Standard name
Human Ortholog
Description Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.92 0.93 0.95 0.95 0.93 0.84 0.77 0.72 0.62 0.68 0.58 0.55 0.98 0.97 0.97 0.97 0.92 0.94 0.45 0.68 0.5 0.64 0.71 0.73
Endoplasmic Reticulum 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.19 0.11 0.2 0.05 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.2 0 0.07 0.1 0.14 0.36 0.43 0.5 0.59 0.49 0.6 0.63 0 0 0 0 0 0 0.26 0.1 0.16 0.21 0.1 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 2 0 0 0 0 0 0 0 1 0 0 1 0 0 7 3 0 2 2
Bud 0 0 1 2 4 12 13 11 15 7 21 17 0 1 0 0 0 0 0 2 1 0 2 1
Bud Neck 0 0 0 0 1 2 0 2 2 0 0 0 0 2 0 1 0 0 0 7 1 0 3 1
Bud Site 0 0 0 0 0 0 2 1 4 3 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 8 2 2 1 3 3 1 0 4 1 1 1 12 1 1 7 6 0 7 3 2 2 3
Cytoplasm 56 314 132 216 359 334 438 398 277 359 226 225 120 223 218 123 153 118 38 250 73 78 143 175
Endoplasmic Reticulum 1 19 2 0 1 0 0 0 3 0 2 3 2 4 5 6 5 8 16 41 29 6 7 4
Endosome 0 0 0 0 2 0 1 3 1 9 2 1 0 0 1 0 1 0 1 1 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 1 0 0 4 1
Mitochondria 12 5 10 23 53 141 247 276 264 260 234 259 0 0 1 5 5 5 22 35 23 25 19 32
Nucleus 0 0 1 2 1 2 2 4 0 1 3 1 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 1 0 1 1 0 0 4 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 2 0 0 0 0 2 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 0 0 6 1 2 3 4 13 6 8 0 0 0 0 0 0 0 0 0 0 1 3
Vac/Vac Membrane 1 2 1 1 1 5 2 5 14 7 5 4 0 0 0 1 3 0 2 8 4 4 9 10
Unique Cell Count 61 337 139 227 387 397 571 549 446 531 387 408 122 229 225 127 166 126 87 368 147 122 201 241
Labelled Cell Count 71 349 149 247 430 502 714 705 584 663 508 526 123 243 226 138 179 141 87 368 147 122 201 241


