Standard name
Human Ortholog
Description Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.56 0.47 0.65 0.65 0.61 0.6 0.6 0.52 – 0.56 0.52 0.51 0.5 0.39 0.27 0.38 0.43 0.51 0.08 0.1 0.13 0.07 0.06 0.05
Bud 0.12 0.1 0.14 0.12 0.13 0.12 0.14 0.13 – 0.12 0.15 0.14 0.05 0.08 0 0.06 0.1 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.69 0.73 0.62 0.69 0.6 0.56 0.51 0.37 – 0.47 0.5 0.48 0.81 0.77 0.87 0.25 0.32 0.45 0.81 0.76 0.72 0.78 0.81 0.77
Cytoplasm 0 0.11 0.06 0.06 0.08 0.06 0.07 0.12 – 0.12 0.14 0.14 0 0.1 0.09 0.38 0.24 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.08 0.06 0.12 0.07 0.11 0.17 0.16 0.25 – 0.21 0.19 0.21 0.1 0.07 0 0.05 0 0 0.05 0 0.07 0.06 0.07 0.12
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.07 0.11 0.07 0.07 0.11 0.13 – 0.15 0.17 0.13 0 0 0 0.1 0.1 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0.05 0.1 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 151 73 201 159 224 177 293 259 – 247 187 219 114 112 62 101 255 146 23 17 46 19 21 24
Bud 32 16 44 30 48 34 67 64 – 54 53 59 11 22 10 15 59 32 1 1 1 2 0 5
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 1 0 8 4 6 6 4 5
Bud Site 3 0 2 1 0 1 1 2 – 2 0 1 1 0 0 1 0 0 – – – – – –
Cell Periphery 185 113 189 169 221 165 251 184 – 211 182 207 185 219 202 68 193 129 248 137 262 219 299 375
Cytoplasm 12 17 18 15 28 19 36 60 – 53 49 62 9 28 20 103 145 49 1 6 3 1 0 1
Endoplasmic Reticulum 22 10 38 17 39 51 77 126 – 95 69 90 22 20 8 14 15 6 15 8 25 18 25 56
Endosome 0 0 1 0 0 0 1 1 – 1 0 0 0 1 1 0 0 0 0 0 4 0 0 0
Golgi 0 0 1 0 8 0 2 1 – 4 0 1 2 0 0 3 11 11 2 0 1 0 1 1
Mitochondria 11 7 21 26 25 21 54 63 – 68 60 55 0 0 0 28 62 23 4 0 8 6 10 6
Nucleus 0 0 0 1 0 2 0 0 – 0 0 0 0 1 0 5 5 1 0 0 0 0 0 0
Nuclear Periphery 0 0 1 1 0 1 1 3 – 4 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 2 0 8 5 18 14 47 20 – 26 7 6 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 4 0 0 0 0 0 1 0 – 2 0 0 0 0 1 6 6 5 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 1 – 1 1 3 0 1 0 4 8 2 2 1 0 4 2 4
Unique Cell Count 270 155 307 245 367 293 488 495 445 362 431 228 286 232 268 599 286 309 180 364 281 369 486
Labelled Cell Count 422 237 524 424 611 485 832 784 768 609 703 344 404 304 350 760 406 309 180 364 281 369 486


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 15.5 12.3 13.8 13.6 12.0 11.4 11.6 – 11.2 11.6 11.9 17.5 14.8 14.9 8.6 10.7 13.5 20.5 21.4 21.1
Std Deviation (1e-4) 3.3 3.7 3.0 2.8 2.9 2.7 2.7 2.9 – 3.1 2.9 2.9 3.5 3.3 2.9 2.1 2.6 3.1 4.5 4.4 4.4
Intensity Change (Log2) – – – 0.16 0.14 -0.04 -0.11 -0.08 – -0.14 -0.08 -0.05 0.51 0.26 0.28 -0.51 -0.2 0.13 0.74 0.8 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -1.2 -1.3 -1.5 -3.7 – -2.7 -3.6 -4.0 -3.6 -6.4 -8.9 -6.7 -6.5 -3.6
