Standard name | |
---|---|
Human Ortholog | |
Description | Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 7 | 10 | 4 | 3 | 2 | 2 | 3 | 9 | 2 | 1 | 13 | 7 | 3 | 3 | 1 | 6 | 1 | 1 | 5 | 1 | 7 | 3 |
Bud | 2 | 1 | 11 | 14 | 10 | 11 | 36 | 20 | 33 | 28 | 31 | 20 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 5 | 3 | 5 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 3 | 8 | 10 | 4 | 6 | 7 | 12 | 15 | 4 | 6 | 12 | 10 | 15 | 4 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 175 | 111 | 174 | 206 | 308 | 288 | 422 | 472 | 361 | 426 | 248 | 282 | 334 | 253 | 359 | 238 | 210 | 167 | 32 | 34 | 22 | 83 | 115 | 143 |
Endoplasmic Reticulum | 48 | 16 | 53 | 43 | 29 | 9 | 8 | 13 | 12 | 18 | 20 | 23 | 194 | 108 | 101 | 4 | 4 | 3 | 150 | 64 | 184 | 124 | 167 | 191 |
Endosome | 0 | 0 | 5 | 5 | 4 | 0 | 2 | 10 | 3 | 1 | 1 | 2 | 3 | 7 | 8 | 4 | 2 | 4 | 4 | 1 | 3 | 4 | 8 | 5 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 3 | 0 | 2 | 0 |
Mitochondria | 2 | 3 | 1 | 5 | 0 | 8 | 16 | 15 | 12 | 44 | 36 | 26 | 0 | 0 | 0 | 2 | 6 | 3 | 5 | 0 | 4 | 5 | 9 | 6 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 1 | 2 | 1 | 0 | 1 | 1 | 3 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 4 | 0 | 3 | 2 | 3 | 7 | 8 | 18 | 16 | 17 | 17 | 20 | 8 | 16 | 2 | 24 | 9 | 13 | 57 | 29 | 32 | 69 | 60 | 87 |
Unique Cell Count | 228 | 131 | 235 | 260 | 338 | 306 | 454 | 514 | 396 | 488 | 323 | 346 | 519 | 375 | 463 | 266 | 225 | 189 | 266 | 141 | 266 | 301 | 392 | 461 |
Labelled Cell Count | 235 | 134 | 258 | 293 | 368 | 335 | 504 | 563 | 453 | 561 | 363 | 382 | 566 | 406 | 489 | 283 | 244 | 201 | 266 | 141 | 266 | 301 | 392 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 6.9 | 7.2 | 7.3 | 7.4 | 6.0 | 5.6 | 5.7 | 5.6 | 5.2 | 5.3 | 5.4 | 9.5 | 8.7 | 7.7 | 9.7 | 9.4 | 9.1 | 9.1 | 9.9 | 9.7 |
Std Deviation (1e-4) | 1.0 | 1.5 | 1.3 | 1.8 | 1.5 | 1.7 | 1.2 | 1.3 | 1.5 | 1.1 | 1.1 | 1.2 | 1.8 | 1.6 | 1.3 | 2.2 | 2.1 | 2.0 | 1.8 | 2.1 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.04 | -0.25 | -0.35 | -0.33 | -0.36 | -0.46 | -0.43 | -0.41 | 0.4 | 0.28 | 0.11 | 0.44 | 0.4 | 0.34 | 0.34 | 0.47 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | 0 | 0 | 0 | 0 | 0 | -1.0 | 0 | 0 | -0.4 | -0.9 | 0 | 0 | 0 | 0.1 |
Bud | 0.4 | -1.1 | -0.6 | 1.6 | -0.5 | 1.7 | 0.6 | 2.2 | 0.6 | 0 | 0 | 0 | -3.6 | -2.5 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.4 | 1.3 | 0 | 0 | 0 | 1.4 | 1.5 | 0 | 0 | 0 | 1.2 | 1.5 | 0 | 0 | 0 |
Cytoplasm | 1.4 | 5.5 | 6.6 | 6.9 | 6.6 | 5.8 | 4.4 | 0.7 | 2.1 | -2.6 | -1.7 | 1.0 | 4.5 | 5.6 | 3.7 |
