Standard name | |
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Human Ortholog | |
Description | Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | – | 0 | 16 | 20 | 24 | 11 | 25 | 35 |
Bud | 1 | 6 | 4 | 6 | 3 | 12 | – | 20 | 24 | 30 | 23 | 19 | 0 | 0 | 1 | – | – | 0 | 1 | 5 | 3 | 3 | 8 | 16 |
Bud Neck | 1 | 0 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 6 | 8 | 5 | – | – | 3 | 3 | 4 | 4 | 7 | 16 | 15 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 2 | – | 8 | 5 | 10 | 6 | 7 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 2 | 6 | 1 | 1 | – | 1 | 0 | 3 | 0 | 2 | 3 | 3 | 1 | – | – | 1 | 2 | 3 | 3 | 1 | 4 | 12 |
Cytoplasm | 137 | 171 | 147 | 97 | 96 | 100 | – | 110 | 63 | 47 | 43 | 31 | 280 | 280 | 380 | – | – | 171 | 37 | 53 | 23 | 28 | 65 | 74 |
Endoplasmic Reticulum | 2 | 11 | 1 | 1 | 2 | 4 | – | 1 | 4 | 6 | 7 | 1 | 3 | 2 | 2 | – | – | 17 | 4 | 5 | 10 | 3 | 5 | 11 |
Endosome | 21 | 19 | 48 | 29 | 75 | 15 | – | 25 | 7 | 19 | 0 | 3 | 1 | 1 | 0 | – | – | 13 | 33 | 44 | 40 | 19 | 36 | 45 |
Golgi | 0 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 0 | 2 | 6 | 6 | 3 | 3 | 3 |
Mitochondria | 35 | 73 | 78 | 78 | 99 | 364 | – | 573 | 529 | 508 | 531 | 464 | 0 | 0 | 1 | – | – | 8 | 177 | 225 | 242 | 143 | 308 | 290 |
Nucleus | 1 | 0 | 2 | 0 | 2 | 0 | – | 8 | 6 | 9 | 4 | 4 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | – | 1 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | – | – | 1 | 0 | 0 | 1 | 0 | 0 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 3 | 1 |
Peroxisomes | 8 | 2 | 31 | 34 | 37 | 6 | – | 39 | 1 | 26 | 2 | 19 | 0 | 0 | 0 | – | – | 6 | 2 | 3 | 11 | 5 | 4 | 4 |
SpindlePole | 7 | 7 | 29 | 17 | 26 | 23 | – | 59 | 27 | 34 | 8 | 32 | 0 | 0 | 2 | – | – | 13 | 8 | 11 | 19 | 14 | 27 | 23 |
Vac/Vac Membrane | 16 | 13 | 17 | 4 | 22 | 24 | – | 21 | 14 | 10 | 13 | 11 | 1 | 1 | 2 | – | – | 5 | 13 | 14 | 4 | 14 | 27 | 32 |
Unique Cell Count | 212 | 289 | 303 | 219 | 296 | 461 | 715 | 578 | 572 | 554 | 506 | 290 | 288 | 389 | 206 | 311 | 406 | 413 | 263 | 554 | 586 | |||
