Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 – 0.05 0 0.07 0 0 0 0 0 0.09 0.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Cytoplasm 0.73 0.77 0.88 0.64 0.61 0.46 – 0.4 0.31 0.32 0.28 0.28 0.84 0.91 0.96 0.23 0.38 0.25 0.3 0.15 0.36 0.33 0.28 0.34
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.1 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0.16 0.16 0.15 0.06 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Mitochondria 0.27 0.2 0.21 0.52 0.36 0.68 – 0.64 0.81 0.78 0.83 0.8 0.1 0 0 0.12 0.08 0.13 0.47 0.59 0.41 0.45 0.45 0.35
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.05 0 – 0.09 0 0.06 0 0.05 0 0 0 0.15 0.18 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.17 0.1 0.15 0.05 0.08 0 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 0 0 0 0 – 0 0 0 0 0 2 0 0 4 3 3 9 3 3 4 9 16
Bud 2 0 1 7 10 15 – 22 11 17 11 23 9 3 2 7 9 4 1 1 0 2 8 13
Bud Neck 3 0 3 0 3 0 – 8 0 3 0 1 0 1 0 0 1 2 2 1 0 3 7 10
Bud Site 0 0 1 0 4 1 – 12 2 5 2 14 0 0 0 0 2 1 – – – – – –
Cell Periphery 2 1 2 1 1 1 – 0 1 0 1 2 0 1 2 4 3 5 1 1 3 1 1 2
Cytoplasm 233 75 115 147 194 144 – 188 83 80 92 147 313 583 659 19 35 25 126 20 50 59 117 160
Endoplasmic Reticulum 9 1 0 0 2 0 – 1 1 1 2 0 3 2 0 7 9 8 2 1 2 2 3 1
Endosome 0 2 0 0 5 0 – 1 0 0 0 0 19 20 8 13 15 15 24 6 2 4 22 25
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 6 0 6 1 1 0 2 2 4
Mitochondria 88 20 28 119 113 212 – 302 217 193 277 422 36 25 13 10 7 13 200 82 56 80 189 163
Nucleus 1 1 1 0 0 2 – 6 2 3 2 1 0 3 1 2 5 3 0 0 1 0 1 1
Nuclear Periphery 1 0 0 0 1 0 – 2 4 0 2 2 0 0 0 0 3 0 0 0 0 0 2 0
Nucleolus 0 0 0 1 0 2 – 2 0 2 3 1 0 1 0 1 2 0 1 0 0 0 1 0
Peroxisomes 0 0 0 0 8 2 – 2 0 3 0 3 10 2 7 3 0 6 1 1 0 2 2 1
SpindlePole 2 3 1 4 17 8 – 43 5 15 14 28 8 4 1 12 16 9 14 3 4 3 16 13
Vac/Vac Membrane 8 1 0 1 7 6 – 9 6 3 1 5 7 9 10 14 9 15 23 10 5 6 30 40
Unique Cell Count 321 98 131 231 316 310 469 268 249 332 526 373 638 684 82 91 99 426 141 139 179 424 470
Labelled Cell Count 352 104 152 280 365 393 598 332 325 407 649 407 654 703 102 119 115 426 141 139 179 424 470


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 2.8 3.0 3.7 2.9 – 2.8 2.6 2.8 2.6 2.5 4.1 4.3 4.2 6.3 5.4 6.2 4.1 4.6 4.4
Std Deviation (1e-4) 0.5 0.7 0.8 1.4 1.7 1.1 – 1.0 1.4 1.3 1.2 0.9 1.1 1.3 1.3 1.5 1.2 1.6 1.5 1.9 1.5
Intensity Change (Log2) – – – 0.09 0.41 0.02 – 0.02 -0.09 -0.02 -0.11 -0.16 0.56 0.63 0.59 1.16 0.95 1.15 0.53 0.7 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 4.9 – 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.143 -0.0268 -0.1081 -0.1968 -0.3537 -0.5392 -0.3095 -0.7198 -0.7992 -0.7282 -0.1604 -0.8827 0.8514 0.5637 0.1172 1.0162 0.4418 0.6682
Actin 0.0217 0.0048 0.0084 0.0171 0.0039 0.0041 0.0353 0.0015 0.0229 0.0158 0.0151 0.006 0.0506 0.0023 0.0058 0.0562 0.0203 0.026
