 0 0 0 0 1 2 0 0 0 0 3 2
Nucleus 0 0 0 1 1 0 0 3 0 0 0 1 0 0 1 0 0 0 1 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 2 0 1 0 3 0 0 0 1 2 1 0 3 1 6 1 12
Unique Cell Count 102 101 283 188 237 305 353 411 273 285 260 332 69 111 105 300 364 254 101 120 264 112 186 221
Labelled Cell Count 102 103 288 189 248 309 365 436 280 304 288 364 69 114 107 312 382 269 101 120 264 112 186 221


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 112.1 63.3 104.3 87.0 71.0 54.8 50.6 49.8 48.4 45.4 42.3 43.2 91.3 96.8 96.7 114.0 111.9 112.2 83.8 85.9 89.7
Std Deviation (1e-4) 20.7 26.6 21.4 21.7 12.2 11.8 11.2 11.9 11.0 11.8 11.5 11.4 22.3 23.1 19.4 24.7 26.6 26.1 20.8 22.8 25.2
Intensity Change (Log2) – – – -0.26 -0.56 -0.93 -1.04 -1.07 -1.11 -1.2 -1.3 -1.27 -0.19 -0.11 -0.11 0.13 0.1 0.11 -0.32 -0.28 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.5 0 0.8 1.6 0 1.6 2.9 2.4 0 0 0 0 0 0
Cytoplasm 0.2 -1.6 -2.7 -5.1 -6.6 -6.8 -7.8 -7.5 -9.1 -3.8 -2.5 0.3 1.4 -0.2 1.1
Endoplasmic Reticulum 0 2.0 2.4 4.2 5.1 6.2 5.8 4.2 7.1 0 0 0 0.8 1.0 1.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 2.6 0 4.2 3.4 4.0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 5.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0132 0.0009 0.0065 0.0015 0.0008 0.0024 0.0083 0.0005 0.0016 0.0004 0.0004 0.0009 – – – – – –
Bud 0.0044 0.001 0.0018 0.0028 0.0017 0.0016 0.0026 0.0009 0.0034 0.0034 0.0027 0.0015 – – – – – –
Bud Neck 0.0012 0.0007 0.0015 0.0022 0.0013 0.0013 0.0018 0.0008 0.0011 0.0018 0.0007 0.0014 – – – – – –
Bud Periphery 0.0054 0.0006 0.0013 0.0023 0.0006 0.0011 0.0047 0.0008 0.0067 0.0018 0.0013 0.0015 – – – – – –
Bud Site 0.0016 0.0008 0.0034 0.0053 0.0002 0.0006 0.0021 0.001 0.0011 0.0005 0.0006 0.0003 – – – – – –
Cell Periphery 0.0435 0.0163 0.0174 0.0208 0.0025 0.0328 0.0486 0.0197 0.0311 0.0064 0.011 0.0463 – – – – – –
Cytoplasm 0.5307 0.8409 0.6542 0.5334 0.7135 0.8526 0.4909 0.8404 0.7156 0.5707 0.6387 0.6508 – – – – – –
Cytoplasmic Foci 0.0177 0.0034 0.0126 0.0075 0.0067 0.0111 0.0303 0.0017 0.0093 0.0042 0.0136 0.0022 – – – – – –
Eisosomes 0.0004 0 0.0002 0.0001 0 0.0002 0.0007 0 0.0001 0 0.0001 0.0004 – – – – – –
Endoplasmic Reticulum 0.0544 0.0304 0.0348 0.014 0.0054 0.0062 0.0619 0.0245 0.0331 0.003 0.0022 0.0141 – – – – – –
Endosome 0.0215 0.0133 0.0351 0.0459 0.0192 0.0215 0.0184 0.0039 0.0089 0.0225 0.0112 0.0142 – – – – – –
Golgi 0.0052 0.0079 0.0101 0.0084 0.0017 0.0038 0.0047 0.0007 0.0017 0.0016 0.0008 0.0052 – – – – – –
Lipid Particles 0.0059 0.0012 0.0067 0.0064 0.0013 0.0041 0.01 0.0015 0.0024 0.0015 0.0047 0.008 – – – – – –
Mitochondria 0.007 0.0075 0.0058 0.0096 0.0009 0.002 0.0106 0.0006 0.0015 0.0006 0.0108 0.0133 – – – – – –
None 0.0078 0.0003 0.0212 0.0008 0.0004 0.0008 0.0127 0.0004 0.0093 0.0002 0.0127 0.0015 – – – – – –
Nuclear Periphery 0.0466 0.0037 0.0193 0.0084 0.0095 0.0104 0.0419 0.0055 0.0096 0.0079 0.0056 0.0239 – – – – – –
Nucleolus 0.0019 0.0001 0.0029 0.0008 0.0007 0.0019 0.0048 0.0006 0.0012 0.0007 0.0131 0.0029 – – – – – –
Nucleus 0.0107 0.0019 0.0047 0.0045 0.0065 0.0049 0.0111 0.0032 0.0038 0.0071 0.0117 0.0103 – – – – – –
Peroxisomes 0.0007 0.0001 0.0018 0.0015 0.0002 0.0003 0.0039 0.0001 0.0006 0.0001 0.0009 0.0001 – – – – – –
Punctate Nuclear 0.0056 0 0.0018 0.0002 0.0001 0.0002 0.002 0.0001 0.0004 0.0001 0.0005 0.0002 – – – – – –
Vacuole 0.1632 0.057 0.1268 0.2438 0.163 0.0314 0.1821 0.085 0.1332 0.2714 0.1928 0.1285 – – – – – –
Vacuole Periphery 0.0516 0.0118 0.0301 0.08 0.0638 0.0091 0.0461 0.0081 0.0244 0.0941 0.0639 0.0726 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1980.3877 1970.8518 2232.4682 2019.7733 2129.3793 1874.1117 1732.8263 1636.7737 1690.4859 1663.5731
Translational Efficiency 1.6351 1.673 1.5688 1.4677 1.7164 1.7596 1.687 1.8229 1.5909 1.8972