Endoplasmic Reticulum | -1.7 | -4.7 | -7.1 | -9.1 | -9.0 | -7.8 | -8.0 | -5.7 | -5.6 | 4.0 | 1.7 | -0.2 | -7.4 | -6.8 | -6.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.5 | 2.2 | 0 | 4.5 | 5.0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.7 | 2.0 | 1.7 | 2.5 | 2.7 | 0 | 2.1 | 0 | 3.8 | 1.8 | 3.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.9579 | 10.848 | 11.1811 | 10.5947 | 9.3365 | 10.3511 | 8.119 | 10.3754 | 8.5869 | 8.6036 | 7.9471 | 9.2667 | 7.5398 | 9.0682 | 8.2581 | 7.9347 | 6.3484 | 7.6545 |
Actin | 0.0055 | 0.0009 | 0.0037 | 0.0006 | 0.0006 | 0.0004 | 0.0105 | 0.0006 | 0.0096 | 0.0383 | 0.0127 | 0.0006 | 0.0149 | 0.0021 | 0.0086 | 0.0011 | 0.0066 | 0.0024 |
Bud | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0011 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0015 | 0.0002 |
Bud Neck | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.001 | 0.0038 | 0.0012 | 0.0007 | 0.0008 | 0.0008 | 0.0232 | 0.0018 | 0.0028 | 0.0013 | 0.0005 | 0.002 | 0.0005 | 0.0018 |
Bud Periphery | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0018 | 0.0004 | 0.0004 | 0.0014 | 0.0009 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0002 |
Bud Site | 0.0002 | 0.0024 | 0.0017 | 0.0001 | 0.0003 | 0.0001 | 0.0065 | 0.0023 | 0.0007 | 0.0012 | 0.0168 | 0.0001 | 0.0048 | 0.0005 | 0.0018 | 0.0041 | 0.0092 | 0.0004 |
Cell Periphery | 0.0007 | 0.0014 | 0.0011 | 0.0007 | 0.0004 | 0.0003 | 0.0011 | 0.0035 | 0.0008 | 0.0122 | 0.0004 | 0.0005 | 0.0009 | 0.0016 | 0.0014 | 0.0008 | 0.0005 | 0.0004 |
Cytoplasm | 0.1457 | 0.2534 | 0.1488 | 0.0781 | 0.2943 | 0.1963 | 0.1489 | 0.215 | 0.2073 | 0.1567 | 0.2154 | 0.1763 | 0.1173 | 0.2283 | 0.1064 | 0.1067 | 0.2505 | 0.1776 |
Cytoplasmic Foci | 0.0098 | 0.0131 | 0.0126 | 0.0057 | 0.0208 | 0.0118 | 0.0185 | 0.0097 | 0.0111 | 0.0103 | 0.0093 | 0.0046 | 0.0138 | 0.0052 | 0.0221 | 0.0141 | 0.0086 | 0.0144 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.7056 | 0.59 | 0.6896 | 0.7833 | 0.4571 | 0.6477 | 0.5637 | 0.5198 | 0.4768 | 0.3486 | 0.5328 | 0.5337 | 0.692 | 0.6272 | 0.6437 | 0.5177 | 0.4241 | 0.5774 |
Endosome | 0.0554 | 0.0478 | 0.0643 | 0.0248 | 0.1002 | 0.072 | 0.0731 | 0.0495 | 0.0909 | 0.1058 | 0.0347 | 0.0781 | 0.0568 | 0.0396 | 0.0748 | 0.1668 | 0.0691 | 0.0853 |
Golgi | 0.0029 | 0.0062 | 0.006 | 0.004 | 0.005 | 0.0042 | 0.0089 | 0.0039 | 0.0142 | 0.0165 | 0.0238 | 0.0013 | 0.0079 | 0.0034 | 0.0086 | 0.0101 | 0.0036 | 0.0045 |
Lipid Particles | 0.0159 | 0.0371 | 0.0124 | 0.0269 | 0.016 | 0.0067 | 0.0069 | 0.0119 | 0.0129 | 0.0226 | 0.0057 | 0.004 | 0.0118 | 0.0087 | 0.0144 | 0.0109 | 0.0061 | 0.02 |
Mitochondria | 0.0006 | 0.0004 | 0.0007 | 0.0002 | 0.0009 | 0.0005 | 0.0075 | 0.0008 | 0.0123 | 0.0078 | 0.0055 | 0.0005 | 0.0026 | 0.0004 | 0.0023 | 0.0133 | 0.0439 | 0.0095 |
