ORF | |
---|---|
Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 11 | 9 | 2 | 2 | 3 | 8 | 4 | 4 | 7 | 1 | 0 | 27 | 13 | 7 | 23 | 11 | 15 | 6 | 8 | 8 | 3 | 6 | 6 |
Bud | 5 | 8 | 5 | 1 | 8 | 21 | 23 | 32 | 17 | 32 | 18 | 21 | 3 | 2 | 2 | 5 | 9 | 5 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | 1 | 2 | 5 | 7 | 6 | 9 | 4 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 0 | 0 | 1 | 6 | 2 | 0 | 3 | 3 | 1 | 1 | 4 | 2 | 2 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 6 | 9 | 8 | 12 | 7 | 23 | 12 | 6 | 11 | 7 | 10 | 16 | 17 | 18 | 17 | 14 | 20 | 0 | 0 | 1 | 3 | 2 | 2 |
Endoplasmic Reticulum | 3 | 13 | 3 | 2 | 1 | 2 | 7 | 1 | 1 | 3 | 1 | 1 | 8 | 1 | 2 | 8 | 11 | 13 | 0 | 0 | 2 | 1 | 3 | 3 |
Endosome | 16 | 14 | 29 | 23 | 74 | 25 | 19 | 82 | 7 | 7 | 5 | 1 | 50 | 55 | 68 | 108 | 86 | 90 | 63 | 59 | 37 | 93 | 129 | 79 |
Golgi | 25 | 71 | 67 | 15 | 10 | 7 | 10 | 3 | 8 | 4 | 2 | 1 | 273 | 338 | 243 | 137 | 120 | 134 | 146 | 149 | 124 | 156 | 243 | 283 |
Mitochondria | 225 | 212 | 126 | 76 | 93 | 191 | 250 | 257 | 204 | 232 | 183 | 137 | 164 | 113 | 156 | 23 | 24 | 26 | 12 | 31 | 6 | 21 | 35 | 28 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 3 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 1 | 3 | 1 | 1 | 1 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 22 | 7 | 5 | 5 | 4 | 13 | 16 | 17 | 5 | 10 | 9 | 11 | 5 | 1 | 9 | 19 | 12 | 16 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 9 | 2 | 21 | 12 | 4 | 36 | 6 | 5 | 4 | 3 | 8 | 9 | 16 | 8 | 8 | 4 | 26 | 7 | 21 | 10 | 7 | 10 |
SpindlePole | 9 | 0 | 4 | 0 | 12 | 8 | 16 | 29 | 5 | 11 | 8 | 4 | 7 | 9 | 15 | 37 | 26 | 23 | 2 | 4 | 2 | 1 | 4 | 2 |
Vac/Vac Membrane | 14 | 23 | 3 | 5 | 11 | 15 | 17 | 12 | 12 | 8 | 8 | 1 | 26 | 54 | 41 | 53 | 56 | 68 | 1 | 5 | 2 | 11 | 15 | 9 |
Unique Cell Count | 249 | 245 | 190 | 106 | 179 | 240 | 322 | 379 | 233 | 264 | 203 | 158 | 396 | 475 | 439 | 325 | 285 | 318 | 261 | 270 | 210 | 308 | 455 | 431 |
Labelled Cell Count | 331 | 368 | 272 | 139 | 251 | 307 | 406 | 495 | 282 | 346 | 255 | 195 | 591 | 620 | 582 | 444 | 389 | 418 | 261 | 270 | 210 | 308 | 455 | 431 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.0 | 8.7 | 7.4 | 7.4 | 6.8 | 6.9 | 6.3 | 6.1 | 6.1 | 5.7 | 6.0 | 10.8 | 11.0 | 10.4 | 10.3 | 9.9 | 9.8 | 9.2 | 9.5 | 10.0 |
