Standard name
Human Ortholog
Description Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate; human ortholog, PPA2, functionally complements the null mutant; mutations in human PPA2 cause a mitochondrial disease resulting in sudden unexpected cardiac arrest in infants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.06 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 – 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Cytoplasm 0.45 – 0.89 0.9 0.85 0.74 0.68 0.64 0.57 0.6 0.49 0.58 0.88 0.87 0.9 0.12 0.15 0.14 0.26 0.5 0.28 0.39 0.34 0.38
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.15 0.19 0 0 0.07 0 0.05 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.09 0.11 0 0 0 0 0 0
Mitochondria 0.61 – 0.07 0.05 0.06 0.23 0.33 0.3 0.5 0.41 0.56 0.41 0 0 0 0.14 0.14 0.13 0.46 0.33 0.43 0.35 0.33 0.28
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.16 0.16 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0.07 0.08 0.09 0.05 0.07 0.06 0.08 0 0 0 0.12 0.14 0.12 0.07 0 0.05 0 0.06 0.05
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.26 0.19 0.22 0.05 0.05 0 0.05 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 2 0 0 0 0 1 0 1 3 0 0 8 3 3 2 0 2 6 11 15
Bud 7 – 0 3 3 2 22 15 11 10 17 10 2 1 0 8 5 10 0 0 0 2 6 12
Bud Neck 0 – 2 9 20 9 15 25 9 12 4 9 7 2 0 0 0 0 2 0 1 2 6 12
Bud Site 0 – 0 0 0 0 2 3 4 4 2 2 2 0 0 0 0 2 – – – – – –
Cell Periphery 0 – 0 6 5 1 3 3 2 1 1 2 3 8 4 9 21 13 0 0 0 0 2 5
Cytoplasm 57 – 25 240 447 278 502 338 169 241 171 243 257 366 446 25 34 28 35 22 16 77 101 156
Endoplasmic Reticulum 1 – 0 1 0 0 0 0 0 1 0 0 2 2 1 15 13 8 2 0 0 0 1 6
Endosome 0 – 1 3 9 7 5 4 2 1 0 3 5 18 11 26 34 38 5 0 4 7 14 14
Golgi 0 – 0 0 0 1 0 0 0 0 0 0 1 0 3 30 20 22 0 0 0 0 2 3
Mitochondria 77 – 2 14 32 87 243 160 148 162 194 170 10 9 3 28 31 26 62 14 26 69 99 116
Nucleus 0 – 0 0 1 3 10 9 9 6 4 3 0 5 3 4 3 2 0 1 0 0 3 3
Nuclear Periphery 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 2
Nucleolus 0 – 0 1 1 0 2 2 0 1 4 0 0 0 2 1 2 2 0 0 0 1 1 1
Peroxisomes 0 – 0 1 0 2 3 4 1 1 0 2 3 9 10 19 35 32 1 0 0 4 5 3
SpindlePole 4 – 0 7 21 25 62 48 15 29 20 35 7 7 12 24 31 25 9 0 2 5 17 21
Vac/Vac Membrane 0 – 1 1 19 8 8 16 3 2 4 10 11 16 16 52 41 44 6 2 1 10 9 26
Unique Cell Count 126 28 268 524 374 739 530 296 400 347 418 291 419 494 202 221 202 136 45 61 197 302 417
Labelled Cell Count 146 31 286 560 423 877 627 373 473 422 490 313 443 511 250 274 255 136 45 61 197 302 417