Bud -0.7 -0.5 -1.0 -0.2 -0.6 – -0.9 0.1 -0.2 -3.6 -2.6 -3.8 -3.4 -2.0 -1.1
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 -0.4 -1.3 -2.8 -6.7 – -3.8 -2.9 -3.6 4.9 3.9 6.6 -8.7 -8.5 -4.0
Cytoplasm 0.1 0.9 0.3 0.8 2.9 – 2.8 3.3 3.7 -1.0 1.8 1.2 9.6 6.8 4.3
Endoplasmic Reticulum -2.1 -0.7 1.7 1.3 4.5 – 3.2 2.3 3.0 -1.0 -2.2 -3.7 -3.0 -6.0 -4.8
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 3.0
Mitochondria 1.6 0 0.2 2.0 2.6 – 3.5 3.8 2.6 -4.0 -4.5 -4.1 1.5 1.7 0.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes -0.4 1.5 1.4 3.8 1.1 – 2.1 -0.6 -1.2 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0909 0.0208 0.0565 0.0388 0.141 0.0252 – – – – – – – – – – – –
Bud 0.0044 0.0024 0.005 0.0071 0.0062 0.0022 – – – – – – – – – – – –
Bud Neck 0.0159 0.007 0.0354 0.1068 0.0436 0.0687 – – – – – – – – – – – –
Bud Periphery 0.0219 0.0135 0.0342 0.0462 0.0587 0.0288 – – – – – – – – – – – –
Bud Site 0.0783 0.1722 0.1252 0.058 0.0089 0.0068 – – – – – – – – – – – –
Cell Periphery 0.3975 0.4605 0.4734 0.5099 0.3586 0.6549 – – – – – – – – – – – –
Cytoplasm 0.0782 0.1869 0.1282 0.1045 0.1414 0.1262 – – – – – – – – – – – –
Cytoplasmic Foci 0.018 0.0031 0.0101 0.0143 0.0212 0.0027 – – – – – – – – – – – –
Eisosomes 0.0282 0.0176 0.0074 0.0177 0.0408 0.006 – – – – – – – – – – – –
Endoplasmic Reticulum 0.0344 0.0329 0.0203 0.024 0.0335 0.0231 – – – – – – – – – – – –
Endosome 0.0746 0.0082 0.01 0.0055 0.005 0.0034 – – – – – – – – – – – –
Golgi 0.0254 0.0059 0.007 0.0055 0.0199 0.0029 – – – – – – – – – – – –
Lipid Particles 0.0203 0.0126 0.0211 0.012 0.012 0.01 – – – – – – – – – – – –
Mitochondria 0.0184 0.0164 0.028 0.0023 0.0771 0.0014 – – – – – – – – – – – –
None 0.0082 0.0016 0.0026 0.0019 0.0014 0.0008 – – – – – – – – – – – –
Nuclear Periphery 0.0197 0.0042 0.0019 0.0015 0.0013 0.0077 – – – – – – – – – – – –
Nucleolus 0.0025 0.001 0.0005 0.0012 0.0003 0.0005 – – – – – – – – – – – –
Nucleus 0.0226 0.003 0.0011 0.0013 0.0006 0.0011 – – – – – – – – – – – –
Peroxisomes 0.0119 0.0003 0.0031 0.0123 0.0015 0.0002 – – – – – – – – – – – –
Punctate Nuclear 0.0071 0.0004 0.0006 0.0004 0.0003 0.0002 – – – – – – – – – – – –
Vacuole 0.0169 0.0269 0.0272 0.028 0.026 0.0256 – – – – – – – – – – – –
Vacuole Periphery 0.0046 0.0025 0.0008 0.0009 0.0008 0.0015 – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.7703 37.911 32.4792 41.3739 42.9883 46.624 37.4606 37.1607 31.1835 41.076
Translational Efficiency 0.7629 0.7911 0.79 0.5941 0.7092 0.7836 0.6318 0.5582 0.6175 0.5539

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
