Standard name
Human Ortholog
Description Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0.05 0 0 0.08 0 0.08 0.06 0.1 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.85 0.74 0.79 0.91 0.94 0.93 0.92 0.91 0.87 0.77 0.82 0.64 0.67 0.78 0.89 0.93 0.88 0.12 0.25 0.09 0.28 0.29 0.31
Endoplasmic Reticulum 0.21 0.12 0.23 0.17 0.09 0 0 0 0 0 0.06 0.07 0.37 0.29 0.22 0 0 0 0.57 0.46 0.69 0.41 0.43 0.42
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.09 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.09 0 0.07 0.22 0.21 0.12 0.23 0.16 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 7 10 4 3 2 2 3 9 2 1 13 7 3 3 1 6 1 1 5 1 7 3
Bud 2 1 11 14 10 11 36 20 33 28 31 20 1 0 0 0 2 0 1 1 1 2 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 5 3 5 4
Bud Site 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0
Cell Periphery 0 1 3 8 10 4 6 7 12 15 4 6 12 10 15 4 6 4 0 0 0 0 0 1
Cytoplasm 175 111 174 206 308 288 422 472 361 426 248 282 334 253 359 238 210 167 32 34 22 83 115 143
Endoplasmic Reticulum 48 16 53 43 29 9 8 13 12 18 20 23 194 108 101 4 4 3 150 64 184 124 167 191
Endosome 0 0 5 5 4 0 2 10 3 1 1 2 3 7 8 4 2 4 4 1 3 4 8 5
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 3 0 2 0
Mitochondria 2 3 1 5 0 8 16 15 12 44 36 26 0 0 0 2 6 3 5 0 4 5 9 6
Nucleus 0 1 0 0 0 2 3 0 1 1 2 1 0 1 1 3 4 1 0 0 0 0 0 1
Nuclear Periphery 2 0 0 0 0 1 1 3 0 0 1 0 0 0 0 0 0 0 2 0 1 1 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 1 1 4 0 1 0 0 0 0 0 0 1 1
Vac/Vac Membrane 4 0 3 2 3 7 8 18 16 17 17 20 8 16 2 24 9 13 57 29 32 69 60 87
Unique Cell Count 228 131 235 260 338 306 454 514 396 488 323 346 519 375 463 266 225 189 266 141 266 301 392 461
Labelled Cell Count 235 134 258 293 368 335 504 563 453 561 363 382 566 406 489 283 244 201 266 141 266 301 392 461