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2595 2225 2495 1583 2016 2278 3412 3196 4611 4503 5907 4779

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5021.03 5806.89 6639.18 5055.50 5164.96 5833.60 7147.82 6740.38 5083.96 5820.40 6932.98 6182.28
Standard Deviation 1152.64 1454.87 1557.82 1831.98 1120.97 1443.91 1399.31 1331.96 1141.13 1449.40 1489.69 1710.85
Intensity Change Log 2 — 0.209782 0.403022 0.009870 — 0.175629 0.468746 0.384073 — 0.192566 0.436722 0.211697

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000039 0.000084 0.000127 0.000306 0.000055 0.000125 0.000055 0.000043 0.000046 0.000105 0.000086 0.000130
Bud Neck 0.000093 0.000201 0.001107 0.002438 0.000159 0.000314 0.000171 0.000108 0.000122 0.000258 0.000567 0.000880
Bud Site 0.001749 0.003656 0.006181 0.007376 0.001745 0.004232 0.002404 0.001958 0.001747 0.003947 0.003999 0.003753
Cell Periphery 0.036017 0.045885 0.031255 0.100125* 0.052543 0.035042 0.049281 0.074838 0.043242 0.040400 0.041667 0.083214
Cytoplasm 0.384580* 0.316306* 0.319409* 0.215210* 0.312473* 0.344622* 0.463258* 0.486716* 0.353053* 0.330631* 0.402499* 0.396782*
Cytoplasmic Foci 0.027397 0.018236 0.006678 0.002872 0.019627 0.019083 0.011132 0.005880 0.024000 0.018664 0.009251 0.004884
Eisosomes 0.000002 0.000003 0.000003 0.000128 0.000003 0.000003 0.000002 0.000002 0.000002 0.000003 0.000003 0.000043
Endoplasmic Reticulum 0.013662 0.009932 0.005513 0.135367* 0.011912 0.006968 0.009654 0.007397 0.012897 0.008432 0.007905 0.049786
Endosome 0.001722 0.001854 0.001424 0.004873 0.001581 0.001837 0.001126 0.000778 0.001660 0.001845 0.001252 0.002134
Golgi 0.098138 0.090686 0.035412 0.025095 0.103438 0.109953 0.030016 0.008492 0.100455 0.100433 0.032295 0.013991
Lipid Particles 0.031041 0.032956 0.020482 0.029712 0.038842 0.029453 0.019294 0.011617 0.034452 0.031184 0.019796 0.017611
Mitochondria 0.000502 0.000680 0.000400 0.001048 0.000524 0.000753 0.000723 0.000698 0.000511 0.000717 0.000586 0.000814
Mitotic Spindle 0.000938 0.000930 0.000785 0.000432 0.000700 0.000989 0.000439 0.000203 0.000834 0.000960 0.000585 0.000279
None 0.001199 0.001525 0.000942 0.001609 0.001239 0.001577 0.000775 0.000493 0.001217 0.001551 0.000846 0.000863
Nuclear Periphery 0.000882 0.000939 0.000885 0.003128 0.001202 0.000959 0.000649 0.000477 0.001022 0.000950 0.000749 0.001355
Nuclear Periphery Foci 0.009693 0.011569 0.009325 0.014608 0.011486 0.011502 0.005511 0.002190 0.010477 0.011535 0.007122 0.006303
Nucleolus 0.000093 0.000131 0.000211 0.000268 0.000254 0.000106 0.000140 0.000209 0.000164 0.000118 0.000170 0.000229
Nucleus 0.001107 0.001344 0.004715 0.006984 0.001160 0.001637 0.001197 0.001184 0.001130 0.001492 0.002683 0.003105