Std Deviation (1e-4) | 1.2 | 1.6 | 2.4 | 1.9 | 1.4 | 2.1 | 3.0 | 1.2 | 1.2 | 1.2 | 1.1 | 1.2 | 1.9 | 2.0 | 2.1 | 2.2 | 2.1 | 2.1 | 1.7 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.24 | -0.36 | -0.33 | -0.48 | -0.51 | -0.53 | -0.62 | -0.54 | 0.3 | 0.34 | 0.24 | 0.24 | 0.18 | 0.17 | 0.08 | 0.12 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -2.0 | -2.2 | -1.4 | 0 | -1.8 | -1.2 | 0 | 0 | 1.0 | -1.3 | 0 | 1.1 | -0.5 | 0 |
Bud | 0 | 1.0 | 2.6 | 2.2 | 2.7 | 2.1 | 3.6 | 2.6 | 3.8 | 0 | 0 | 0 | 0 | 0.3 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 0.8 | -1.0 | 1.1 | -0.9 | -1.2 | -0.3 | -0.6 | 0.7 | -0.4 | -0.7 | -0.4 | 0.2 | 0.1 | 0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 1.6 |
Endosome | 1.4 | 5.6 | -1.5 | -3.5 | 1.8 | -4.5 | -4.9 | -4.5 | -4.8 | -0.9 | -1.3 | 0.1 | 4.5 | 3.7 | 3.4 |
Golgi | -3.9 | -7.0 | -8.8 | -9.8 | -11.8 | -8.5 | -9.8 | -8.9 | -8.1 | 7.7 | 8.6 | 4.6 | 1.5 | 1.5 | 1.5 |
Mitochondria | 1.0 | -2.8 | 3.1 | 2.8 | 0.4 | 5.2 | 5.6 | 5.8 | 4.4 | -5.6 | -10.3 | -7.1 | -14.3 | -13.3 | -13.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1.4 | 1.3 | 1.1 | 0 | 0.7 | 1.0 | 1.9 | 0 | 0 | 0 | 1.7 | 0.9 | 1.3 |
Peroxisomes | 0 | 2.5 | 0.1 | 0 | 2.0 | -1.2 | -1.7 | -1.5 | -1.4 | -1.8 | -2.0 | -0.6 | -1.4 | -1.1 | 0 |
SpindlePole | 0 | 2.2 | 0.8 | 1.6 | 2.7 | 0 | 1.2 | 1.1 | 0 | 0 | 0 | 0.9 | 3.8 | 3.1 | 2.5 |
Vacuole | 0 | 2.3 | 2.4 | 2.1 | 1.1 | 2.0 | 0 | 1.4 | 0 | 2.6 | 4.1 | 3.5 | 5.2 | 5.8 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.6839 | 5.5275 | 4.8746 | 4.3241 | 4.2642 | 5.0454 | 2.8051 | 3.582 | 3.0758 | 2.5809 | 3.4953 | 3.7933 | 3.1172 | 4.144 | 3.6248 | 3.4186 | 3.0742 | 3.8778 |
Actin | 0.0244 | 0.0087 | 0.0287 | 0.0204 | 0.0206 | 0.0113 | 0.0283 | 0.0029 | 0.0103 | 0.0003 | 0.0001 | 0.0056 | 0.0304 | 0.0023 | 0.0026 | 0.0167 | 0.0414 | 0.011 |
Bud | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.001 | 0.0003 | 0.0005 | 0 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0.0006 | 0.0002 |
Bud Neck | 0.0004 | 0.0012 | 0.0005 | 0.0018 | 0.0003 | 0.0004 | 0.0011 | 0.0004 | 0.0006 | 0.0002 | 0.0001 | 0.0006 | 0.0009 | 0.0003 | 0.0002 | 0.0003 | 0.0005 | 0.0007 |
Bud Periphery | 0.0007 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0013 | 0.0002 | 0.0007 | 0 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0005 | 0.0015 | 0.0003 |
Bud Site | 0.0011 | 0.0037 | 0.0011 | 0.0008 | 0.0004 | 0.0002 | 0.0067 | 0.0041 | 0.0025 | 0.0001 | 0 | 0.0002 | 0.0015 | 0.0006 | 0.0003 | 0.0013 | 0.0017 | 0.0004 |