Bud 0.0016 0.0054 0.0036 0.0017 0.0016 0.0005 0.0066 0.0072 0.0044 0.0103 0.002 0.0011 0.0006 0.0004 0.0076 0.0013 0.0052 0.0009
Bud Neck 0.0019 0.0005 0.0032 0.0011 0.0135 0.0035 0.0148 0.001 0.0054 0.0013 0.0031 0.0099 0.0013 0.0003 0.0012 0.0013 0.0014 0.0114
Bud Periphery 0.0039 0.0026 0.0072 0.0025 0.0037 0.0015 0.0067 0.0058 0.0064 0.0137 0.0039 0.0033 0.0016 0.0008 0.0129 0.0017 0.0203 0.0016
Bud Site 0.0031 0.0125 0.0126 0.0049 0.0089 0.0006 0.0433 0.0338 0.0151 0.0268 0.0029 0.001 0.0044 0.0071 0.0067 0.0066 0.0068 0.001
Cell Periphery 0.0006 0.0008 0.0008 0.0004 0.0014 0.0002 0.0031 0.0016 0.0009 0.0006 0.0008 0.0004 0.0008 0.0009 0.0012 0.0002 0.0051 0.0006
Cytoplasm 0.0644 0.1251 0.1326 0.1248 0.1466 0.1801 0.0981 0.1482 0.1411 0.1325 0.1415 0.1826 0.0465 0.1382 0.102 0.071 0.0887 0.1654
Cytoplasmic Foci 0.1318 0.0691 0.0888 0.0714 0.08 0.1255 0.105 0.0857 0.1114 0.0527 0.1307 0.126 0.0758 0.0536 0.0805 0.0577 0.0847 0.0701
Eisosomes 0.0009 0.0005 0.0006 0.0004 0.0002 0.0002 0.0008 0.0007 0.0011 0.0002 0.0006 0.0002 0.0008 0.0007 0.0007 0.0005 0.0018 0.0006
Endoplasmic Reticulum 0.0049 0.0023 0.0073 0.0047 0.0068 0.0044 0.0075 0.0022 0.0039 0.0032 0.0026 0.0025 0.0104 0.0042 0.0029 0.0025 0.0069 0.0029
Endosome 0.0767 0.0496 0.0739 0.1019 0.0953 0.0977 0.0738 0.0246 0.0672 0.0615 0.1059 0.0672 0.0541 0.0171 0.0509 0.0791 0.1129 0.0627
Golgi 0.0243 0.0118 0.0238 0.0278 0.0165 0.0152 0.0161 0.0071 0.0127 0.0113 0.0397 0.0134 0.0118 0.0065 0.0112 0.034 0.0122 0.0246
Lipid Particles 0.0463 0.0045 0.0066 0.0097 0.0366 0.0246 0.039 0.0034 0.0104 0.0088 0.0463 0.0136 0.0246 0.0044 0.0116 0.018 0.0608 0.0154
Mitochondria 0.0392 0.0359 0.0772 0.0886 0.1345 0.0537 0.0239 0.0353 0.0438 0.0491 0.0657 0.0535 0.0151 0.0151 0.0583 0.0255 0.0681 0.0517
None 0.486 0.6541 0.5023 0.5063 0.3854 0.4444 0.4356 0.6153 0.503 0.5709 0.3223 0.4804 0.6548 0.7322 0.6225 0.6162 0.4276 0.5302
Nuclear Periphery 0.0202 0.0007 0.008 0.0046 0.0019 0.0053 0.0111 0.0008 0.0044 0.0025 0.0076 0.0013 0.0067 0.0008 0.0013 0.0021 0.0061 0.0022
Nucleolus 0.0023 0.0014 0.0008 0.0005 0.001 0.0008 0.0041 0.0014 0.0021 0.002 0.0017 0.0007 0.0012 0.0003 0.0012 0.0003 0.0014 0.0005
Nucleus 0.0068 0.0031 0.004 0.0021 0.0022 0.0034 0.0079 0.0028 0.006 0.0048 0.0027 0.0018 0.0015 0.0008 0.0017 0.0009 0.0028 0.0012
Peroxisomes 0.0441 0.0059 0.0213 0.0129 0.0299 0.0227 0.03 0.0117 0.0167 0.0184 0.0692 0.0233 0.0215 0.0109 0.0099 0.0166 0.0195 0.0191
Punctate Nuclear 0.0084 0.0021 0.0031 0.0025 0.001 0.0028 0.0213 0.0033 0.0036 0.004 0.0052 0.0053 0.0058 0.0006 0.0023 0.0026 0.041 0.0022
Vacuole 0.0081 0.0058 0.0095 0.0088 0.0239 0.006 0.0131 0.0056 0.0147 0.007 0.0213 0.0045 0.0079 0.0022 0.006 0.0033 0.0045 0.0068
Vacuole Periphery 0.003 0.0014 0.0044 0.005 0.0051 0.0028 0.003 0.0011 0.0029 0.0027 0.0094 0.0019 0.0022 0.0005 0.0015 0.0022 0.002 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.6068 9.742 14.4579 21.7844 22.9604 13.6147 27.0878 25.7616 27.7623 21.4372
