Standard name
Human Ortholog
Description Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.99 1.0 0.98 0.94 0.92 – 0.82 0.79 0.76 0.72 0.99 0.99 1.0 0.98 0.96 0.93 0.85 0.93 0.89 0.89 0.87 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.08 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.13 0.07 – 0.21 0.24 0.32 0.38 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.06 0.06 0.05 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 1 0 1 0 0 0 0 0 0 2 0 2 0 2 2
Bud 0 0 0 0 0 3 0 – 5 8 9 10 0 0 0 0 0 0 0 0 0 2 4 7
Bud Neck 0 1 4 2 10 3 4 – 0 2 5 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 3 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 5 3 8 2 4 – 1 3 1 3 0 2 4 4 2 6 0 0 0 1 1 2
Cytoplasm 174 189 206 256 370 315 538 – 305 369 342 409 224 335 425 100 108 112 161 182 170 187 224 232
Endoplasmic Reticulum 3 0 2 1 1 0 1 – 0 1 0 2 1 0 0 6 9 7 0 0 0 1 3 2
Endosome 0 0 1 0 0 0 2 – 1 2 0 1 0 1 0 0 0 1 4 2 0 0 0 3
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 11 3 1 2 0 45 44 – 77 114 144 215 0 1 1 2 1 4 9 5 7 8 8 7
Nucleus 0 0 0 1 1 2 2 – 3 7 7 10 0 1 0 2 1 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 – 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 – 1 2 3 5 0 0 0 0 0 0 1 1 1 1 3 3
Vac/Vac Membrane 1 1 0 0 2 3 26 – 21 29 22 32 0 0 1 3 6 1 2 1 2 1 3 0
Unique Cell Count 182 191 209 257 376 334 587 372 468 449 572 226 337 426 102 113 120 189 196 192 210 258 265
Labelled Cell Count 190 196 219 265 394 373 624 414 539 537 692 226 340 431 117 127 134 189 196 192 210 258 265


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.3 4.7 4.6 5.4 4.0 4.7 – 4.3 4.4 4.2 4.4 4.9 5.1 5.1 6.8 6.8 6.5 4.5 5.0 5.1
Std Deviation (1e-4) 1.4 0.7 0.9 1.5 1.2 1.4 1.4 – 1.5 1.1 1.1 1.2 1.3 1.4 1.1 1.7 2.2 1.6 2.0 3.5 3.6
Intensity Change (Log2) – – – -0.01 0.2 -0.24 -0.01 – -0.11 -0.1 -0.14 -0.09 0.07 0.12 0.12 0.54 0.55 0.48 -0.04 0.1 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4691 0.4832 -0.457 -0.9811 -0.6934 -0.5582 0.775 1.6756 1.2719 0.9842 0.5498 1.4337 1.6014 3.1346 2.085 2.1747 2.5061 2.3987
Actin 0.021 0.0281 0.0049 0.004 0.0407 0.0036 0.0398 0.0027 0.0076 0.0072 0.0017 0.0103 0.0024 0.0002 0.0007 0.0034 0.0009 0.0009
Bud 0.0023 0.0007 0.0052 0.0055 0.0024 0.0004 0.0093 0.0135 0.003 0.0297 0.004 0.002 0.0003 0.0001 0.0001 0.0011 0 0.0001
Bud Neck 0.0044 0.0005 0.0008 0.0004 0.0009 0.0008 0.0047 0.0003 0.0005 0.0005 0.0005 0.0074 0.0003 0.001 0.0001 0.0002 0.0002 0.0004
Bud Periphery 0.0014 0.001 0.0055 0.0221 0.0019 0.0005 0.0034 0.006 0.0025 0.0218 0.0039 0.0018 0.0003 0.0001 0.0001 0.0035 0 0.0001