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.9 7.2 7.3 7.4 6.0 5.6 5.7 5.6 5.2 5.3 5.4 9.5 8.7 7.7 9.7 9.4 9.1 9.1 9.9 9.7
Std Deviation (1e-4) 1.0 1.5 1.3 1.8 1.5 1.7 1.2 1.3 1.5 1.1 1.1 1.2 1.8 1.6 1.3 2.2 2.1 2.0 1.8 2.1 1.7
Intensity Change (Log2) 0.03 0.04 -0.25 -0.35 -0.33 -0.36 -0.46 -0.43 -0.41 0.4 0.28 0.11 0.44 0.4 0.34 0.34 0.47 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 0 0 0 0 0 -1.0 0 0 -0.4 -0.9 0 0 0 0.1
Bud 0.4 -1.1 -0.6 1.6 -0.5 1.7 0.6 2.2 0.6 0 0 0 -3.6 -2.5 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 1.3 0 0 0 1.4 1.5 0 0 0 1.2 1.5 0 0 0
Cytoplasm 1.4 5.5 6.6 6.9 6.6 5.8 4.4 0.7 2.1 -2.6 -1.7 1.0 4.5 5.6 3.7
Endoplasmic Reticulum -1.7 -4.7 -7.1 -9.1 -9.0 -7.8 -8.0 -5.7 -5.6 4.0 1.7 -0.2 -7.4 -6.8 -6.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.5 2.2 0 4.5 5.0 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.7 2.0 1.7 2.5 2.7 0 2.1 0 3.8 1.8 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.9579 10.848 11.1811 10.5947 9.3365 10.3511 8.119 10.3754 8.5869 8.6036 7.9471 9.2667 7.5398 9.0682 8.2581 7.9347 6.3484 7.6545
Actin 0.0055 0.0009 0.0037 0.0006 0.0006 0.0004 0.0105 0.0006 0.0096 0.0383 0.0127 0.0006 0.0149 0.0021 0.0086 0.0011 0.0066 0.0024
Bud 0.0001 0.0003 0.0002 0 0.0002 0.0001 0.0011 0.0003 0.0003 0.0006 0.0006 0.0001 0.0003 0.0002 0.0003 0.0002 0.0015 0.0002
Bud Neck 0.0002 0.0002 0.0005 0.0006 0.001 0.0038 0.0012 0.0007 0.0008 0.0008 0.0232 0.0018 0.0028 0.0013 0.0005 0.002 0.0005 0.0018
Bud Periphery 0.0001 0.0003 0.0002 0.0001 0.0001 0.0001 0.0018 0.0004 0.0004 0.0014 0.0009 0.0001 0.0005 0.0003 0.0004 0.0003 0.002 0.0002
Bud Site 0.0002 0.0024 0.0017 0.0001 0.0003 0.0001 0.0065 0.0023 0.0007 0.0012 0.0168 0.0001 0.0048 0.0005 0.0018 0.0041 0.0092 0.0004
Cell Periphery 0.0007 0.0014 0.0011 0.0007 0.0004 0.0003 0.0011 0.0035 0.0008 0.0122 0.0004 0.0005 0.0009 0.0016 0.0014 0.0008 0.0005 0.0004
Cytoplasm 0.1457 0.2534 0.1488 0.0781 0.2943 0.1963 0.1489 0.215 0.2073 0.1567 0.2154 0.1763 0.1173 0.2283 0.1064 0.1067 0.2505 0.1776
Cytoplasmic Foci 0.0098 0.0131 0.0126 0.0057 0.0208 0.0118 0.0185 0.0097 0.0111 0.0103 0.0093 0.0046 0.0138 0.0052 0.0221 0.0141 0.0086 0.0144
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0001 0.0003 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.7056 0.59 0.6896 0.7833 0.4571 0.6477 0.5637 0.5198 0.4768 0.3486 0.5328 0.5337 0.692 0.6272 0.6437 0.5177 0.4241 0.5774
Endosome 0.0554 0.0478 0.0643 0.0248 0.1002 0.072 0.0731 0.0495 0.0909 0.1058 0.0347 0.0781 0.0568 0.0396 0.0748 0.1668 0.0691 0.0853
Golgi 0.0029 0.0062 0.006 0.004 0.005 0.0042 0.0089 0.0039 0.0142 0.0165 0.0238 0.0013 0.0079 0.0034 0.0086 0.0101 0.0036 0.0045
Lipid Particles 0.0159 0.0371 0.0124 0.0269 0.016 0.0067 0.0069 0.0119 0.0129 0.0226 0.0057 0.004 0.0118 0.0087 0.0144 0.0109 0.0061 0.02
Mitochondria 0.0006 0.0004 0.0007 0.0002 0.0009 0.0005 0.0075 0.0008 0.0123 0.0078 0.0055 0.0005 0.0026 0.0004 0.0023 0.0133 0.0439 0.0095
None 0.0089 0.0017 0.0037 0.0004 0.0036 0.0027 0.0037 0.0007 0.001 0.0014 0.0005 0.0003 0.0037 0.0005 0.0011 0.0004 0.0014 0.0008
Nuclear Periphery 0.0234 0.0139 0.0136 0.0119 0.0242 0.0158 0.0663 0.0412 0.0435 0.0528 0.0664 0.0477 0.0348 0.0216 0.0243 0.0314 0.0617 0.0292
Nucleolus 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0004 0.0002 0.0003 0.0002 0.0019 0 0.0002 0 0.0001 0.0008 0.0005 0.0002
Nucleus 0.0026 0.0032 0.003 0.0012 0.0082 0.0029 0.0069 0.0051 0.0041 0.0047 0.0076 0.0065 0.0031 0.0023 0.002 0.0021 0.0143 0.0038
Peroxisomes 0.0005 0.0001 0.0002 0.0001 0.0004 0.0001 0.0018 0.0004 0.0011 0.0067 0.0018 0 0.0029 0.0001 0.0056 0.0042 0.001 0.0042
Punctate Nuclear 0.0007 0.0007 0.0018 0.0002 0.0013 0.0005 0.0041 0.0007 0.0015 0.0016 0.0059 0.0004 0.0019 0.0003 0.0013 0.0003 0.0024 0.0019
Vacuole 0.0191 0.0253 0.0334 0.0581 0.0612 0.0313 0.0574 0.1277 0.098 0.1938 0.0304 0.1336 0.0242 0.053 0.0746 0.1024 0.0846 0.0598
Vacuole Periphery 0.0022 0.0013 0.0024 0.003 0.004 0.0025 0.0091 0.0055 0.0123 0.0159 0.0037 0.0097 0.0027 0.0034 0.0059 0.0101 0.0079 0.0061

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.6771 32.4522 32.3191 30.6405 30.9656 42.0112 35.4028 25.3515 35.1367 41.851
Translational Efficiency 1.2081 1.116 0.9039 1.3648 1.8069 1.6696 1.0565 1.4401 1.3409 1.173

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
Localization
Cell Percentages ER (94%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Scs7

Scs7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Scs7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available