Translational Efficiency 0.8168 1.1789 0.1998 0.1509 0.512 1.0869 0.3994 0.1651 0.1646 0.5439

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1625 1326 815 1333 990 1803 2697 1451 2615 3129 3512 2784

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.81 753.58 795.13 807.11 586.22 762.70 807.44 820.90 637.54 758.84 804.58 814.30
Standard Deviation 94.80 94.25 86.85 117.49 71.28 99.76 96.89 110.85 95.46 97.57 94.79 114.29
Intensity Change Log 2 — 0.172164 0.249594 0.271169 — 0.379674 0.461913 0.485764 — 0.272814 0.352666 0.375388

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000571 0.001410 0.000129 0.003636 0.000552 0.000861 0.001326 0.004588 0.000563 0.001094 0.001048 0.004132
Bud Neck 0.048209 0.061810 0.011836 0.028891 0.037804 0.062581 0.045482 0.033921 0.044270 0.062254 0.037674 0.031513
Bud Site 0.012635 0.036135 0.007235 0.046837 0.015749 0.017358 0.029897 0.067655 0.013814 0.025315 0.024638 0.057687
Cell Periphery 0.000443 0.000150 0.000312 0.000258 0.000929 0.000200 0.000152 0.000256 0.000627 0.000179 0.000189 0.000257
Cytoplasm 0.245135* 0.148534* 0.275108* 0.235853* 0.206926* 0.119638* 0.386677* 0.268284* 0.230670* 0.131884* 0.360786* 0.252756*
Cytoplasmic Foci 0.359524* 0.320977* 0.082231 0.130469 0.303441* 0.264904* 0.043429 0.127817 0.338292* 0.288667* 0.052433 0.129087
Eisosomes 0.000343 0.000220 0.000124 0.000163 0.001048 0.000242 0.000055 0.000157 0.000609 0.000233 0.000071 0.000160
Endoplasmic Reticulum 0.001119 0.000576 0.002024 0.003946 0.001460 0.001021 0.002316 0.002399 0.001248 0.000833 0.002248 0.003140
Endosome 0.046795 0.061589 0.017343 0.051371 0.022838 0.104071 0.012218 0.037989 0.037725 0.086068 0.013407 0.044396
Golgi 0.016901 0.041856 0.000867 0.012012 0.026212 0.032768 0.002274 0.009558 0.020426 0.036619 0.001948 0.010733
Lipid Particles 0.029080 0.006945 0.014402 0.008649 0.037923 0.024205 0.002931 0.005929 0.032428 0.016891 0.005593 0.007231
Mitochondria 0.059474 0.112064* 0.004119 0.027503 0.207272* 0.140453* 0.011749 0.019228 0.115428* 0.128422* 0.009978 0.023190
Mitotic Spindle 0.001882 0.020969 0.001418 0.049342 0.001793 0.014048 0.063961 0.048812 0.001848 0.016981 0.049447 0.049066
None 0.002906 0.000824 0.010640 0.001534 0.003510 0.001142 0.003199 0.002009 0.003135 0.001008 0.004926 0.001782
Nuclear Periphery 0.000527 0.000220 0.002499 0.002444 0.000415 0.000605 0.003225 0.001372 0.000485 0.000442 0.003056 0.001886
Nuclear Periphery Foci 0.000243 0.000483 0.000851 0.002063 0.000319 0.000312 0.000556 0.001302 0.000272 0.000385 0.000624 0.001666
Nucleolus 0.001762 0.001325 0.006471 0.003245 0.002718 0.002606 0.002659 0.002250 0.002124 0.002063 0.003544 0.002726
Nucleus 0.076856 0.030421 0.499598* 0.129978 0.061309 0.041240 0.285873* 0.130012* 0.070970 0.036655 0.335470* 0.129996