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.5 3.7 3.7 3.7 3.5 3.2 3.3 3.1 3.0 3.0 2.9 4.9 4.8 5.2 5.2 5.6 5.4 5.2 5.5 5.4
Std Deviation (1e-4) 0.5 0.6 1.0 1.5 1.7 1.6 1.5 1.4 1.3 1.1 1.1 0.9 0.8 0.7 1.2 1.4 2.2 1.6 1.5 1.8 1.2
Intensity Change (Log2) – – – -0.0 0.02 -0.06 -0.23 -0.17 -0.23 -0.31 -0.31 -0.34 0.41 0.39 0.5 0.5 0.61 0.55 0.5 0.57 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0681 0.0003 0.0073 0.0007 0.001 0.0049 0.0365 0.0002 0.0058 0.0008 0.0277 0.003 0.0367 0.0102 0.0001 0 0 0.004
Bud 0.0025 0.0023 0.005 0.0015 0.0116 0.0032 0.0012 0.0007 0.0012 0.002 0.0007 0.0002 0.0008 0.0007 0 0 0 0.0002
Bud Neck 0.0104 0.0003 0.001 0.0004 0.0014 0.0024 0.0112 0.0002 0.0069 0.0016 0.0005 0.0004 0.0019 0.0006 0 0 0 0.0007
Bud Periphery 0.0097 0.0014 0.0032 0.0011 0.0035 0.0012 0.0058 0.0011 0.0041 0.0139 0.0012 0.0005 0.0012 0.0006 0 0 0 0.0004
Bud Site 0.0059 0.0146 0.0046 0.004 0.0028 0.0005 0.011 0.019 0.008 0.0102 0.0028 0.0002 0.006 0.0254 0 0 0 0.0002
Cell Periphery 0.0006 0.0004 0.0003 0.0006 0.0002 0.0001 0.0021 0.0007 0.0008 0.0004 0.0001 0.0001 0.0015 0.0003 0.0001 0 0 0.0001
Cytoplasm 0.0289 0.0308 0.0286 0.0787 0.0372 0.056 0.0386 0.0728 0.0674 0.0318 0.0501 0.0949 0.1172 0.0757 0.0981 0.0045 0.0009 0.1681
Cytoplasmic Foci 0.0285 0.0051 0.0159 0.0302 0.0241 0.0115 0.0266 0.0036 0.0115 0.0098 0.0338 0.0087 0.006 0.0246 0.0016 0.0005 0.0003 0.0236
Eisosomes 0.0013 0.0003 0.0005 0.0003 0.0003 0.0003 0.0008 0.0006 0.0007 0.0003 0.0003 0.0002 0.0025 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.007 0.0008 0.0011 0.0054 0.0005 0.006 0.0057 0.0007 0.0044 0.0006 0.0014 0.0012 0.0314 0.0021 0.0028 0.0001 0 0.0009
Endosome 0.0222 0.0004 0.0061 0.0062 0.0076 0.0023 0.0302 0.0006 0.0065 0.0029 0.0358 0.0042 0.0355 0.005 0.0005 0 0 0.0054
Golgi 0.0054 0.0001 0.0021 0.0005 0.0034 0.001 0.0099 0.0001 0.0016 0.0012 0.039 0.003 0.0025 0.0095 0 0 0 0.0107
Lipid Particles 0.0167 0.0004 0.0117 0.0068 0.0236 0.0031 0.022 0.0002 0.008 0.0128 0.0321 0.0047 0.0041 0.0022 0.0002 0.0001 0 0.0411
Mitochondria 0.0084 0.0006 0.0027 0.0012 0.0037 0.0008 0.0177 0.0002 0.0097 0.004 0.017 0.0063 0.0077 0.0036 0.0001 0 0 0.0068
None 0.7041 0.9281 0.8833 0.8177 0.7566 0.8775 0.7303 0.8969 0.8424 0.8739 0.6932 0.8619 0.3397 0.8299 0.895 0.9944 0.9985 0.7321
Nuclear Periphery 0.0213 0.0006 0.0024 0.0054 0.0024 0.0076 0.0049 0.0001 0.0055 0.0026 0.0021 0.0033 0.1549 0.0002 0.0004 0 0 0.0006
Nucleolus 0.0041 0.0015 0.0014 0.0032 0.0144 0.0012 0.0033 0.0003 0.0009 0.0016 0.0003 0.0003 0.0128 0.0001 0 0 0 0.0001
Nucleus 0.0072 0.0031 0.003 0.0065 0.0265 0.0046 0.0087 0.0008 0.002 0.0113 0.0004 0.0008 0.1767 0.0002 0.0003 0 0 0.0003
Peroxisomes 0.0154 0.0002 0.0137 0.002 0.0261 0.0033 0.0237 0.0001 0.0084 0.0128 0.0539 0.0028 0.0012 0.0079 0 0 0 0.0034
Punctate Nuclear 0.0213 0.0078 0.0052 0.0235 0.0515 0.0115 0.0045 0.0005 0.0021 0.0031 0.005 0.0021 0.0435 0.0003 0.0002 0 0.0001 0.0005
Vacuole 0.0061 0.0007 0.001 0.0036 0.0012 0.0005 0.0039 0.0005 0.0016 0.0021 0.0011 0.0005 0.0103 0.0005 0.0004 0.0001 0 0.0004
Vacuole Periphery 0.0049 0.0001 0.0003 0.0007 0.0004 0.0004 0.0013 0 0.0005 0.0003 0.0013 0.0005 0.0059 0.0001 0 0 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.3002 43.5166 52.6227 55.1539 47.9208 75.6819 65.592 57.1937 76.6461 64.0303
Translational Efficiency 1.0801 1.0033 1.0 0.9916 1.0846 0.7303 0.7716 0.9943 0.7793 0.7286

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation
Localization
Cell Percentages cytoplasm (32%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Cue1

Cue1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cue1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available