200 449 338 895 1419 221 1372 998 1619 670 1710 1893

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1205.47 1353.29 1354.47 1268.02 1168.58 1271.56 1240.97 1273.07 1173.14 1326.33 1263.40 1270.68
Standard Deviation 205.97 242.24 189.13 245.74 257.38 196.84 201.66 221.02 251.90 231.47 204.31 233.05
Intensity Change Log 2 — 0.166875 0.168133 0.072982 — 0.121843 0.086712 0.123555 — 0.144885 0.128629 0.098097

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.042868 0.023443 0.038982 0.015835 0.004490 0.017005 0.041957 0.016448 0.009231 0.021319 0.041369 0.016158
Bud Neck 0.034628 0.012600 0.038157 0.012479 0.009081 0.009123 0.022892 0.011524 0.012237 0.011453 0.025909 0.011976
Bud Site 0.073410 0.034485 0.048245 0.040730 0.015508 0.027362 0.057353 0.031362 0.022660 0.032135 0.055552 0.035791
Cell Periphery 0.470814* 0.849892* 0.693157* 0.836134* 0.729846* 0.839591* 0.692138* 0.872030* 0.697847* 0.846494* 0.692339* 0.855058*
Cytoplasm 0.092969 0.009246 0.007749 0.019967 0.027766 0.017096 0.053638 0.016786 0.035820 0.011835 0.044568 0.018290
Cytoplasmic Foci 0.018567 0.002017 0.000869 0.001144 0.001478 0.003611 0.003863 0.001080 0.003589 0.002543 0.003271 0.001110
Eisosomes 0.023747 0.023990 0.049252 0.019369 0.005109 0.018916 0.030212 0.012928 0.007411 0.022316 0.033975 0.015973
Endoplasmic Reticulum 0.034833 0.026887 0.080222 0.015083 0.045980 0.032156 0.036982 0.014139 0.044603 0.028625 0.045529 0.014585
Endosome 0.013169 0.002559 0.002392 0.002924 0.003293 0.005377 0.003887 0.001623 0.004513 0.003489 0.003592 0.002238
Golgi 0.003511 0.000987 0.001635 0.003007 0.000735 0.004373 0.002360 0.002118 0.001078 0.002104 0.002217 0.002538
Lipid Particles 0.016917 0.003215 0.012744 0.004195 0.005346 0.005656 0.018142 0.005256 0.006775 0.004020 0.017075 0.004754
Mitochondria 0.000348 0.000883 0.000298 0.002363 0.001018 0.002800 0.001039 0.001371 0.000935 0.001516 0.000893 0.001840
Mitotic Spindle 0.000365 0.001131 0.001102 0.001975 0.000333 0.001028 0.000736 0.000779 0.000337 0.001097 0.000808 0.001345
None 0.000016 0.000043 0.000053 0.000073 0.000059 0.000025 0.000079 0.000033 0.000053 0.000037 0.000074 0.000052
Nuclear Periphery 0.000210 0.000934 0.000802 0.000835 0.001171 0.000756 0.000849 0.000892 0.001052 0.000875 0.000840 0.000865
Nuclear Periphery Foci 0.001731 0.000274 0.000927 0.000658 0.000519 0.000498 0.000548 0.000308 0.000669 0.000348 0.000623 0.000473
Nucleolus 0.000025 0.000015 0.000011 0.000028 0.000098 0.000014 0.000032 0.000015 0.000089 0.000014 0.000028 0.000021
Nucleus 0.002706 0.000265 0.000927 0.000640 0.001257 0.000229 0.002012 0.000319 0.001436 0.000253 0.001798 0.000471