Bud Site 0.0111 0.0095 0.0046 0.0006 0.0031 0.0002 0.0051 0.015 0.0089 0.0033 0.0098 0.0007 0.0029 0.0032 0.0008 0.0003 0.0001 0
Cell Periphery 0.0004 0.0005 0.0003 0.0002 0.0001 0 0.0009 0.0008 0.0001 0.0003 0.001 0.0001 0.0002 0.0001 0.0001 0.0001 0 0
Cytoplasm 0.3329 0.4647 0.411 0.3097 0.4552 0.4837 0.3223 0.6181 0.4695 0.4702 0.4635 0.5889 0.3068 0.4252 0.4048 0.4593 0.391 0.4634
Cytoplasmic Foci 0.0497 0.0091 0.0185 0.0113 0.0349 0.0187 0.0336 0.0084 0.0154 0.012 0.0181 0.011 0.0109 0.0038 0.007 0.0078 0.0045 0.0054
Eisosomes 0.0002 0.0005 0.0003 0.0001 0.0001 0.0001 0.0026 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0045 0.003 0.0038 0.0031 0.0013 0.002 0.003 0.0018 0.0023 0.0012 0.0015 0.0021 0.0017 0.0009 0.0018 0.0071 0.0014 0.001
Endosome 0.0401 0.0054 0.0112 0.0083 0.0139 0.0063 0.0201 0.0018 0.0119 0.0101 0.008 0.0113 0.0021 0.0015 0.0012 0.0056 0.002 0.0008
Golgi 0.007 0.0027 0.0028 0.0027 0.005 0.0008 0.009 0.0003 0.0088 0.0049 0.0074 0.0021 0.0007 0.0001 0.0002 0.0005 0.0001 0.0001
Lipid Particles 0.0189 0.0007 0.0067 0.0004 0.001 0.0025 0.0212 0.0001 0.0029 0.0183 0.0098 0.0022 0.0016 0.0001 0.0001 0.0004 0.0001 0
Mitochondria 0.0065 0.0157 0.012 0.0196 0.0012 0.0033 0.0149 0.0013 0.0146 0.0017 0.0095 0.0007 0.0005 0.0001 0.0002 0.0007 0.0002 0.0001
None 0.4592 0.4484 0.4922 0.6 0.4293 0.4608 0.4756 0.3195 0.4357 0.4041 0.4418 0.3473 0.6615 0.5613 0.5802 0.496 0.584 0.5248
Nuclear Periphery 0.0053 0.0012 0.0024 0.0018 0.0007 0.0045 0.0045 0.0006 0.0013 0.001 0.0009 0.0024 0.0005 0.0003 0.0004 0.0036 0.003 0.0003
Nucleolus 0.0045 0.0002 0.0009 0.0003 0.0001 0.0002 0.0007 0.0009 0.0002 0.0003 0.0025 0.0002 0.0001 0.0001 0.0001 0.0002 0.0005 0
Nucleus 0.0043 0.0017 0.0047 0.0029 0.0013 0.0046 0.0028 0.0039 0.0021 0.002 0.0035 0.0029 0.0009 0.0008 0.0009 0.0038 0.0073 0.0009
Peroxisomes 0.0133 0.002 0.006 0.0004 0.0031 0.0019 0.0206 0.0007 0.0088 0.0077 0.0069 0.0018 0.0039 0 0.0003 0.0003 0.0001 0.0004
Punctate Nuclear 0.0075 0.0007 0.0021 0.0027 0.0022 0.004 0.0022 0.0031 0.002 0.002 0.0013 0.0034 0.0014 0.0004 0.0006 0.003 0.0038 0.0005
Vacuole 0.0043 0.0036 0.0032 0.0025 0.0013 0.0009 0.0029 0.0011 0.0015 0.0011 0.0036 0.001 0.0006 0.0006 0.0003 0.0022 0.0005 0.0005
Vacuole Periphery 0.0012 0.0002 0.001 0.0013 0.0003 0.0004 0.001 0.0001 0.0005 0.0003 0.0007 0.0003 0.0001 0.0001 0 0.0005 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4527 2.8841 7.4905 4.8551 3.1373 6.4491 9.113 8.8569 11.3013 8.0618
Translational Efficiency 0.6123 0.822 0.3686 0.6826 1.1042 1.2488 0.7817 0.6425 0.6211 0.5674

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2406 1221 1782 1824 1927 1012 1760 1672 4333 2233 3542 3496