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 – 5.3 4.6 4.9 4.1 3.5 4.1 3.5 3.6 3.3 3.6 5.0 5.4 5.7 6.6 6.9 6.8 5.0 6.8 6.0
Std Deviation (1e-4) 0.5 – 2.1 1.6 2.0 2.5 1.4 1.5 1.7 0.9 1.5 1.1 1.2 1.7 4.9 1.4 1.8 1.9 2.2 10.3 3.2
Intensity Change (Log2) – – – -0.21 -0.11 -0.37 -0.6 -0.36 -0.59 -0.58 -0.68 -0.56 -0.08 0.04 0.1 0.32 0.37 0.35 -0.09 0.35 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 -1.8 -2.4 -2.8 -3.3 -3.1 -4.1 -3.3 -0.2 -0.3 0.2 -9.2 -8.7 -8.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7741 -0.0367 -0.5378 -0.2496 -0.3701 0.2535 -0.425 0.2108 0.0145 -0.051 0.2097 0.1759 -0.9722 -0.5766 -0.7197 -0.6414 -0.6056 -0.9698
Actin 0.0395 0.016 0.003 0.0033 0.0026 0.019 0.0192 0.0047 0.0175 0.0103 0.0036 0.0096 0.0157 0.0159 0.0262 0.0045 0.0146 0.0133
Bud 0.0013 0.0047 0.0014 0.0047 0.0008 0.0013 0.0013 0.0027 0.0019 0.0014 0.0036 0.0005 0.0012 0.0036 0.0029 0.0022 0.006 0.0037
Bud Neck 0.0029 0.0017 0.0074 0.0003 0.0015 0.0035 0.0074 0.0014 0.0023 0.0005 0.0006 0.0096 0.0008 0.0014 0.0121 0.0026 0.0033 0.0032
Bud Periphery 0.0038 0.0098 0.0034 0.0108 0.0049 0.0015 0.0083 0.0055 0.0044 0.0035 0.0127 0.0017 0.005 0.0095 0.0075 0.0064 0.0082 0.0119
Bud Site 0.0042 0.0248 0.0164 0.0007 0.0038 0.001 0.0075 0.0235 0.0097 0.0011 0.0013 0.0006 0.0055 0.0199 0.0176 0.0034 0.0158 0.0014
Cell Periphery 0.0023 0.0021 0.0015 0.0004 0.0011 0.0007 0.0009 0.0029 0.0019 0.0006 0.0004 0.0006 0.0009 0.0023 0.0015 0.0032 0.001 0.0008
Cytoplasm 0.0452 0.0747 0.0552 0.0226 0.0198 0.0968 0.0657 0.0882 0.0989 0.0661 0.1348 0.1056 0.047 0.0793 0.0895 0.0676 0.1236 0.0825
Cytoplasmic Foci 0.0777 0.0781 0.1021 0.0375 0.0776 0.0872 0.0695 0.0786 0.0691 0.0675 0.0662 0.101 0.0933 0.0733 0.08 0.1087 0.0601 0.1304
Eisosomes 0.001 0.001 0.0007 0.0004 0.0007 0.0005 0.0011 0.0011 0.001 0.0013 0.0003 0.0003 0.0011 0.0017 0.0007 0.0025 0.0008 0.0004
Endoplasmic Reticulum 0.0327 0.0213 0.0022 0.0028 0.0021 0.0262 0.0044 0.0096 0.005 0.0056 0.003 0.0031 0.0079 0.0059 0.0052 0.0046 0.0072 0.0034
Endosome 0.0495 0.0674 0.0406 0.0512 0.0674 0.1108 0.076 0.0831 0.0573 0.102 0.0745 0.0814 0.107 0.0819 0.0742 0.0566 0.1142 0.0895
Golgi 0.0212 0.0211 0.0141 0.0129 0.0216 0.0371 0.0226 0.0228 0.0211 0.0276 0.0125 0.018 0.0311 0.024 0.0338 0.0175 0.0282 0.0581
Lipid Particles 0.0466 0.0208 0.0178 0.0689 0.0715 0.0374 0.0626 0.0171 0.0076 0.0113 0.0109 0.014 0.0506 0.0214 0.0232 0.0285 0.0129 0.0342
Mitochondria 0.1544 0.1552 0.2667 0.3658 0.1888 0.1684 0.2017 0.1845 0.1895 0.2127 0.2899 0.2791 0.276 0.1679 0.2935 0.2638 0.4884 0.3496
None 0.4183 0.4321 0.4169 0.3814 0.3877 0.3299 0.3889 0.4155 0.4584 0.4411 0.3462 0.3264 0.2858 0.4221 0.2731 0.3612 0.0563 0.1648
Nuclear Periphery 0.0242 0.0033 0.0008 0.0036 0.0011 0.024 0.0025 0.0021 0.0021 0.0017 0.001 0.0011 0.0054 0.0052 0.0038 0.0016 0.0151 0.0011
Nucleolus 0.0021 0.002 0.0015 0.0009 0.0014 0.0009 0.0046 0.0034 0.0007 0.0004 0.0006 0.0003 0.0007 0.0014 0.0011 0.0011 0.0011 0.0006
Nucleus 0.0222 0.0026 0.0013 0.0018 0.0009 0.0045 0.0026 0.0042 0.0015 0.0011 0.0011 0.0009 0.001 0.0028 0.0024 0.0014 0.0033 0.001
Peroxisomes 0.0296 0.0433 0.0317 0.0154 0.1323 0.0331 0.0341 0.0267 0.0395 0.0354 0.0252 0.0332 0.0478 0.0327 0.0328 0.053 0.0122 0.0396
Punctate Nuclear 0.0076 0.0035 0.0018 0.0032 0.0008 0.0048 0.0056 0.0041 0.0013 0.0011 0.0014 0.0012 0.0028 0.0011 0.008 0.0012 0.0025 0.0016
Vacuole 0.0102 0.0117 0.0109 0.0069 0.0077 0.0071 0.0104 0.0143 0.0066 0.0051 0.0076 0.0078 0.0095 0.0225 0.0075 0.0059 0.0179 0.0051
Vacuole Periphery 0.0034 0.003 0.0024 0.0047 0.0036 0.0044 0.0031 0.0038 0.0029 0.0023 0.0025 0.0038 0.0041 0.0042 0.0034 0.0025 0.0072 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.9747 16.9153 17.2589 25.9781 24.5335 21.2758 34.2062 28.4979 32.7641 29.6771
Translational Efficiency 0.7986 1.5 0.9594 0.8297 0.843 1.312 0.793 0.9762 1.1026 1.0175

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate; human ortholog, PPA2, functionally complements the null mutant; mutations in human PPA2 cause a mitochondrial disease resulting in sudden unexpected cardiac arrest in infants
Localization
Cell Percentages mitochondrion (19%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Ppa2

Ppa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ppa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available