Labelled Cell Count | 232 | 307 | 363 | 272 | 366 | 554 | 868 | 681 | 706 | 639 | 596 | 295 | 295 | 397 | 238 | 311 | 406 | 413 | 263 | 554 | 586 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 4.2 | 3.9 | 3.9 | 3.8 | 3.3 | – | 3.3 | 3.0 | 2.8 | 2.8 | 3.0 | 5.4 | 5.4 | 5.5 | 4.0 | 4.6 | 5.0 | 5.4 | 5.4 | 5.6 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.4 | 1.6 | 1.0 | 1.3 | – | 1.0 | 0.8 | 0.7 | 0.8 | 0.7 | 1.5 | 1.5 | 1.2 | 1.0 | 1.1 | 1.4 | 1.7 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.06 | -0.28 | – | -0.28 | -0.39 | -0.47 | -0.47 | -0.42 | 0.45 | 0.45 | 0.48 | 0.02 | 0.21 | 0.35 | 0.44 | 0.46 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | -1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1133 | 0.5711 | 0.7274 | 0.121 | -0.1371 | 0.0815 | 2.1204 | 2.3795 | 2.5323 | 2.2194 | 1.7677 | 2.1566 | 1.9583 | 2.2535 | 1.8766 | 2.4431 | 2.3217 | 2.0584 |
Actin | 0.0054 | 0.0141 | 0.0159 | 0.0338 | 0.0477 | 0.0037 | 0.0093 | 0.0034 | 0.0256 | 0.0004 | 0.0114 | 0.0073 | 0.0382 | 0.0109 | 0.027 | 0.0152 | 0.0227 | 0.0128 |
Bud | 0.0003 | 0.0011 | 0.002 | 0.0014 | 0.0007 | 0.0012 | 0.0008 | 0.0019 | 0.0095 | 0.0009 | 0.0111 | 0.0013 | 0.0047 | 0.0088 | 0.0035 | 0.0012 | 0.0022 | 0.0004 |
Bud Neck | 0.0004 | 0.0022 | 0.0037 | 0.0008 | 0.0008 | 0.0098 | 0.0009 | 0.001 | 0.0024 | 0.0002 | 0.0039 | 0.0028 | 0.0104 | 0.0013 | 0.0047 | 0.0155 | 0.0062 | 0.0081 |
Bud Periphery | 0.0005 | 0.0013 | 0.0029 | 0.0037 | 0.0019 | 0.0022 | 0.002 | 0.0035 | 0.0181 | 0.0025 | 0.0234 | 0.0054 | 0.0164 | 0.0114 | 0.0091 | 0.0062 | 0.0063 | 0.0011 |
Bud Site | 0.002 | 0.0311 | 0.0468 | 0.0031 | 0.0014 | 0.0036 | 0.0027 | 0.0145 | 0.0318 | 0.0002 | 0.003 | 0.0005 | 0.0111 | 0.0464 | 0.0219 | 0.0027 | 0.0059 | 0.0005 |
Cell Periphery | 0.0014 | 0.0014 | 0.0014 | 0.0014 | 0.001 | 0.0016 | 0.0016 | 0.0015 | 0.0015 | 0.0005 | 0.0042 | 0.0019 | 0.0021 | 0.003 | 0.0014 | 0.0023 | 0.0014 | 0.0006 |
Cytoplasm | 0.1854 | 0.2532 | 0.2213 | 0.0979 | 0.3861 | 0.2668 | 0.1033 | 0.1491 | 0.1561 | 0.1487 | 0.2414 | 0.2234 | 0.1023 | 0.2027 | 0.1879 | 0.1983 | 0.2137 | 0.2296 |
Cytoplasmic Foci | 0.1156 | 0.1008 | 0.1138 | 0.0816 | 0.06 | 0.0928 | 0.1277 | 0.0849 | 0.0905 | 0.0198 | 0.0805 | 0.1025 | 0.158 | 0.0732 | 0.0964 | 0.0421 | 0.1146 | 0.0945 |
Eisosomes | 0.0004 | 0.0009 | 0.0007 | 0.0009 | 0.0005 | 0.0007 | 0.0007 | 0.0007 | 0.0008 | 0.0004 | 0.0006 | 0.0006 | 0.0029 | 0.002 | 0.001 | 0.0009 | 0.0015 | 0.0004 |
Endoplasmic Reticulum | 0.0074 | 0.013 | 0.0117 | 0.0059 | 0.0079 | 0.0075 | 0.0056 | 0.006 | 0.0042 | 0.0062 | 0.0029 | 0.0048 | 0.0038 | 0.0066 | 0.0084 | 0.0186 | 0.0027 | 0.0034 |