Cell Periphery | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 |
Cytoplasm | 0.0113 | 0.0027 | 0.0028 | 0.0018 | 0.0021 | 0.0012 | 0.0027 | 0.0084 | 0.0064 | 0.0009 | 0.0009 | 0.0034 | 0.0065 | 0.0027 | 0.0018 | 0.001 | 0.0007 | 0.0011 |
Cytoplasmic Foci | 0.129 | 0.1851 | 0.1541 | 0.1495 | 0.1458 | 0.1264 | 0.1125 | 0.1913 | 0.1903 | 0.1858 | 0.1233 | 0.1068 | 0.1523 | 0.1322 | 0.1258 | 0.0969 | 0.0992 | 0.1008 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0007 | 0.0002 | 0 | 0.0003 | 0 | 0.0029 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0042 | 0.0003 | 0.0005 | 0.0005 | 0.0013 | 0.0007 | 0.0027 | 0.0006 | 0.0016 | 0.0001 | 0.0003 | 0.0007 | 0.0021 | 0.0004 | 0.0009 | 0.0002 | 0.0008 | 0.0015 |
Endosome | 0.278 | 0.2868 | 0.2024 | 0.3175 | 0.2521 | 0.1898 | 0.4293 | 0.4212 | 0.382 | 0.3479 | 0.4544 | 0.2621 | 0.2884 | 0.3276 | 0.2913 | 0.3363 | 0.1941 | 0.1937 |
Golgi | 0.3666 | 0.4462 | 0.4153 | 0.3662 | 0.4801 | 0.5395 | 0.1761 | 0.3016 | 0.306 | 0.3904 | 0.3998 | 0.504 | 0.3452 | 0.4468 | 0.469 | 0.3943 | 0.471 | 0.6009 |
Lipid Particles | 0.0532 | 0.0144 | 0.032 | 0.035 | 0.0244 | 0.0266 | 0.1471 | 0.0215 | 0.0308 | 0.0349 | 0.0084 | 0.0545 | 0.078 | 0.0349 | 0.0295 | 0.016 | 0.043 | 0.0275 |
Mitochondria | 0.0242 | 0.0154 | 0.0442 | 0.0372 | 0.0151 | 0.025 | 0.0274 | 0.0144 | 0.0241 | 0.0038 | 0.0059 | 0.0231 | 0.0132 | 0.0246 | 0.033 | 0.0713 | 0.0964 | 0.0221 |
None | 0.0075 | 0.0002 | 0.0003 | 0.001 | 0.0005 | 0.0002 | 0.0009 | 0.0002 | 0.0004 | 0 | 0 | 0.0001 | 0.0084 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0002 |
Nuclear Periphery | 0.0151 | 0.0001 | 0.0058 | 0.0002 | 0.0006 | 0.0027 | 0.0109 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0024 | 0.0002 | 0.0002 | 0.0002 | 0.0014 | 0.0042 |
Nucleolus | 0.0003 | 0.0002 | 0.0018 | 0.0002 | 0 | 0 | 0.0007 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 |
Nucleus | 0.0023 | 0.0002 | 0.0081 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.0002 |
Peroxisomes | 0.0677 | 0.0286 | 0.0915 | 0.0606 | 0.0521 | 0.0728 | 0.023 | 0.0243 | 0.034 | 0.0329 | 0.0022 | 0.0336 | 0.0525 | 0.0182 | 0.0404 | 0.0601 | 0.0253 | 0.0294 |
Punctate Nuclear | 0.0034 | 0.0001 | 0.0032 | 0.0014 | 0.0017 | 0.0004 | 0.008 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0.0003 | 0.0084 | 0.0002 | 0.0004 | 0.0006 | 0.0004 | 0.0006 |