Peroxisomes 0.002705 0.004042 0.002038 0.001466 0.010859 0.007009 0.010493 0.002522 0.009852 0.005021 0.008822 0.002023
Vacuole 0.165657 0.002885 0.020240 0.019169 0.135025 0.006252 0.020424 0.008171 0.138810 0.003996 0.020388 0.013371
Vacuole Periphery 0.000805 0.000209 0.000199 0.001928 0.001030 0.001124 0.000363 0.000295 0.001002 0.000511 0.000330 0.001067

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.28 0.41 3.45 1.81 4.00 -2.67 -12.25 -5.75 0.11 7.14 -3.83 -11.24 -3.91 1.58 8.52
Bud Neck 3.96 -0.57 4.05 0.06 7.09 -0.03 -8.29 -1.83 -1.55 6.46 0.51 -8.13 0.18 -0.39 9.04
Bud Site 3.53 2.28 3.16 -0.98 1.19 -1.50 -10.84 -4.29 -0.48 5.57 -1.94 -9.22 -4.14 -0.74 5.23
Cell Periphery -12.86 -7.07 -12.75 1.08 -8.61 -6.00 3.56 -15.16 -1.77 -16.36 -12.98 0.67 -18.02 -0.80 -17.74
Cytoplasm 8.10 8.31 6.97 -3.82 -4.91 2.39 -6.77 3.51 0.08 10.10 8.02 -2.58 6.00 -2.87 9.05
Cytoplasmic Foci 4.02 4.54 4.49 0.57 -0.42 -1.60 -3.33 0.98 1.92 3.89 0.90 0.35 4.12 1.31 3.84
Eisosomes -0.09 -4.28 1.00 1.72 6.24 -4.53 -14.93 -7.17 1.93 9.67 -7.77 -15.46 -7.43 3.22 10.14
Endoplasmic Reticulum 1.08 -4.14 2.98 2.77 7.01 2.15 2.27 9.21 3.04 7.60 3.83 -0.25 9.79 4.15 10.24
Endosome 3.58 3.64 3.35 -0.46 -0.66 -1.37 -0.91 3.74 2.55 4.21 1.43 1.44 3.55 1.90 2.41
Golgi 2.74 2.02 0.32 -1.71 -1.16 -2.98 -4.12 -1.36 1.43 0.22 -2.29 -3.33 -1.86 -0.51 -0.41
Lipid Particles 3.66 0.91 3.54 -0.63 3.13 -0.13 -6.14 0.07 0.16 5.62 1.93 -5.64 1.74 -0.53 6.89
Mitochondria -2.72 0.46 -1.97 -1.43 -2.02 -0.79 -0.11 -0.72 0.62 -0.70 -0.76 0.31 -1.63 -0.35 -1.74
Mitotic Spindle -4.65 -4.47 -1.96 -1.03 -1.08 -4.67 -3.51 -6.07 1.61 -0.37 -7.46 -4.86 -2.58 -0.63 -1.37
None -3.51 -3.99 -3.90 -1.96 -1.33 1.47 -0.85 1.22 -0.84 5.17 0.84 -0.98 0.06 -1.72 2.12
Nuclear Periphery -6.94 -6.69 -8.88 0.82 -0.33 2.95 3.13 2.36 -0.93 -0.40 1.70 2.40 2.11 0.10 -0.34
Nuclear Periphery Foci 6.42 3.11 3.81 -2.32 1.63 0.29 -0.52 4.23 2.49 5.14 5.82 0.59 2.08 -1.52 1.80
Nucleolus 1.75 2.48 -0.31 -2.74 -3.88 5.29 4.44 5.55 -0.16 9.05 5.62 4.69 5.13 -2.13 2.65
Nucleus 4.79 3.24 4.05 -3.20 2.22 1.89 -1.46 1.73 -1.62 8.24 2.47 -0.88 2.02 -3.09 7.85
Peroxisomes -0.96 1.03 2.26 2.00 1.20 1.40 0.27 7.06 1.76 7.71 3.13 0.84 7.74 2.48 8.35
Vacuole 8.95 7.76 8.02 -8.04 0.99 26.74* 22.90 26.66 -1.39 6.87 28.59* 23.86 26.05 -8.32 4.37
Vacuole Periphery 1.94 1.98 -1.66 -2.81 -2.83 -0.13 5.69 6.61 1.18 0.95 1.95 6.23 -0.24 -1.51 -2.51
Standard name
Human Ortholog
Description Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
Localization
Cell Percentages cell periphery (71%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Rsn1

Rsn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available