Peroxisomes 0.000219 0.000243 0.000109 0.000094 0.000213 0.000107 0.000621 0.000971 0.000216 0.000174 0.000405 0.000681
Vacuole 0.147480 0.209996* 0.396694* 0.333326* 0.183874 0.206562* 0.247422* 0.295697* 0.163392 0.208259* 0.310472* 0.308161*
Vacuole Periphery 0.243447* 0.252845* 0.158344* 0.115002* 0.256970* 0.224178* 0.156128* 0.100050* 0.249360* 0.238343* 0.157064* 0.105003*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.13 -8.12 -8.23 -6.00 -4.90 -2.44 -0.37 2.01 2.82 3.24 -3.63 -7.28 -7.47 -0.87 -3.22
Bud Neck -5.15 -9.48 -10.12 -9.64 -3.33 -3.59 -1.49 1.53 4.08 2.67 -5.34 -9.64 -9.78 -7.47 -1.40
Bud Site -5.38 -8.04 -5.21 -3.44 -0.86 -5.40 -4.17 -1.23 4.79 2.21 -7.49 -8.78 -5.26 0.22 0.66
Cell Periphery -4.58 3.71 -17.11 -13.71 -18.97 8.04 1.57 -8.16 -16.33 -10.23 2.02 1.71 -19.02 -20.26 -20.52
Cytoplasm 7.32 7.33 17.11 10.04 10.50 -3.28 -16.64 -19.32 -15.85 -3.04 3.31 -7.51 -6.48 -9.72 0.60
Cytoplasmic Foci 5.78 15.52 16.97 12.70 3.97 0.40 6.82 11.64 11.85 8.82 4.77 15.76 20.59 17.33 9.10
Eisosomes -5.70 -6.04 -2.97 -2.94 -2.94 -0.21 3.36 5.34 6.08 4.21 -3.91 -1.66 -2.92 -2.87 -2.91
Endoplasmic Reticulum 3.60 8.76 -15.92 -16.47 -17.14 5.58 2.52 5.53 -0.73 3.66 6.48 7.58 -13.43 -15.31 -15.59
Endosome -1.53 3.10 -8.61 -8.17 -9.27 -3.24 7.11 13.55 15.31 8.26 -3.17 7.71 -3.73 -2.17 -6.75
Golgi 2.25 23.83 27.52 25.15 6.40 -1.55 25.56 34.80* 36.70* 20.73* 0.18 35.07 46.23* 45.94* 21.27*
Lipid Particles -1.01 7.28 0.56 1.44 -5.59 4.69 11.29 16.50 13.16 9.17 2.48 13.18 14.44 12.10 2.40
Mitochondria -1.80 1.33 -4.60 -2.93 -5.54 -2.31 -2.98 -2.73 0.86 0.61 -2.93 -1.76 -5.39 -1.29 -4.53
Mitotic Spindle -0.48 0.32 1.55 1.52 2.38 -4.93 7.61 23.62* 13.10 9.10 -1.11 1.60 3.97 4.04 6.17
None -1.08 1.82 -2.29 -0.44 -3.07 -1.13 2.53 4.91 3.88 2.12 -1.60 3.33 3.15 3.09 -0.28
Nuclear Periphery -1.22 -1.35 -11.69 -11.30 -11.13 3.12 10.05 13.63 7.96 6.13 1.56 6.61 -4.68 -5.39 -8.09
Nuclear Periphery Foci -3.36 -0.35 -4.33 -2.75 -4.06 -0.23 12.76 19.16* 16.74 9.99 -2.66 8.86 7.74 9.17 1.84
Nucleolus -4.51 -6.11 -10.48 -7.85 -1.81 0.94 0.74 0.29 -10.59 -12.96 0.65 -0.21 -1.02 -13.08 -4.72
Nucleus -0.78 -8.18 -6.98 -6.92 -1.15 -2.40 -0.80 -1.32 1.48 -0.82 -2.06 -7.77 -6.71 -5.73 -0.03
Peroxisomes -0.55 3.78 4.11 4.90 0.79 3.20 -6.19 -9.44 -11.58 -3.75 1.63 -4.60 -8.51 -9.69 -4.62
Vacuole -12.13 -40.58 -25.87 -16.21 8.04 -4.13 -11.65 -19.04 -14.51 -8.22 -11.78 -35.93 -33.07 -21.39 1.00
Vacuole Periphery -1.47 19.26 24.49* 24.61* 8.09 5.80 19.71 32.47* 28.37* 17.15* 2.96 27.41 42.51 39.12* 18.74*
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl20a

Rpl20a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl20a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available