Vacuole | 0.0057 | 0.0029 | 0.0051 | 0.0021 | 0.0009 | 0.001 | 0.0115 | 0.0046 | 0.0026 | 0.0011 | 0.002 | 0.0018 | 0.0046 | 0.0055 | 0.0018 | 0.0014 | 0.0073 | 0.002 |
Vacuole Periphery | 0.004 | 0.0019 | 0.002 | 0.0027 | 0.0012 | 0.0014 | 0.0066 | 0.0029 | 0.0021 | 0.0011 | 0.0024 | 0.0025 | 0.003 | 0.0029 | 0.0021 | 0.0016 | 0.0117 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.9882 | 7.1304 | 22.928 | 23.4491 | 8.0436 | 3.5431 | 20.6274 | 21.0859 | 27.6578 | 15.9732 |
Translational Efficiency | 1.6692 | 1.73 | 0.8842 | 1.0441 | 0.8333 | 2.7966 | 0.9594 | 0.9909 | 0.9799 | 1.0375 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
242 | 131 | 1879 | 1532 | 1809 | 1689 | 2264 | 1049 | 2051 | 1820 | 4143 | 2581 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 985.86 | 921.17 | 1061.37 | 1029.79 | 1018.95 | 930.41 | 1125.66 | 1031.48 | 1015.05 | 929.74 | 1096.50 | 1030.48 |
Standard Deviation | 127.98 | 133.09 | 159.92 | 160.91 | 159.04 | 123.11 | 150.38 | 171.31 | 156.06 | 123.87 | 158.05 | 165.22 |
Intensity Change Log 2 | — | -0.097915 | 0.106473 | 0.062895 | — | -0.131145 | 0.143688 | 0.017633 | — | -0.114709 | 0.125507 | 0.040068 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.006537 | 0.012167 | 0.011243 | 0.019992 | 0.005413 | 0.003230 | 0.006768 | 0.007576 | 0.005546 | 0.003874 | 0.008797 | 0.014946 |
Bud Neck | 0.000275 | 0.000797 | 0.000660 | 0.001008 | 0.000347 | 0.000187 | 0.000228 | 0.002266 | 0.000339 | 0.000231 | 0.000424 | 0.001519 |
Bud Site | 0.001010 | 0.015019 | 0.001737 | 0.017502 | 0.000976 | 0.002336 | 0.002637 | 0.012461 | 0.000980 | 0.003249 | 0.002229 | 0.015453 |
Cell Periphery | 0.000057 | 0.000068 | 0.000060 | 0.000154 | 0.000090 | 0.000054 | 0.000052 | 0.000372 | 0.000086 | 0.000055 | 0.000056 | 0.000242 |
Cytoplasm | 0.000525 | 0.007137 | 0.000739 | 0.007393 | 0.000581 | 0.001992 | 0.001200 | 0.007259 | 0.000574 | 0.002362 | 0.000991 | 0.007338 |
Cytoplasmic Foci | 0.232567* | 0.295119* | 0.275870* | 0.193157* | 0.220147* | 0.235745* | 0.218064* | 0.253446* | 0.221612* | 0.240018* | 0.244281* | 0.217660* |
Eisosomes | 0.000054 | 0.000080 | 0.000066 | 0.000082 | 0.000088 | 0.000041 | 0.000049 | 0.000050 | 0.000084 | 0.000044 | 0.000057 | 0.000069 |
Endoplasmic Reticulum | 0.000280 | 0.000234 | 0.000550 | 0.000613 | 0.000455 | 0.000173 | 0.000263 | 0.000543 | 0.000434 | 0.000177 | 0.000393 | 0.000585 |