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 633.99 751.96 917.08 850.56 658.96 736.78 877.14 855.87 645.09 745.08 897.23 853.10
Standard Deviation 187.96 102.99 167.59 145.22 74.59 95.75 119.31 111.82 149.15 100.06 146.98 130.35
Intensity Change Log 2 — 0.246196 0.532588 0.423953 — 0.161043 0.412616 0.377201 — 0.203425 0.472690 0.400315

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000207 0.000491 0.000176 0.001007 0.000076 0.000196 0.000188 0.001014 0.000149 0.000357 0.000182 0.001010
Bud Neck 0.031466 0.018385 0.003442 0.007777 0.012382 0.030534 0.001930 0.005400 0.022979 0.023891 0.002690 0.006640
Bud Site 0.012159 0.014812 0.008867 0.031006 0.004075 0.024158 0.004138 0.029225 0.008564 0.019048 0.006517 0.030154
Cell Periphery 0.000294 0.000164 0.000094 0.000169 0.000104 0.000177 0.000098 0.000160 0.000209 0.000170 0.000096 0.000165
Cytoplasm 0.424918* 0.500971* 0.611363* 0.521466* 0.530216* 0.530165* 0.488191* 0.576605* 0.471747* 0.514201* 0.550160* 0.547837*
Cytoplasmic Foci 0.280366* 0.252635* 0.011357 0.020333 0.189529* 0.166432 0.010491 0.019273 0.239969* 0.213568* 0.010927 0.019826
Eisosomes 0.000400 0.000138 0.000018 0.000041 0.000120 0.000104 0.000026 0.000043 0.000276 0.000122 0.000022 0.000042
Endoplasmic Reticulum 0.001765 0.001242 0.001475 0.001124 0.000624 0.000377 0.001613 0.000936 0.001258 0.000850 0.001544 0.001034
Endosome 0.010318 0.018902 0.001560 0.009930 0.007688 0.012172 0.001385 0.003562 0.009149 0.015852 0.001473 0.006885
Golgi 0.009718 0.006174 0.000103 0.004089 0.001711 0.002798 0.000054 0.000954 0.006157 0.004644 0.000078 0.002590
Lipid Particles 0.014321 0.006069 0.000629 0.000996 0.007031 0.003485 0.000785 0.000816 0.011079 0.004898 0.000707 0.000910
Mitochondria 0.019793 0.006483 0.000576 0.005998 0.005972 0.006493 0.000471 0.001357 0.013647 0.006487 0.000524 0.003778
Mitotic Spindle 0.002263 0.002094 0.006804 0.070356 0.003265 0.006036 0.003367 0.013537 0.002709 0.003881 0.005096 0.043181
None 0.006238 0.002503 0.006352 0.005213 0.002594 0.006341 0.005764 0.004148 0.004617 0.004242 0.006060 0.004704
Nuclear Periphery 0.000304 0.000368 0.000982 0.001582 0.000395 0.000226 0.001235 0.000877 0.000345 0.000304 0.001108 0.001245
Nuclear Periphery Foci 0.000288 0.000971 0.001508 0.001978 0.000370 0.000178 0.001270 0.000551 0.000324 0.000612 0.001390 0.001295
Nucleolus 0.001652 0.001585 0.000249 0.001550 0.001172 0.001767 0.000255 0.000439 0.001439 0.001668 0.000252 0.001019
Nucleus 0.141980* 0.088964 0.299516* 0.199861* 0.193374* 0.148983* 0.432048* 0.229701* 0.164836* 0.116164 0.365370* 0.214133*
Peroxisomes 0.008048 0.009104 0.000182 0.001201 0.003251 0.007086 0.000123 0.000818 0.005915 0.008190 0.000153 0.001018
