Standard name
Human Ortholog
Description Protein involved in 2'-O-methylation of C32 of substrate tRNAs; interacts with 2'-O-ribose methyltransferase Trm7p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene; yeast null mutant can be functionally complemented by human THADA, mutations in which have been implicated in epithelial thyroid adenomas, type 2 diabetes, and polycystic ovary syndrome (PCOS)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.98 0.95 0.94 0.97 0.97 0.95 0.89 0.89 0.85 – 0.75 0.72 0.99 1.0 0.99 0.91 0.9 0.95 0.97 0.92 0.85 0.98 0.96 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.08 0.06 0.18 0.23 0.24 0.34 – 0.47 0.51 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 1 1 4 3 5 5 6 3 – 10 13 0 0 7 0 0 0 0 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 3 3 1 11 13 12 8 7 – 6 10 2 4 14 16 8 9 0 0 0 0 0 0
Cytoplasm 165 143 100 205 405 368 476 476 258 – 219 360 143 312 382 129 97 101 168 136 92 106 314 335
Endoplasmic Reticulum 1 4 1 0 2 2 1 1 0 – 0 0 2 0 1 6 1 4 2 1 2 0 4 11
Endosome 0 0 0 0 2 0 3 2 1 – 1 0 0 0 0 0 1 0 0 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0 0 0 1
Mitochondria 0 2 13 17 27 69 121 127 102 – 138 258 0 0 5 1 2 0 0 2 3 0 0 3
Nucleus 0 0 1 0 1 1 0 2 0 – 2 0 0 2 0 7 3 7 0 0 2 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 1 2 2 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 2 2 1 0 5 4 4 6 3 – 7 7 0 0 0 2 0 2 0 3 2 0 2 1
Unique Cell Count 168 150 106 211 418 386 534 533 304 292 501 144 313 386 141 108 106 173 149 109 109 329 364
Labelled Cell Count 175 157 121 230 458 462 626 631 376 383 649 147 318 410 162 112 123 173 149 109 109 329 364