Peroxisomes 0.034835 0.080074 0.004928 0.034442 0.045082 0.047371 0.008829 0.039768 0.038714 0.061230 0.007924 0.037217
Vacuole 0.056733 0.067378 0.055817 0.216961* 0.016522 0.113701 0.091519 0.190216 0.041510 0.094070 0.083234 0.203022*
Vacuole Periphery 0.004027 0.006036 0.002049 0.010403 0.006179 0.010672 0.001672 0.006479 0.004842 0.008707 0.001760 0.008358

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.10 2.05 -4.13 -2.81 -5.15 -0.60 -1.67 -5.15 -3.60 -3.94 -1.60 -1.06 -6.48 -4.50 -5.41
Bud Neck -3.14 12.16 6.95 9.31 -6.08 -6.34 -1.55 2.28 9.74 4.83 -6.24 3.46 6.86 13.59 3.79
Bud Site -9.60 2.75 -8.24 -0.77 -9.36 -0.62 -2.85 -9.35 -9.69 -7.95 -7.09 -3.41 -12.86 -8.23 -10.23
Cell Periphery 2.90 1.28 2.19 -2.78 2.56 5.22 5.72 5.10 -0.69 -4.43 5.42 5.47 4.92 -2.30 -2.55
Cytoplasm 11.35 -2.47 1.39 -9.11 3.51 10.31 -15.98 -5.49 -17.66 10.45 16.77 -15.45 -2.47 -19.21 12.72
Cytoplasmic Foci 4.60 38.66 34.36 27.19 -5.44 4.39 36.41 23.29 23.46 -16.68 8.27 60.10 41.70 35.42 -19.51
Eisosomes 4.06 11.34 8.62 2.29 -2.04 5.43 6.70 6.07 5.28 -9.41 6.41 9.31 7.89 5.14 -9.88
Endoplasmic Reticulum 3.67 -3.26 -8.19 -10.46 -4.46 1.92 -6.07 -4.40 -8.11 1.04 3.15 -9.31 -9.55 -12.98 -2.80
Endosome -3.81 9.79 2.02 5.65 -7.66 -22.23 7.03 -2.72 19.66 -9.65 -17.24 14.79 1.35 18.78 -13.95
Golgi -9.58 13.52 5.36 13.15 -7.08 -2.43 13.53 9.60 12.67 -5.33 -9.10 18.55 10.54 18.13 -8.37
Lipid Particles 12.15 6.28 11.73 -1.21 4.50 4.96 15.30 13.99 11.36 -5.12 9.25 18.56 18.10 10.19 -1.80
Mitochondria -7.77 14.61 9.09 15.39 -6.56 6.75 22.39 21.47 23.64 -2.61 -2.01 24.83 21.79 27.85 -5.82
Mitotic Spindle -8.42 0.31 -10.31 -4.57 -10.33 -6.42 -15.88 -10.84 -6.70 2.56 -10.43 -15.75 -14.98 -8.11 -0.03
None 3.68 -3.49 2.47 -2.32 4.32 3.35 0.63 2.45 -1.41 3.83 4.69 -2.50 3.31 -2.21 6.05
Nuclear Periphery 3.64 -4.10 -8.19 -9.46 -0.83 -1.92 -21.18 -7.11 -6.17 12.03 0.48 -19.08 -10.57 -11.08 6.92
Nuclear Periphery Foci -0.82 -5.02 -9.33 -4.16 -4.45 0.11 -3.65 -4.29 -4.56 -2.43 -0.86 -6.67 -9.51 -6.32 -5.52
Nucleolus 1.34 -5.76 -3.38 -4.42 3.70 0.05 -0.71 0.58 0.77 2.27 -0.02 -4.97 -1.79 -1.96 3.39
Nucleus 12.48 -37.58 -10.89 -19.34 29.35 4.28 -38.96 -12.96 -18.18 23.44 11.71 -52.74 -16.70 -26.26 34.88
Peroxisomes -11.09 14.34 2.19 12.02 -9.07 -0.66 13.53 3.48 4.47 -9.80 -8.71 19.06 3.59 11.56 -12.96
Vacuole -3.25 -0.16 -28.29 -25.38 -27.18 -22.39 -29.53 -33.93 -16.89 -17.05 -16.73 -20.85 -42.13 -28.90 -27.45
Vacuole Periphery -2.73 4.80 -3.18 -1.19 -5.14 -6.54 10.01 1.66 6.39 -4.19 -8.12 11.90 -1.58 3.62 -7.00
Standard name
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YMR252C is not an essential gene
Localization
Cell Percentages mitochondrion (32%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mlo1

Mlo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available