None | 0.0089 | 0.0017 | 0.0037 | 0.0004 | 0.0036 | 0.0027 | 0.0037 | 0.0007 | 0.001 | 0.0014 | 0.0005 | 0.0003 | 0.0037 | 0.0005 | 0.0011 | 0.0004 | 0.0014 | 0.0008 |
Nuclear Periphery | 0.0234 | 0.0139 | 0.0136 | 0.0119 | 0.0242 | 0.0158 | 0.0663 | 0.0412 | 0.0435 | 0.0528 | 0.0664 | 0.0477 | 0.0348 | 0.0216 | 0.0243 | 0.0314 | 0.0617 | 0.0292 |
Nucleolus | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0019 | 0 | 0.0002 | 0 | 0.0001 | 0.0008 | 0.0005 | 0.0002 |
Nucleus | 0.0026 | 0.0032 | 0.003 | 0.0012 | 0.0082 | 0.0029 | 0.0069 | 0.0051 | 0.0041 | 0.0047 | 0.0076 | 0.0065 | 0.0031 | 0.0023 | 0.002 | 0.0021 | 0.0143 | 0.0038 |
Peroxisomes | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0018 | 0.0004 | 0.0011 | 0.0067 | 0.0018 | 0 | 0.0029 | 0.0001 | 0.0056 | 0.0042 | 0.001 | 0.0042 |
Punctate Nuclear | 0.0007 | 0.0007 | 0.0018 | 0.0002 | 0.0013 | 0.0005 | 0.0041 | 0.0007 | 0.0015 | 0.0016 | 0.0059 | 0.0004 | 0.0019 | 0.0003 | 0.0013 | 0.0003 | 0.0024 | 0.0019 |
Vacuole | 0.0191 | 0.0253 | 0.0334 | 0.0581 | 0.0612 | 0.0313 | 0.0574 | 0.1277 | 0.098 | 0.1938 | 0.0304 | 0.1336 | 0.0242 | 0.053 | 0.0746 | 0.1024 | 0.0846 | 0.0598 |
Vacuole Periphery | 0.0022 | 0.0013 | 0.0024 | 0.003 | 0.004 | 0.0025 | 0.0091 | 0.0055 | 0.0123 | 0.0159 | 0.0037 | 0.0097 | 0.0027 | 0.0034 | 0.0059 | 0.0101 | 0.0079 | 0.0061 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.6771 | 32.4522 | 32.3191 | 30.6405 | 30.9656 | 42.0112 | 35.4028 | 25.3515 | 35.1367 | 41.851 |
Translational Efficiency | 1.2081 | 1.116 | 0.9039 | 1.3648 | 1.8069 | 1.6696 | 1.0565 | 1.4401 | 1.3409 | 1.173 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1847 | 810 | 1907 | 1539 | 1697 | 1721 | 639 | 210 | 3544 | 2531 | 2546 | 1749 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 905.30 | 941.47 | 1300.57 | 1223.52 | 913.67 | 1024.67 | 1338.77 | 1485.73 | 909.31 | 998.04 | 1310.16 | 1255.00 |
Standard Deviation | 127.58 | 172.79 | 194.31 | 193.73 | 136.75 | 152.18 | 213.27 | 250.97 | 132.12 | 163.73 | 199.93 | 218.75 |
Intensity Change Log 2 | — | 0.056519 | 0.522676 | 0.434570 | — | 0.165414 | 0.551163 | 0.701427 | — | 0.112244 | 0.537055 | 0.574772 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000527 | 0.001278 | 0.001455 | 0.008206 | 0.000705 | 0.000674 | 0.002712 | 0.005385 | 0.000612 | 0.000867 | 0.001770 | 0.007867 |
Bud Neck | 0.004307 | 0.003817 | 0.002047 | 0.006021 | 0.002938 | 0.003010 | 0.003062 | 0.007507 | 0.003652 | 0.003268 | 0.002302 | 0.006200 |
Bud Site | 0.005529 | 0.011922 | 0.007261 | 0.041993 | 0.005269 | 0.008422 | 0.015698 | 0.030539 | 0.005404 | 0.009542 | 0.009379 | 0.040618 |
Cell Periphery | 0.001065 | 0.000745 | 0.001347 | 0.013441 | 0.001037 | 0.001272 | 0.002179 | 0.016583 | 0.001052 | 0.001104 | 0.001556 | 0.013818 |