Vacuole 0.031349 0.065860 0.044605 0.108800 0.035419 0.051151 0.046460 0.109975 0.033159 0.059194 0.045527 0.109362
Vacuole Periphery 0.002153 0.002085 0.000143 0.005523 0.000631 0.001144 0.000106 0.000608 0.001476 0.001658 0.000125 0.003173

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.95 -0.20 -2.50 -0.69 -2.46 -4.73 -3.49 -1.97 -1.52 -1.40 -3.87 -2.78 -3.00 -1.57 -2.53
Bud Neck 6.74 19.00 16.95 8.27 -3.37 -6.89 9.32 6.56 10.40 -5.03 -0.45 20.99 17.79 13.25 -5.72
Bud Site -1.54 2.63 -3.07 -1.70 -4.99 -6.74 -0.07 -6.61 1.64 -6.38 -6.38 2.63 -6.55 0.33 -8.09
Cell Periphery 2.92 4.49 4.19 3.49 -1.14 -2.89 0.13 -1.68 2.06 -1.71 1.45 4.46 3.76 3.64 -2.02
Cytoplasm -7.68 -17.32 -3.63 4.08 11.86 0.06 12.50 5.88 4.67 -5.64 -5.71 -3.79 0.96 6.09 4.33
Cytoplasmic Foci 3.57 58.10 57.12 37.22 -2.68 3.06 39.99 38.13 24.73 -6.55 4.74 68.96 67.04 43.66 -6.40
Eisosomes 11.20 18.82 17.67 7.57 -12.98 1.97 16.43 13.39 7.40 -6.57 11.22 21.29 19.65 10.00 -13.15
Endoplasmic Reticulum 1.79 -0.89 1.06 -1.43 3.75 2.79 -14.81 -8.99 -14.37 7.84 2.44 -6.13 -1.70 -6.36 7.96
Endosome -6.10 15.64 6.69 9.77 -4.78 -3.22 10.31 7.64 7.39 -2.28 -6.72 18.56 10.09 12.32 -5.28
Golgi 3.93 16.07 11.52 5.63 -4.01 -2.04 7.32 4.02 4.36 -1.86 2.72 17.26 12.29 7.13 -4.41
Lipid Particles 8.64 18.14 17.79 8.31 -4.21 5.40 11.65 11.64 5.86 -0.34 10.19 21.36 21.07 10.10 -3.13
Mitochondria 8.78 15.11 11.76 3.24 -3.35 -0.33 4.94 4.53 4.68 -1.85 6.44 15.12 12.48 5.66 -3.73
Mitotic Spindle 0.29 -3.53 -10.68 -10.76 -8.82 -1.48 -0.07 -3.78 -1.78 -3.80 -1.20 -2.80 -11.23 -10.24 -9.59
None 4.86 0.64 1.61 -5.47 1.84 -2.98 -6.07 -2.91 2.07 2.91 0.57 -1.48 0.53 -0.25 3.19
Nuclear Periphery -0.91 -21.44 -13.28 -11.68 -6.33 4.37 -22.59 -18.60 -22.11 2.15 0.92 -31.26 -19.22 -18.07 -4.74
Nuclear Periphery Foci -3.38 -9.65 -2.91 -0.84 1.87 1.39 -7.46 -3.33 -11.94 6.57 -2.27 -12.40 -3.95 -2.26 3.78
Nucleolus 0.54 4.73 2.40 2.03 -2.79 -1.20 4.33 3.78 3.15 -1.73 -0.48 6.25 3.93 3.77 -3.13
Nucleus 11.10 -26.71 -15.40 -23.45 10.48 6.16 -33.88 -7.88 -12.09 23.92 11.44 -42.64 -16.76 -24.68 24.27
Peroxisomes -1.13 17.99 16.31 10.04 -2.66 -4.69 9.53 7.84 8.74 -2.19 -3.58 20.17 17.87 13.27 -3.45
Vacuole -13.16 -12.59 -30.03 -21.79 -20.23 -5.42 -13.84 -27.73 -23.69 -16.36 -13.43 -18.78 -40.89 -32.03 -25.68
Vacuole Periphery 0.38 12.85 -0.12 -0.31 -4.29 -3.69 8.04 2.28 4.87 -2.12 -1.03 14.65 0.57 1.11 -4.63
Standard name
Human Ortholog
Description Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (39%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ura10

Ura10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available