Endosome | 0.210436* | 0.274381* | 0.253876* | 0.236055* | 0.142099 | 0.264163* | 0.221896* | 0.332128* | 0.150162 | 0.264898* | 0.236400* | 0.275102* |
Golgi | 0.506662* | 0.313003* | 0.341751* | 0.428021* | 0.483424* | 0.426970* | 0.497612* | 0.253202* | 0.486166* | 0.418767* | 0.426923* | 0.356969* |
Lipid Particles | 0.004371 | 0.005686 | 0.047560 | 0.004898 | 0.018845 | 0.004778 | 0.004419 | 0.008435 | 0.017138 | 0.004843 | 0.023985 | 0.006336 |
Mitochondria | 0.014917 | 0.008030 | 0.010218 | 0.017247 | 0.101987* | 0.028081 | 0.016229 | 0.010929 | 0.091713 | 0.026637 | 0.013503 | 0.014679 |
Mitotic Spindle | 0.001214 | 0.013928 | 0.021988 | 0.021777 | 0.002473 | 0.005118 | 0.005332 | 0.041956 | 0.002324 | 0.005752 | 0.012886 | 0.029978 |
None | 0.000014 | 0.000031 | 0.000026 | 0.000467 | 0.000084 | 0.000021 | 0.000021 | 0.000052 | 0.000076 | 0.000022 | 0.000023 | 0.000298 |
Nuclear Periphery | 0.000020 | 0.000020 | 0.000537 | 0.000168 | 0.000177 | 0.000055 | 0.000155 | 0.000443 | 0.000159 | 0.000053 | 0.000328 | 0.000280 |
Nuclear Periphery Foci | 0.000090 | 0.000225 | 0.003596 | 0.000787 | 0.000301 | 0.000374 | 0.000519 | 0.002610 | 0.000276 | 0.000363 | 0.001915 | 0.001528 |
Nucleolus | 0.000004 | 0.000039 | 0.000067 | 0.000065 | 0.000015 | 0.000015 | 0.000007 | 0.000372 | 0.000013 | 0.000017 | 0.000034 | 0.000190 |
Nucleus | 0.000007 | 0.000058 | 0.000058 | 0.000079 | 0.000021 | 0.000027 | 0.000010 | 0.000481 | 0.000019 | 0.000029 | 0.000032 | 0.000243 |
Peroxisomes | 0.014793 | 0.045662 | 0.008754 | 0.012196 | 0.005399 | 0.010053 | 0.003468 | 0.021635 | 0.006508 | 0.012616 | 0.005865 | 0.016032 |
Vacuole | 0.002967 | 0.005832 | 0.014407 | 0.028388 | 0.006988 | 0.009410 | 0.013213 | 0.033802 | 0.006514 | 0.009153 | 0.013755 | 0.030588 |
Vacuole Periphery | 0.003199 | 0.002481 | 0.006238 | 0.009951 | 0.010091 | 0.007178 | 0.007858 | 0.009983 | 0.009278 | 0.006840 | 0.007123 | 0.009964 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.15 | -1.58 | -4.00 | -1.66 | -3.30 | 2.23 | -1.13 | -1.29 | -2.64 | -0.45 | 1.70 | -2.91 | -5.81 | -6.86 | -3.66 |
Bud Neck | -2.14 | -3.37 | -5.34 | -1.02 | -2.98 | 2.45 | 1.18 | -5.21 | -5.70 | -5.48 | 1.75 | -1.53 | -7.03 | -8.01 | -6.60 |
Bud Site | -1.88 | -2.94 | -10.63 | -0.45 | -10.22 | -2.71 | -4.15 | -8.50 | -7.27 | -7.15 | -3.19 | -4.62 | -13.42 | -9.91 | -12.25 |
Cell Periphery | -0.68 | -0.28 | -4.72 | -3.90 | -5.26 | 5.73 | 7.21 | -0.95 | -1.07 | -1.08 | 5.24 | 6.99 | -1.30 | -1.56 | -1.55 |