Cytoplasm | 0.253562* | 0.172010* | 0.104818* | 0.152118* | 0.225833* | 0.140747* | 0.081513 | 0.068558 | 0.240284* | 0.150753* | 0.098969 | 0.142085* |
Cytoplasmic Foci | 0.073846 | 0.120755 | 0.011940 | 0.033423 | 0.074558 | 0.042585 | 0.020862 | 0.033473 | 0.074187 | 0.067602 | 0.014179 | 0.033429 |
Eisosomes | 0.000686 | 0.000147 | 0.000083 | 0.000580 | 0.000521 | 0.000346 | 0.000135 | 0.000434 | 0.000607 | 0.000282 | 0.000096 | 0.000562 |
Endoplasmic Reticulum | 0.489249* | 0.381467* | 0.734720* | 0.497150* | 0.533344* | 0.596974* | 0.694306* | 0.428638* | 0.510363* | 0.528005* | 0.724577* | 0.488924* |
Endosome | 0.019188 | 0.061115 | 0.022325 | 0.027981 | 0.019090 | 0.025982 | 0.023254 | 0.046359 | 0.019141 | 0.037226 | 0.022558 | 0.030188 |
Golgi | 0.015348 | 0.014776 | 0.006269 | 0.017269 | 0.016978 | 0.010958 | 0.004045 | 0.019094 | 0.016129 | 0.012179 | 0.005710 | 0.017488 |
Lipid Particles | 0.032500 | 0.038791 | 0.032881 | 0.099643 | 0.032007 | 0.020827 | 0.042523 | 0.217498* | 0.032264 | 0.026576 | 0.035301 | 0.113793* |
Mitochondria | 0.003370 | 0.002321 | 0.000649 | 0.001534 | 0.003012 | 0.002913 | 0.000261 | 0.000170 | 0.003198 | 0.002724 | 0.000552 | 0.001370 |
Mitotic Spindle | 0.000206 | 0.000832 | 0.001551 | 0.003269 | 0.000625 | 0.001124 | 0.003000 | 0.011728 | 0.000407 | 0.001030 | 0.001915 | 0.004285 |
None | 0.002829 | 0.000842 | 0.001601 | 0.000364 | 0.004279 | 0.000598 | 0.001513 | 0.000103 | 0.003523 | 0.000676 | 0.001579 | 0.000333 |
Nuclear Periphery | 0.001460 | 0.001813 | 0.001658 | 0.000366 | 0.001213 | 0.001155 | 0.001932 | 0.000628 | 0.001342 | 0.001365 | 0.001727 | 0.000398 |
Nuclear Periphery Foci | 0.003804 | 0.013235 | 0.034059 | 0.011481 | 0.005074 | 0.003811 | 0.039665 | 0.026509 | 0.004412 | 0.006827 | 0.035466 | 0.013286 |
Nucleolus | 0.000350 | 0.000564 | 0.000041 | 0.000076 | 0.000440 | 0.000210 | 0.000076 | 0.000073 | 0.000393 | 0.000324 | 0.000050 | 0.000076 |
Nucleus | 0.021026 | 0.006690 | 0.003715 | 0.003839 | 0.017378 | 0.006993 | 0.005078 | 0.002078 | 0.019279 | 0.006896 | 0.004057 | 0.003628 |
Peroxisomes | 0.001820 | 0.001946 | 0.000182 | 0.001670 | 0.001477 | 0.001081 | 0.000763 | 0.001338 | 0.001655 | 0.001358 | 0.000328 | 0.001630 |
Vacuole | 0.066412 | 0.154438 | 0.030440 | 0.075868 | 0.051284 | 0.123165 | 0.056718 | 0.081692 | 0.059168 | 0.133173 | 0.037035 | 0.076567 |
Vacuole Periphery | 0.002919 | 0.010496 | 0.000957 | 0.003706 | 0.002937 | 0.007153 | 0.000706 | 0.001615 | 0.002928 | 0.008223 | 0.000894 | 0.003455 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.43 | -3.24 | -10.01 | -8.51 | -8.28 | 0.20 | -3.22 | -4.96 | -5.02 | -2.30 | -1.97 | -4.36 | -10.61 | -10.18 | -8.29 |