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 8.1 7.1 6.8 6.1 5.4 4.7 4.8 4.6 – 4.2 3.8 9.1 8.1 6.9 10.2 11.5 12.2 8.9 8.7 9.2
Std Deviation (1e-4) 1.2 1.2 3.0 2.5 1.4 1.5 1.0 1.5 2.6 – 1.4 1.0 1.2 1.0 0.9 1.5 1.4 1.9 1.2 1.3 1.3
Intensity Change (Log2) – – – -0.06 -0.21 -0.39 -0.59 -0.55 -0.63 – -0.75 -0.91 0.36 0.2 -0.04 0.53 0.7 0.78 0.33 0.29 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 2.5 1.5 1.8
Cytoplasm 1.2 1.3 0.4 -1.6 -1.6 -2.5 – -4.3 -4.9 2.4 3.7 3.0 -0.9 -1.2 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -1.2 -2.0 1.4 0 0 0 – 0 0 -4.3 0 0 -3.9 -3.0 -3.7
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9181 7.056 6.1457 5.5293 5.8112 6.1307 4.046 6.7034 5.9866 5.3506 4.9421 5.5745 2.1645 3.1332 2.5149 2.9927 2.8345 2.802
Actin 0.0209 0.002 0.0035 0.0074 0.0284 0.0158 0.1089 0.007 0.0003 0.0359 0.01 0.0005 0.0128 0.0004 0.0414 0.0101 0.0005 0.0002
Bud 0.0009 0.0006 0.0026 0.0008 0.0011 0.0041 0.0007 0.0087 0.0002 0.0005 0.0005 0.0004 0.0014 0.0002 0.0006 0.0007 0.0003 0.0002
Bud Neck 0.006 0.0005 0.001 0.0035 0.0058 0.004 0.0006 0.0002 0.0001 0.0008 0.0004 0.0006 0.0018 0.0001 0.0008 0.0005 0.0004 0.0014
Bud Periphery 0.0016 0.0005 0.0027 0.0009 0.0023 0.0056 0.0006 0.0018 0 0.0002 0.0004 0.0001 0.0015 0 0.0005 0.0003 0.0001 0.0001
Bud Site 0.0119 0.0042 0.0086 0.0032 0.012 0.0013 0.0023 0.0036 0.0002 0.0008 0.0006 0.0001 0.0037 0.0013 0.0035 0.0004 0.0002 0.0002
Cell Periphery 0.0021 0.0012 0.0015 0.001 0.0015 0.0007 0.0002 0.0002 0 0.0001 0.0001 0 0.0013 0.0002 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.752 0.9454 0.8996 0.8283 0.8083 0.9122 0.7095 0.9653 0.9657 0.8711 0.876 0.9361 0.5706 0.846 0.8566 0.7369 0.9405 0.9382
Cytoplasmic Foci 0.014 0.0092 0.0103 0.0206 0.0118 0.0123 0.0265 0.0029 0.0052 0.0275 0.0182 0.0099 0.0449 0.0035 0.0112 0.0303 0.0152 0.0097
Eisosomes 0.0012 0 0.0001 0.0002 0.0007 0.0001 0.0004 0.0001 0 0.0001 0.0001 0 0.003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.029 0.0044 0.0104 0.0345 0.0028 0.0104 0.0107 0.0021 0.0031 0.0037 0.0062 0.0091 0.0923 0.0012 0.005 0.0124 0.0055 0.003
Endosome 0.035 0.0085 0.0115 0.0324 0.0344 0.0094 0.0359 0.0023 0.0028 0.0321 0.022 0.0276 0.1021 0.0013 0.0106 0.0578 0.0243 0.0175
Golgi 0.0082 0.0035 0.0018 0.0145 0.0133 0.0047 0.0165 0.0005 0.0008 0.0059 0.0414 0.0015 0.0308 0.0002 0.002 0.0145 0.0008 0.0005
Lipid Particles 0.0164 0.0004 0.0007 0.0072 0.0064 0.002 0.0103 0.0005 0.0008 0.0023 0.0023 0.0003 0.0401 0.0001 0.0009 0.0006 0.0001 0.0001
Mitochondria 0.0241 0.0054 0.0112 0.0066 0.0533 0.0048 0.011 0.0003 0.0002 0.0013 0.0117 0.0007 0.0161 0 0.0249 0.001 0.0002 0.0002
None 0.0349 0.005 0.0178 0.0085 0.0029 0.0056 0.0287 0.0023 0.0042 0.0032 0.0027 0.0016 0.0389 0.1433 0.0323 0.1224 0.0046 0.0238
Nuclear Periphery 0.0152 0.0004 0.0052 0.003 0.0011 0.0005 0.0046 0.0003 0.0004 0.0011 0.0009 0.0005 0.0067 0.0002 0.0009 0.0021 0.0011 0.0004
Nucleolus 0.001 0.0001 0.0004 0.0005 0.0006 0.0001 0.0002 0 0 0 0 0 0.002 0 0.0001 0 0 0
Nucleus 0.0039 0.0008 0.0029 0.0023 0.0012 0.0008 0.0031 0.0006 0.0005 0.0008 0.0008 0.0005 0.0021 0.0004 0.0011 0.001 0.0011 0.0007
Peroxisomes 0.0044 0.0029 0.0004 0.0098 0.0037 0.0019 0.0197 0.0003 0.0138 0.0049 0.0035 0.0002 0.0178 0 0.0023 0.0004 0.0001 0
Punctate Nuclear 0.0039 0.0001 0.0009 0.002 0.0004 0.0005 0.0039 0.0001 0.0001 0.0014 0.0003 0.0001 0.0021 0.0001 0.0005 0.0004 0.0003 0.0002
Vacuole 0.0107 0.0045 0.0059 0.0116 0.0052 0.0028 0.0047 0.0007 0.0013 0.0052 0.0017 0.0079 0.006 0.0011 0.0036 0.0054 0.0039 0.0032
Vacuole Periphery 0.0029 0.0005 0.0013 0.0012 0.0027 0.0006 0.0009 0.0001 0.0002 0.0012 0.0005 0.0023 0.002 0.0001 0.0007 0.0026 0.0009 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.6951 22.2126 24.5523 23.5213 21.6454 16.8148 24.974 20.448 16.0311 26.641
Translational Efficiency 0.642 0.6999 0.5803 0.5536 0.5453 0.6079 0.5132 0.4269 0.5817 0.4734