Cytoplasm | -2.04 | -2.26 | -6.77 | -0.30 | -6.58 | -2.95 | -2.22 | -5.70 | -4.45 | -5.14 | -3.57 | -2.68 | -8.83 | -5.97 | -8.23 |
Cytoplasmic Foci | -3.53 | -4.15 | 3.92 | 6.57 | 13.62 | -2.78 | 0.40 | -4.69 | -2.39 | -5.15 | -3.45 | -4.95 | 0.96 | 4.43 | 6.15 |
Eisosomes | -1.47 | -1.24 | -2.73 | -0.13 | -1.81 | 9.40 | 8.16 | 3.30 | -0.81 | -0.10 | 8.49 | 5.75 | 2.12 | -3.81 | -1.87 |
Endoplasmic Reticulum | 0.28 | -1.88 | -1.98 | -3.06 | -0.60 | 6.64 | 4.42 | -0.64 | -2.75 | -2.07 | 6.13 | 0.88 | -1.78 | -5.11 | -2.36 |
Endosome | -2.94 | -3.48 | -2.01 | 2.04 | 2.63 | -20.55 | -15.92 | -23.30 | -7.56 | -13.28 | -19.92 | -18.93 | -22.95 | -1.42 | -7.18 |
Golgi | 8.04 | 10.34 | 5.01 | -5.50 | -8.78 | 7.17 | -1.82 | 25.22 | 19.47 | 28.01* | 9.00 | 8.84 | 17.38 | 8.56 | 10.61 |
Lipid Particles | -0.63 | -13.12 | -0.41 | 0.39 | 13.44 | 8.02 | 8.40 | 4.84 | -2.48 | -2.79 | 7.82 | -3.35 | 6.63 | -1.64 | 10.76 |
Mitochondria | 1.21 | 1.07 | -0.49 | -2.22 | -3.32 | 13.79 | 16.82 | 17.57 | 6.07 | 2.33 | 13.43 | 17.47 | 16.90 | 4.91 | -0.69 |
Mitotic Spindle | -2.18 | -8.63 | -9.09 | -1.31 | 0.08 | -2.39 | -3.17 | -10.08 | -9.22 | -9.26 | -3.10 | -8.63 | -13.18 | -10.67 | -7.33 |
None | -0.90 | -3.84 | -1.29 | -1.24 | -1.26 | 12.63 | 12.25 | 1.11 | -1.65 | -1.68 | 11.48 | 12.09 | -1.08 | -1.34 | -1.33 |
Nuclear Periphery | 0.11 | -5.82 | -3.11 | -3.12 | 3.64 | 5.38 | 0.50 | -1.85 | -2.72 | -1.95 | 5.22 | -3.56 | -1.84 | -3.49 | 0.62 |
Nuclear Periphery Foci | -1.82 | -6.00 | -4.19 | -3.19 | 4.63 | -0.44 | -1.47 | -3.15 | -2.96 | -2.78 | -0.56 | -5.89 | -4.02 | -3.31 | 1.04 |
Nucleolus | -1.69 | -4.07 | -3.28 | -1.04 | 0.26 | -0.08 | 2.87 | -2.23 | -2.23 | -2.28 | -0.76 | -3.00 | -2.70 | -2.64 | -2.31 |
Nucleus | -1.30 | -4.23 | -2.43 | -1.18 | -1.34 | -0.60 | 3.32 | -2.07 | -2.04 | -2.11 | -1.04 | -2.35 | -2.67 | -2.56 | -2.51 |
Peroxisomes | -2.90 | 1.77 | 0.69 | 3.27 | -2.53 | -3.98 | 2.75 | -7.53 | -5.07 | -8.67 | -4.46 | 0.77 | -7.51 | -2.20 | -8.81 |
Vacuole | -2.43 | -10.02 | -13.30 | -10.76 | -6.99 | -2.65 | -6.41 | -11.70 | -10.46 | -8.73 | -3.15 | -9.09 | -16.16 | -13.84 | -11.08 |
Vacuole Periphery | 0.65 | -2.80 | -5.41 | -7.67 | -3.92 | 3.57 | 2.98 | 0.16 | -2.41 | -1.89 | 3.24 | 3.46 | -0.81 | -3.74 | -3.95 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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