Bud Neck | 0.63 | 3.34 | -2.58 | -3.59 | -7.81 | -0.15 | -0.30 | -4.73 | -4.66 | -4.17 | 0.79 | 2.86 | -5.06 | -6.11 | -8.34 |
Bud Site | -4.21 | -1.73 | -13.39 | -10.24 | -12.31 | -2.72 | -4.16 | -5.29 | -4.64 | -2.91 | -4.54 | -4.01 | -14.46 | -12.44 | -12.11 |
Cell Periphery | 2.94 | -2.10 | -12.11 | -12.43 | -11.75 | -1.54 | -2.02 | -6.10 | -6.00 | -5.50 | -0.52 | -2.88 | -13.42 | -13.36 | -12.69 |
Cytoplasm | 8.50 | 21.57 | 12.64 | 1.82 | -7.33 | 11.84 | 16.68 | 12.78 | 5.76 | 0.75 | 15.97 | 26.89 | 14.91 | 0.80 | -7.50 |
Cytoplasmic Foci | -7.76 | 21.78 | 12.27 | 15.18 | -10.55 | 8.99 | 14.33 | 6.63 | 1.38 | -2.33 | 2.13 | 27.53 | 15.26 | 11.62 | -9.94 |
Eisosomes | 3.72 | 4.17 | 0.75 | -8.45 | -10.17 | 1.76 | 5.45 | 1.15 | -0.75 | -4.36 | 3.38 | 6.21 | 0.58 | -4.12 | -10.58 |
Endoplasmic Reticulum | 7.07 | -21.30 | 0.63 | -6.29 | 20.50 | -5.01 | -8.60 | 4.67 | 6.95 | 9.18 | -1.69 | -22.96 | 3.48 | 4.65 | 21.79 |
Endosome | -13.86 | -2.83 | -5.90 | 10.39 | -3.30 | -4.99 | -2.34 | -5.34 | -3.96 | -4.26 | -13.16 | -3.74 | -7.95 | 3.86 | -4.73 |
Golgi | 0.41 | 9.19 | -0.85 | -1.05 | -6.91 | 4.62 | 10.78 | -0.37 | -2.20 | -4.33 | 4.17 | 13.26 | -0.55 | -3.24 | -8.15 |
Lipid Particles | -2.08 | -0.62 | -14.87 | -11.89 | -13.61 | 6.19 | -2.52 | -9.93 | -10.56 | -9.03 | 3.72 | -1.95 | -17.72 | -18.71 | -15.73 |
Mitochondria | 1.52 | 6.92 | 3.89 | 1.29 | -2.71 | 0.18 | 10.40 | 10.81 | 5.53 | 1.23 | 1.06 | 10.79 | 5.32 | 3.00 | -2.89 |
Mitotic Spindle | -2.69 | -3.88 | -3.93 | -3.12 | -1.81 | -0.77 | -2.08 | -2.63 | -2.54 | -2.04 | -1.51 | -3.85 | -4.50 | -3.68 | -2.43 |
None | 3.30 | 2.04 | 5.07 | 1.66 | 3.03 | 4.02 | 2.28 | 4.58 | 4.33 | 1.65 | 5.44 | 3.21 | 6.30 | 2.56 | 3.33 |
Nuclear Periphery | -0.97 | -1.00 | 5.71 | 4.51 | 7.42 | 0.29 | -1.92 | 2.29 | 1.76 | 3.02 | -0.12 | -2.23 | 7.79 | 5.83 | 8.16 |
Nuclear Periphery Foci | -6.22 | -16.06 | -10.38 | 0.80 | 11.15 | 2.83 | -11.00 | -5.44 | -5.74 | 2.51 | -4.33 | -19.18 | -11.00 | -7.01 | 12.20 |
Nucleolus | -0.80 | 3.58 | 3.13 | 2.08 | -3.54 | 1.81 | 2.69 | 2.87 | 5.14 | 0.29 | 0.67 | 4.56 | 4.19 | 3.09 | -2.10 |
Nucleus | 13.14 | 17.68 | 17.04 | 3.56 | -0.99 | 9.11 | 10.59 | 13.26 | 4.69 | 3.00 | 15.13 | 21.22 | 21.13 | 4.82 | 0.34 |
Peroxisomes | -0.30 | 7.97 | 0.85 | 1.10 | -7.97 | 2.31 | 1.78 | 0.54 | -1.01 | -1.28 | 1.93 | 8.46 | 0.41 | -1.21 | -6.79 |
Vacuole | -11.93 | 10.29 | -4.04 | 8.91 | -12.79 | -13.66 | -2.32 | -4.37 | 2.57 | -2.90 | -17.26 | 7.23 | -6.90 | 9.03 | -11.74 |
Vacuole Periphery | -9.04 | 6.77 | -1.01 | 6.68 | -4.02 | -5.14 | 8.02 | 3.22 | 6.49 | -2.20 | -8.55 | 9.95 | -0.74 | 5.89 | -4.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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