Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 0.18 0.18 0.09 0.14 0.1 0 0 0 0 0 0.08 0 0 0 0 0 0.15 0.11 0.15 0.18 0.22 0.18
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.18 0.22 0.39 0.2 0.31 0.2 0 0 0 0 0 0 0.17 0.06 0 0.08 0.08 0.05 0.11 0 0.15 0.09 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.2 0.3 0.13 0.17 0.15
Golgi 0.33 0.56 0.58 0.71 0.67 0.58 0.42 0.48 0.07 0.2 0.15 0.23 0.49 0.42 0.23 0.65 0.67 0.68 0.47 0.51 0.28 0.43 0.39 0.44
Mitochondria 0.19 0.17 0 0.05 0 0.14 0.45 0.32 0.82 0.72 0.79 0.59 0.22 0.25 0.37 0.14 0.07 0.17 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 0.24 0.1 0.12 0.11 0.15 0.3 0.33 0.3 0.26 0.26 0.3 0.31 0.48 0.56 0.37 0.36 0.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0.09 0.14 0.16
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0.07 0.07 0.06 0.09 0 0.07 0 0.06 0 0.08 0 0 0.05 0.12 0.06 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 23 74 70 39 73 76 36 23 18 10 14 18 12 9 3 0 2 48 30 70 34 86 68
Bud 5 4 10 8 5 7 9 9 5 5 3 5 6 1 1 1 0 0 0 1 5 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 7 2 3 0 2 1 0 0 1 1 1 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 7 6 12 12 16 10 10 12 4 8 10 8 1 6 6 6 3 2 0 2 8 1 0 0
Endoplasmic Reticulum 39 53 164 76 140 105 32 5 2 14 12 5 37 16 8 15 12 5 33 10 72 16 13 7
Endosome 0 0 1 3 0 2 2 0 3 1 0 2 1 0 1 0 0 0 49 56 143 24 68 56
Golgi 74 134 245 274 302 306 328 422 44 172 135 203 110 119 72 128 96 71 147 144 134 82 155 164
Mitochondria 43 40 6 21 8 72 348 282 555 630 724 524 48 69 117 28 10 18 1 0 3 0 1 3
Nucleus 0 0 0 0 0 0 0 2 2 2 2 10 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 1 2 5 4 7 15 25 17 0 0 0 0 0 0 1 3 7 0 1 1
Nucleolus 97 58 40 48 50 81 237 290 206 229 241 265 70 135 177 73 52 42 0 1 0 0 0 1
Peroxisomes 0 1 0 0 0 0 0 3 0 1 5 0 0 0 0 0 0 0 19 15 16 17 56 60
SpindlePole 1 0 1 1 1 0 0 2 0 1 0 2 0 0 0 0 0 0 6 6 9 4 3 5
Vac/Vac Membrane 4 12 28 27 27 46 29 59 21 50 23 68 1 8 17 23 8 10 1 4 6 3 3 2
Unique Cell Count 222 241 419 387 449 529 779 871 676 878 912 887 223 281 316 197 143 104 314 281 484 193 397 375
Labelled Cell Count 277 331 589 542 592 704 1078 1127 872 1146 1193 1124 293 366 409 277 181 150 314 281 484 193 397 375