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
637 1007 209 643 600 1281 182 529 1237 2288 391 1172

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 825.50 1020.10 1357.21 1066.65 835.75 902.60 1202.99 1055.25 830.47 954.31 1285.42 1061.50
Standard Deviation 106.66 132.18 165.92 133.37 129.03 125.43 125.44 139.54 118.15 141.07 167.20 136.31
Intensity Change Log 2 — 0.305370 0.717304 0.369747 — 0.111015 0.525481 0.336441 — 0.210864 0.623987 0.353087

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000208 0.000229 0.000671 0.004647 0.000311 0.000607 0.000751 0.000167 0.000258 0.000441 0.000708 0.002625
Bud Neck 0.001893 0.002394 0.004038 0.002692 0.002714 0.003420 0.001863 0.001483 0.002291 0.002968 0.003026 0.002146
Bud Site 0.015568 0.024940 0.034670 0.045822 0.022345 0.033867 0.038075 0.018315 0.018855 0.029938 0.036255 0.033407
Cell Periphery 0.000242 0.000131 0.000251 0.000146 0.000220 0.000127 0.000180 0.000063 0.000231 0.000129 0.000218 0.000108
Cytoplasm 0.713824* 0.740791* 0.641686* 0.728792* 0.651317* 0.724087* 0.589226* 0.729631* 0.683505* 0.731439* 0.617267* 0.729171*
Cytoplasmic Foci 0.185042* 0.158117 0.056532 0.061492 0.200353* 0.143307 0.099869 0.068331 0.192468* 0.149825 0.076704 0.064579
Eisosomes 0.000046 0.000016 0.000028 0.000025 0.000037 0.000028 0.000037 0.000008 0.000042 0.000022 0.000032 0.000018
Endoplasmic Reticulum 0.006001 0.001895 0.001326 0.000477 0.003312 0.000799 0.000770 0.000215 0.004697 0.001281 0.001067 0.000359
Endosome 0.010995 0.010250 0.009874 0.010460 0.022964 0.015794 0.009731 0.005429 0.016801 0.013354 0.009808 0.008190
Golgi 0.007238 0.008186 0.000573 0.017555 0.008032 0.010449 0.000447 0.000664 0.007624 0.009453 0.000514 0.009931
Lipid Particles 0.002477 0.001029 0.002229 0.000443 0.002796 0.000726 0.001498 0.000453 0.002631 0.000860 0.001889 0.000447
Mitochondria 0.000757 0.000445 0.000400 0.010071 0.000610 0.002467 0.000019 0.002185 0.000686 0.001577 0.000223 0.006511
Mitotic Spindle 0.000882 0.002346 0.007985 0.020129 0.001579 0.004706 0.001344 0.013513 0.001220 0.003667 0.004893 0.017143
None 0.002080 0.000504 0.001249 0.001056 0.001185 0.000471 0.000684 0.000299 0.001645 0.000486 0.000986 0.000714
Nuclear Periphery 0.000069 0.000026 0.000385 0.000069 0.000199 0.000022 0.000165 0.000137 0.000132 0.000023 0.000282 0.000100
Nuclear Periphery Foci 0.000936 0.000515 0.015736 0.001107 0.001083 0.000227 0.009768 0.001023 0.001007 0.000354 0.012958 0.001069
Nucleolus 0.000222 0.000094 0.000086 0.000074 0.000266 0.000202 0.000060 0.000099 0.000243 0.000154 0.000074 0.000085
Nucleus 0.009600 0.003708 0.036825 0.007680 0.009439 0.003892 0.016391 0.005761 0.009522 0.003811 0.027313 0.006814
Peroxisomes 0.000955 0.001397 0.000324 0.002142 0.001023 0.001760 0.001048 0.000912 0.000988 0.001600 0.000661 0.001587
Vacuole 0.036452 0.039860 0.184585 0.081995 0.064943 0.049125 0.227829* 0.149213 0.050271 0.045047 0.204714* 0.112335
Vacuole Periphery 0.004514 0.003128 0.000548 0.003125 0.005271 0.003917 0.000248 0.002097 0.004881 0.003570 0.000408 0.002661