Endosome | 0.1093 | 0.078 | 0.1604 | 0.1313 | 0.0722 | 0.0404 | 0.0648 | 0.047 | 0.0571 | 0.1066 | 0.0345 | 0.0408 | 0.0983 | 0.0559 | 0.0663 | 0.0773 | 0.0638 | 0.0647 |
Golgi | 0.0139 | 0.0285 | 0.0402 | 0.0245 | 0.0217 | 0.0059 | 0.0101 | 0.0092 | 0.0182 | 0.0077 | 0.011 | 0.0125 | 0.0184 | 0.012 | 0.0106 | 0.0094 | 0.0263 | 0.0143 |
Lipid Particles | 0.0369 | 0.0112 | 0.0253 | 0.0677 | 0.0036 | 0.0102 | 0.0455 | 0.0114 | 0.0106 | 0.001 | 0.0015 | 0.0097 | 0.0301 | 0.003 | 0.0124 | 0.0148 | 0.0141 | 0.0043 |
Mitochondria | 0.0156 | 0.0132 | 0.0646 | 0.0676 | 0.0399 | 0.0102 | 0.0103 | 0.0273 | 0.0323 | 0.0663 | 0.0182 | 0.0519 | 0.0578 | 0.0348 | 0.0408 | 0.0446 | 0.0599 | 0.0479 |
None | 0.4285 | 0.4312 | 0.2442 | 0.4425 | 0.3357 | 0.5265 | 0.5462 | 0.6121 | 0.5031 | 0.5983 | 0.5317 | 0.4902 | 0.3803 | 0.5036 | 0.4571 | 0.5104 | 0.4163 | 0.4897 |
Nuclear Periphery | 0.007 | 0.0013 | 0.0032 | 0.0021 | 0.0011 | 0.0011 | 0.0141 | 0.0032 | 0.0035 | 0.002 | 0.0019 | 0.0012 | 0.0021 | 0.0013 | 0.0026 | 0.0059 | 0.001 | 0.0005 |
Nucleolus | 0.001 | 0.0004 | 0.0005 | 0.0012 | 0.0003 | 0.0007 | 0.0015 | 0.0013 | 0.0019 | 0.0002 | 0.0012 | 0.0003 | 0.0048 | 0.0012 | 0.0019 | 0.001 | 0.0015 | 0.0003 |
Nucleus | 0.0059 | 0.0009 | 0.0012 | 0.0014 | 0.0012 | 0.0012 | 0.0055 | 0.0029 | 0.005 | 0.001 | 0.002 | 0.0026 | 0.004 | 0.0022 | 0.0033 | 0.0025 | 0.0014 | 0.0007 |
Peroxisomes | 0.0269 | 0.0099 | 0.0277 | 0.0133 | 0.0064 | 0.0076 | 0.0317 | 0.0054 | 0.0132 | 0.0007 | 0.0041 | 0.0321 | 0.032 | 0.0107 | 0.0248 | 0.0169 | 0.0203 | 0.0195 |
Punctate Nuclear | 0.0193 | 0.0012 | 0.0017 | 0.01 | 0.0004 | 0.001 | 0.0045 | 0.007 | 0.0032 | 0.0003 | 0.006 | 0.0008 | 0.0122 | 0.0011 | 0.0076 | 0.0025 | 0.0013 | 0.001 |
Vacuole | 0.0149 | 0.0038 | 0.0075 | 0.0051 | 0.0069 | 0.0041 | 0.008 | 0.0055 | 0.0091 | 0.0266 | 0.0042 | 0.0054 | 0.0081 | 0.0063 | 0.009 | 0.0084 | 0.0138 | 0.0042 |
Vacuole Periphery | 0.002 | 0.0011 | 0.0032 | 0.0027 | 0.0024 | 0.001 | 0.0033 | 0.0013 | 0.0024 | 0.0097 | 0.0013 | 0.0018 | 0.0018 | 0.0015 | 0.0022 | 0.0033 | 0.0029 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.2805 | 5.3362 | 7.3102 | 8.5188 | 7.1441 | 6.4249 | 11.6209 | 9.6641 | 9.7967 | 11.2052 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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