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 45.1 42.4 35.1 34.6 34.0 31.1 30.1 31.4 28.0 30.6 27.5 29.4 47.6 45.5 45.0 71.9 72.1 67.1 41.6 41.4 42.6
Std Deviation (1e-4) 9.7 9.0 6.7 7.1 6.6 6.0 6.4 7.6 6.5 9.5 8.8 9.5 9.2 10.5 9.9 20.9 20.0 18.3 8.7 9.1 10.1
Intensity Change (Log2) – – – -0.02 -0.04 -0.17 -0.22 -0.16 -0.33 -0.2 -0.35 -0.26 0.44 0.38 0.36 1.03 1.04 0.94 0.25 0.24 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -3.9 -1.6 -3.9 -8.1 -8.1 -10.2 -11.5 -10.8 -3.3 -5.3 -6.3 -5.6 -5.4 -4.1
Bud -0.3 -1.4 -1.2 -1.6 -1.9 -2.3 0 0 0 0.2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0.6 -1.0 -1.9 -1.9 -3.0 -2.7 -2.3 -2.7 0 -0.6 -0.8 0.1 0 0
Endoplasmic Reticulum -6.0 -2.5 -6.5 -15.6 -19.2 -17.4 -18.4 -18.9 -19.4 -5.9 -9.9 -11.6 -8.0 -6.8 -6.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 3.7 2.7 -0.2 -5.4 -3.4 -19.0 -14.0 -16.4 -12.6 -2.2 -4.2 -9.7 1.5 1.8 1.8
Mitochondria 3.1 0.4 6.8 15.6 12.5 26.0 23.7 26.5 19.8 8.7 9.7 12.8 6.5 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2.2 3.1 2.4 0 0 0 0 0 0
Nucleolus 1.3 0.8 2.6 8.2 9.2 8.1 6.9 7.0 8.1 7.0 11.5 13.7 8.2 7.5 7.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.2 -0.4 1.1 -2.3 0.1 -2.8 -0.7 -3.7 0.6 -3.6 -2.3 -0.7 2.1 -0.5 1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.44 32.855 29.6751 28.7229 24.3076 31.3537 37.8464 42.431 39.8078 38.2886 35.2203 39.562 30.2762 42.1382 39.7067 41.0374 25.4427 38.3103
Actin 0.0108 0.0077 0.0113 0.0009 0.0369 0.0154 0.0163 0.0057 0.0178 0.0061 0.0034 0.0021 0.0361 0.0179 0.0337 0.0061 0.1639 0.0103
Bud 0.0004 0.0001 0.0001 0.0001 0.001 0.0002 0.0003 0 0.0001 0 0 0 0.0014 0.0003 0.0005 0 0.0006 0.0001
Bud Neck 0.0009 0.0002 0.0003 0.0001 0.0009 0.0007 0.0003 0.0001 0.0004 0.0002 0.0001 0.0001 0.0011 0.0025 0.0004 0.0001 0.0003 0.0004
Bud Periphery 0.0006 0.0001 0.0002 0.0003 0.0017 0.0004 0.0004 0 0.0001 0 0 0 0.0022 0.0005 0.0006 0 0.0014 0.0001
Bud Site 0.002 0.0006 0.0005 0.0006 0.0038 0.0001 0.0018 0.0001 0.0008 0.0001 0 0 0.0093 0.0037 0.0007 0 0.0008 0.0001
Cell Periphery 0.0014 0.0012 0.0007 0.0007 0.0004 0.0007 0.0017 0.0005 0.0011 0.0004 0.0003 0.0005 0.0016 0.0019 0.0016 0.0002 0.0004 0.0005
Cytoplasm 0.0032 0.0005 0.0028 0.0007 0.0007 0.0005 0.0009 0.0002 0.0013 0.0002 0.0006 0.0006 0.0049 0.0008 0.0182 0.0002 0.0071 0.0038
Cytoplasmic Foci 0.0725 0.0476 0.057 0.0265 0.0531 0.0289 0.0327 0.0321 0.0325 0.0294 0.0411 0.0208 0.0245 0.0272 0.0311 0.0145 0.0108 0.0222
Eisosomes 0.0002 0 0.0002 0.0001 0.0003 0.0006 0.0009 0.0001 0.0003 0.0001 0 0.0001 0.0006 0.0003 0.0002 0.0003 0.0006 0.0001
Endoplasmic Reticulum 0.0024 0.0006 0.001 0.001 0.0009 0.0025 0.0014 0.0002 0.0027 0.0005 0.0009 0.0004 0.0051 0.0005 0.0016 0.0019 0.0027 0.0039
Endosome 0.0352 0.0225 0.0276 0.0134 0.0575 0.0177 0.0241 0.014 0.0143 0.0346 0.0339 0.0198 0.0424 0.0174 0.0156 0.0169 0.0324 0.0169
Golgi 0.0773 0.0884 0.1611 0.0817 0.2908 0.1409 0.1024 0.0632 0.1658 0.1997 0.1554 0.2053 0.1026 0.0692 0.0882 0.1183 0.0932 0.1246
Lipid Particles 0.6902 0.7947 0.6865 0.8231 0.4726 0.7689 0.77 0.8668 0.7335 0.7145 0.7548 0.725 0.6478 0.755 0.752 0.8173 0.4834 0.7591
Mitochondria 0.02 0.0012 0.011 0.0065 0.0381 0.0021 0.0174 0.0005 0.0043 0.0056 0.0023 0.0123 0.019 0.0078 0.0232 0.0132 0.042 0.0019
None 0.001 0 0.0007 0.0009 0.0002 0.0001 0.0004 0 0.001 0.0001 0 0.0002 0.0274 0.0001 0.0015 0 0.0018 0.0001
Nuclear Periphery 0.0052 0.0035 0.0037 0.0073 0.0009 0.0021 0.0052 0.0011 0.0026 0.0005 0.0008 0.0051 0.0202 0.0036 0.006 0.0027 0.0271 0.0146
Nucleolus 0.0254 0.0119 0.0175 0.0109 0.0009 0.0084 0.0106 0.0016 0.0057 0.0001 0.0002 0.0003 0.012 0.0614 0.0072 0.0002 0.002 0.0035
Nucleus 0.0118 0.0006 0.0025 0.007 0.0008 0.0011 0.0009 0.0003 0.0007 0 0 0.0001 0.005 0.0063 0.0011 0.0001 0.0981 0.0006
Peroxisomes 0.0067 0.006 0.0067 0.0033 0.024 0.0015 0.004 0.0011 0.0049 0.0025 0.0015 0.001 0.005 0.0039 0.0045 0.0026 0.0058 0.0013
Punctate Nuclear 0.0077 0.0007 0.0019 0.0016 0.0004 0.0005 0.001 0.0034 0.0022 0.0002 0.0002 0.0003 0.0029 0.006 0.0011 0.0005 0.0027 0.0312
Vacuole 0.0086 0.0034 0.002 0.0032 0.0094 0.0024 0.0027 0.0032 0.0023 0.001 0.0012 0.0014 0.0143 0.0073 0.0023 0.0007 0.0065 0.0006
Vacuole Periphery 0.0164 0.0084 0.0047 0.0099 0.0045 0.0044 0.0047 0.0059 0.0055 0.0042 0.0034 0.0046 0.0147 0.0064 0.0086 0.0041 0.0166 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 105.5775 76.6393 86.7087 77.8548 111.8416 87.2714 44.2458 36.4812 39.6867 76.0783
Translational Efficiency 1.3012 1.2672 1.1529 1.4697 1.2567 1.1831 1.0694 1.1719 1.0664 1.1142

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Faa4

Faa4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Faa4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available