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.56 -7.68 -3.29 -3.26 -2.38 -1.03 -2.71 1.63 1.63 5.89 -1.15 -6.31 -3.03 -2.21 -1.68
Bud Neck -0.92 -1.74 -0.33 0.67 1.54 -1.73 3.13 4.84 5.84 2.73 -2.09 -0.71 2.74 3.79 2.02
Bud Site -4.84 -1.69 -3.31 -0.51 -0.38 -4.32 -1.21 3.03 7.45 2.46 -6.58 -2.03 -1.34 3.61 1.19
Cell Periphery 5.39 0.28 7.42 2.44 7.15 4.61 2.81 9.08 7.02 8.88 7.14 1.88 11.38 5.75 10.32
Cytoplasm -2.29 7.71 2.77 4.89 -5.54 -5.79 4.74 -2.11 3.07 -6.09 -5.57 8.79 0.47 5.56 -8.18
Cytoplasmic Foci 3.22 18.20 18.14 16.42 -1.61 6.66 12.12 17.16 14.50 3.53 7.16 20.98 24.96 22.04 1.67
Eisosomes 7.43 3.25 6.44 -1.05 4.72 2.59 0.47 10.33 7.08 10.61 6.78 2.90 10.60 4.58 10.05
Endoplasmic Reticulum 2.88 3.78 4.55 2.39 7.24 2.97 3.18 3.84 3.18 5.52 4.15 4.87 5.88 3.32 8.90
Endosome 0.60 2.12 3.82 3.68 2.05 3.19 7.27 9.39 10.36 3.89 2.66 7.16 9.56 9.36 3.67
Golgi -1.03 13.50 0.13 0.51 -2.90 -2.10 11.34 11.16 10.95 -0.38 -2.46 17.23 2.83 4.00 -2.91
Lipid Particles 4.03 0.20 5.01 1.86 6.95 3.94 2.33 4.37 2.84 6.76 5.66 1.89 6.42 2.67 9.23
Mitochondria 1.11 1.78 -1.88 -2.08 -2.22 -2.15 3.94 -0.48 0.99 -1.05 -1.76 3.22 -1.89 -0.97 -2.45
Mitotic Spindle -1.17 -1.92 -3.54 -2.92 -1.61 -1.62 -0.41 -2.03 -1.14 -1.98 -2.09 -1.86 -3.92 -2.76 -2.51
None 3.79 2.59 2.45 -1.41 0.10 1.49 1.20 1.89 2.39 2.70 3.68 2.63 2.99 -0.87 0.86
Nuclear Periphery 2.95 -12.01 -4.75 -8.28 9.49 2.23 -0.64 0.78 -2.89 2.76 2.76 -6.26 -0.42 -6.57 8.67
Nuclear Periphery Foci 2.20 -11.89 -2.87 -4.35 11.33 6.13 -10.02 -0.01 -8.47 10.09 5.85 -15.04 -2.34 -7.71 14.57
Nucleolus 1.50 1.53 1.54 0.33 0.13 0.38 3.12 2.70 1.12 -2.84 0.96 3.07 2.84 1.13 -1.90
Nucleus 7.56 -8.66 -4.81 -9.65 6.68 3.34 -3.47 0.54 -6.28 4.29 6.27 -8.88 -3.01 -11.20 7.65
Peroxisomes -1.32 2.07 -0.90 0.53 -4.93 -3.70 1.25 1.78 5.86 0.16 -3.06 2.47 -0.21 3.63 -3.67
Vacuole -1.23 -14.18 -16.74 -16.45 5.13 3.24 -12.23 -13.60 -15.96 3.92 1.68 -18.68 -21.21 -22.70 6.46
Vacuole Periphery 1.96 6.23 3.76 2.95 -1.61 1.79 8.14 4.78 3.78 -1.53 2.50 10.05 6.01 4.82 -2.23
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein involved in 2'-O-methylation of C32 of substrate tRNAs; interacts with 2'-O-ribose methyltransferase Trm7p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene; yeast null mutant can be functionally complemented by human THADA, mutations in which have been implicated in epithelial thyroid adenomas, type 2 diabetes, and polycystic ovary syndrome (PCOS)
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Trm732

Trm732


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm732-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available